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    • 2. 发明申请
    • Alkaline protease
    • 碱性蛋白酶
    • US20020064854A1
    • 2002-05-30
    • US09920954
    • 2001-08-03
    • KAO CORPORATION
    • Mikio TakaiwaMitsuyoshi OkudaKatsuhisa SaekiHiromi KubotaJun HitomiYasushi KageyamaShitsuw ShikataMasafumi Nomura
    • C12N009/52C12N001/21C07H021/04
    • C12N9/54C11D3/386C11D3/38609C11D3/38618
    • An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40null C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.
    • 具有以下特性的碱性蛋白酶; 编码该基因的基因; 生产该微生物的微生物; 和含有该组合物的洗涤组合物; (i)在4至13的广泛pH值范围内起作用,并且在最佳pH值下达到pH6至12,80%或更高的活性; (ii)当在40℃下处理30分钟时,在6至11的pH值范围内是稳定的; (iii)具有约8.9至9.1的等电点; 和(iv)具有不被油酸抑制的酪蛋白消化活性。 本发明的碱性蛋白酶对各种表面活性剂和脂肪酸高度稳定,对氧化剂表现出高稳定性,因此可用作自动洗碗机和洗衣洗涤剂的洗涤剂中的酶,两者均含有漂白组分 。