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1. 发明申请

US20080044897A1 GENE SILENCING VECTOR AND GENE SILENCING METHOD USING THE SAME 审中-公开
标题翻译：基因沉默载体和基因沉默方法
公开(公告)号：US20080044897A1
公开(公告)日：2008-02-21
申请号：US11874829
申请日：2007-10-18
申请人： Keisuke Kasaoka , Yasuhito Saito , Shigeru Kuwata
发明人： Keisuke Kasaoka , Yasuhito Saito , Shigeru Kuwata
IPC分类号： C12N15/00
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8283
摘要： A gene silencing vector according to the present invention comprises a vector which includes a promoter, an enhancer sequence in the downstream of the promoter, and a gene encoding a potyvirus-origin coat protein in the downstream of the enhancer sequence, wherein in order to cause gene silencing of the specific target gene in a host plant, the vector is used with a specific target gene or a gene that is homologous to the target gene inserted in the vicinity of the gene encoding the coat protein.
摘要翻译：根据本发明的基因沉默载体包含载体，其包含启动子，启动子下游的增强子序列和编码加强子序列下游的病毒源外壳蛋白的基因，其中为了引起在宿主植物中特异性靶基因的基因沉默，该载体与特异性靶基因或与插入编码外壳蛋白的基因附近的靶基因同源的基因使用。

2. 发明申请

US20050132440A1 Gene silencing vector and gene silencing method using the same 审中-公开
标题翻译：基因沉默载体和使用其的基因沉默方法
公开(公告)号：US20050132440A1
公开(公告)日：2005-06-16
申请号：US10992670
申请日：2004-11-22
申请人： Keisuke Kasaoka , Yasuhito Saito , Shigeru Kuwata
发明人： Keisuke Kasaoka , Yasuhito Saito , Shigeru Kuwata
IPC分类号： C12N15/82 , A01H1/00
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8283
摘要： A gene silencing vector according to the present invention comprises a vector which includes a promoter, an enhancer sequence in the downstream of the promoter, and a gene encoding a potyvirus-origin coat protein in the downstream of the enhancer sequence, wherein in order to cause gene silencing of the specific target gene in a host plant, the vector is used with a specific target gene or a gene that is homologous to the target gene inserted in the vicinity of the gene encoding the coat protein.
摘要翻译：根据本发明的基因沉默载体包含载体，其包含启动子，启动子下游的增强子序列和编码加强子序列下游的病毒源外壳蛋白的基因，其中为了引起在宿主植物中特异性靶基因的基因沉默，该载体与特异性靶基因或与插入编码外壳蛋白的基因附近的靶基因同源的基因使用。

3. 发明授权

US06228637B1 Recombinant vector, method for giving immunity against PVY-T to potato plant, and potato plant having immunity against PVY-T 失效
标题翻译：重组载体，对PVY-T免疫马铃薯植物的方法和具有抗PVY-T免疫力的马铃薯植株
公开(公告)号：US06228637B1
公开(公告)日：2001-05-08
申请号：US08558935
申请日：1995-11-13
申请人： Keisuke Kasaoka , Naoto Kadotani , Shigeru Kuwata , Yumiko Hayashi
发明人： Keisuke Kasaoka , Naoto Kadotani , Shigeru Kuwata , Yumiko Hayashi
IPC分类号： C12N1500
CPC分类号： C07K14/005 , A01H5/04 , C12N15/8283 , C12N2770/40022
摘要： A recombinant vector which can transform potato plants so as to confer upon the potato plant immunity against potato virus Y necrosis line is disclosed. The recombinant vector according to the present invention comprises a promoter which functions in a potato plant cell; an operably linked leader sequence of RNA4 of cucumber mosaic virus, which is located downstream of the promoter; and an operably linked sequence which encodes a coat protein of potato virus Y necrosis line, which is located downstream of said leader sequence; and the recombinant vector is capable of transforming potato plants.
摘要翻译：公开了能够转化马铃薯植物以赋予马铃薯植株免疫马铃薯病毒Y坏死系的重组载体。根据本发明的重组载体包含在马铃薯植物细胞中起作用的启动子; 位于启动子下游的黄瓜花叶病毒RNA4的可操作连接的前导序列; 和可操作地连接的序列，其编码位于所述前导序列下游的马铃薯病毒Y坏死系的外壳蛋白; 重组载体能够转化马铃薯植物。

