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1. 发明授权

US5066462A Analytical element for measuring enzyme activity 失效
标题翻译：测定酶活性的分析元素
公开(公告)号：US5066462A
公开(公告)日：1991-11-19
申请号：US401494
申请日：1989-08-29
申请人： Kazuya Kawasaki , Mitsutoshi Tanaka , Yoshikazu Amano , Harumi Katsuyama
发明人： Kazuya Kawasaki , Mitsutoshi Tanaka , Yoshikazu Amano , Harumi Katsuyama
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/26 , C12Q1/48 , C12Q1/52 , G01N33/52
CPC分类号： C12Q1/48 , G01N33/525 , Y10S435/805
摘要： A dry-type analytical element suitable for measuring the activity of alanine aminotransferase in a liquid sample, characterized by incorporating a dye capable of absorbing the electromagnetic waves of 400 to 500 nm into at least one water-permeable layer. The coloring sensitivity of the dry-type analytical element does not increase substantially even under a fluorescent light, and thereby, an accurate measured value can easily be obtained.
摘要翻译：适用于测量液体样品中丙氨酸氨基转移酶活性的干型分析元件，其特征在于将能够吸收400-500nm电磁波的染料掺入到至少一个透水层中。即使在荧光下，干式分析元件的着色灵敏度也不会显着增加，从而可以容易地获得准确的测量值。

2. 发明授权

US5508173A Analytical element for measurements of enzyme activity 失效
标题翻译：酶活性测定用分析元素
公开(公告)号：US5508173A
公开(公告)日：1996-04-16
申请号：US373885
申请日：1995-01-17
申请人： Yoshikazu Amano , Kazuya Kawasaki , Harumi Katsuyama
发明人： Yoshikazu Amano , Kazuya Kawasaki , Harumi Katsuyama
IPC分类号： G01N31/22 , C12Q1/00 , G01N33/52 , C12Q1/28 , C12Q1/26 , C12Q1/52
CPC分类号： G01N33/525 , G01N33/526
摘要： A multilayer analytical element for measuring enzyme activity in a liquid sample such as a body fluid which is less affected by intrinsic pyruvic acid contained in the liquid sample is disclosed. The analytical element comprises a support, a reagent layer, an optionally provided other layer, and a porous spreading layer. The reagent layer and/or the optionally provided other layer contain pyruvic acid oxidase. The characteristic feature of the analytical element lies in that the spreading layer has a specific void volume in the range of 3-15 .mu.l/cm.sup.2.
摘要翻译：公开了一种用于测量液体样品中的酶活性的多层分析元件，所述液体样品例如体液中较少受到液体样品中所含的固有丙酮酸的影响。分析元件包括载体，试剂层，任选提供的其它层和多孔扩散层。试剂层和/或任选提供的其它层含有丙酮酸氧化酶。分析元件的特征在于扩散层具有在3-15μml/ cm 2范围内的特定空隙体积。

3. 依法登记的发明

USH600H Whole blood analytical element and method of analyzing whole blood using the same 失效
标题翻译：全血分析元素及全血分析方法
公开(公告)号：USH600H
公开(公告)日：1989-03-07
申请号：US171326
申请日：1988-03-21
申请人： Mitzutoshi Tanaka , Shigeru Nagatomo , Harumi Katsuyama , Kazuya Kawasaki
发明人： Mitzutoshi Tanaka , Shigeru Nagatomo , Harumi Katsuyama , Kazuya Kawasaki
IPC分类号： G01N33/52
CPC分类号： G01N33/525
摘要： A multilayer analytical element for determining an assay of a component in whole blood sample is disclosed, which comprises at least two water-permeable layers, wherein one layer is a sample spreading layer and the other layer is a reagent layer, wherein one layer or more of said water-permeable layers contain a composition which interacts with said component in whole blood sample, wherein the composition forms a dye capable of being detected by a spectrophotometry at a wavelength of 600 n.m. or longer, when the composition interacts with said component in whole blood sample.

