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1. 发明申请

US20100159451A1 Method of detecting interaction between nucleic acid and protein, and apparatus for the same 有权
标题翻译：检测核酸与蛋白质相互作用的方法及其设备
公开(公告)号：US20100159451A1
公开(公告)日：2010-06-24
申请号：US12308370
申请日：2007-06-14
申请人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi , Hiroyuki Takamatsu
发明人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi , Hiroyuki Takamatsu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , G01N21/171 , C12Q2537/101 , C12Q2541/101
摘要： The invention is to easily detect an interaction between nucleic acid and protein with high sensitivity without the need of sample labeling with a fluorescent molecule or sample anchorage onto a metal thin-film. As means for it, the presence or absence of occurrence of the interaction between nucleic acid and protein in a sample (S) is detected in an optical manner. Specifically, the sample (S) is irradiated with excitation rays (Le) and with measuring rays (L2) for measuring a photothermal effect produced in the sample (S) through the irradiation with the excitation rays (Le). A measurement signal for the photothermal effect in the sample (S) by the excitation rays (Le) is produced on the basis of any phase change of the measuring rays (L2). A temporal variation in the measurement signal is used for making a judgment on the presence or absence of any occurrence of interaction between nucleic acid and protein.
摘要翻译：本发明可以容易地以高灵敏度检测核酸和蛋白质之间的相互作用，而不需要用荧光分子或样品锚定物将样品标记在金属薄膜上。作为其手段，以光学方式检测样品（S）中核酸和蛋白质之间的相互作用的存在或不存在。具体地，用激发光线（Le）和用于通过激发光线（Le）的照射测量样品（S）中产生的光热效应的测量光线（L2）来照射样品（S）。基于测量光线（L2）的任何相变，产生通过激发光线（Le）在样品（S）中的光热效应的测量信号。使用测量信号的时间变化来判断核酸和蛋白质之间是否存在任何相互作用的发生。

2. 发明授权

US08367329B2 Method of detecting interaction between nucleic acid and protein, and apparatus for the same 有权
标题翻译：检测核酸与蛋白质相互作用的方法及其设备
公开(公告)号：US08367329B2
公开(公告)日：2013-02-05
申请号：US12308370
申请日：2007-06-14
申请人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi , Hiroyuki Takamatsu
发明人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi , Hiroyuki Takamatsu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , G01N21/171 , C12Q2537/101 , C12Q2541/101
摘要： The invention is to easily detect an interaction between nucleic acid and protein with high sensitivity without the need of sample labeling with a fluorescent molecule or sample anchorage onto a metal thin-film. As means for it, the presence or absence of occurrence of the interaction between nucleic acid and protein in a sample (S) is detected in an optical manner. Specifically, the sample (S) is irradiated with excitation rays (Le) and with measuring rays (L2) for measuring a photothermal effect produced in the sample (S) through the irradiation with the excitation rays (Le). A measurement signal for the photothermal effect in the sample (S) by the excitation rays (Le) is produced on the basis of any phase change of the measuring rays (L2). A temporal variation in the measurement signal is used for making a judgment on the presence or absence of any occurrence of interaction between nucleic acid and protein.
摘要翻译：本发明可以容易地以高灵敏度检测核酸和蛋白质之间的相互作用，而不需要用荧光分子或样品锚定物将样品标记在金属薄膜上。作为其手段，以光学方式检测样品（S）中核酸和蛋白质之间的相互作用的存在或不存在。具体地，用激发光线（Le）和用于通过激发光线（Le）的照射测量样品（S）中产生的光热效应的测量光线（L2）来照射样品（S）。基于测量光线（L2）的任何相变，产生通过激发光线（Le）在样品（S）中的光热效应的测量信号。使用测量信号的时间变化来判断核酸和蛋白质之间是否存在任何相互作用的发生。

