专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20100121044A1 SOLID PHASE TECHNIQUE FOR SELECTIVELY ISOLATING NUCLEIC ACIDS 审中-公开
标题翻译：用于选择性分离核酸的固相技术
公开(公告)号：US20100121044A1
公开(公告)日：2010-05-13
申请号：US12490674
申请日：2009-06-24
申请人： Kevin McKernan , Paul McEwan , William Morris
发明人： Kevin McKernan , Paul McEwan , William Morris
IPC分类号： C07H1/06
CPC分类号： C12N15/1013 , C12N15/1006 , C12Q1/6806 , C12Q2563/143 , C12Q2527/137 , C12Q2523/308
摘要： A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed. The disclosed method provides a simple, robust and readily automatable means of nucleic acid isolation and purification which produces high quality nucleic acid molecules suitable for: capillary electrophoresis, nucleotide sequencing, reverse transcription cloning the transfection, transduction or microinjection of mammalian cells, gene therapy protocols, the in vitro synthesis of RNA probes, cDNA library construction and PCR amplification.
摘要翻译：在存在或不存在其它生物分子的情况下，通过选择性促进特定种类的核酸分子吸附到官能团涂覆的表面上，从包含不同大小的核酸分子的混合物的溶液中分离靶核酸分子的方法的磁响应顺磁性微粒。通过操作溶液的离子强度和聚亚烷基二醇浓度来选择性地沉淀并可逆吸附具有特定分子尺寸的特征的核酸分子的靶物种到顺磁性微粒，其表面作为生物亲和力用于核酸的吸附剂。基于分子大小和去除已经吸附了靶核酸分子的磁珠，从起始混合物中分离靶核酸。所公开的方法提供了简单，稳健且易于自动化的核酸分离和纯化手段，其产生适合于毛细管电泳，核苷酸测序，逆转录克隆转染，转导或显微注射哺乳动物细胞的高质量核酸分子，基因治疗方案，RNA探针的体外合成，cDNA文库构建和PCR扩增。

2. 发明申请

US20060003357A1 Solid phase technique for selectively isolating nucleic acids 审中-公开
公开(公告)号：US20060003357A1
公开(公告)日：2006-01-05
申请号：US11129218
申请日：2005-05-13
申请人： Kevin McKernan , Paul McEwan , William Morris
发明人： Kevin McKernan , Paul McEwan , William Morris
IPC分类号： C12Q1/68 , C12N1/08
CPC分类号： C12N15/1013 , C12N15/1006 , C12Q1/6806 , C12Q2563/143 , C12Q2527/137 , C12Q2523/308
摘要： A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed. The disclosed method provides a simple, robust and readily automatable means of nucleic acid isolation and purification which produces high quality nucleic acid molecules suitable for: capillary electrophoresis, nucleotide sequencing, reverse transcription cloning the transfection, transduction or microinjection of mammalian cells, gene therapy protocols, the in vitro synthesis of RNA probes, cDNA library construction and PCR amplification.

3. 发明授权

US06534262B1 Solid phase technique for selectively isolating nucleic acids 有权
标题翻译：用于选择性分离核酸的固相技术
公开(公告)号：US06534262B1
公开(公告)日：2003-03-18
申请号：US09311317
申请日：1999-05-13
申请人： Kevin McKernan , Paul McEwan , William Morris
发明人： Kevin McKernan , Paul McEwan , William Morris
IPC分类号： C12Q168
CPC分类号： C12N15/1013 , C12N15/1006 , C12Q1/6806 , C12Q2563/143 , C12Q2527/137 , C12Q2523/308
摘要： A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed. The disclosed method provides a simple, robust and readily automatable means of nucleic acid isolation and purification which produces high quality nucleic acid molecules suitable for: capillary electrophoresis, nucleotide sequencing, reverse transcription cloning the transfection, transduction or microinjection of mammalian cells, gene therapy protocols, the in vitro synthesis of RNA probes, cDNA library construction and PCR amplification.
摘要翻译：在存在或不存在其它生物分子的情况下，通过选择性促进特定种类的核酸分子吸附到官能团涂覆的表面上，从包含不同大小的核酸分子的混合物的溶液中分离靶核酸分子的方法的磁响应顺磁性微粒。通过操作溶液的离子强度和聚亚烷基二醇浓度来选择性地沉淀并可逆吸附具有特定分子尺寸的特征的核酸分子的靶物种到顺磁性微粒，其表面作为生物亲和力用于核酸的吸附剂。基于分子大小和去除已经吸附了靶核酸分子的磁珠，从起始混合物中分离靶核酸。所公开的方法提供了简单，稳健且易于自动化的核酸分离和纯化手段，其产生适合于毛细管电泳，核苷酸测序，逆转录克隆转染，转导或显微注射哺乳动物细胞的高质量核酸分子，基因治疗方案，RNA探针的体外合成，cDNA文库构建和PCR扩增。