4. 依法登记的发明

USH622H Analytical element for measuring activity of creatine kinase 失效
标题翻译：用于测量肌酸激酶活性的分析元素
公开(公告)号：USH622H
公开(公告)日：1989-04-04
申请号：US195238
申请日：1988-05-18
申请人： Shigeki Kageyama , Kazuya Kawasaki , Harumi Katsuyama
发明人： Shigeki Kageyama , Kazuya Kawasaki , Harumi Katsuyama
IPC分类号： C12Q1/50
CPC分类号： C12Q1/50
摘要： In a dry analytical element for the determination of creatine kinase enzymatic activity is disclosed, comprising at least one porous medium and at least one water permeable layer having a liquid contact relation with the porous medium, the porous medium or the water permeable layer contains creatine phosphoric acid, ADP, a thiol compound as a creatine kinase activation agent, glucose, hexokinase, G6PDH, NAD.sup.+, an electron transmission agent and a formazane dye forming tetrazolium salt, the improvement wherein at least one water permeable layer having a liquid contact relation with the porous medium contains a formazane dye forming tetrazolium salt and not more than 16 g/m.sup.2 of gelatin.

5. 发明授权

US5496708A Reagent composition for quantitative analysis of inorganic phosphorous and dry analysis element utilizing the same 失效
标题翻译：用于定量分析无机磷和干分析元素的试剂组合物
公开(公告)号：US5496708A
公开(公告)日：1996-03-05
申请号：US259616
申请日：1994-06-14
申请人： Kazuya Kawasaki , Yoshikazu Amano , Osamu Seshimoto , Kiyoshi Yamada
发明人： Kazuya Kawasaki , Yoshikazu Amano , Osamu Seshimoto , Kiyoshi Yamada
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/48 , G01N31/22
CPC分类号： C12Q1/48 , Y10S435/805 , Y10S435/97
摘要： A reagent composition for the quantitative analysis of inorganic phosphorus, composted of xanthosine, purine nucleoside phosphorylase, xanthine oxidase and a color-former. The color-former is a precursor for forming a dye in the presence of hydrogen peroxide and peroxidase. The reagent composition of the invention is improved in storage stability and has a wider determination range. Also provided is a dry analysis element composed of reagent layer containing the inventive reagent composition.
摘要翻译：用于定量分析无机磷的试剂组合物，堆肥的黄嘌呤，嘌呤核苷磷酸化酶，黄嘌呤氧化酶和成色剂。成色剂是在过氧化氢和过氧化物酶存在下形成染料的前体。本发明的试剂组合物的贮存稳定性提高，判定范围更广。还提供了由包含本发明试剂组合物的试剂层组成的干分析元件。

6. 发明授权

US07105128B2 Dry analytical element using water-soluble indicator for colorimetry 有权
公开(公告)号：US07105128B2
公开(公告)日：2006-09-12
申请号：US10281564
申请日：2002-10-28
申请人： Hideaki Tanaka , Yoshihide Iwaki , Yoshiki Sakaino , Kazuya Kawasaki , Yoshikazu Amano
发明人： Hideaki Tanaka , Yoshihide Iwaki , Yoshiki Sakaino , Kazuya Kawasaki , Yoshikazu Amano
IPC分类号： G01N31/22
CPC分类号： G01N33/525 , G01N33/84
摘要： This invention relates to a dry analytical element using a water-soluble indicator which improves measuring accuracy, which comprises a water-impermeable support, a water-permeable layer and a spreading layer having a function to spread a liquid uniformly, wherein a water-soluble indicator for colorimetry is incorporated into the s preading layer.

7. 发明申请

US20050106757A1 Agglutination assay method in porous medium layer 审中-公开
标题翻译：多孔介质层凝集测定法
公开(公告)号：US20050106757A1
公开(公告)日：2005-05-19
申请号：US11022510
申请日：2004-12-22
申请人： Kentaro Nakamura , Kazuya Kawasaki , Osamu Seshimoto , Masahito Nagata , Toru Tanaka
发明人： Kentaro Nakamura , Kazuya Kawasaki , Osamu Seshimoto , Masahito Nagata , Toru Tanaka
IPC分类号： G01N33/52 , G01N33/543 , G01N33/58 , G01N33/551
CPC分类号： G01N33/54346 , G01N33/525 , G01N33/587
摘要： An agglutination assay method for quantitatively determination of an analyte in an aqueous liquid sample using particles bearing an anti-analyte. The agglutination is conducted in the porous medium layer of the analysis element. A speedy quantitative analysis of the analyte can be conveniently attained with high sensitivity. When the particle-labeled anti-analyte is contained in the porous medium layer, the anti-analyte can be stored with higher stability in the dry state. A dry analysis element for enabling such analysis method is also provided.
摘要翻译：一种用于使用带有抗分析物的颗粒定量测定含水液体样品中的分析物的凝集测定方法。凝集在分析元件的多孔介质层中进行。可以方便地以高灵敏度获得分析物的快速定量分析。当颗粒标记的抗分析物包含在多孔介质层中时，抗分析物可以在干燥状态下以更高的稳定性储存。还提供了用于实现这种分析方法的干燥分析元件。