3. 发明授权

US08310676B2 Method and apparatus for detecting small biomolecules 有权
标题翻译：检测小生物分子的方法和装置
公开(公告)号：US08310676B2
公开(公告)日：2012-11-13
申请号：US12667585
申请日：2008-07-03
申请人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi
发明人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi
IPC分类号： G01N21/00
CPC分类号： G01N33/54373 , B01L3/5027 , B01L7/00 , B01L2300/0654 , B01L2300/0861 , B01L2300/0877 , B01L2300/0883 , B01L2300/1827 , G01N21/171 , G01N35/1095 , G01N2035/1034
摘要： An object of the present invention is to detect a small biomolecule in a sample using simple and inexpensive equipment. To achieve this, the small biomolecule in a sample S is detected optically. Specifically, the sample S containing an aptamer capable of interacting with the small biomolecule is irradiated with an excitation light Le and irradiated with a measurement light L2 for measuring the photothermal effect produced in the sample S by the irradiation with the excitation light Le. The photothermal effect induced in the sample S by the excitation light Le is measured from the phase change in the measurement light L2, and the presence or absence of the interaction between the biomolecule and the aptamer is assessed based on the temporal variation in the measurement signal.
摘要翻译：本发明的目的是使用简单且便宜的设备来检测样品中的小生物分子。为了实现这一点，光学检测样品S中的小生物分子。具体地说，将含有能够与小生物分子相互作用的适体的样品S用激发光Le1照射，并用用于通过照射激发光Le测量在样品S中产生的光热效应的测量光L2照射。根据测量光L2的相变测量由激发光Le引起的样品S中的光热效应，并且基于测量信号的时间变化来评估生物分子和适体之间的相互作用的存在或不存在。

4. 发明申请

US20110007317A1 METHOD AND APPARATUS FOR DETECTING SMALL BIOMOLECULES 有权
标题翻译：用于检测小生物分子的方法和装置
公开(公告)号：US20110007317A1
公开(公告)日：2011-01-13
申请号：US12667585
申请日：2008-07-03
申请人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi
发明人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi
IPC分类号： G01N21/00
CPC分类号： G01N33/54373 , B01L3/5027 , B01L7/00 , B01L2300/0654 , B01L2300/0861 , B01L2300/0877 , B01L2300/0883 , B01L2300/1827 , G01N21/171 , G01N35/1095 , G01N2035/1034
摘要： An object of the present invention is to detect a small biomolecule in a sample using simple and inexpensive equipment. To achieve this, the small biomolecule in a sample S is detected optically. Specifically, the sample S containing an aptamer capable of interacting with the small biomolecule is irradiated with an excitation light Le and irradiated with a measurement light L2 for measuring the photothermal effect produced in the sample S by the irradiation with the excitation light Le. The photothermal effect induced in the sample S by the excitation light Le is measured from the phase change in the measurement light L2, and the presence or absence of the interaction between the biomolecule and the aptamer is assessed based on the temporal variation in the measurement signal.
摘要翻译：本发明的目的是使用简单且便宜的设备来检测样品中的小生物分子。为了实现这一点，光学检测样品S中的小生物分子。具体地说，将含有能够与小生物分子相互作用的适体的样品S用激发光Le1照射，并用用于通过照射激发光Le测量在样品S中产生的光热效应的测量光L2照射。根据测量光L2的相变测量由激发光Le引起的样品S中的光热效应，并且基于测量信号的时间变化来评估生物分子和适体之间的相互作用的存在或不存在。

5. 发明授权

US08143004B2 Method of assaying target substance in sample, aptamer molecule method of constructing the same 失效
标题翻译：测定样品中目标物质的方法，适体分子构建方法
公开(公告)号：US08143004B2
公开(公告)日：2012-03-27
申请号：US12443053
申请日：2007-09-27
申请人： Kazunori Ikebukuro , Koji Sode
发明人： Kazunori Ikebukuro , Koji Sode
IPC分类号： C12Q1/68
CPC分类号： G01N33/5308
摘要： A method for measuring a test substance whereby the test substance in a sample can be specifically measured without using an antibody against the test substance; an aptamer molecule used therefor; and a method for creating the aptamer are disclosed. An aptamer capable of hybridizing with an oligonucleotide when it is bound to a test substance, but is incapable of hybridizing with the oligonucleotide when it is not bound to the test substance, is utilized. The aptamer is brought into contact with a sample, and the aptamer bound to the test substance is brought into contact with an immobilized oligonucleotide which hybridizes with the aptamer, to bind the aptamer to a solid phase, followed by measurement of the aptamer immobilized on the solid phase.
摘要翻译：一种测试物质的方法，其中可以特异性地测量样品中的测试物质，而不使用针对测试物质的抗体; 用于其的适体分子; 并且公开了用于产生适配体的方法。当与被检物质结合时能够与寡核苷酸杂交，但是当不与被检物质结合时，不能与寡核苷酸杂交的适体。使适体与样品接触，使与测试物质结合的适配体与与适体杂交的固定化寡核苷酸接触，将适体结合固相，随后测定固定在固相。

