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1. 发明授权

US6153419A Method for quantitative determination of 1,5-anhydroglucitol 失效
标题翻译： 1,5-脱水葡萄糖醇定量测定方法
公开(公告)号：US6153419A
公开(公告)日：2000-11-28
申请号：US930709
申请日：1997-10-16
申请人： Kazuo Aisaka , Sakae Tazoe , Katsuhiko Ando , Keiko Ochiai
发明人： Kazuo Aisaka , Sakae Tazoe , Katsuhiko Ando , Keiko Ochiai
IPC分类号： C12N9/24 , C12Q1/34 , C12Q1/48
CPC分类号： C12Q1/34 , C12N9/2402 , C12Q1/48 , C12Y302/01028 , G01N2333/355 , G01N2333/924 , Y10S435/872
摘要： The present invention relates to a method for quantitative determination of 1,5-anhydroglucitol in a sample, which comprises mixing the sample and an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, and measuring the activity of the enzyme; and a reagent for quantitative determination of 1,5-anhydroglucitol which comprises an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, a substrate for the enzyme, and a reagent for quantitative determination of a product formed by the enzyme activity. The present invention also relates to novel trehalase having a Ki value of 0.33 mM or less for 1,5-anhydroglucitol; and a process for producing novel trehalase having the above-mentioned physicochemical properties, which comprises culturing in a medium a microorganism belonging to the genus Nocardia and capable of producing the trehalase, and recovering the trehalase from the culture.
摘要翻译： PCT No.PCT / JP97 / 00440 Sec。 371日期：1997年10月16日 102（e）1997年10月16日PCT PCT 1997年2月19日PCT公布。公开号WO97 / 31103 日期：1997年8月28日本发明涉及一种样品中1,5-脱水葡萄糖醇的定量测定方法，该方法包括以浓度依赖性方式将样品与具有1,5-脱水葡萄糖醇抑制活性的酶，并测定酶的活性; 以及用于定量测定1,5-脱水葡萄糖醇的试剂，该试剂含有以浓度依赖性方式被1,5-脱水葡萄糖醇抑制的活性的酶，酶底物和定量产物形成试剂通过酶活性。本发明还涉及1,5-脱水葡萄糖醇的Ki值为0.33mM以下的新型海藻糖酶; 以及具有上述物理化学性质的新型海藻糖酶的制造方法，其包括在培养基中培养属于诺卡氏菌属的微生物并能够生产海藻糖酶，并从培养物中回收海藻糖酶的方法。

2. 发明授权

US06485931B2 CHOLESTEROL DEHYDROGENASE, COPROSTAN-3-ONE DEHYDROGENASE AND 4-CHOLESTEN-3-ONE DEHYDROGENASE, COMPOSITIONS CONTAINING THE DEHYDROGENASES, AND METHOD FOR REDUCING AMOUNT OF CHOLESTEROL USING THE COMPOSITIONS 失效
标题翻译：胆固醇脱氢酶，COPROSTAN-3-ONE脱氢酶和4-烯丙基-3-酮脱氢酶，含有脱氢酶的组合物，以及使用组合物降低胆固醇含量的方法
公开(公告)号：US06485931B2
公开(公告)日：2002-11-26
申请号：US09750692
申请日：2001-01-02
申请人： Chiaki Saitoh , Hideyo Kumazawa , Kazuo Aisaka , Toru Mizukami , Katsuhiko Ando , Keiko Ochiai , Ryoichi Katsumata
发明人： Chiaki Saitoh , Hideyo Kumazawa , Kazuo Aisaka , Toru Mizukami , Katsuhiko Ando , Keiko Ochiai , Ryoichi Katsumata
IPC分类号： C12P104
CPC分类号： C12Y101/99001 , A23K20/189 , A23L5/25 , A23L13/48 , A23L15/25 , A23L17/65 , C12N9/0006 , C12P33/00
摘要： According to the present invention, a process for producing a practical cholesterol-reduced substance by converting cholesterol contained in foods and feeds to coprostanol having very low intestinal tract absorbability by utilizing enzymatic action is provided. Cholesterol in a cholesterol-containing substance such as meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry and pisciculture, and the like, can be treated with three kinds of enzymes which are a cholesterol dehydrogenase having an optimum pH in a neutral pH range and 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase each having an optimum pH in a weak acidic range, or microbial cells containing the enzymes, for converting the cholesterol to coprostanol to reduce the amount of the cholesterol.
摘要翻译：根据本发明，提供了通过利用酶作用将食品和饲料中所含的胆固醇转化为具有非常低肠吸收能力的丙泊酚的生产实用性降胆固醇物质的方法。含胆固醇的物质如肉，鸡蛋，牛奶，海产品，含有该食品的熟化加工食品，动物，禽类和养殖用饲料等都可以用三种酶在中性pH范围内具有最佳pH值的胆固醇脱氢酶 - 胆固醇-3-酮脱氢酶和拮抗佐-3-酮脱氢酶，其各自具有弱酸性范围内的最佳pH，或含有酶的微生物细胞，用于将胆固醇转化为coprostanol以降低胆固醇的量。

