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    • 3. 发明授权
    • Method and apparatus for continuous heat sterilization of liquid
    • 液体连续加热灭菌的方法和装置
    • US06673311B1
    • 2004-01-06
    • US09529956
    • 2000-06-26
    • Kazuyoshi SotoyamaKeiji IwatsukiTeruhiko MizotaYuzo AsanoYasutatsu MizotaHiroaki MatsuiTetsushi Mori
    • Kazuyoshi SotoyamaKeiji IwatsukiTeruhiko MizotaYuzo AsanoYasutatsu MizotaHiroaki MatsuiTetsushi Mori
    • A61L200
    • A23L3/0155A23L3/16A23L3/18A23L3/22A61L2/04
    • A method for continuous heat sterilization of liquid includes a continuous pre-heating step of preheating the liquid, a final heating step of heating the preheated liquid to reach a predetermined sterilizing temperature, a holding step of holding the liquid which has reached the predetermined sterilizing temperature for a predetermined period of time, and a cooling step of cooling the held liquid. The final heating step comprises the following steps (a) and (b): (a) a step of continuously pressurizing the liquid by a high pressure pump; and (b) a step of releasing the liquid continuously to a normal pressure within a time period of less than 10 seconds after the pressurizing step to thereby make the liquid reach the predetermined sterilizing temperature. An apparatus for automatically carrying out the method is provided. According to the continuous heat sterilization method and apparatus, scorch during the final heating step is dispensed with and accordingly, a long period of continuous operation is feasible. Furthermore, denaturation of liquid caused by heating is inconsiderable, investment cost and running cost become inexpensive, and a large amount of liquid can be processed.
    • 一种液体的连续加热灭菌方法,包括对液体进行预热的连续预热工序,对预热液进行加热达到规定的灭菌温度的最终加热工序,保持达预定灭菌温度的液体的保持步骤 预定时间段,以及冷却保持液体的冷却步骤。 最终加热步骤包括以下步骤(a)和(b):(a)通过高压泵连续加压液体的步骤; 和(b)在加压步骤后的10秒以内的时间内将液体连续地释放到常压的步骤,使液体达到规定的灭菌温度。 提供一种用于自动执行该方法的装置。 根据连续加热灭菌方法和装置,省去了最终加热步骤期间的焦烧,因此长时间的连续操作是可行的。 此外,由加热引起的液体的变性是不可忽视的,因此投资成本和运行成本便宜,并且可以处理大量的液体。
    • 9. 发明授权
    • Process for the preparation of instant agar medium
    • 制备速溶琼脂培养基的方法
    • US06337210B1
    • 2002-01-08
    • US09530945
    • 2000-06-06
    • Mamoru TomitaKazuyoshi SotoyamaTutomu KudoKenji MizuguchiKenji KiyotakiDaiki Tanno
    • Mamoru TomitaKazuyoshi SotoyamaTutomu KudoKenji MizuguchiKenji KiyotakiDaiki Tanno
    • C12N500
    • C12N1/20
    • A process for the preparation of an instant agar medium which can be immediately used as a culture medium merely by heating a container packed with the agar medium just before the use for a short time, can be stored for a long period and little suffers from the deterioration of medium components. Specifically, a process for the preparation of an instant agar medium which can be dissolved by heating for a short time just before the use, characterized by dissolving under heating 10 to 80 wt. % of a prescribed amount of agar to be used in an agar medium in part of a prescribed amount of water to be used therein, cooling the obtained solution, separately dispersing or dissolving the rest of agar and the other culture medium components in the rest of water, mixing the cooled solution with the separately prepared dispersion or solution, adjusting the viscosity of the obtained fluid to 40 to 4,000 mPa·s, packing the resulting fluid into a container, hermetically sealing the container, and sterilizing it.
    • 用于制备即用琼脂培养基的方法,其可以仅在短时间内使用之前加热装有琼脂培养基的容器即可立即用作培养基,可以长时间储存​​,并且很少受到 介质成分的劣化。 具体而言,是一种即时琼脂培养基的制备方法,其可以在使用前短时间加热溶解,其特征在于,在加热下溶解10〜80重量% 在琼脂培养基中使用的规定量的琼脂的一定量的一定量的一部分用于其中的规定量的水,冷却所得溶液,将其余琼脂和其它培养基成分分散或溶解在其余部分中 将冷却的溶液与单独制备的分散体或溶液混合,将获得的流体的粘度调节至40至4000mPa.s,将所得流体包装到容器中,气密地密封容器并对其进行消毒。