6. 发明授权

US09238816B2 Amyloid protein oligomer-binding aptamer 有权
标题翻译：淀粉样蛋白寡聚物结合适体
公开(公告)号：US09238816B2
公开(公告)日：2016-01-19
申请号：US14000190
申请日：2012-02-20
申请人： Kazunori Ikebukuro , Kaori Tsukakoshi , Koji Sode
发明人： Kazunori Ikebukuro , Kaori Tsukakoshi , Koji Sode
IPC分类号： C12N15/115 , G01N33/68
CPC分类号： C12N15/115 , C12N2310/16 , C12N2310/50 , G01N33/6896
摘要： An aptamer having a G-quartet structure and being at least one selected from the group consisting of the following polynucleotides, the aptamer having a binding ability to an amyloid protein oligomer: (1) a polynucleotide comprising a base sequence represented by any one of SEQ ID NO:1 to SEQ ID NO:18; (2) a polynucleotide comprising a base sequence that includes at least four sets of at least two consecutive guanosine nucleotides and in which one or several bases has been deleted, substituted, or added in a base sequence represented by any one of SEQ ID NO:18; and (3) a polynucleotide that is a multimer including the polynucleotide of (1) or (2) as a structural unit.
摘要翻译：具有G-四重结构并且选自下列多核苷酸中的至少一种的适体，所述适配体具有与淀粉样蛋白寡聚物的结合能力：（1）包含由SEQ ID NO： SEQ ID NO：1至SEQ ID NO：18; （2）包含碱基序列的多核苷酸，其包含至少两组至少两个连续的鸟苷核苷酸，并且其中一个或几个碱基已被缺失，取代或加入到由SEQ ID NO： 18; 和（3）多核苷酸，其是包含作为结构单元的（1）或（2）的多核苷酸的多聚体。

7. 发明申请

US20140011207A1 PSA BINDING APTAMER AND METHOD FOR DIAGNOSIS OF PROSTATE CANCER 审中-公开
标题翻译： PSA结合APTAMER和诊断前列腺癌的方法
公开(公告)号：US20140011207A1
公开(公告)日：2014-01-09
申请号：US13806998
申请日：2010-07-02
申请人： Kazunori Ikebukuro
发明人： Kazunori Ikebukuro
IPC分类号： C12N15/115 , G01N33/574
CPC分类号： C12N15/115 , C12N2310/16 , G01N33/57434 , G01N33/57488
摘要： The present invention provides an aptamer that binds to prostate-specific antigen (PSA), the aptamer including: a preceding region having a random polynucleotide sequence consisting of from 1 to 10 nucleotides; a first region, at a 3′ end of the preceding region, consisting of nnnnCT wherein each n is independently selected from A, T, G and C; a second region consisting of nnCTTT wherein each n is independently selected from A, T, G and C, and at least one part of the second region is complementary to the first region; and a third region positioned between the first region and the second region and consisting of a random polynucleotide sequence having from 3 to 30 nucleotides.
摘要翻译：本发明提供了与前列腺特异性抗原（PSA）结合的适体，所述适体包括：具有1至10个核苷酸组成的随机多核苷酸序列的前一区域; 在前一区域的3'端的第一区域，由nnnnCT组成，其中每个n独立地选自A，T，G和C; 由nnCTTT组成的第二区域，其中每个n独立地选自A，T，G和C，并且第二区域的至少一部分与第一区域互补; 以及位于第一区域和第二区域之间的由具有3至30个核苷酸的随机多核苷酸序列组成的第三区域。

