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1. 发明申请

US20070141028A1 Cancer gene therapeutic drug 审中-公开
标题翻译：癌症基因治疗药
公开(公告)号：US20070141028A1
公开(公告)日：2007-06-21
申请号：US10575894
申请日：2004-10-15
申请人： Katsuyuki Hamada , Akinobu Goto , Toshiro Shirakawa
发明人： Katsuyuki Hamada , Akinobu Goto , Toshiro Shirakawa
IPC分类号： A61K48/00 , C12N15/86 , A61K31/409 , A61K31/555
CPC分类号： C12N7/00 , A61K31/409 , A61K35/761 , A61K38/193 , A61K48/0058 , A61K2039/5256 , A61K2039/57 , C07K14/475 , C12N15/85 , C12N15/86 , C12N2710/10332 , C12N2710/10343 , C12N2830/008 , C12N2830/85 , A61K2300/00
摘要： A cancer gene therapeutic drug of the present invention includes a carrier cell to be infected with an oncolytic virus, so as to make the oncolytic virus act on a tumor cell within a living body, wherein the carrier cell is selected from A549 cell, 293 cell, SW626 cell and HT-3 cell, showing a potent antitumor effect. Because of use of such cell line having high antitumor effect both in vitro and in vivo as the carrier cell, the cancer gene therapeutic drug provides more potent antitumor effect in comparison to those of conventional carrier cells.
摘要翻译：本发明的癌症基因治疗药物包括用溶瘤病毒感染的载体细胞，使得溶瘤病毒作用于活体内的肿瘤细胞，其中载体细胞选自A549细胞，293细胞，SW626细胞和HT-3细胞，显示出有效的抗肿瘤作用。由于在体外和体内作为载体细胞使用具有高抗肿瘤作用的细胞系，与常规载体细胞相比，癌症基因治疗药物提供更有效的抗肿瘤效果。

2. 发明申请

US20070134202A1 Cancer gene therapeutic drug 审中-公开
标题翻译：癌症基因治疗药
公开(公告)号：US20070134202A1
公开(公告)日：2007-06-14
申请号：US10576047
申请日：2003-10-15
申请人： Katsuyuki Hamada , Akinobu Goto , Toshiro Shirakawa
发明人： Katsuyuki Hamada , Akinobu Goto , Toshiro Shirakawa
IPC分类号： A61K48/00 , C12N15/86
CPC分类号： C12N7/00 , A61K31/409 , A61K35/761 , A61K38/193 , A61K48/0058 , A61K2039/5256 , A61K2039/57 , C07K14/475 , C12N15/85 , C12N15/86 , C12N2710/10332 , C12N2710/10343 , C12N2830/008 , C12N2830/85 , A61K2300/00
摘要： A cancer gene therapeutic drug of the present invention comprises a combination of: a virus for immunological treatment to be administered for inducing a CTL reaction within a living body to administration of a carrier cell; and a carrier cell to be infected with an oncolytic virus before the administration so as to make the oncolytic virus act on a tumor cell within the living body. For example, A549 cell can be used as the carrier cell. Non-proliferative adenovirus inactivated by UV irradiation can be used as the virus for immunological treatment, and a proliferative adenovirus having a tumor-specific promoter can be used as the oncolytic virus, for example.
摘要翻译：本发明的癌症基因治疗药物包含以下组合：用于在活体内诱导CTL反应而施用的用于免疫治疗的病毒，用于施用载体细胞; 以及在施用前被感染有溶瘤病毒的载体细胞，以使得溶瘤病毒作用于活体内的肿瘤细胞。例如，可以使用A549细胞作为载体细胞。通过UV辐射灭活的非增殖性腺病毒可以用作免疫治疗的病毒，例如可以使用具有肿瘤特异性启动子的增殖性腺病毒作为溶瘤病毒。