4. 发明授权

US07525014B2 Disease-resistant plants and method of constructing the same 失效
标题翻译：抗病植物及其构建方法
公开(公告)号：US07525014B2
公开(公告)日：2009-04-28
申请号：US10363832
申请日：2001-09-07
申请人： Yoshimitsu Takakura , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
发明人： Yoshimitsu Takakura , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
IPC分类号： C12N15/09 , C12N15/31 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8282 , C07K14/21
摘要： It is the object of the present invention to provide disease-resistant plants which have been transformed to cause an effective defense reaction, and methods for producing the same.The present invention provides expression cassettes comprising a promoter capable of promoting a constitutive, inducible, or organ- or phase-specific gene expression, and a gene, under the control of said promoter, encoding an elicitor protein.
摘要翻译：本发明的目的是提供已经转化以产生有效防御反应的抗病植物及其制备方法。本发明提供了包含能够促进组成型，诱导型或器官或相位特异性基因表达的启动子的表达盒，以及在所述启动子控制下编码引发蛋白的基因。

5. 发明授权

US07232673B2 Antimicrobial protein from Lyophyllum shimeji 失效
标题翻译：来自Lyophyllum shimeji的抗菌蛋白
公开(公告)号：US07232673B2
公开(公告)日：2007-06-19
申请号：US11033116
申请日：2005-01-12
申请人： Yoshimitsu Takakura , Shigeru Kuwata , Yasuhiro Inoue
发明人： Yoshimitsu Takakura , Shigeru Kuwata , Yasuhiro Inoue
IPC分类号： C12N9/98 , C12N9/00
CPC分类号： C07K14/375 , A01N63/04 , C12Q1/689
摘要： The object of the present invention is to screen and identify a novel antimicrobial protein which can inhibit the growth of plant pathogenic microorganisms at a relatively low concentration such as Pyricularia oryzae and Rhizoctonia solani causative of two major diseases causing damage to rice crops and, further, to clone the gene of this protein. According to the present invention, an antimicrobial protein which can be obtained from a fraction of an aqueous extract of Lyophyllum shimeji precipitated by the ammonium sulfate precipitation method, has an antimicrobial activity at least against Rhizoctonia solani or Pyricularia oryzae, and shows the presence of components of about 70 kDa and/or about 65 kDa in molecular weight in the SDS-PAGE method. A gene encoding this protein and a method of using the same are provided.
摘要翻译：本发明的目的是筛选和鉴定一种新的抗微生物蛋白质，其可以以相对低的浓度抑制植物致病微生物的生长，例如造成稻谷作物损害的两种主要疾病的稻瘟病菌和立枯丝核菌属，克隆该蛋白质的基因。根据本发明，可以通过硫酸铵沉淀法沉淀出的一部分水杨酸提取物获得的抗微生物蛋白质具有抗菌活性，至少针对立枯丝核菌（Rhizoctonia solani）或Pyricularia oryzae，并显示了组分在SDS-PAGE方法中分子量约70kDa和/或约65kDa。提供了编码该蛋白质的基因及其使用方法。

