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1. 发明授权

US06797499B2 Isolated human dehydrogenases, nucleic acid molecules encoding these human dehydrogenases, and uses thereof 失效
标题翻译：分离的人脱氢酶，编码这些人脱氢酶的核酸分子及其用途
公开(公告)号：US06797499B2
公开(公告)日：2004-09-28
申请号：US10118037
申请日：2002-04-09
申请人： Fangcheng Gong , Chunhua Yan , Valentina Di Francesco , Ellen M. Beasley
发明人： Fangcheng Gong , Chunhua Yan , Valentina Di Francesco , Ellen M. Beasley
IPC分类号： C12N902
CPC分类号： C12N9/0006
摘要： The present invention provides amino acid sequences of polypeptides that are encoded by genes within the human genome, the dehydrogenase polypeptides of the present invention. The present invention specifically provides isolated polypeptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the dehydrogenase polypeptides, and methods of identifying modulators of the dehydrogenase polypeptides.
摘要翻译：本发明提供由人基因组内的基因，本发明的脱氢酶多肽编码的多肽的氨基酸序列。本发明具体提供分离的多肽和核酸分子，鉴定脱氢酶多肽的直向同源物和旁系同源物的方法，以及鉴定脱氢酶多肽的调节剂的方法。

2. 发明授权

US06790645B2 Oxidation of a cyclohexanone derivative using a brevibacterium cyclohexanone monooxygenase 失效
标题翻译：使用短纤维环己酮单加氧酶氧化环己酮衍生物
公开(公告)号：US06790645B2
公开(公告)日：2004-09-14
申请号：US10230562
申请日：2002-08-29
申请人： Patricia C. Brzostowicz , Pierre E. Rouviere
发明人： Patricia C. Brzostowicz , Pierre E. Rouviere
IPC分类号： C12N902
CPC分类号： C12Q1/689 , C12Q1/6809 , C12Q2600/158
摘要： Two gene clusters have been isolated from an Brevibacterium sp HCU that encode the enzymes expected to convert cyclohexanol to adipic acid. Individual open reading frames (ORF's) on each gene cluster are useful for the production of intermediates in the adipic acid biosynthetic pathway or of related molecules. All the ORF's have been sequenced. Identification of gene function has been made on the basis of sequence comparison and biochemical analysis.
摘要翻译：已经从Brevibacterium sp HCU中分离出两个基因簇，其编码预期将环己醇转化为己二酸的酶。每个基因簇上的单个开放阅读框（ORF）可用于生产己二酸生物合成途径或相关分子中的中间体。所有的ORF都被排序了。基于序列比较和生物化学分析的基因功能鉴定。

3. 发明授权

US06790644B2 1,3-dicarbonyl-dioxygenase—a new c-c-bond cleaving enzyme 失效
标题翻译： 1,3-二羰基双加氧酶 - 一种新的c-c键切割酶
公开(公告)号：US06790644B2
公开(公告)日：2004-09-14
申请号：US10141225
申请日：2002-05-07
申请人： Grit Straganz , Anton Glieder , Lothar Brecker
发明人： Grit Straganz , Anton Glieder , Lothar Brecker
IPC分类号： C12N902
CPC分类号： C12N9/0069
摘要： An enzyme that cleaves 1,3-dicarbonyl compounds by incorporating molecular oxygen into the substrate in amounts equimolar to the amount of substrate cleaved. The enzyme from Acinetobacter johnsonii is a multimer of about 67 kilodaltons made up of subunits of about 16.6 kilodaltons. Nucleotide and amino acid sequence analysis of a cloned A. johnsonii subunit indicates little homology with other proteins. Native or recombinant enzyme can be used to decontaminate or detoxify acetylacetone or related diketones.
摘要翻译：通过将分子氧引入底物中的量与切割的底物的量相等的量来切割1,3-二羰基化合物的酶。来自不动杆菌属的不动杆菌属的酶是由约16.6千道尔顿的亚基组成的约67千道尔顿的多聚体。克隆的约翰逊亚单位的核苷酸和氨基酸序列分析表明与其他蛋白质几乎没有同源性。天然或重组酶可用于消除乙酰丙酮或相关二酮的去污或解毒。

