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    • 1. 发明申请
    • TRANSFORMANT TRANSFECTED WITH FLAVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE GENE AND METHOD FOR PRODUCING FLAVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE USING THE SAME
    • 用FLAVIN ADENINE DINENTRY-BINDING GLUCOSE DEHYDROGENASE基因转染的转化体及其生产FlaVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE的方法
    • US20110053194A1
    • 2011-03-03
    • US12745342
    • 2008-10-03
    • Kensuke YuukiYuji Nakanishi
    • Kensuke YuukiYuji Nakanishi
    • C12Q1/54C12N1/15C12N1/19C12N9/04
    • C12Q1/32C12N9/0006C12Q1/006C12Q1/54
    • It is intended to highly efficiently produce a large amount of novel FAD-GDH capable of more accurately measuring a glucose level. Provided is a transformant in which a DNA encoding a flavin adenine dinucleotide-binding glucose dehydrogenase selected from the group consisting of (a) a DNA encoding the amino acid sequence of SEQ ID NO: 20; (b) a DNA consisting of the base sequence of SEQ ID NO: 19; (c) a DNA having a base sequence homologous to the base sequence of SEQ ID NO: 19 and encoding a protein having a flavin adenine dinucleotide-binding glucose dehydrogenase activity; (d) a DNA encoding the amino acid sequence of SEQ ID NO: 34; (e) a DNA consisting of the base sequence of SEQ ID NO: 33; and (f) a DNA having a base sequence homologous to the base sequence of SEQ ID NO: 33 and encoding a protein having a flavin adenine dinucleotide-binding glucose dehydrogenase activity has been introduced. Further, a method for producing a flavin adenine dinucleotide-binding glucose dehydrogenase using the transformant is provided.
    • 旨在高效地产生能够更准确地测量葡萄糖水平的大量新型FAD-GDH。 本发明提供编码黄曲霉腺嘌呤二核苷酸结合葡萄糖脱氢酶的DNA的转化体,其选自(a)编码SEQ ID NO:20的氨基酸序列的DNA; (b)由SEQ ID NO:19的碱基序列组成的DNA; (c)具有与SEQ ID NO:19的碱基序列同源的碱基序列并编码具有黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶活性的蛋白质的DNA; (d)编码SEQ ID NO:34的氨基酸序列的DNA; (e)由SEQ ID NO:33的碱基序列组成的DNA; 并且(f)已经引入了具有与SEQ ID NO:33的碱基序列同源的碱基序列并编码具有黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶活性的蛋白质的DNA。 此外,提供了使用转化体生产黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶的方法。
    • 2. 发明申请
    • Flavin Adenine Dinucleotide-Binding Glucose Dehydrogenase
    • 黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶
    • US20090181408A1
    • 2009-07-16
    • US12227791
    • 2007-05-25
    • Noriaki TanakaKensuke Yuuki
    • Noriaki TanakaKensuke Yuuki
    • C12N9/02C12N1/14C12N1/15C12N15/11C12N15/52C12N15/80C12P21/02G01N33/53
    • C12R1/69C12N9/0006C12Q1/54
    • The object is to provide a novel enzyme which enables to determine the glucose level more accurately, a bacterium capable of producing the enzyme, and use of the enzyme. Disclosed is a flavin adenine dinucleotide-binding glucose dehydrogenase having the following properties (1) to (3): (1) the enzyme has an activity of catalyzing a reaction for oxidizing a hydroxyl group in glucose in the presence of an electron acceptor to produce glucono-δ-lactone; (2) the enzyme has a molecular weight of about 100 kDa as measured by SDS-polyacrylamide gel electrophoresis or about 400 kDa as measured by gel filtration chromatography; and (3) the enzyme is less reactive to maltose, D-fructose, D-mannose and D-galactose. Also disclosed is a microorganism Aspergillus oryzae which can produce the enzyme. Further disclosed are a glucose determination method, a reagent for the determination of glucose, and a kit for the determination of glucose, each utilizing the enzyme.
    • 本发明的目的是提供一种能够更准确地测定葡萄糖水平,能够产生酶的细菌和使用该酶的新型酶。 公开了具有以下性质(1)至(3)的黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶:(1)酶在电子受体存在下具有催化葡萄糖氧化反应的反应活性,以产生 葡萄糖酸-δ-内酯; (2)通过SDS-聚丙烯酰胺凝胶电泳测定的酶的分子量为约100kDa,或通过凝胶过滤层析测定的约400kDa的酶; 和(3)酶对麦芽糖,D-果糖,D-甘露糖和D-半乳糖的反应性较差。 还公开了可以产生酶的微生物米曲霉。 进一步公开了葡萄糖测定方法,用于测定葡萄糖的试剂和用于测定葡萄糖的试剂盒,各自使用酶。