3. 发明授权

US06312919B1 Process for producing a cholesterol-reduced substance 失效
标题翻译：降低胆固醇的物质的制造方法
公开(公告)号：US06312919B1
公开(公告)日：2001-11-06
申请号：US09101327
申请日：1998-07-07
申请人： Chiaki Saitoh , Hideyo Kumazawa , Kazuo Aisaka , Toru Mizukami , Katsuhiko Ando , Keiko Ochiai , Ryoichi Katsumata , Kozo Ouchi
发明人： Chiaki Saitoh , Hideyo Kumazawa , Kazuo Aisaka , Toru Mizukami , Katsuhiko Ando , Keiko Ochiai , Ryoichi Katsumata , Kozo Ouchi
IPC分类号： C12P3300
CPC分类号： C12Y101/99001 , A23K20/189 , A23L5/25 , A23L13/48 , A23L15/25 , A23L17/65 , C12N9/0006 , C12P33/00
摘要： An enzymatic process is presented for reducing the cholesterol level of foods and feeds by converting the cholesterol therein to coprostanol which has a very low intestinal tract absorbability. Cholesterol in meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry, and pisiculture can be reduced by the sequential action of three enzymes isolated from a eubacterium: cholesterol dehydrogenase, 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase.
摘要翻译：提出了一种酶法，用于通过将其中的胆固醇转化成具有非常低的肠道吸收性的coprostanol来降低食物和饲料的胆固醇水平。肉，蛋，牛奶，海鲜和含有此类食物的熟化加工食品中的胆固醇或动物，家禽和养殖用饲料可以通过从真菌分离的三种酶的顺序作用来降低：胆固醇脱氢酶，4-胆甾醇-3- 一种脱氢酶和副雄甾-3-酮脱氢酶。

4. 发明授权

US07037672B2 Process for producing a bran pickles flavoring solution 失效
公开(公告)号：US07037672B2
公开(公告)日：2006-05-02
申请号：US10419095
申请日：2003-04-21
申请人： Chiaki Saitoh , Atsushi Yashiro , Chikara Tokunaga , Kyoko Ozawa , Atsuko Yokoi , Nobuo Ogata , Hiroshi Katahira , Keiko Ochiai , Katsuhiko Ando
发明人： Chiaki Saitoh , Atsushi Yashiro , Chikara Tokunaga , Kyoko Ozawa , Atsuko Yokoi , Nobuo Ogata , Hiroshi Katahira , Keiko Ochiai , Katsuhiko Ando
IPC分类号： C12P1/00
CPC分类号： C12R1/15 , A23B7/10 , A23L27/24 , C12P17/04 , Y10S435/843
摘要： The present invention relates to a novel flavoring solution for making pickles having a flavor of foods pickled in rice bran paste (hereinafter referred to as a bran pickles flavoring solution), a clear bran pickles flavoring solution, processes for producing said solutions, a novel microorganism belonging to the genus Corynebacterium used for the production of said flavoring solutions, and a process for producing a solution containing a lactone wherein the lactone is selectively extracted from a culture of a lactone-producing microorganism.The present invention also relates to a novel bacterium of the genus Corynebacterium which has the ability to produce γ-dodecalactone and/or γ-dodecelactone and is useful for the production of said bran pickles flavoring solutions.

