专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07525014B2 Disease-resistant plants and method of constructing the same 失效
标题翻译：抗病植物及其构建方法
公开(公告)号：US07525014B2
公开(公告)日：2009-04-28
申请号：US10363832
申请日：2001-09-07
申请人： Yoshimitsu Takakura , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
发明人： Yoshimitsu Takakura , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
IPC分类号： C12N15/09 , C12N15/31 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8282 , C07K14/21
摘要： It is the object of the present invention to provide disease-resistant plants which have been transformed to cause an effective defense reaction, and methods for producing the same.The present invention provides expression cassettes comprising a promoter capable of promoting a constitutive, inducible, or organ- or phase-specific gene expression, and a gene, under the control of said promoter, encoding an elicitor protein.
摘要翻译：本发明的目的是提供已经转化以产生有效防御反应的抗病植物及其制备方法。本发明提供了包含能够促进组成型，诱导型或器官或相位特异性基因表达的启动子的表达盒，以及在所述启动子控制下编码引发蛋白的基因。

2. 发明申请

US20110046352A1 DISEASE-RESISTANT PLANTS AND METHOD OF CONSTRUCTING THE SAME 审中-公开
标题翻译：抗病植物及其构建方法
公开(公告)号：US20110046352A1
公开(公告)日：2011-02-24
申请号：US12938645
申请日：2010-11-03
申请人： Yoshimitsu TAKAKURA , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
发明人： Yoshimitsu TAKAKURA , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
IPC分类号： C07K14/21
CPC分类号： C12N15/8282 , C07K14/21
摘要： The present invention provides transgenic, disease-resistant plants which have been transformed with an expression cassette including a constitutive gene expression promoter; and a gene, under the control of the promoter, encoding a harpin. The transformed cells in the transgenic plant effect the constitutive expression of the harpin in an amount effective for inducing a defense reaction. The harpin is a protein consisting of an amino acid sequence that is at least 90% homologous to the amino acid sequence of SEQ ID NO: 2, and possesses a hypersensitive-response-inducing activity. The present invention also provides methods for producing the transgenic plants, expression cassettes, recombinant vectors and genes encoding harpins.
摘要翻译：本发明提供已经用包含组成型基因表达启动子的表达盒转化的转基因抗病植物; 和在启动子控制下编码harpin的基因。转基因植物中的转化细胞以有效诱导防御反应的量影响哈丁的组成型表达。 harpin是由与SEQ ID NO：2的氨基酸序列至少90％同源的氨基酸序列组成的蛋白质，并且具有过敏反应诱导活性。本发明还提供了用于生产转基因植物，表达盒，重组载体和编码竖琴的基因的方法。

3. 发明授权

US07851671B2 Disease-resistant plants and method of constructing the same 失效
标题翻译：抗病植物及其构建方法
公开(公告)号：US07851671B2
公开(公告)日：2010-12-14
申请号：US12406502
申请日：2009-03-18
申请人： Yoshimitsu Takakura , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
发明人： Yoshimitsu Takakura , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
IPC分类号： A01H5/00 , C12N15/09 , C12N15/82 , C12N15/31
CPC分类号： C12N15/8282 , C07K14/21
摘要： The present invention provides transgenic, disease-resistant plants which have been transformed with an expression cassette including a constitutive gene expression promoter; and a gene, under the control of the promoter, encoding a harpin. The transformed cells in the transgenic plant effect the constitutive expression of the harpin in an amount effective for inducing a defense reaction. The harpin is a protein consisting of an amino acid sequence that is at least 90% homologous to the amino acid sequence of SEQ ID NO: 2, and possesses a hypersensitive-response-inducing activity. The present invention also provides methods for producing the transgenic plants, expression cassettes, recombinant vectors and genes encoding harpins.
摘要翻译：本发明提供已经用包含组成型基因表达启动子的表达盒转化的转基因抗病植物; 和在启动子控制下编码harpin的基因。转基因植物中的转化细胞以有效诱导防御反应的量影响哈丁的组成型表达。 harpin是由与SEQ ID NO：2的氨基酸序列至少90％同源的氨基酸序列组成的蛋白质，并且具有过敏反应诱导活性。本发明还提供了用于生产转基因植物，表达盒，重组载体和编码竖琴的基因的方法。

4. 发明申请

US20090229011A1 DISEASE-RESISTANT PLANTS AND METHOD OF CONSTRUCTING THE SAME 失效
标题翻译：抗病植物及其构建方法
公开(公告)号：US20090229011A1
公开(公告)日：2009-09-10
申请号：US12406502
申请日：2009-03-18
申请人： Yoshimitsu TAKAKURA , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
发明人： Yoshimitsu TAKAKURA , Yasuhiro Inoue , Shigeru Kuwata , Fumiki Tsutsumi , Yuji Ishida
IPC分类号： A01H5/00 , C12N15/82 , C12N15/63 , C07H21/04
CPC分类号： C12N15/8282 , C07K14/21
摘要： It is the object of the present invention to provide disease-resistant plants which have been transformed to cause an effective defense reaction, and methods for producing the same. The present invention provides expression cassettes comprising a promoter capable of promoting a constitutive, inducible, or organ- or phase-specific gene expression, and a gene, under the control of said promoter, encoding an elicitor protein.
摘要翻译：本发明的目的是提供已经转化以产生有效防御反应的抗病植物及其制备方法。本发明提供了包含能够促进组成型，诱导型或器官或相位特异性基因表达的启动子的表达盒，以及在所述启动子控制下编码引发蛋白的基因。