6. 发明授权

US07851671B2 Disease-resistant plants and method of constructing the same 失效
标题翻译：抗病植物及其构建方法
公开(公告)号：US07851671B2
公开(公告)日：2010-12-14
申请号：US12406502
申请日：2009-03-18
申请人： Yoshimitsu Takakura , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
发明人： Yoshimitsu Takakura , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
IPC分类号： A01H5/00 , C12N15/09 , C12N15/82 , C12N15/31
CPC分类号： C12N15/8282 , C07K14/21
摘要： The present invention provides transgenic, disease-resistant plants which have been transformed with an expression cassette including a constitutive gene expression promoter; and a gene, under the control of the promoter, encoding a harpin. The transformed cells in the transgenic plant effect the constitutive expression of the harpin in an amount effective for inducing a defense reaction. The harpin is a protein consisting of an amino acid sequence that is at least 90% homologous to the amino acid sequence of SEQ ID NO: 2, and possesses a hypersensitive-response-inducing activity. The present invention also provides methods for producing the transgenic plants, expression cassettes, recombinant vectors and genes encoding harpins.
摘要翻译：本发明提供已经用包含组成型基因表达启动子的表达盒转化的转基因抗病植物; 和在启动子控制下编码harpin的基因。转基因植物中的转化细胞以有效诱导防御反应的量影响哈丁的组成型表达。 harpin是由与SEQ ID NO：2的氨基酸序列至少90％同源的氨基酸序列组成的蛋白质，并且具有过敏反应诱导活性。本发明还提供了用于生产转基因植物，表达盒，重组载体和编码竖琴的基因的方法。

7. 发明申请

US20090229011A1 DISEASE-RESISTANT PLANTS AND METHOD OF CONSTRUCTING THE SAME 失效
标题翻译：抗病植物及其构建方法
公开(公告)号：US20090229011A1
公开(公告)日：2009-09-10
申请号：US12406502
申请日：2009-03-18
申请人： Yoshimitsu TAKAKURA , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
发明人： Yoshimitsu TAKAKURA , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
IPC分类号： A01H5/00 , C12N15/82 , C12N15/63 , C07H21/04
CPC分类号： C12N15/8282 , C07K14/21
摘要： It is the object of the present invention to provide disease-resistant plants which have been transformed to cause an effective defense reaction, and methods for producing the same. The present invention provides expression cassettes comprising a promoter capable of promoting a constitutive, inducible, or organ- or phase-specific gene expression, and a gene, under the control of said promoter, encoding an elicitor protein.
摘要翻译：本发明的目的是提供已经转化以产生有效防御反应的抗病植物及其制备方法。本发明提供了包含能够促进组成型，诱导型或器官或相位特异性基因表达的启动子的表达盒，以及在所述启动子控制下编码引发蛋白的基因。

8. 发明授权

US06881720B1 Antimicrobial protein from Lyophyllum shimeji 失效
标题翻译：来自Lyophyllum shimeji的抗菌蛋白
公开(公告)号：US06881720B1
公开(公告)日：2005-04-19
申请号：US09856327
申请日：2000-09-20
申请人： Yoshimitsu Takakura , Shigeru Kuwata , Yasuhiro Inoue
发明人： Yoshimitsu Takakura , Shigeru Kuwata , Yasuhiro Inoue
IPC分类号： A01N37/46 , C07K14/375 , C12N1/21 , C12N15/31 , C12Q1/68 , A61K38/00
CPC分类号： C07K14/375 , A01N63/04 , C12Q1/689
摘要： The object of the present invention is to screen and identify a novel antimicrobial protein which can inhibit the growth of plant pathogenic microorganisms at a relatively low concentration such as Pyricularia oryzae and Rhizoctonia solani causative of two major diseases causing damage to rice crops and, further, to clone the gene of this protein. According to the present invention, an antimicrobial protein which can be obtained from a fraction of an aqueous extract of Lyophyllum shimeji precipitated by the ammonium sulfate precipitation method, has an antimicrobial activity at least against Rhizoctonia solani or Pyricularia oryzae, and shows the presence of components of about 70 kDa and/or about 65 kDa in molecular weight in the SDS-PAGE method. A gene encoding this protein and a method of using the same are provided.
摘要翻译：本发明的目的是筛选和鉴定一种新的抗微生物蛋白质，其可以以相对低的浓度抑制植物致病微生物的生长，例如造成稻谷作物损害的两种主要疾病的稻瘟病菌和立枯丝核菌属，克隆该蛋白质的基因。根据本发明，可以通过硫酸铵沉淀法沉淀出的一部分水杨酸提取物获得的抗微生物蛋白质具有抗菌活性，至少针对立枯丝核菌（Rhizoctonia solani）或Pyricularia oryzae，并显示了组分在SDS-PAGE方法中分子量约70kDa和/或约65kDa。提供了编码该蛋白质的基因及其使用方法。