5. 发明授权

US06468780B2 Bacillus strains which produce HMG-CoA reductase inhibitors 有权
标题翻译：产生HMG-CoA还原酶抑制剂的芽孢杆菌菌株
公开(公告)号：US06468780B2
公开(公告)日：2002-10-22
申请号：US09829080
申请日：2001-04-10
申请人： Yutaka Takano , Masaru Hasegawa , Hideo Mori , Katsuhiko Ando , Keiko Ochiai , Hiroaki Motoyama , Akio Ozaki
发明人： Yutaka Takano , Masaru Hasegawa , Hideo Mori , Katsuhiko Ando , Keiko Ochiai , Hiroaki Motoyama , Akio Ozaki
IPC分类号： C12N120
CPC分类号： C12R1/07 , C12P7/62 , C12P17/06
摘要： Disclosed is a process for producing a compound represented by general formula (II-a): (wherein R1 represents a hydrogen atom, a substituted or unsubstituted alkyl group, or an alkali metal; and R2 represents a substituted or unsubstituted alkyl or aryl group) [hereinafter referred to as Compound (II-a)] or the lactone form of Compound (II-a) [hereinafter referred to as Compound (II-b)] which comprises subjecting a compound represented by general formula (I-a): (wherein R1 represents a hydrogen atom, a substituted or unsubstituted alkyl group, or an alkali metal; and R2 represents a substituted or unsubstituted alkyl or aryl group) [hereinafter referred to as Compound (I-a)] or the lactone form of Compound (I-a) [hereinafter referred to as Compound (I-b)] to the action of an enzyme source derived from a microorganism belonging to the genus Bacillus and capable of converting Compound (I-a) or Compound (I-b) into Compound (II-a) or Compound (II-b) in a reaction mixture to form Compound (II-a) or Compound (II-b) in the reaction mixture, and recovering Compound (II-a) or Compound (II-b) from the reaction mixture.
摘要翻译：公开了一种制备由通式（II-a）表示的化合物的方法：其中R 1表示氢原子，取代或未取代的烷基或碱金属; R 2表示取代或未取代的烷基或芳基） [化学式（II-a）]或化合物（II-a）的内酯形式[以下称为化合物（II-b）]] [其中包括使通式（Ia）表示的化合物 R1表示氢原子，取代或未取代的烷基或碱金属; R2表示取代或未取代的烷基或芳基）[以下称为化合物（Ia）]或化合物（Ia）的内酯形式[ （Ia）或化合物（Ib）转化为化合物（II-a）或化合物（II-a）的酶源的作用，以下称为化合物（Ib） b）在反应混合物中形成化合物（II -a）或化合物（II-b），并从反应混合物中回收化合物（II-a）或化合物（II-b）。

6. 发明授权

US06413748B1 Process for producing CIS-3-hydroxy-L-proline 有权
标题翻译：制备CIS-3-羟基-L-脯氨酸的方法
公开(公告)号：US06413748B1
公开(公告)日：2002-07-02
申请号：US09455406
申请日：1999-12-06
申请人： Akio Ozaki , Hideo Mori , Takeshi Shibasaki , Katsuhiko Ando , Keiko Ochiai , Shigeru Chiba , Yoichi Uosaki
发明人： Akio Ozaki , Hideo Mori , Takeshi Shibasaki , Katsuhiko Ando , Keiko Ochiai , Shigeru Chiba , Yoichi Uosaki
IPC分类号： C12P1324
CPC分类号： C12N9/0071 , C12P13/24 , C12Y114/11002 , Y10S435/814
摘要： An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.
摘要翻译：用于生产顺式-3-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品添加剂。在此过程中，L-脯氨酸在衍生自属于链霉菌属或芽孢杆菌属的微生物的酶源存在下转化为顺式-3-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化为顺式 - 3-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，在水性介质中收集生成的顺式-3-羟基-L-脯氨酸。一种新的L-脯氨酸-3-羟化酶，可用于该方法的L-脯氨酸-3-羟化酶的基因，含该基因的转化体，以及使用转化体产生L-脯氨酸-3-羟化酶的方法。