4. 发明授权

US06689596B2 High level expression of human cyclooxygenase-2 失效
标题翻译：人类环加氧酶-2的高水平表达
公开(公告)号：US06689596B2
公开(公告)日：2004-02-10
申请号：US10027961
申请日：2001-10-25
申请人： Gary P. O'Neill , Joseph A. Mancini
发明人： Gary P. O'Neill , Joseph A. Mancini
IPC分类号： C12N902
CPC分类号： C12N9/0083 , C07K2319/00 , C12Q1/26 , C12Y114/99001 , G01N33/5005
摘要： The invention discloses a cDNA consisting of human cyclooxygenase-2 cDNA attached to 3′ flanking sequence of human cyclooxygenase-1 methods for increasing the expression of human cyclooxygenase-2 in transformed cells and assays for preferentially and independently measuring cyclogenase-2 in samples.
摘要翻译：本发明公开了一种由人环加氧酶-2 cDNA连接的cDNA，该环加氧酶-2连接于人环氧合酶-1的3'侧翼序列，用于增加转化细胞中人环氧合酶-2的表达，并且用于优先和独立地测量样品中的环化酶-2的测定。

5. 发明授权

US06645746B1 Carbonyl reductase, gene thereof and method of using the same 有权
标题翻译：羰基还原酶，其基因及其使用方法
公开(公告)号：US06645746B1
公开(公告)日：2003-11-11
申请号：US09890821
申请日：2001-11-19
申请人： Noriyuki Kizaki , Yukio Yamada , Yoshihiko Yasohara , Junzo Hasegawa
发明人： Noriyuki Kizaki , Yukio Yamada , Yoshihiko Yasohara , Junzo Hasegawa
IPC分类号： C12N902
CPC分类号： C12N9/0006 , C12P7/62 , C12P41/00 , C12P41/002
摘要： A novel polypeptide for producing tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate, a gene encoding the polypeptide, and a method of using the polypeptide, are provided. The polypeptide has an enzyme activity to asymmetrically reduce tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. The polypeptide comprises the amino acid sequence represented by SEQ ID NO. 1 in the Sequence Listing, or the amino acid sequence having at least one amino acid substitution, insertion, deletion, or addition thereof.
摘要翻译：提供了一种用于生产（3R，5S）-6-氯-3,5-二羟基己酸叔丁酯，编码该多肽的基因以及使用该多肽的方法的新型多肽。该多肽具有不对称地将（S）-6-氯-5-羟基-3-氧代己酸叔丁酯转化为（3R，5S）-6-氯-3,5-二羟基己酸叔丁酯的酶活性。多肽包含SEQ ID NO：1所示的氨基酸序列。或序列表中具有至少一个氨基酸取代，插入，缺失或添加的氨基酸序列。

6. 发明授权

US06620603B1 Human mitogenic oxidase 失效
标题翻译：人类有丝分裂氧化酶
公开(公告)号：US06620603B1
公开(公告)日：2003-09-16
申请号：US09437568
申请日：1999-11-10
申请人： J. David Lambeth , Kathy K. Griendling , Bernard P. Lassegue , Rebecca S. Arnold , Guangjie Cheng
发明人： J. David Lambeth , Kathy K. Griendling , Bernard P. Lassegue , Rebecca S. Arnold , Guangjie Cheng
IPC分类号： C12N902
CPC分类号： C12N9/0038 , A01K2217/05 , A61K38/00 , A61K48/00 , C07K16/40 , C12N9/0004 , C12Q1/26 , C12Q1/32
摘要： The present invention relates to new genes encoding for the production of novel proteins involved in generation of reactive oxygen intermediates that affect cell division. The present invention also provides vectors containing these genes, cells transfected with these vectors, antibodies raised against these novel proteins, kits for detection, localization and measurement of these genes and proteins, and methods to determine the activity of drugs to affect the activity of the proteins of the present invention.
摘要翻译：本发明涉及编码参与影响细胞分裂的活性氧中间体生成的新蛋白质的新基因。本发明还提供含有这些基因的载体，用这些载体转染的细胞，针对这些新蛋白产生的抗体，用于检测，定位和测量这些基因和蛋白质的试剂盒，以及确定药物活性以影响其活性的方法本发明的蛋白质。

