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1. 发明申请

US20110053194A1 TRANSFORMANT TRANSFECTED WITH FLAVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE GENE AND METHOD FOR PRODUCING FLAVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE USING THE SAME 审中-公开
标题翻译：用FLAVIN ADENINE DINENTRY-BINDING GLUCOSE DEHYDROGENASE基因转染的转化体及其生产FlaVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE的方法
公开(公告)号：US20110053194A1
公开(公告)日：2011-03-03
申请号：US12745342
申请日：2008-10-03
申请人： Kensuke Yuuki , Yuji Nakanishi
发明人： Kensuke Yuuki , Yuji Nakanishi
IPC分类号： C12Q1/54 , C12N1/15 , C12N1/19 , C12N9/04
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/006 , C12Q1/54
摘要： It is intended to highly efficiently produce a large amount of novel FAD-GDH capable of more accurately measuring a glucose level. Provided is a transformant in which a DNA encoding a flavin adenine dinucleotide-binding glucose dehydrogenase selected from the group consisting of (a) a DNA encoding the amino acid sequence of SEQ ID NO: 20; (b) a DNA consisting of the base sequence of SEQ ID NO: 19; (c) a DNA having a base sequence homologous to the base sequence of SEQ ID NO: 19 and encoding a protein having a flavin adenine dinucleotide-binding glucose dehydrogenase activity; (d) a DNA encoding the amino acid sequence of SEQ ID NO: 34; (e) a DNA consisting of the base sequence of SEQ ID NO: 33; and (f) a DNA having a base sequence homologous to the base sequence of SEQ ID NO: 33 and encoding a protein having a flavin adenine dinucleotide-binding glucose dehydrogenase activity has been introduced. Further, a method for producing a flavin adenine dinucleotide-binding glucose dehydrogenase using the transformant is provided.
摘要翻译：旨在高效地产生能够更准确地测量葡萄糖水平的大量新型FAD-GDH。本发明提供编码黄曲霉腺嘌呤二核苷酸结合葡萄糖脱氢酶的DNA的转化体，其选自（a）编码SEQ ID NO：20的氨基酸序列的DNA; （b）由SEQ ID NO：19的碱基序列组成的DNA; （c）具有与SEQ ID NO：19的碱基序列同源的碱基序列并编码具有黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶活性的蛋白质的DNA; （d）编码SEQ ID NO：34的氨基酸序列的DNA; （e）由SEQ ID NO：33的碱基序列组成的DNA; 并且（f）已经引入了具有与SEQ ID NO：33的碱基序列同源的碱基序列并编码具有黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶活性的蛋白质的DNA。此外，提供了使用转化体生产黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶的方法。

2. 发明申请

US20090181408A1 Flavin Adenine Dinucleotide-Binding Glucose Dehydrogenase 审中-公开
标题翻译：黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶
公开(公告)号：US20090181408A1
公开(公告)日：2009-07-16
申请号：US12227791
申请日：2007-05-25
申请人： Noriaki Tanaka , Kensuke Yuuki
发明人： Noriaki Tanaka , Kensuke Yuuki
IPC分类号： C12N9/02 , C12N1/14 , C12N1/15 , C12N15/11 , C12N15/52 , C12N15/80 , C12P21/02 , G01N33/53
CPC分类号： C12R1/69 , C12N9/0006 , C12Q1/54
摘要： The object is to provide a novel enzyme which enables to determine the glucose level more accurately, a bacterium capable of producing the enzyme, and use of the enzyme. Disclosed is a flavin adenine dinucleotide-binding glucose dehydrogenase having the following properties (1) to (3): (1) the enzyme has an activity of catalyzing a reaction for oxidizing a hydroxyl group in glucose in the presence of an electron acceptor to produce glucono-δ-lactone; (2) the enzyme has a molecular weight of about 100 kDa as measured by SDS-polyacrylamide gel electrophoresis or about 400 kDa as measured by gel filtration chromatography; and (3) the enzyme is less reactive to maltose, D-fructose, D-mannose and D-galactose. Also disclosed is a microorganism Aspergillus oryzae which can produce the enzyme. Further disclosed are a glucose determination method, a reagent for the determination of glucose, and a kit for the determination of glucose, each utilizing the enzyme.
摘要翻译：本发明的目的是提供一种能够更准确地测定葡萄糖水平，能够产生酶的细菌和使用该酶的新型酶。公开了具有以下性质（1）至（3）的黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶：（1）酶在电子受体存在下具有催化葡萄糖氧化反应的反应活性，以产生葡萄糖酸-δ-内酯; （2）通过SDS-聚丙烯酰胺凝胶电泳测定的酶的分子量为约100kDa，或通过凝胶过滤层析测定的约400kDa的酶; 和（3）酶对麦芽糖，D-果糖，D-甘露糖和D-半乳糖的反应性较差。还公开了可以产生酶的微生物米曲霉。进一步公开了葡萄糖测定方法，用于测定葡萄糖的试剂和用于测定葡萄糖的试剂盒，各自使用酶。