3. 发明申请

US20050176138A1 Apparatus for cell culture 失效
标题翻译：细胞培养装置
公开(公告)号：US20050176138A1
公开(公告)日：2005-08-11
申请号：US11048559
申请日：2005-02-01
申请人： Takahiro Nishimoto , Yoshiaki Hamada , Seiichi Sakuramoto , Keiichi Yokouchi , Akinobu Goto , Toshiro Shirakawa
发明人： Takahiro Nishimoto , Yoshiaki Hamada , Seiichi Sakuramoto , Keiichi Yokouchi , Akinobu Goto , Toshiro Shirakawa
IPC分类号： C12M1/00 , C12M1/02 , C12M3/00
CPC分类号： C12M47/02 , C12M23/50 , C12M33/06 , C12M37/00 , C12M41/48
摘要： An object of the present invention is to provide an apparatus for cell culture exhibiting favorable property to occupy little space and economical efficiency, capable of reducing the working labor, faulty manipulation and the like, and of promoting the prevention of contamination, which is suitable for small scale cell culture at an experimental level on research, in particular. The present invention is an apparatus for cell culture wherein at least a medium replacement process and a passage culture process can be executed, and characterized by including an aspirator unit for drawing a liquid, a chemical liquid supply unit for supplying a medium, a liquid for detachment, a washing liquid and the like, a pipetting unit for quantitatively aspirating and discharging the liquid, a centrifugal unit for separating cells from the cell suspension in a centrifuge tube, and a handling unit for moving a culture vessel and the centrifuge tube to each unit; and all units being constructed such that they can be stored in a clean bench. Further, according to the present invention, the apparatus may include a clean bench having a sterile operation space, and the aforementioned units may be stored within the sterile operation space in a removable manner.
摘要翻译：本发明的目的是提供一种细胞培养装置，其具有良好的性能，占用空间少，经济效益好，能够减少劳动力，操作不良等，促进防止污染，适用于特别是在实验水平的小规模细胞培养研究。本发明是一种用于细胞培养的装置，其中至少可以执行培养基更换过程和通道培养过程，其特征在于包括用于抽取液体的抽吸单元，用于供给培养基的药液供应单元，用于洗涤液等，用于定量抽吸和排出液体的移液单元，用于在离心管中从细胞悬浮液中分离细胞的离心单元和用于将培养容器和离心管移动到每个的操作单元单元; 并且所有单元被构造成使得它们可以被存储在干净的工作台中。此外，根据本发明，该装置可以包括具有无菌操作空间的清洁台，并且上述单元可以以可移除的方式存储在无菌操作空间内。

4. 发明授权

US07419819B2 Apparatus for cell culture 失效
标题翻译：细胞培养装置
公开(公告)号：US07419819B2
公开(公告)日：2008-09-02
申请号：US11048559
申请日：2005-02-01
申请人： Takahiro Nishimoto , Yoshiaki Hamada , Seiichi Sakuramoto , Keiichi Yokouchi , Akinobu Goto , Toshiro Shirakawa
发明人： Takahiro Nishimoto , Yoshiaki Hamada , Seiichi Sakuramoto , Keiichi Yokouchi , Akinobu Goto , Toshiro Shirakawa
IPC分类号： C12M1/36 , C12M1/38 , C12M3/00
CPC分类号： C12M47/02 , C12M23/50 , C12M33/06 , C12M37/00 , C12M41/48
摘要： Apparatus for cell culture occupying little space, providing economical efficiency, reducing working labor, faulty manipulation, and promoting the prevention of contamination, which is suitable for small scale cell culture . The apparatus permits the execution of at least a medium replacement process and a passage culture process, and includes an aspirator unit for drawing a liquid, a chemical liquid supply unit for supplying a medium, a liquid for detachment, a washing liquid, a pipetting unit for quantitatively aspirating and discharging the liquid, a centrifugal unit for separating cells from the cell suspension in a centrifuge tube, and a handling unit for moving a culture vessel and the centrifuge tube to each unit. All units being constructed for storage in a clean bench. The apparatus may include a clean bench having a sterile operation space, and the aforementioned units may be removably stored within the sterile operation space.
摘要翻译：细胞培养装置空间小，提供经济效益，减少工作劳动，错误操作，促进防止污染，适用于小规模细胞培养。该装置允许至少执行介质置换处理和通道培养处理，并且包括用于抽取液体的抽吸单元，用于供给介质的药液供应单元，用于分离的液体，洗涤液，移液单元用于定量抽吸和排出液体;离心机，用于将细胞从离心管中的细胞悬浮液中分离;以及处理单元，用于将培养容器和离心管移动到每个单元。所有单位正在建造中，用于存放在干净的工作台上。该设备可以包括具有无菌操作空间的清洁台，并且上述单元可以可移除地存储在无菌操作空间内。