4. 发明授权

US10457968B2 Modified type A DNA polymerases 有权
公开(公告)号：US10457968B2
公开(公告)日：2019-10-29
申请号：US13061940
申请日：2009-11-03
申请人： William Bourn , Maryke Appel , Gavin Rush , John Foskett , Paul McEwan
发明人： William Bourn , Maryke Appel , Gavin Rush , John Foskett , Paul McEwan
IPC分类号： C12N9/12 , C12P19/34 , C12N15/10 , C12Q1/48
摘要： The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

5. 发明申请

US20110281305A1 MODIFIED TYPE A DNA POLYMERASES 审中-公开
标题翻译：修饰型A DNA聚合酶
公开(公告)号：US20110281305A1
公开(公告)日：2011-11-17
申请号：US13061940
申请日：2009-11-03
申请人： William Bourn , Maryke Appel , Gavin Rush , John Foskett , Paul McEwan
发明人： William Bourn , Maryke Appel , Gavin Rush , John Foskett , Paul McEwan
IPC分类号： C12P19/34 , C12N15/70 , C12N1/21 , C12N9/12
CPC分类号： C12P19/34 , C12N9/1241 , C12N9/1252 , C12N15/1058 , C12Q1/485 , C12Y207/07007 , G01N2333/9126
摘要： The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
摘要翻译：本发明提供改进的DNA聚合酶，特别是A型DNA聚合酶，其可以更适合于重组DNA技术中的应用。除此之外，本发明提供了衍生自定向进化实验的修饰的DNA聚合酶，设计用于选择在工业或研究应用中使用的条件下赋予有利表型的突变。

6. 发明申请

US20140030765A1 MODIFIED DNA POLYMERASES FOR IMPROVED AMPLIFICATION 有权
标题翻译：用于改善放大的改性DNA聚合物
公开(公告)号：US20140030765A1
公开(公告)日：2014-01-30
申请号：US13979509
申请日：2012-01-13
申请人： Wolfgang Schafer , Paul McEwan , Eric Van Der Walt , John Foskett , William Bourn
发明人： Wolfgang Schafer , Paul McEwan , Eric Van Der Walt , John Foskett , William Bourn
IPC分类号： C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/686 , C12Y207/07007 , Y02P20/52
摘要： The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
摘要翻译：本发明提供了可以更好地适用于重组DNA技术，特别是涉及植物衍生样品的技术的改进的DNA聚合酶。除此之外，本发明提供了衍生自定向进化实验的修饰的DNA聚合酶，设计用于选择在工业或研究应用中使用的条件下赋予有利表型的突变。

