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1. 发明授权

US5211829A Analytical method and apparatus using capillary tube 失效
标题翻译：使用毛细管的分析方法和装置
公开(公告)号：US5211829A
公开(公告)日：1993-05-18
申请号：US664604
申请日：1991-03-04
申请人： Kazumichi Imai , Masataka Koga , Takehiko Kitamori , Tsuguo Sawada , Jiaqi Wu
发明人： Kazumichi Imai , Masataka Koga , Takehiko Kitamori , Tsuguo Sawada , Jiaqi Wu
IPC分类号： G01N25/00 , G01N21/00 , G01N27/447 , G01N29/00 , G01N30/62 , G01N30/74 , G01N30/76 , G01N35/08
CPC分类号： G01N30/76 , G01N27/44717
摘要： The present invention is applied to an analyzer according to which a sample to be analyzed is moved through a capillary tube in liquid chromatography or capillary-zone electrophoretic method. The capillary tube has a separation zone and a detection zone. The capillary tube in the detection zone has a section in which it can vibrate and both ends of this section are fixed.Analytes of the sample introduced into the analyzer are separated from each other in the separation zone. When each of the separated analytes is led to the area irradiated with beam in the detection zone, the capillary tube in the detection zone vibrates. This vibration phenomenon is brought about by tension fluctuation of the capillary tube caused by intermittent irradiation of the detection zone with an excitation beam and absorption of the excitation beam by the analyte. Concentration of analytes in the sample can be measured by detecting amplitude of the vibration.
摘要翻译：本发明应用于分析器，通过液相色谱法或毛细管区电泳方法将待分析样品移动通过毛细管。毛细管具有分离区和检测区。检测区域中的毛细管具有能够振动的部分，并且该部分的两端固定。在分离区中引入分析仪的样品的分析物彼此分离。当每个分离的分析物被引导到在检测区域中用光束照射的区域时，检测区域中的毛细管管振动。这种振动现象是由于由激发束间歇地照射检测区域而引起的毛细管的张力波动和被分析物吸收激发光束引起的。可以通过检测振动的幅度来测量样品中分析物的浓度。

2. 发明授权

US5365559A Particle counting apparatus for a total counting of particles contained in a liquid sample 失效
标题翻译：用于对包含在液体样品中的颗粒的总计数的颗粒计数装置
公开(公告)号：US5365559A
公开(公告)日：1994-11-15
申请号：US8984
申请日：1993-01-26
申请人： Yu-Ming Hsueh , Kazumichi Imai , Masataka Koga
发明人： Yu-Ming Hsueh , Kazumichi Imai , Masataka Koga
IPC分类号： G01N15/14 , G01N15/02 , G01N29/00 , G06M1/10 , G06M11/00 , G01N15/00 , G01N21/53
CPC分类号： G01N15/0205 , G06M1/101 , G06M11/00
摘要： A particle counting apparatus for counting a total particle counting value contained in a liquid sample comprising, a flow cell in which the liquid sample flows, a light source for irradiating a light beam to the sample liquid in the flow cell, a detector for detecting pulse-wise signal scattered from the particles by the irradiating of the light beam, and a computer for obtaining the total particle counting value contained in the liquid sample as a counting value measured when the top end of the liquid sample is irradiated with the light beam and the plural particles in the top end does not overlapped in the light beam, multiplied by a coefficient.
摘要翻译：一种粒子计数装置，用于计数包含在液体样品中的总颗粒计数值，该液体样品包括：液体样品流动的流动池，用于向流动池中的样品液体照射光束的光源;检测脉冲的检测器通过照射光束从颗粒散射的信号;以及计算机，用于获得液体样品中包含的总颗粒计数值作为当用光束照射液体样品的顶端时测量的计数值，以及顶端的多个颗粒在光束中不重叠，乘以系数。

3. 发明申请

US20130157264A1 NUCLEIC ACID ANALYSIS DEVICE, NUCLEIC ACID ANALYSIS APPARATUS, AND NUCLEIC ACID ANALYSIS METHOD 审中-公开
标题翻译：核酸分析装置，核酸分析装置和核酸分析方法
公开(公告)号：US20130157264A1
公开(公告)日：2013-06-20
申请号：US13818704
申请日：2011-07-19
申请人： Takayuki Obara , Kazumichi Imai , Toshiro Saito , Satoshi Takahashi
发明人： Takayuki Obara , Kazumichi Imai , Toshiro Saito , Satoshi Takahashi
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N21/648 , C12Q2563/173
摘要： In a nucleic acid analysis device which detects a fluorescent dye on a nucleic acid sample immobilized on a surface of a substrate by exciting the fluorescent dye with an evanescent wave, the detection of a fluorescence signal with a high SN ratio is realized even for a long nucleic acid sample.The nucleic acid analysis device according to the invention is a nucleic acid analysis device in which a plurality of regions for immobilizing a nucleic acid sample are provided on a surface of a support base and a single molecule of a nucleic acid sample is immobilized on at least one of the regions, and which performs sequence determination by performing an extension reaction of the immobilized nucleic acid sample, wherein the immobilization of the single molecule of the nucleic acid sample on the support base is performed at two or more points.
摘要翻译：在通过用ev逝波激发荧光染料来检测固定在基板表面上的核酸样品上的荧光染料的核酸分析装置中，即使长时间地实现高SN比的荧光信号的检测核酸样品。根据本发明的核酸分析装置是核酸分析装置，其中在支持基底的表面上提供用于固定核酸样品的多个区域，并且将至少一个核酸样品的单个分子固定在至少其中一个区域，并且通过进行固定化的核酸样品的延伸反应进行序列测定，其中将核酸样品的单分子固定在载体基底上进行两点或更多点。