5. 发明申请

US20110311617A1 ORAL VACCINE 有权
标题翻译：口腔疫苗
公开(公告)号：US20110311617A1
公开(公告)日：2011-12-22
申请号：US12531507
申请日：2008-03-19
申请人： Toshiro Shirakawa , Masato Kawabata , Tetsuo Takata , Michiko Taniguchi , Asako Okamoto , Masanori Asada , Masaaki Nakatsuji
发明人： Toshiro Shirakawa , Masato Kawabata , Tetsuo Takata , Michiko Taniguchi , Asako Okamoto , Masanori Asada , Masaaki Nakatsuji
IPC分类号： A61K9/48 , A61P37/04 , A61P31/04 , A61K39/112 , A61K39/106
CPC分类号： A61K9/4891 , A61K39/0275 , A61K39/0283 , A61K39/107 , A61K47/6901 , A61K2039/522 , A61K2039/523 , A61K2039/542 , Y02A50/472 , Y02A50/476 , Y02A50/482 , Y02A50/484
摘要： The present invention provides an oral vaccine against a bacterial infectious disease (e.g., typhoid fever, cholera, or dysentery). The oral vaccine of the present invention is a capsule formulation in which a transformed microorganism that expresses a flagellin antigen protein or that secretes a flagellin antigen protein out of the cell of the microorganism is encapsulated with an acid-resistant membrane. Examples of the microorganism include intestinal bacteria belonging to the genus Bifidobacterium, the genus Lactobacillus, the genus Lactococcus, and the like. The form of the capsule formulation may be any one of a seamless capsule formulation, a soft capsule formulation, and a hard capsule formulation.
摘要翻译：本发明提供了针对细菌感染性疾病（例如伤寒，霍乱或痢疾）的口服疫苗。本发明的口服疫苗是一种胶囊制剂，其中表达鞭毛蛋白抗原蛋白或将鞭毛蛋白抗原蛋白从微生物细胞分泌出来的转化微生物用耐酸膜包封。微生物的实例包括属于双歧杆菌属，乳杆菌属，乳球菌属等的肠细菌。胶囊制剂的形式可以是无缝胶囊制剂，软胶囊制剂和硬胶囊制剂中的任何一种。

6. 发明授权

US08758760B2 Oral vaccine 有权
标题翻译：口服疫苗
公开(公告)号：US08758760B2
公开(公告)日：2014-06-24
申请号：US12531507
申请日：2008-03-19
申请人： Toshiro Shirakawa , Masato Kawabata , Tetsuo Takata , Michiko Taniguchi , Asako Okamoto , Masanori Asada , Masaaki Nakatsuji
发明人： Toshiro Shirakawa , Masato Kawabata , Tetsuo Takata , Michiko Taniguchi , Asako Okamoto , Masanori Asada , Masaaki Nakatsuji
IPC分类号： A61K39/00 , A61K39/38 , C12N1/20
CPC分类号： A61K9/4891 , A61K39/0275 , A61K39/0283 , A61K39/107 , A61K47/6901 , A61K2039/522 , A61K2039/523 , A61K2039/542 , Y02A50/472 , Y02A50/476 , Y02A50/482 , Y02A50/484
摘要： The present invention provides an oral vaccine against a bacterial infectious disease (e.g., typhoid fever, cholera, or dysentery). The oral vaccine of the present invention is a capsule formulation in which a transformed microorganism that expresses a flagellin antigen protein or that secretes a flagellin antigen protein out of the cell of the microorganism is encapsulated with an acid-resistant membrane. Examples of the microorganism include intestinal bacteria belonging to the genus Bifidobacterium, the genus Lactobacillus, the genus Lactococcus, and the like. The form of the capsule formulation may be any one of a seamless capsule formulation, a soft capsule formulation, and a hard capsule formulation.
摘要翻译：本发明提供了针对细菌感染性疾病（例如伤寒，霍乱或痢疾）的口服疫苗。本发明的口服疫苗是一种胶囊制剂，其中表达鞭毛蛋白抗原蛋白或将鞭毛蛋白抗原蛋白从微生物细胞分泌出来的转化微生物用耐酸膜包封。微生物的实例包括属于双歧杆菌属，乳杆菌属，乳球菌属等的肠细菌。胶囊制剂的形式可以是无缝胶囊制剂，软胶囊制剂和硬胶囊制剂中的任何一种。

