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1. 发明授权

US07396676B2 Evanescent wave sensor with attached ligand 有权
标题翻译：具有附着配体的渐逝波传感器
公开(公告)号：US07396676B2
公开(公告)日：2008-07-08
申请号：US11143358
申请日：2005-05-31
申请人： Karla M. Robotti , Daniel B. Roitman
发明人： Karla M. Robotti , Daniel B. Roitman
IPC分类号： C12M1/34
CPC分类号： G01N21/553 , G01N33/54353 , Y10S435/808 , Y10S436/805
摘要： The invention in particular embodiments provides an evanescent wave sensor which includes a ligand bound to a sensor substrate via a NCYX linker moiety, as defined herein. Methods of making the subject evanescent wave sensors are also provided which include contacting a first reactive moiety which has an isocyanato (or isothiocyanato) moiety with a second reactive moiety which has an hydroxyl, thiol, or amino moiety, as further described herein. Also provided by the invention are methods in which a subject evanescent wave sensor is contacted with a sample, and binding of analytes in the sample to the sensor is assessed by evanescent wave detection. The invention also provides kits and systems for performing the subject methods.
摘要翻译：本发明在特定实施方案中提供了一种ev逝波传感器，其包括通过如本文所定义的NCYX接头部分与传感器底物结合的配体。还提供了制备对象ev逝波传感器的方法，其包括使具有异氰酸酯基（或异硫氰酸酯基）部分的第一反应性部分与具有羟基，硫醇或氨基部分的第二反应性部分接触，如本文进一步描述的。本发明还提供了一种方法，其中对象ev逝波传感器与样品接触，并且通过ev逝波检测来评估样品中的分析物与传感器的结合。本发明还提供用于执行主题方法的套件和系统。

2. 发明申请

US20080318334A1 Microfluidic devices comprising fluid flow paths having a monolithic chromatographic material 审中-公开
标题翻译：微流体装置包括具有整体色谱材料的流体流动路径
公开(公告)号：US20080318334A1
公开(公告)日：2008-12-25
申请号：US11820856
申请日：2007-06-20
申请人： Karla M. Robotti
发明人： Karla M. Robotti
IPC分类号： G01N30/02 , B01J19/00 , B23P17/04
CPC分类号： G01N30/6095 , G01N2030/528 , Y10T29/494 , Y10T156/10
摘要： Aspects of the invention include a microfluidic device having a fluid flow path and a monolithic chromatographic material covalently bound to at least a portion of a polymeric surface of the fluid flow path. Aspects of the invention further include methods of making and using such devices.
摘要翻译：本发明的方面包括具有流体流动路径和共价结合到流体流动路径的聚合物表面的至少一部分的整体色谱材料的微流体装置。本发明的方面还包括制造和使用这些装置的方法。

3. 发明授权

US06375901B1 Chemico-mechanical microvalve and devices comprising the same 有权
公开(公告)号：US06375901B1
公开(公告)日：2002-04-23
申请号：US09294867
申请日：1999-04-20
申请人： Karla M. Robotti , Sally A. Swedberg , Hongfeng Yin
发明人： Karla M. Robotti , Sally A. Swedberg , Hongfeng Yin
IPC分类号： B01L1100
CPC分类号： B81B3/0035 , B01J19/0093 , B01J2219/00891 , B01J2219/00907 , B01J2219/00909 , B01L3/502738 , B01L2300/0816 , B01L2300/087 , B01L2400/0677 , F16K99/0001 , F16K99/0017 , F16K99/0025 , F16K99/0036 , F16K99/0044 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/0084 , Y10T137/2082 , Y10T436/2575
摘要： Micro-fluid devices and methods for their use are provided. The subject devices are characterized by the presence of at least one micro-valve comprising a phase reversible material, e.g. a reversible gel, that reversibly changes its physical state in response to an applied stimulus, e.g. a thermoreversible gel. In using the subject device, fluid flow along a flow path of the device is modulated by applying an appropriate stimulus, e.g. changing the temperature, to the microvalve. The subject devices find use in a variety of applications, including micro-analytical applications.

4. 发明授权

US5384411A Immobilization of PH-sensitive dyes to solid supports 失效
标题翻译：将PH敏感染料固定在固体支持物上
公开(公告)号：US5384411A
公开(公告)日：1995-01-24
申请号：US718062
申请日：1991-06-20
申请人： Karla M. Robotti , Carl A. Myerholtz
发明人： Karla M. Robotti , Carl A. Myerholtz
IPC分类号： C07D307/77 , C07D307/88 , C07D307/885 , C07D307/92 , C07D327/04 , C09B11/06 , G01N31/22 , C07D307/78
CPC分类号： C07D307/885 , C07D307/92 , C07D327/04 , C09B11/06 , G01N31/221
摘要： Disclosed are pH dyes containing a suitable compatible substituent which permits binding of the dye to a solid support. Also disclosed are methods for synthesizing such dyes prior to their coupling to the solid supports.
摘要翻译：公开了含有合适的相容取代基的pH染料，其允许染料与固体支持物结合。还公开了在与固体支持物偶联之前合成这些染料的方法。