5. 发明申请

US20100132068A1 Cosmid Vector For Plant Transformation And Use Thereof 有权
标题翻译：用于植物转化和使用的粘粒载体
公开(公告)号：US20100132068A1
公开(公告)日：2010-05-27
申请号：US12306163
申请日：2007-06-25
申请人： Yoshimitsu Takakura , Toshihiko Komari , Yuji Ishida , Toshiyuki Komori , Yukoh Hiei , Toshiki Mine , Teruyuki Imayama
发明人： Yoshimitsu Takakura , Toshihiko Komari , Yuji Ishida , Toshiyuki Komori , Yukoh Hiei , Toshiki Mine , Teruyuki Imayama
IPC分类号： A01H1/00 , C12N15/63
CPC分类号： C12N15/82 , C12N15/8205
摘要： The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium; 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium; 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium; 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.
摘要翻译：本发明旨在提供用于植物转化的新型载体。本发明的载体是全长为15kb或更小的粘粒载体，其特征在于：1）它们含有IncP质粒的复制起点，但不含有其他质粒组的任何复制起点; 2）它们含有IncP质粒的trfA1基因; 3）它们含有一个IncP质粒的oriT; 4）它们含有IncP质粒的incC1基因; 5）它们含有λ噬菌体的cos位点，cos位点位于T-DNA外部; 6）它们含有在大肠杆菌中表达的耐药基因和农杆菌属的细菌; 7）它们含有农杆菌属细菌的T-DNA右侧序列; 8）它们含有农杆菌属细菌的T-DNA左边界序列; 9）它们含有位于7）和8之间的植物转化的选择性标记基因并在植物中表达; 和10）它们含有位于7）和8之间的限制性内切核酸酶识别位点，用于克隆外来基因。

6. 发明授权

US08298819B2 Cosmid vector for plant transformation and use thereof 有权
标题翻译：用于植物转化的粘粒载体及其用途
公开(公告)号：US08298819B2
公开(公告)日：2012-10-30
申请号：US12306163
申请日：2007-06-25
申请人： Yoshimitsu Takakura , Toshihiko Komari , Yuji Ishida , Toshiyuki Komori , Yukoh Hiei , Toshiki Mine , Teruyuki Imayama
发明人： Yoshimitsu Takakura , Toshihiko Komari , Yuji Ishida , Toshiyuki Komori , Yukoh Hiei , Toshiki Mine , Teruyuki Imayama
IPC分类号： C12N15/82 , C12N15/19 , C12N15/00
CPC分类号： C12N15/82 , C12N15/8205
摘要： The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium; 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium; 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium; 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.
摘要翻译：本发明旨在提供用于植物转化的新型载体。本发明的载体是全长为15kb或更小的粘粒载体，其特征在于：1）它们含有IncP质粒的复制起点，但不含有其他质粒组的任何复制起点; 2）它们含有IncP质粒的trfA1基因; 3）它们含有一个IncP质粒的oriT; 4）它们含有IncP质粒的incC1基因; 5）它们含有λ噬菌体的cos位点，cos位点位于T-DNA外部; 6）它们含有在大肠杆菌中表达的耐药基因和农杆菌属的细菌; 7）它们含有农杆菌属细菌的T-DNA右侧序列; 8）它们含有农杆菌属细菌的T-DNA左边界序列; 9）它们含有位于7）和8之间的植物转化的选择性标记基因并在植物中表达; 和10）它们含有位于7）和8之间的限制性内切核酸酶识别位点，用于克隆外来基因。

7. 发明授权

US08039687B2 Method for screening genomic DNA fragments 有权
标题翻译：筛选基因组DNA片段的方法
公开(公告)号：US08039687B2
公开(公告)日：2011-10-18
申请号：US10576693
申请日：2004-10-22
申请人： Tomoaki Kubo , Toshihiko Komari , Satoru Usami , Yoshimitsu Takakura , Yukoh Hiei , Yuji Ishida
发明人： Tomoaki Kubo , Toshihiko Komari , Satoru Usami , Yoshimitsu Takakura , Yukoh Hiei , Yuji Ishida
IPC分类号： C12N15/82 , C12N15/10 , C12N15/29 , C12N15/55 , C12N15/70 , C12N15/84
CPC分类号： A01H1/00 , A01H1/04 , C12N15/8201 , C12N15/8261 , Y02A40/146
摘要： The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement. The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.
摘要翻译：本发明提供了一种选择可用于提供具有农业上有利改进的植物的基因组DNA片段的方法。本发明的方法包括以下步骤：1）从植物制备基因组DNA，然后将其克隆到克隆载体中以形成基因组DNA文库; 2）将构成基因组DNA文库的每个基因组克隆的基因组片段分别引入植物中以产生转基因植物; 3）培育转基因植物或其后代以选择表现出农业上有利的表型变异的植物; 和4）将在步骤（2）中引入的基因组DNA片段选择在步骤（3）中选择的植物中，作为目的基因组DNA片段。