7. 发明授权

US06562609B1 Cholesterol 25-hydroxylase 有权
标题翻译：胆固醇25-羟化酶
公开(公告)号：US06562609B1
公开(公告)日：2003-05-13
申请号：US09177419
申请日：1998-10-22
申请人： David W. Russell , Erik G. Lund
发明人： David W. Russell , Erik G. Lund
IPC分类号： C12N902
CPC分类号： C12N9/0083
摘要： The invention provides methods and compositions relating to cholesterol 25-hydroxylase polypeptides having cholesterol 25-hydroxylase-specific structure and activity, related polynucleotides and modulators of cholesterol 25-hydroxylase function and serum cholesterol. The invention provides isolated cholesterol 25-hydroxylase hybridization probes and primers capable of specifically hybridizing with natural cholesterol 25-hydroxylase genes, cholesterol 25-hydroxylase-specific binding agents such as specific antibodies, agonists and antagonists, and methods of making and using the subject compositions in diagnosis (e.g. genetic hybridization screens for cholesterol 25-hydroxylase transcripts), therapy (e.g. cholesterol 25-hydroxylase inhibitors to modulate serum cholesterol) and in the biopharmaceutical industry (e.g. as immunogens, reagents for isolating natural 25-hydroxylase genes and transcripts, reagents for screening chemical libraries for lead pharmacological agents, etc.).
摘要翻译：本发明提供了涉及具有胆固醇25-羟化酶特异性结构和活性的胆固醇25-羟化酶多肽，相关多核苷酸和胆固醇25-羟化酶功能和血清胆固醇调节剂的方法和组合物。本发明提供了能够与天然胆固醇25-羟化酶基因，胆固醇25-羟化酶特异性结合剂如特异性抗体，激动剂和拮抗剂特异性杂交的分离的胆固醇25-羟化酶杂交探针和引物，以及制备和使用本发明组合物的方法在诊断中（例如胆固醇25-羟化酶转录物的遗传杂交筛选），治疗（例如调节血清胆固醇的胆固醇25-羟化酶抑制剂）和生物制药工业（例如免疫原，用于分离天然的25-羟化酶基因和转录物的试剂，试剂用于筛选铅药理学试剂的化学文库等）。

8. 发明授权

US06482621B1 Compositions and methods for fumonisin detoxification 有权
公开(公告)号：US06482621B1
公开(公告)日：2002-11-19
申请号：US09677488
申请日：2000-10-02
申请人： Jon Duvick , Joyce Maddox , Jacob Gilliam , Otto Folkerts , Oswald R. Crasta
发明人： Jon Duvick , Joyce Maddox , Jacob Gilliam , Otto Folkerts , Oswald R. Crasta
IPC分类号： C12N902
CPC分类号： C12N9/0071 , C07K14/37 , C12N9/0004 , C12N9/0012 , C12N9/0083 , C12N9/16 , C12N9/18 , C12N15/8242 , C12N15/8274
摘要： Compositions and methods for the complete detoxification of fumonisin and fumonisin degradation products are provided. Particularly, nucleotide sequences corresponding to the detoxification enzymes are provided. The sequences find use in preparing expression cassettes for the transformation of a broad variety of host cells and organisms.

9. 发明授权

US06355462B1 Disease resistance factor 有权
标题翻译：抗病因子
公开(公告)号：US06355462B1
公开(公告)日：2002-03-12
申请号：US09433248
申请日：1999-11-04
申请人： Saverio Carl Falco , Omolayo O. Famodu , Feng Han , J. Antoni Rafalski
发明人： Saverio Carl Falco , Omolayo O. Famodu , Feng Han , J. Antoni Rafalski
IPC分类号： C12N902
CPC分类号： C12N9/0004 , C07K14/415 , C12N15/8209 , C12N15/8279
摘要： This invention relates to an isolated nucleic acid fragment encoding a berberine-bridge-forming enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the berberine-bridge-forming enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the berberine-bridge-forming enzyme in a transformed host cell.
摘要翻译：本发明涉及编码小檗碱桥形成酶的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码小檗碱桥形成酶的全部或部分的嵌合基因，其中嵌合基因的表达导致产生改变的小檗碱桥形成水平酶在转化的宿主细胞中。

10. 发明授权

US06355461B2 Non-aqueous, liquid, enzyme-containing compositions 失效
标题翻译：非水，液体，含酶组合物
公开(公告)号：US06355461B2
公开(公告)日：2002-03-12
申请号：US09174202
申请日：1998-10-16
申请人： Lotte Rugholm Henriksen , Mads Lykke
发明人： Lotte Rugholm Henriksen , Mads Lykke
IPC分类号： C12N902
CPC分类号： C11D17/0004 , C11D3/38618 , C12N9/96
摘要： A substantially water-free, liquid, enzyme-containing composition comprises: (A) an enzyme; (B) a substance selected from (i) substances which in aqueous medium are substrates for said enzyme, (ii) substances which in aqueous medium are precursors for substrates for said enzyme, and (iii) substances which are cofactors for said enzyme; and (C) a non-aqueous liquid phase.
摘要翻译：基本上无水，液体的含酶组合物包含：（A）酶; （B）选自（i）在水性介质中的物质是所述酶的底物的物质，（ii）在水性介质中的物质是所述酶的底物的前体，和（iii）作为所述酶的辅因子的物质; 和（C）非水性液相。

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