5. 发明授权

US07425451B2 Triazine derivatives as universal peptide isotope tag reagents (U-PIT) 失效
标题翻译：三嗪衍生物作为通用肽同位素标签试剂（U-PIT）
公开(公告)号：US07425451B2
公开(公告)日：2008-09-16
申请号：US10318845
申请日：2002-12-13
申请人： Karla M. Robotti , James Alexander Apffel, Jr.
发明人： Karla M. Robotti , James Alexander Apffel, Jr.
IPC分类号： G01N33/00 , C07D403/00 , A01N43/66 , A61K31/53
CPC分类号： C07D403/12 , C07D401/14
摘要： Compounds, compositions, methods for sequencing proteins and peptides, and methods for identifying proteins and peptides in a mixture, are disclosed. Compounds of formula A-B-C wherein A is a nucleophilic reactive group, B is a detectable moiety capable of being isotopically labeled, and C is a charge replacement group, are used to label the peptides at the N-terminus or the C-terminus. The tagged peptides can then be analyzed by mass spectroscopy.
摘要翻译：公开了用于测定蛋白质和肽的化合物，组合物，方法以及用于鉴定混合物中的蛋白质和肽的方法。其中A是亲核反应性基团的式A-B-C化合物，B是能够同位素标记的可检测部分，C是电荷取代基，用于在N末端或C末端标记肽。然后可以通过质谱法分析标记的肽。

6. 发明授权

US07201834B2 Methods and compositions for modifying the surface of a polymeric substrate 失效
标题翻译：用于改性聚合物基材表面的方法和组合物
公开(公告)号：US07201834B2
公开(公告)日：2007-04-10
申请号：US10386581
申请日：2003-03-11
申请人： Sally A. Swedberg , Karla M. Robotti , Karin Caldwell
发明人： Sally A. Swedberg , Karla M. Robotti , Karin Caldwell
IPC分类号： G01N27/453
CPC分类号： C08J7/047 , C08G65/329 , C08G65/33303 , C08G65/334 , C08J2471/00 , C09D171/02 , C25D13/22
摘要： Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of about 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.
摘要翻译：提供了改性聚合物基材表面的方法和组合物。在本方法中，聚合物基材的表面的至少一部分与生物结垢的表面活性剂组合物接触。组合物的表面活性剂分子的至少一部分用在约2至12的pH范围内电荷稳定的非反应性带电端基末端修饰。本发明方法和组合物特别用于电泳装置，例如作为毛细管电泳装置。

7. 发明授权

US5108502A Boronic acid dyes 失效
标题翻译：硼酸染料
公开(公告)号：US5108502A
公开(公告)日：1992-04-28
申请号：US751457
申请日：1991-08-28
申请人： Norman E. Pawlowski , Dale D. Russell , Karla M. Robotti
发明人： Norman E. Pawlowski , Dale D. Russell , Karla M. Robotti
IPC分类号： C09B29/01 , C09B31/02 , C09B31/057 , C09B31/072 , C09B31/16 , C09B31/30 , C09D11/00 , D06P5/00
CPC分类号： C09D11/328 , C09B29/0007 , C09B31/057 , C09B31/072
摘要： Boronic dyes corresponding to the formula ##STR1## wherein D is an organic dye moiety, and n is 1 or 2 are described. Inks which are formulated for ink-jet printing and contain such a dye provide excellent images when printed on paper.
摘要翻译：对应于式“IMAGE”的硼酸染料，其中D为有机染料部分，n为1或2。用于喷墨印刷并含有这种染料的油墨在纸上印刷时提供优异的图像。

8. 发明申请

US20090084166A1 Sensors comprising a chelated metal ion linker layer 审中-公开
标题翻译：包含螯合金属离子接头层的传感器
公开(公告)号：US20090084166A1
公开(公告)日：2009-04-02
申请号：US11904686
申请日：2007-09-27
申请人： Karla M. Robotti
发明人： Karla M. Robotti
IPC分类号： G01N33/487 , B01J19/00 , B05D1/36
CPC分类号： G01N33/48707
摘要： Embodiments of sensors according to the invention include a substrate having an activated dielectric surface; a metal ion chelate layer on the activated dielectric surface; and a bifunctional layer on a surface of said metal ion chelate layer, where the bifunctional layer includes bifunctional molecules that have a metal ion affinity moiety; and a ligand attachment moiety. Ligands are located at one or more positions on the surface of the bifunctional layer in certain embodiments. Aspects of the invention further include methods of making the sensors, as well as kits for practicing the methods. Further aspects of the invention include methods of using the sensors, e.g., in analyte detection applications.
摘要翻译：根据本发明的传感器的实施例包括具有活化的电介质表面的衬底; 在活化的电介质表面上的金属离子螯合层; 和在所述金属离子螯合层的表面上的双功能层，其中所述双功能层包括具有金属离子亲和部分的双功能分子; 和配体附着部分。在某些实施方案中，配体位于双功能层表面上的一个或多个位置。本发明的方面还包括制造传感器的方法以及用于实施该方法的试剂盒。本发明的其它方面包括使用传感器的方法，例如在分析物检测应用中。