9. 发明申请

US20110046352A1 DISEASE-RESISTANT PLANTS AND METHOD OF CONSTRUCTING THE SAME 审中-公开
标题翻译：抗病植物及其构建方法
公开(公告)号：US20110046352A1
公开(公告)日：2011-02-24
申请号：US12938645
申请日：2010-11-03
申请人： Yoshimitsu TAKAKURA , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
发明人： Yoshimitsu TAKAKURA , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
IPC分类号： C07K14/21
CPC分类号： C12N15/8282 , C07K14/21
摘要： The present invention provides transgenic, disease-resistant plants which have been transformed with an expression cassette including a constitutive gene expression promoter; and a gene, under the control of the promoter, encoding a harpin. The transformed cells in the transgenic plant effect the constitutive expression of the harpin in an amount effective for inducing a defense reaction. The harpin is a protein consisting of an amino acid sequence that is at least 90% homologous to the amino acid sequence of SEQ ID NO: 2, and possesses a hypersensitive-response-inducing activity. The present invention also provides methods for producing the transgenic plants, expression cassettes, recombinant vectors and genes encoding harpins.
摘要翻译：本发明提供已经用包含组成型基因表达启动子的表达盒转化的转基因抗病植物; 和在启动子控制下编码harpin的基因。转基因植物中的转化细胞以有效诱导防御反应的量影响哈丁的组成型表达。 harpin是由与SEQ ID NO：2的氨基酸序列至少90％同源的氨基酸序列组成的蛋白质，并且具有过敏反应诱导活性。本发明还提供了用于生产转基因植物，表达盒，重组载体和编码竖琴的基因的方法。

10. 发明申请

US20050288224A1 Novel protein, a gene coding therefor and a method of using the same 失效
标题翻译：新型蛋白质，其编码基因及其使用方法
公开(公告)号：US20050288224A1
公开(公告)日：2005-12-29
申请号：US11033116
申请日：2005-01-12
申请人： Yoshimitsu Takakura , Shigeru Kuwata , Yasuhiro Inoue
发明人： Yoshimitsu Takakura , Shigeru Kuwata , Yasuhiro Inoue
IPC分类号： A01N37/46 , C07K14/375 , C12N1/21 , C12N15/31 , C12Q1/68 , A61K38/17 , C07K14/195
CPC分类号： C07K14/375 , A01N63/04 , C12Q1/689
摘要： The object of the present invention is to screen and identify a novel antimicrobial protein which can inhibit the growth of plant pathogenic microorganisms at a relatively low concentration such as Pyricularia oryzae and Rhizoctonia solani causative of two major diseases causing damage to rice crops and, further, to clone the gene of this protein. According to the present invention, an antimicrobial protein which can be obtained from a fraction of an aqueous extract of Lyophyllum shimeji precipitated by the ammonium sulfate precipitation method, has an antimicrobial activity at least against Rhizoctonia solani or Pyricularia oryzae, and shows the presence of components of about 70 kDa and/or about 65 kDa in molecular weight in the SDS-PAGE method. A gene encoding this protein and a method of using the same are provided.
摘要翻译：本发明的目的是筛选和鉴定一种新的抗微生物蛋白质，其可以以相对低的浓度抑制植物致病微生物的生长，例如造成稻谷作物损害的两种主要疾病的稻瘟病菌和立枯丝核菌属，克隆该蛋白质的基因。根据本发明，可以通过硫酸铵沉淀法沉淀出的一部分水杨酸提取物获得的抗微生物蛋白质具有抗菌活性，至少针对立枯丝核菌（Rhizoctonia solani）或Pyricularia oryzae，并显示了组分在SDS-PAGE方法中分子量约70kDa和/或约65kDa。提供了编码该蛋白质的基因及其使用方法。

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