7. 发明申请

US20120115188A1 CHIMERIC DNA POLYMERASES 有权
公开(公告)号：US20120115188A1
公开(公告)日：2012-05-10
申请号：US13127420
申请日：2009-11-03
申请人： Bjarne Faurholm , Paul McEwan , William Bourn , Gavin Rush
发明人： Bjarne Faurholm , Paul McEwan , William Bourn , Gavin Rush
IPC分类号： C12P19/34 , C12N1/21 , C07H21/00 , C12N9/10 , C12N15/63
CPC分类号： C12N9/1252 , C12N9/1241 , C12P19/34
摘要： The present invention provides, among other things, chimeric DNA polymerases containing heterologous domains having sequences derived from at least two DNA polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, PCR additives (e.g., PCR enhancers) and other impurities; and high fidelity) of different DNA polymerases in a chimeric polymerase.
摘要翻译：本发明尤其提供了含有异源结构域的嵌合DNA聚合酶，所述异源结构域具有源自至少两种DNA聚合酶的序列，其具有至少一个不同的功能特征（例如伸长率，持续性，错误率或保真度，耐盐性或抗性）以及制作和使用它们的方法。在一些实施方案中，本发明可以将不同DNA聚合酶的所需功能特征（例如，高延展率，高延伸率，耐热性，耐盐性，PCR添加剂（例如PCR增强剂）以及其他杂质以及高保真度）结合在一起嵌合聚合酶。

8. 发明授权

US09315787B2 Modified DNA polymerases for improved amplification 有权
标题翻译：改良的DNA聚合酶用于改善扩增
公开(公告)号：US09315787B2
公开(公告)日：2016-04-19
申请号：US13979509
申请日：2012-01-13
申请人： Wolfgang Schafer , Paul McEwan , Eric Van Der Walt , John Foskett , William Bourn
发明人： Wolfgang Schafer , Paul McEwan , Eric Van Der Walt , John Foskett , William Bourn
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/686 , C12Y207/07007 , Y02P20/52
摘要： The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
摘要翻译：本发明提供了可以更好地适用于重组DNA技术，特别是涉及植物衍生样品的技术的改进的DNA聚合酶。除此之外，本发明提供了衍生自定向进化实验的修饰的DNA聚合酶，设计用于选择在工业或研究应用中使用的条件下赋予有利表型的突变。

9. 发明授权

US09023633B2 Chimeric DNA polymerases 有权
标题翻译：嵌合DNA聚合酶
公开(公告)号：US09023633B2
公开(公告)日：2015-05-05
申请号：US13127420
申请日：2009-11-03
申请人： Bjarne Faurholm , Paul McEwan , William Bourn , Gavin Rush
发明人： Bjarne Faurholm , Paul McEwan , William Bourn , Gavin Rush
IPC分类号： C12N9/00 , C12N9/12 , C12P19/34
CPC分类号： C12N9/1252 , C12N9/1241 , C12P19/34
摘要： The present invention provides, among other things, chimeric DNA polymerases containing heterologous domains having sequences derived from at least two DNA polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, PCR additives (e.g., PCR enhancers) and other impurities; and high fidelity) of different DNA polymerases in a chimeric polymerase.
摘要翻译：本发明尤其提供了含有异源结构域的嵌合DNA聚合酶，所述异源结构域具有源自至少两种DNA聚合酶的序列，其具有至少一个不同的功能特征（例如伸长率，持续性，错误率或保真度，耐盐性或抗性）以及制作和使用它们的方法。在一些实施方案中，本发明可以将不同DNA聚合酶的所需功能特征（例如，高延展率，高延伸率，耐热性，耐盐性，PCR添加剂（例如PCR增强剂）以及其他杂质以及高保真度）结合在一起嵌合聚合酶。

10. 发明申请

US20110294167A1 NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：核酸放大
公开(公告)号：US20110294167A1
公开(公告)日：2011-12-01
申请号：US13132305
申请日：2009-12-02
申请人： Paul McEwan , Bjarne Faurholm , Eric Van Der Walt
发明人： Paul McEwan , Bjarne Faurholm , Eric Van Der Walt
IPC分类号： C12P19/34 , C12N9/12
CPC分类号： C12Q1/6844 , C12Q2527/125
摘要： The present invention provides improved systems and methods for amplifying nucleic acids. Among other things the present invention provides a system for amplifying nucleic acids through use of a primase and a polymerase with strand-displacement ability without, for example, exogenously-added primers. The present invention is particularly useful for whole genome amplification.
摘要翻译：本发明提供了用于扩增核酸的改进的系统和方法。其中本发明提供了一种通过使用引物酶和具有链置换能力的聚合酶来扩增核酸的系统，而不需要例如外源性加入的引物。本发明对于全基因组扩增特别有用。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式