9. 发明授权

US07109040B2 Modular isotope coding approach to proteomic analysis 有权
标题翻译：模块化同位素编码方法进行蛋白质组学分析
公开(公告)号：US07109040B2
公开(公告)日：2006-09-19
申请号：US10318475
申请日：2002-12-13
申请人： James Alexander Apffel, Jr. , Karla M. Robotti
发明人： James Alexander Apffel, Jr. , Karla M. Robotti
IPC分类号： G01N24/00 , C07K1/13 , C07K1/14 , C07K1/16
CPC分类号： G01N33/6803 , Y10T436/24
摘要： The present invention provides methods for analyzing a peptide or peptides of interest in a protein sample using a combination of a relatively generic isotope tag with a decoupled selection process, allowing simplified customization of the application with a single reagent. These methods comprise providing a first and a second protein sample; labeling the first protein sample with a first Universal Peptide Isotope Tag (U-PIT) reagent and the second protein sample with a second U-PIT reagent; separating the peptide of interest from the combined first and second protein samples; and determining the relative amount of the first U-PIT reagent and the second U-PIT reagent bound to the peptide or peptides of interest. The U-PIT label of the present inventive methods has the following general formula A-B-C wherein A is a nucleophilic reactive group, B is a detectable moiety that can be isotopically labeled, and C is a charge replacement group.
摘要翻译：本发明提供使用相对通用的同位素标签与解耦选择过程的组合来分析蛋白质样品中的目标肽或肽的方法，允许用单一试剂简化定制应用。这些方法包括提供第一和第二蛋白质样品; 用第一个通用肽同位素标签（U-PIT）试剂标记第一个蛋白质样品，第二个蛋白质样品用第二个U-PIT试剂标记; 从合并的第一和第二蛋白样品中分离目的肽; 并确定与感兴趣的肽或肽结合的第一U-PIT试剂和第二U-PIT试剂的相对量。本发明方法的U-PIT标签具有以下通式：在线公式描述=“在线公式”end =“lead”？> ABC <？in-line-formula description =“In- 其中A是亲核反应性基团，B是可以同位素标记的可检测部分，C是电荷置换基团。

10. 发明授权

US06783992B2 Methods and using chemico-mechanical microvalve devices for the selective separation of components from multi-component fluid samples 有权
标题翻译：方法和使用化学 - 机械微型阀装置，用于从多组分流体样品中选择性分离组分
公开(公告)号：US06783992B2
公开(公告)日：2004-08-31
申请号：US09754687
申请日：2001-01-03
申请人： Karla M. Robotti , Hongfeng Yin
发明人： Karla M. Robotti , Hongfeng Yin
IPC分类号： G01N118
CPC分类号： B01L3/502738 , B01L2300/0681 , B01L2400/0661 , B01L2400/0677 , G01N30/08 , G01N30/14 , G01N30/20 , Y10T436/25375 , Y10T436/255
摘要： Methods for selectively separating at least one component from a multi-component componant fluidic sample are provided. In the subject methods, the fluidic sample is introduced into a micro-fluidic device that includes at least one micro-valve made up of a phase reversible material. The multi-component fluidic sample is then contacted with the microvalve in a microfluidic device under conditions sufficient for the at least one component to enter the microvalve, while the remaining constituents of the fluidic sample remain outside of the microvalve. Also provided are kits for use in practicing the subject methods, where the kits include at least a microfluidic device having a microvalve and instructional material (or means for obtaining the same) on how to use the device in the subject methods. The subject devices find use in a variety of applications, including sample desalting and concentration applications.
摘要翻译：提供了从多成分组分流体样品中选择性分离至少一种组分的方法。在本发明的方法中，将流体样品引入微流体装置，其包括由相位可逆材料构成的至少一个微型阀。然后在足以使至少一种组分进入微型阀的条件下，多组分流体样品与微流体装置中的微型阀接触，而流体样品的剩余组分保留在微型阀的外部。还提供了用于实践主题方法的试剂盒，其中试剂盒包括至少一种具有微血管和微量元素的微流体装置（或用于获得本发明的方法的方法）。主题装置可用于各种应用，包括样品脱盐和浓缩应用。

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