4. 发明授权

US07662269B2 Capillary electrophoretic instrument and capillary array assembly 有权
标题翻译：毛细管电泳仪和毛细管阵列组件
公开(公告)号：US07662269B2
公开(公告)日：2010-02-16
申请号：US12359115
申请日：2009-01-23
申请人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
发明人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
IPC分类号： G01N27/453
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译：通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

5. 发明申请

US20070278101A1 Capillary electrophoresis apparatus 有权
标题翻译：毛细管电泳仪
公开(公告)号：US20070278101A1
公开(公告)日：2007-12-06
申请号：US11806889
申请日：2007-06-05
申请人： Takashi Gomi , Ryoji Inaba , Motohiro Yamazaki , Hidenori Namba , Jin Matsumura , Hiromi Yamashita , Seiichi Ugai , Kazumichi Imai
发明人： Takashi Gomi , Ryoji Inaba , Motohiro Yamazaki , Hidenori Namba , Jin Matsumura , Hiromi Yamashita , Seiichi Ugai , Kazumichi Imai
IPC分类号： G01N27/00
CPC分类号： G01N27/44721
摘要： The present invention provides a capillary electrophoresis apparatus in which a capillary is easily attached to and detached from a migration medium filling unit without mixing impurities into the capillary. Mixture of impurities such as dust is also prevented when a capillary negative-electrode end is brought into contact with a sample stored in a vessel. Furthermore, temperature control is efficiently performed in the capillary. In the capillary electrophoresis apparatus, the whole of capillary array can be supported by grasping a grip portion by hand. A migration medium filling mechanism includes a slide mechanism which moves a polymer block with respect to a capillary head. The capillary electrophoresis apparatus includes a vessel in which a sample and a buffer can simultaneously be stored. Temperatures of the capillary and an irradiation and detection unit are controlled by a temperature control function provided in a thermostatic device.
摘要翻译：本发明提供了一种毛细管电泳装置，其中毛细管容易地附着到迁移介质填充单元上并从迁移介质填充单元中分离而不将杂质混入毛细管中。当毛细管负极端与存储在容器中的样品接触时，也可以防止诸如灰尘等杂质的混合物。此外，在毛细管中有效地进行温度控制。在毛细管电泳装置中，通过用手抓握把手部分可以支撑整个毛细管阵列。迁移介质填充机构包括相对于毛细管头移动聚合物嵌段的滑动机构。毛细管电泳装置包括可以同时存储样品和缓冲液的容器。毛细管和照射和检测单元的温度由恒温装置中提供的温度控制功能控制。

6. 发明授权

US06191425B1 Multicolor fluorescence detection type electrophoretic analyzer 失效
标题翻译：多色荧光检测型电泳分析仪
公开(公告)号：US06191425B1
公开(公告)日：2001-02-20
申请号：US09023561
申请日：1998-02-13
申请人： Kazumichi Imai
发明人： Kazumichi Imai
IPC分类号： G01N2726
CPC分类号： G01N27/44721
摘要： A plurality of samples are separated into sample components by means of electrophoresis in a plurality of capillaries. Light is radiated onto the separated sample components. Fluorescence emitted from the sample components is measured by a detector unit. In the detector unit, the fluorescence from the sample components is collected by a lens, filtered by a filter set to select light of predetermined wavelengths, split into four bundle of rays by right-angle prisms, and focussed on four linear array sensors so as to be detected.
摘要翻译：多个样品通过多个毛细管中的电泳分离成样品成分。光照射到分离的样品组分上。通过检测器单元测量从样品组分发射的荧光。在检测器单元中，来自样品组分的荧光由透镜收集，由滤光片组滤波，以选择预定波长的光，通过直角棱镜分成四束光束，并聚焦在四个线性阵列传感器上，以便被检测。