3. 发明申请

US20080248524A1 AMP Deaminase Originating Streptomyces And Utilization Thereof 有权
标题翻译： AMP脱氨酶起源链霉菌及其利用
公开(公告)号：US20080248524A1
公开(公告)日：2008-10-09
申请号：US11587947
申请日：2005-04-26
申请人： Ryoko Mizuguchi , Shigeharu Mori , Atsuki Toumoto , Kei-ichi Ando , Kensuke Yuuki
发明人： Ryoko Mizuguchi , Shigeharu Mori , Atsuki Toumoto , Kei-ichi Ando , Kensuke Yuuki
IPC分类号： C12P21/04 , C12N9/78 , C12P1/00 , C12N15/11 , C12N15/00 , C12N1/20
CPC分类号： C12P19/32 , C12N9/78
摘要： It is intended to provide a thermostable AMP deaminase originating in a microorganism. Namely, an AMP deaminase having the following characteristics. (1) Catalyzing the reaction: 5′-adenylic acid+H2O→5′-inosinic acid+NH3; (2) being stable at a temperature of 65° C. or below (in an acetate buffer (pH 5.6)); (3) having a molecular weight of 48,000±2,000 in gel filtration; and (4) having the optimum pH value at around 5.6 (in McIlvaine buffer).
摘要翻译：旨在提供源自微生物的热稳定AMP脱氨酶。即，具有以下特征的AMP脱氨酶。（1）催化反应：5'-腺苷酸+ H 2 O 5→5'-肌苷酸+ NH 3 （2）在65℃或更低的温度（在乙酸盐缓冲液（pH5.6））中稳定）; （3）在凝胶过滤中分子量为48,000±2,000; 和（4）具有约5.6的最佳pH值（麦克林蛋白缓冲液）。

4. 发明授权

US07709240B2 AMP deaminase originating streptomyces and utilization thereof 有权
标题翻译： AMP脱氨酶起源于链霉菌及其利用
公开(公告)号：US07709240B2
公开(公告)日：2010-05-04
申请号：US11587947
申请日：2005-04-26
申请人： Ryoko Mizuguchi , Shigeharu Mori , Atsuki Toumoto , Kei-ichi Ando , Kensuke Yuuki
发明人： Ryoko Mizuguchi , Shigeharu Mori , Atsuki Toumoto , Kei-ichi Ando , Kensuke Yuuki
IPC分类号： C12N9/14 , C12Q1/00 , C12Q1/68 , C12Q1/34 , C12N1/20 , C12N15/00 , C12P21/04 , C07H21/04
CPC分类号： C12P19/32 , C12N9/78
摘要： It is intended to provide a thermostable AMP deaminase originating in a microorganism. Namely, an AMP deaminase having the following characteristics. (1) Catalyzing the reaction: 5′-adenylic acid+H2O→5′-inosinic acid+NH3; (2) being stable at a temperature of 65° C. or below (in an acetate buffer (pH 5.6)); (3) having a molecular weight of 48,000±2,000 in gel filtration; and (4) having the optimum pH value at around 5.6 (in McIlvaine buffer).
摘要翻译：旨在提供源自微生物的热稳定AMP脱氨酶。即，具有以下特征的AMP脱氨酶。（1）催化反应：5'-腺苷酸+ H 2 O→5'-肌苷酸+ NH 3; （2）在65℃或更低的温度（在乙酸盐缓冲液（pH5.6））中稳定）; （3）在凝胶过滤中分子量为48,000±2,000; 和（4）具有约5.6的最佳pH值（麦克林蛋白缓冲液）。

5. 发明授权

US07348170B2 Fungus-origin lysyl oxidases 失效
标题翻译：真菌来源的赖氨酰氧化酶
公开(公告)号：US07348170B2
公开(公告)日：2008-03-25
申请号：US10498517
申请日：2002-12-25
申请人： Kensuke Yuuki , Atsuki Toumoto , Masayuki Machida , Keietsu Abe , Katsuya Gomi , Kiyoshi Asai , Motoaki Sano , Taishin Kin , Hideki Nagasaki , Akira Hosoyama , Osamu Akita , Naotake Ogasawara , Satoru Hisahara
发明人： Kensuke Yuuki , Atsuki Toumoto , Masayuki Machida , Keietsu Abe , Katsuya Gomi , Kiyoshi Asai , Motoaki Sano , Taishin Kin , Hideki Nagasaki , Akira Hosoyama , Osamu Akita , Naotake Ogasawara , Satoru Hisahara
IPC分类号： C12N9/02 , C12N15/00 , C12N1/00 , C12P21/06 , C07H21/04
CPC分类号： C12N9/80 , C12N9/0022 , C12N9/48 , C12P21/06
摘要： Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.
摘要翻译：提供衍生自丝状真菌的赖氨酰氧化酶及其编码的DNA。包含以下（a）或（b）所述的蛋白质的赖氨酰氧化酶：（a）具有SEQ ID NO：2所示的氨基酸序列的蛋白质; 或（b）具有通过修饰SEQ ID NO：2所示氨基酸序列的一部分而作为赖氨酰氧化酶起作用的氨基酸序列的蛋白质。