8. 发明申请

US20080301832A1 Method for Screening Genomic Dna Fragments 有权
标题翻译：筛选基因组DNA片段的方法
公开(公告)号：US20080301832A1
公开(公告)日：2008-12-04
申请号：US10576693
申请日：2004-10-22
申请人： Tomoaki Kubo , Toshihiko Komari , Satoru Usami , Yoshimitsu Takakura , Yukoh Hiei , Yuji Ishida
发明人： Tomoaki Kubo , Toshihiko Komari , Satoru Usami , Yoshimitsu Takakura , Yukoh Hiei , Yuji Ishida
IPC分类号： C12N15/11 , C12Q1/68 , A01H5/00 , C12P19/28
CPC分类号： A01H1/00 , A01H1/04 , C12N15/8201 , C12N15/8261 , Y02A40/146
摘要： The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement.The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.
摘要翻译：本发明提供了一种选择可用于提供具有农业上有利改进的植物的基因组DNA片段的方法。本发明的方法包括以下步骤：1）从植物制备基因组DNA，然后将其克隆到克隆载体中以形成基因组DNA文库; 2）将构成基因组DNA文库的每个基因组克隆的基因组片段分别引入植物中以产生转基因植物; 3）培育转基因植物或其后代以选择表现出农业上有利的表型变异的植物; 和4）将在步骤（2）中引入的基因组DNA片段选择在步骤（3）中选择的植物中，作为目的基因组DNA片段。

9. 发明授权

US09284567B2 Method for gene introduction into hordeum plant using agrobacterium, and method for production of transformed plant of hordeum plant 有权
标题翻译：使用农杆菌将基因引入大麦植物的方法，以及用于生产大麦植物转化植物的方法
公开(公告)号：US09284567B2
公开(公告)日：2016-03-15
申请号：US13812412
申请日：2011-07-29
申请人： Yukoh Hiei , Yuji Ishida
发明人： Yukoh Hiei , Yuji Ishida
IPC分类号： A01H4/00 , C12N15/90 , C12N15/82
CPC分类号： C12N15/8205 , A01H4/008
摘要： An object of the present invention is to provide a method of gene introduction, which can transform a Hordeum plant at a higher efficiency compared to that in known Agrobacterium methods, and a method of producing a transformed Hordeum plant. The method of the invention includes a step of subjecting an immature embryo tissue of a Hordeum plant to centrifugation treatment and/or pressurization treatment before the inoculation with Agrobacterium, during the coculture step, and/or after the coculture step, and is characterized in that the coculture medium satisfies at least one of a) containing an antiauxin; b) containing a cytokinin; and c) containing a phenoxy auxin in an amount of less than 2 μM and/or a benzoic auxin in an amount of less than 5 μM, or not containing any phenoxy auxin and/or benzoic auxin.
摘要翻译：本发明的目的是提供一种基因导入方法，其可以与已知的农杆菌方法相比以更高的效率转化大麦植物，以及生产转化的大麦草植物的方法。本发明的方法包括在共培养步骤期间和/或共培养步骤之后，在土壤杆菌接种之前和/或共培养步骤之后，使大麦植物的未成熟胚胎组织进行离心处理和/或加压处理的步骤，其特征在于共培养培养基满足以下中的至少一种：a）含有抗癌素; b）含有细胞分裂素; 和c）含有少于2μM的苯氧基生长素和/或小于5μM的苯甲酸生长素，或不含任何苯氧基生长素和/或苯甲酸生长素。

10. 发明授权

US08193552B2 Semiconductor light emitting device of junction-down type and semiconductor light emitting element of junction-down type 有权
标题翻译：结合型半导体发光元件和结合型半导体发光元件
公开(公告)号：US08193552B2
公开(公告)日：2012-06-05
申请号：US12320841
申请日：2009-02-05
申请人： Yuji Ishida
发明人： Yuji Ishida
IPC分类号： H01L33/00
CPC分类号： H01S5/34333 , B82Y20/00 , H01L2224/16225 , H01S5/0224 , H01S5/02272 , H01S5/02276 , H01S5/22
摘要： In a semiconductor light emitting device of junction-down type, a semiconductor light emitting element having a stripe part is provided with a bonding part for die bonding in a part of a surface thereof where the stripe part is formed, the bonding part being at a position away from the stripe part, and being junction-down bonded onto an electrode pattern on a holding substrate.
摘要翻译：在结低型半导体发光器件中，具有条纹部分的半导体发光元件在其形成条纹部分的表面的一部分中设置有用于芯片接合的接合部分，所述接合部分处于位置远离条纹部分，并且被下降结合到保持基板上的电极图案上。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式