7. 发明授权

US09207235B2 Nucleic acid analyzer, reaction device for nucleic acid analysis and substrate of reaction device for nucleic acid analysis 有权
标题翻译：核酸分析仪，核酸分析反应装置和核酸分析反应装置底物
公开(公告)号：US09207235B2
公开(公告)日：2015-12-08
申请号：US13575564
申请日：2010-12-01
申请人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
发明人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
IPC分类号： B01L3/00 , G01N33/543 , G01N21/64 , G01N33/53 , G01N33/58 , G01N35/00
CPC分类号： G01N33/54313 , B01L3/502715 , B01L2200/0668 , B01L2300/0609 , B01L2300/0877 , B01L2300/0883 , G01N21/6452 , G01N21/648 , G01N33/5308 , G01N33/54373 , G01N33/582 , G01N35/00 , G01N2035/00564
摘要： Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).
摘要翻译：提供了用于核酸分析的反应装置，其中携带已被固定在其上的被检测核酸的微粒根据二维传感器的像素尺寸以格子形式排列在基板上。通过在基板（101）上设置有通道形成反应室的核酸分析用反应装置，检测固定在基板（101）上的微粒（103）上的核酸。携带已被固定在其上的被检测核酸的微粒（103）通过在基板（101）上排列的微结构（102）布置。

8. 发明申请

US20130309675A1 Microparticle having single-molecule nucleic acid probe, method for producing same, method for analyzing nucleic acid using same, device for nucleic acid analysis, and apparatus for analyzing nucleic acid 有权
标题翻译：具有单分子核酸探针的微粒，其制备方法，使用其的核酸分析方法，核酸分析装置和核酸分析装置
公开(公告)号：US20130309675A1
公开(公告)日：2013-11-21
申请号：US13981983
申请日：2012-01-26
申请人： Toshiro Saito , Kazumichi Imai , Takayuki Obara , Eri Tarasawa
发明人： Toshiro Saito , Kazumichi Imai , Takayuki Obara , Eri Tarasawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6834 , C12Q1/6876 , C12Q2525/131 , C12Q2525/197 , C12Q2535/122 , C12Q2537/125 , C12Q2537/159 , C12Q2563/155
摘要： A microparticle having a probe molecule able to capture a specific nucleic acid group to be analyzed is used to extract only the specific nucleic acid group to be analyzed from a nucleic acid sample and the microparticle is thereafter directly immobilized on a smooth plate, whereby a device for nucleic acid analysis is rapidly prepared. Immobilizing a single capture probe molecule onto an individual microparticle in advance and forming, at regular positions on the smooth substrate, an adhesion pad on which a functional group that binds to the microparticle has been introduced makes it possible to readily and rapidly prepare the device for nucleic analysis, where the nucleic acid sample to be analyzed is arranged molecule by molecule in a lattice shape on the smooth substrate.
摘要翻译：使用具有能够捕获要分析的特定核酸组的探针分子的微粒从核酸样品中仅提取待分析的特异性核酸组，然后将微粒直接固定在光滑平板上，用于核酸分析快速准备。将单个捕获探针分子预先固定在单个微粒上，并且在光滑基底上的规则位置处形成粘附有与微粒结合的官能团的粘合垫，使得可以容易且快速地制备用于核酸分析，其中要分析的核酸样品以平滑基底上的格子状分子排列。

9. 发明授权

US08545686B2 Electrophoretic apparatus and electrophoretic method 有权
标题翻译：电泳仪和电泳法
公开(公告)号：US08545686B2
公开(公告)日：2013-10-01
申请号：US11397890
申请日：2006-04-05
申请人： Ryoji Inaba , Tomoyuki Sakai , Motohiro Yamazaki , Takashi Gomi , Kazumichi Imai
发明人： Ryoji Inaba , Tomoyuki Sakai , Motohiro Yamazaki , Takashi Gomi , Kazumichi Imai
IPC分类号： G01N27/453 , G01N27/447
CPC分类号： G01N27/44721 , G01N27/44782
摘要： The present invention relates to detection of an emission spectrum by irradiating excitation light onto a plurality of electrophoretic paths and dispersing fluorescent light output from the electrophoretic paths in a direction approximately vertical to an electrophoretic direction. According to the invention, since an emission spectrum to be detected does not substantially change over time, it becomes possible to make observed emission spectrums completely correspond to various fluorescent dyes or various bases.
摘要翻译：本发明涉及通过将激发光照射到多个电泳路径上并且将电泳路径输出的荧光以大致垂直于电泳方向的方向分散的方式来检测发射光谱。根据本发明，由于要检测的发射光谱随时间基本上不变化，因此可以使观察到的发射光谱完全对应于各种荧光染料或各种基底。

10. 发明申请

US20090134030A1 Capillary Electrophoretic Instrument and Capillary Array Assembly 有权
标题翻译：毛细管电泳仪器和毛细管阵列组件
公开(公告)号：US20090134030A1
公开(公告)日：2009-05-28
申请号：US12359115
申请日：2009-01-23
申请人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
发明人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
IPC分类号： G01N27/447
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译：通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

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