8. 发明申请

US20080019869A1 Multilayer Analysis Element 审中-公开
标题翻译：多层分析单元
公开(公告)号：US20080019869A1
公开(公告)日：2008-01-24
申请号：US10589556
申请日：2005-02-18
申请人： Kentaro Nakamura , Kazuya Kawasaki , Yoshiki Sakaino , Toshihisa Ito , Osamu Seshimoto
发明人： Kentaro Nakamura , Kazuya Kawasaki , Yoshiki Sakaino , Toshihisa Ito , Osamu Seshimoto
IPC分类号： B01L11/00
CPC分类号： G01N33/525
摘要： It is an object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for liquid sample analysis wherein at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in this order on one side of a water-impermeable planar support, and the non-fibrous porous film has a bending rupture strength of 20 gram-weight or more and a tensile percentage of 2% or less when the film is stretched with a tensile force of 50 gram-weight.
摘要翻译：本发明的目的是提供一种具有很小的批量间和批间差异的多层分析元件，其具有高的测量精度并且尺寸小。本发明提供了一种用于液体样品分析的多层分析元件，其中至少一个功能层和非纤维多孔膜的至少一个多孔液体样品扩散层在不透水平面支撑体的一侧上依次一体地层叠，并且当以50克重的拉伸力拉伸膜时，非纤维状多孔膜的弯曲断裂强度为20克重以上，拉伸率为2％以下。

9. 发明授权

US08906641B2 Method for measuring animal α-amylase 有权
标题翻译：测定动物α-淀粉酶的方法
公开(公告)号：US08906641B2
公开(公告)日：2014-12-09
申请号：US12015151
申请日：2008-01-16
申请人： Shigeki Kageyama , Nobuhito Masuda , Kazuya Kawasaki , Satoru Toda
发明人： Shigeki Kageyama , Nobuhito Masuda , Kazuya Kawasaki , Satoru Toda
IPC分类号： C12Q1/40 , G01N33/52
CPC分类号： C12Q1/40 , G01N33/523 , G01N33/526
摘要： It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring α-amylase even in a sample comprising animal α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring α-amylase contained in a non-human animal sample comprising α-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7.
摘要翻译：本发明的目的是提供一种能够测量高浓度（高达2500U / L）的动物样品而不稀释的方法，即使在包含动物α-淀粉样品的样品中也能够特异性地测量α-淀粉酶，淀粉酶（EC 3.2.1.1）和葡糖淀粉酶（EC 3.2.1.3）。本发明提供一种用于特异性测量包含α-淀粉酶和葡糖淀粉酶的非人动物样品中α-淀粉酶的方法，而不稀释所述样品，其中通过使用具有受保护的非还原末端的寡糖和对硝基苯在其还原末端，反应的pH为6以上且小于7。

10. 发明申请

US20050186110A1 Multilayer analysis element 审中-公开
标题翻译：多层分析单元
公开(公告)号：US20050186110A1
公开(公告)日：2005-08-25
申请号：US11060777
申请日：2005-02-18
申请人： Kentaro Nakamura , Kazuya Kawasaki , Toshihisa Ito , Yoshiki Sakaino , Osamu Seshimoto
发明人： Kentaro Nakamura , Kazuya Kawasaki , Toshihisa Ito , Yoshiki Sakaino , Osamu Seshimoto
IPC分类号： G01N31/22
CPC分类号： G01N31/22
摘要： It is an object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for the analysis of a liquid sample, comprising a water impermeable planar support on one side of which at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in the mentioned order, wherein said spreading layer comprises a non-fibrous porous film such that, when a liquid sample containing a target substance to be measured is caused to become attached to the surface of said liquid sample spreading layer, where said liquid sample is spread and dispersed, 30% or more of said target substance to be measured can be transferred to the at least one functional layer below said liquid sample spreading layer.
摘要翻译：本发明的目的是提供一种具有很小的批量间和批间差异的多层分析元件，其具有高的测量精度并且尺寸小。本发明提供了一种用于分析液体样品的多层分析元件，其包括不透水平面支撑体，其一侧上至少一个功能层和至少一个非纤维多孔膜的多孔液体样品铺展层整体层压其中所述扩散层包括非纤维多孔膜，使得当将含有待测物体的液体样品附着在所述液体样品扩散层的表面上时，其中所述液体样品为扩散和分散，所述待测量的所述目标物质的30％以上可以转移到所述液体样品扩散层下方的至少一个功能层。

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