6. 发明授权

US07329521B2 Transglutaminase-producing strain 有权
标题翻译：转谷氨酰胺酶生产菌株
公开(公告)号：US07329521B2
公开(公告)日：2008-02-12
申请号：US10527090
申请日：2003-09-09
申请人： Kensuke Yuuki , Kinya Washizu
发明人： Kensuke Yuuki , Kinya Washizu
IPC分类号： C12N1/20 , C12N9/00 , C12N9/10 , C12N15/70 , C12P21/06 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/1044
摘要： It is intended to provide a strain capable of producing transglutaminase at a high efficiency; and a process for producing transglutaminase by using this strain. A structural gene from Streptomyces mobaraensis and a promoter and a terminator acting on this structural gene are externally transferred into Streptomyces mobaraensis to give a transformant. Transglutaminase is produced by culturing this transformant.
摘要翻译：旨在提供能够以高效率生产转谷氨酰胺酶的菌株; 以及通过使用该菌株生产转谷氨酰胺酶的方法。来自动物链霉菌（Streptomyces mobaraensis）的结构基因和作用于该结构基因的启动子和终止子外部转移到动物链霉菌（Streptomyces mobaraensis）中以产生转化体。转谷氨酰胺酶是通过培养该转化体产生的。

7. 发明申请

US20060035366A1 Transglutaminase-producing strain 有权
标题翻译：转谷氨酰胺酶生产菌株
公开(公告)号：US20060035366A1
公开(公告)日：2006-02-16
申请号：US10527090
申请日：2003-09-09
申请人： Kensuke Yuuki , Kinya Washizu
发明人： Kensuke Yuuki , Kinya Washizu
IPC分类号： C12N1/21 , C07H21/04 , C12P21/06 , C12N9/10 , C12N15/74
CPC分类号： C12N9/1044
摘要： It is intended to provide a strain capable of producing transglutaminase at a high efficiency; and a process for producing transglutaminase by using this strain. A structural gene from Streptomyces mobaraensis and a promoter and a terminator acting on this structural gene are externally transferred into Streptomyces mobaraensis to give a transformant. Transglutaminase is produced by culturing this transformant.
摘要翻译：旨在提供能够以高效率生产转谷氨酰胺酶的菌株; 以及通过使用该菌株生产转谷氨酰胺酶的方法。来自动物链霉菌（Streptomyces mobaraensis）的结构基因和作用于该结构基因的启动子和终止子外部转移到动物链霉菌（Streptomyces mobaraensis）中以产生转化体。转谷氨酰胺酶是通过培养该转化体产生的。

8. 发明申请

US20050142650A1 Fungus-origin lysyl oxidases 失效
标题翻译：真菌来源的赖氨酰氧化酶
公开(公告)号：US20050142650A1
公开(公告)日：2005-06-30
申请号：US10498517
申请日：2002-12-25
申请人： Kensuke Yuuki , Atsuki Toumoto , Masayuki Machida , Katsuya Gomi , Kiyoshi Asai , Motoaki Sano , Taishin Kin , Hideki Nagasaki , Akira Hosoyama , Osamu Akita , Naotake Ogasawara , Satoru Kuhara
发明人： Kensuke Yuuki , Atsuki Toumoto , Masayuki Machida , Katsuya Gomi , Kiyoshi Asai , Motoaki Sano , Taishin Kin , Hideki Nagasaki , Akira Hosoyama , Osamu Akita , Naotake Ogasawara , Satoru Kuhara
IPC分类号： C12N1/15 , C12N1/19 , C12N1/21 , C12N9/06 , C12N9/48 , C12N9/62 , C12N9/80 , C12N15/52 , C12N15/53 , C12N15/57 , C12P21/06 , C07H21/04 , C12N1/16 , C12N1/18 , C12N15/74
CPC分类号： C12N9/80 , C12N9/0022 , C12N9/48 , C12P21/06
摘要： Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.
摘要翻译：提供衍生自丝状真菌的赖氨酰氧化酶及其编码的DNA。包含以下（a）或（b）所述的蛋白质的赖氨酰氧化酶：（a）具有SEQ ID NO：2所示的氨基酸序列的蛋白质; 或（b）具有通过修饰SEQ ID NO：2所示氨基酸序列的一部分而作为赖氨酰氧化酶起作用的氨基酸序列的蛋白质。

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