7. 发明授权

US5843955A Compound LK6-A 失效
标题翻译：复方LK6-A
公开(公告)号：US5843955A
公开(公告)日：1998-12-01
申请号：US723149
申请日：1996-09-30
申请人： Tatsuya Tamaoki , Hiroyuki Nagata , Isami Takahashi , Mayumi Yoshida , Yumiko Aotani , Katsuhiko Ando , Keiko Ochiai
发明人： Tatsuya Tamaoki , Hiroyuki Nagata , Isami Takahashi , Mayumi Yoshida , Yumiko Aotani , Katsuhiko Ando , Keiko Ochiai
IPC分类号： C07D471/06 , A61K31/475 , C07D471/16
CPC分类号： C07D471/06
摘要： The present invention relates to compound LK6-A represented by the formula: ##STR1## which has immunosuppressive activity, and pharmaceutically acceptable salts thereof.
摘要翻译：本发明涉及由下式表示的化合物LK6-A：具有免疫抑制活性的及其药学上可接受的盐。

8. 发明授权

US07112432B2 Process for producing cis-3-hydroxy-L-proline 有权
公开(公告)号：US07112432B2
公开(公告)日：2006-09-26
申请号：US10864423
申请日：2004-06-10
申请人： Akio Ozaki , Hideo Mori , Takeshi Shibasaki , Katsuhiko Ando , Keiko Ochiai , Shigeru Chiba , Yoichi Uosaki
发明人： Akio Ozaki , Hideo Mori , Takeshi Shibasaki , Katsuhiko Ando , Keiko Ochiai , Shigeru Chiba , Yoichi Uosaki
IPC分类号： C12N1/20 , C12N15/75 , C12N15/76 , C07H21/04
CPC分类号： C12N9/0071 , C12P13/24 , C12Y114/11002 , Y10S435/832 , Y10S435/886
摘要： An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.

9. 发明授权

US06767726B2 Process for producing cis-3-hydroxy-L-proline 失效
标题翻译：顺式-3-羟基-L-脯氨酸的制备方法
公开(公告)号：US06767726B2
公开(公告)日：2004-07-27
申请号：US10138632
申请日：2002-05-06
申请人： Akio Ozaki , Hideo Mori , Takeshi Shibasaki , Katsuhiko Ando , Keiko Ochiai , Shigeru Chiba , Yoichi Uosaki
发明人： Akio Ozaki , Hideo Mori , Takeshi Shibasaki , Katsuhiko Ando , Keiko Ochiai , Shigeru Chiba , Yoichi Uosaki
IPC分类号： C12N902
CPC分类号： C12N9/0071 , C12P13/24 , C12Y114/11002 , Y10S435/814
摘要： An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.
摘要翻译：分离的基因编码具有SEQ ID NO：1,2,15,16和17的氨基酸序列的L-脯氨酸-3-羟化酶，或编码具有酶活性的蛋白质，以使L-脯氨酸的3-位羟基化并在2-酮戊二酸和二价铁离子存在下作用于游离的L-脯氨酸以产生顺式-3-羟基-L-脯氨酸。将该基因插入载体中，并将所得的重组DNA用于产生转化体。通过在培养基中培养转化体产生L-脯氨酸-3-羟化酶。

10. 发明授权

US06589774B2 Process for producing a bran pickless flavoring solution 失效
公开(公告)号：US06589774B2
公开(公告)日：2003-07-08
申请号：US10062777
申请日：2002-02-05
申请人： Chiaki Saitoh , Atsushi Yashiro , Chikara Tokunaga , Kyoko Ozawa , Atsuko Yokoi , Nobuo Ogata , Hiroshi Katahira , Keiko Ochiai , Katsuhiko Ando
发明人： Chiaki Saitoh , Atsushi Yashiro , Chikara Tokunaga , Kyoko Ozawa , Atsuko Yokoi , Nobuo Ogata , Hiroshi Katahira , Keiko Ochiai , Katsuhiko Ando
IPC分类号： C12N120
CPC分类号： C12R1/15 , A23B7/10 , A23L27/24 , C12P17/04 , Y10S435/843
摘要： The present invention relates to a novel flavoring solution for making pickles having a flavor of foods pickled in rice bran paste (hereinafter referred to as a bran pickles flavoring solution), a clear bran pickles flavoring solution, processes for producing said solutions, a novel microorganism belonging to the genus Corynebacterium used for the production of said flavoring solutions, and a process for producing a solution containing a lactone wherein the lactone is selectively extracted from a culture of a lactone-producing microorganism. The present invention also relates to a novel bacterium of the genus Corynebacterium which has the ability to produce &ggr;-dodecalactone and/or &ggr;-dodecelactone and is useful for the production of said bran pickles flavoring solutions.

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