7. 发明申请

US20120177687A1 GENE EXPRESSING A BIFIDOBACTERIUM SURFACE-PRESENTED FUSION PROTEIN 有权
标题翻译：基因表达双歧杆菌表面融合蛋白
公开(公告)号：US20120177687A1
公开(公告)日：2012-07-12
申请号：US13124178
申请日：2010-09-17
申请人： Toshiro Shirakawa , Sakura Yamamoto , Takane Katayama , Jun Wada , Yasunobu Kano , Masanori Asada , Kosuke Shimamoto
发明人： Toshiro Shirakawa , Sakura Yamamoto , Takane Katayama , Jun Wada , Yasunobu Kano , Masanori Asada , Kosuke Shimamoto
IPC分类号： A61K39/02 , A61P31/04 , C12N1/21 , A61P37/04 , C07H21/00 , C12N15/63
CPC分类号： C12N15/1037 , A61K39/0275 , A61K39/385 , A61K2039/523 , A61K2039/542 , A61K2039/55516 , C12N15/746 , Y02A50/482
摘要： According to the present invention, there is provided a means of expressing and displaying a protein on the cell surface of a bifidobacterium. In the gene for expressing a protein on the surface of a bifidobacterium of the present invention, a gene coding for a bifidobacterium-derived GNB/LNB substrate-binding membrane protein and a gene coding for the target protein or peptide are linked in this order from the 5′ end side. Thus, a bifidobacterium transformed by introducing the gene for expressing a protein on the surface of a bifidobacterium of the present invention expresses the target protein or peptide on the surface thereof. When the target protein or peptide is an antigen protein or an antigen peptide, the transformed bifidobacterium of the present invention is useful as an oral vaccine.
摘要翻译：根据本发明，提供了在双歧杆菌的细胞表面上表达和显示蛋白质的方法。在本发明的双歧杆菌表面上表达蛋白质的基因中，编码双歧杆菌来源的GNB / LNB底物结合膜蛋白的基因和编码靶蛋白或肽的基因以从 5'端。因此，通过在本发明的双歧杆菌的表面引入用于表达蛋白质的基因而转化的双歧杆菌在其表面上表达靶蛋白质或肽。当靶蛋白或肽是抗原蛋白或抗原肽时，本发明的转化双歧杆菌可用作口服疫苗。

8. 发明授权

US08354113B2 Gene expressing a bifidobacterium surface-presented fusion protein 有权
标题翻译：表达双歧杆菌表达融合蛋白的基因
公开(公告)号：US08354113B2
公开(公告)日：2013-01-15
申请号：US13124178
申请日：2010-09-17
申请人： Toshiro Shirakawa , Sakura Yamamoto , Takane Katayama , Jun Wada , Yasunobu Kano , Masanori Asada , Kosuke Shimamoto
发明人： Toshiro Shirakawa , Sakura Yamamoto , Takane Katayama , Jun Wada , Yasunobu Kano , Masanori Asada , Kosuke Shimamoto
IPC分类号： A61K39/02 , A61K39/145 , A61K39/112 , A61K39/00 , A01N63/00 , C12N15/74 , C07H21/04
CPC分类号： C12N15/1037 , A61K39/0275 , A61K39/385 , A61K2039/523 , A61K2039/542 , A61K2039/55516 , C12N15/746 , Y02A50/482
摘要： According to the present invention, there is provided a means of expressing and displaying a protein on the cell surface of a bifidobacterium. In the gene for expressing a protein on the surface of a bifidobacterium of the present invention, a gene coding for a bifidobacterium-derived GNB/LNB substrate-binding membrane protein and a gene coding for the target protein or peptide are linked in this order from the 5′ end side. Thus, a bifidobacterium transformed by introducing the gene for expressing a protein on the surface of a bifidobacterium of the present invention expresses the target protein or peptide on the surface thereof. When the target protein or peptide is an antigen protein or an antigen peptide, the transformed bifidobacterium of the present invention is useful as an oral vaccine.
摘要翻译：根据本发明，提供了在双歧杆菌的细胞表面上表达和显示蛋白质的方法。在本发明的双歧杆菌表面上表达蛋白质的基因中，编码双歧杆菌来源的GNB / LNB底物结合膜蛋白的基因和编码靶蛋白或肽的基因以从 5'端。因此，通过在本发明的双歧杆菌的表面引入用于表达蛋白质的基因而转化的双歧杆菌在其表面上表达靶蛋白质或肽。当靶蛋白或肽是抗原蛋白或抗原肽时，本发明的转化双歧杆菌可用作口服疫苗。

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