8. 发明申请

US20110045484A1 METHOD OF DETECTING TARGET MOLECULE BY USING APTAMER 有权
标题翻译：通过使用APTAMER检测目标分子的方法
公开(公告)号：US20110045484A1
公开(公告)日：2011-02-24
申请号：US12874202
申请日：2010-09-01
申请人： Koji SODE , Kazunori Ikebukuro
发明人： Koji SODE , Kazunori Ikebukuro
IPC分类号： C12Q1/68
CPC分类号： G01N33/54393 , C12Q1/6816 , G01N33/543 , C12Q2525/161 , C12Q2525/205 , C12Q2565/107
摘要： An aptamer-probe complex for detecting the presence of a target molecule is disclosed. The complex of the present invention contains an aptamer moiety which is able to bind to an indicator protein and change the properties of the indicator protein, and a probe moiety which is able to bind to a target molecule, wherein the aptamer moiety and the probe moiety are combined in such a manner that the binding mode between the aptamer moiety and the indicator protein changes when the probe moiety binds to the target molecule. A target molecule can be detected with combination of an aptamer which binds to a certain protein, and a probe which binds to the target molecule, utilizing the properties of that protein as an indicator.
摘要翻译：公开了一种用于检测靶分子存在的适体 - 探针复合物。本发明的复合物包含能够结合指示蛋白并改变指示蛋白的性质的适体部分，以及能够结合靶分子的探针部分，其中适体部分和探针部分以使得当探针部分结合到靶分子时，适体部分和指示蛋白之间的结合模式发生变化的方式组合。可以通过结合某种蛋白质的适配体的组合和结合靶分子的探针，利用该蛋白质的特性作为指标来检测靶分子。

9. 发明申请

US20100021899A1 METHOD OF ASSAYING TARGET SUBSTANCE IN SAMPLE, APTAMER MOLECULE AND METHOD OF CONSTRUCTING THE SAME 失效
标题翻译：测定样品中目标物质的方法，APTAMER分子及其构建方法
公开(公告)号：US20100021899A1
公开(公告)日：2010-01-28
申请号：US12443053
申请日：2007-09-27
申请人： Kazunori Ikebukuro , Koji Sode
发明人： Kazunori Ikebukuro , Koji Sode
IPC分类号： C12Q1/68 , C12N15/115
CPC分类号： G01N33/5308
摘要： A method for measuring a test substance whereby the test substance in a sample can be specifically measured without using an antibody against the test substance; an aptamer molecule used therefor; and a method for creating the aptamer are disclosed. An aptamer capable of hybridizing with an oligonucleotide when it is bound to a test substance, but is incapable of hybridizing with the oligonucleotide when it is not bound to the test substance, is utilized. The aptamer is brought into contact with a sample, and the aptamer bound to the test substance is brought into contact with an immobilized oligonucleotide which hybridizes with the aptamer, to bind the aptamer to a solid phase, followed by measurement of the aptamer immobilized on the solid phase.
摘要翻译：一种测试物质的方法，其中可以特异性地测量样品中的测试物质，而不使用针对测试物质的抗体; 用于其的适体分子; 并且公开了用于产生适配体的方法。当与被检物质结合时能够与寡核苷酸杂交，但是当不与被检物质结合时，不能与寡核苷酸杂交的适体。使适体与样品接触，使与测试物质结合的适配体与与适体杂交的固定化寡核苷酸接触，将适体结合固相，随后测定固定在固相。

10. 发明申请

US20140072986A1 AMYLOID PROTEIN OLIGOMER-BINDING APTAMER 有权
标题翻译： AMYLOID蛋白低聚蛋白APTAMER
公开(公告)号：US20140072986A1
公开(公告)日：2014-03-13
申请号：US14000190
申请日：2012-02-20
申请人： Kazunori Ikebukuro , Kaori Tsukakoshi , Koji Sode
发明人： Kazunori Ikebukuro , Kaori Tsukakoshi , Koji Sode
IPC分类号： C12N15/115 , G01N33/68
CPC分类号： C12N15/115 , C12N2310/16 , C12N2310/50 , G01N33/6896
摘要： An aptamer having a G-quartet structure and being at least one selected from the group consisting of the following polynucleotides, the aptamer having a binding ability to an amyloid protein oligomer: (1) a polynucleotide comprising a base sequence represented by any one of SEQ ID NO:1 to SEQ ID NO:18; (2) a polynucleotide comprising a base sequence that includes at least four sets of at least two consecutive guanosine nucleotides and in which one or several bases has been deleted, substituted, or added in a base sequence represented by any one of SEQ ID NO:18; and (3) a polynucleotide that is a multimer including the polynucleotide of (1) or (2) as a structural unit.
摘要翻译：具有G-四重结构并且选自下列多核苷酸中的至少一种的适体，所述适配体具有与淀粉样蛋白寡聚物的结合能力：（1）包含由SEQ ID NO： SEQ ID NO：1至SEQ ID NO：18; （2）包含碱基序列的多核苷酸，其包含至少两组至少两个连续的鸟苷核苷酸，并且其中一个或几个碱基已被缺失，取代或加入到由SEQ ID NO： 18; 和（3）多核苷酸，其是包含作为结构单元的（1）或（2）的多核苷酸的多聚体。

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