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1. 发明申请

US20040203129A1 Alkaline proteases 有权
公开(公告)号：US20040203129A1
公开(公告)日：2004-10-14
申请号：US10837566
申请日：2004-05-04
申请人： KAO CORPORATION
发明人： Yuji Hatada , Akinori Ogawa , Yasushi Kageyama , Tsuyoshi Sato , Hiroyuki Araki , Nobuyuki Sumitomo , Mitsuyoshi Okuda , Katsuhisa Saeki
IPC分类号： C12N009/54 , C12N009/56
CPC分类号： C12N9/54 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to alkaline proteases having high specific activity and strong oxidant resistance. The present invention also relates to alkaline proteases having excellent detergency that is to be added to a detergent.

2. 发明申请

US20030215906A1 Recombinant bacillus proteases and uses thereof 失效
标题翻译：重组杆菌蛋白酶及其用途
公开(公告)号：US20030215906A1
公开(公告)日：2003-11-20
申请号：US10146905
申请日：2002-05-17
发明人： Boon Leong Lim
IPC分类号： C12P021/02 , C12N009/08 , C12N009/16 , C12N009/24 , C12N009/56 , C12N001/21 , C12N015/74
CPC分类号： C12N9/54 , C12N15/75
摘要： The present invention provides genetically engineered Bacillus strains that can secrete large amount of Bacillus proteases in the extracellular culture medium. More particularly, this invention relates to a process of producing recombinant protease molecules of Bacillus origin in a Bacillus subtilis strain 168, utilizing a strong prophage promoter.
摘要翻译：本发明提供了可在细胞外培养基中分泌大量芽孢杆菌蛋白酶的遗传工程芽孢杆菌菌株。更具体地说，本发明涉及利用强前体启动子在枯草芽孢杆菌菌株168中产生芽孢杆菌来源的重组蛋白酶分子的方法。

3. 发明申请

US20020155575A1 Subtilase variants 失效
标题翻译： Subtilase变体
公开(公告)号：US20020155575A1
公开(公告)日：2002-10-24
申请号：US09976414
申请日：2001-10-12
申请人： Novozymes A/S
发明人： Mads Norregaard-Madsen , Line Bloch Larsen , Peter Kamp Hansen
IPC分类号： C12N009/56 , D06L001/00 , C07H021/04 , C12P021/02 , C12N001/21
CPC分类号： C12N9/54 , C11D3/38681
摘要： The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42-43, 51-56, 155-161, 187-190, 216-217, 217-218 or 218-219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.
摘要翻译：本发明涉及具有减少鸡蛋中存在的物质的抑制倾向的减毒酶变体，例如IV-0型胰蛋白酶抑制剂。特别地，所述变体包含位置42-43,51-56,155-161,187-190,216-217,217-218或218-219之间的至少一个另外的氨基酸残基（以BASBPN编号）。这些枯草芽孢杆菌变体在用于例如蛋白质时在蛋渍上表现出优异或改善的洗涤性能是有用的。清洁剂或洗涤剂组合物，例如衣物洗涤剂组合物和洗涤剂组合物，包括自动洗碗剂组合物。而且，编码变体，分离的载体，宿主细胞的分离的DNA序列和用于产生和使用本发明的变体的方法。此外，公开了包含变体的清洁剂和洗涤剂组合物。

4. 发明申请

US20040002432A1 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US20040002432A1
公开(公告)日：2004-01-01
申请号：US10385662
申请日：2003-03-12
申请人： KAO CORPORATION
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
IPC分类号： C11D003/386 , C07H021/04 , C12N009/56 , C12P021/02 , C12N001/21 , C12N015/74
CPC分类号： C11D3/386 , C12N9/54 , C12Y304/21062
摘要： Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine. The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译：在本发明中提供的是碱性蛋白酶，其中（a）位置65，（b）位置101，（c）位置163，（d）位置170，（e）位置171，（f），（g）位置320，（h）位置359或（i）SEQ ID NO：位置（a）：脯氨酸，位置（b）：天冬酰胺，位置（c）：组氨酸，天冬氨酸，苯丙氨酸，赖氨酸，天冬酰胺，位置（d）：缬氨酸和亮氨酸，位置（e）：丙氨酸，谷氨酸，甘氨酸和苏氨酸，位置（f）：异亮氨酸，甘氨酸和苏氨酸，位置（g）位置（h）：丝氨酸，亮氨酸，缬氨酸，异亮氨酸和谷氨酰胺，位置（i）：丙氨酸，赖氨酸，谷氨酰胺，谷氨酸，精氨酸和组氨酸。本发明使得可以有效地生产和提供具有活性的碱性蛋白酶，即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质，皮脂等的复合物染色剂，因此可用作要掺入洗涤剂中的酶。

5. 发明申请

US20030191039A1 Modified subtilisins and detergent, compositions containing same 失效
标题翻译：改性枯草杆菌蛋白酶和洗涤剂，含有它们的组合物
公开(公告)号：US20030191039A1
公开(公告)日：2003-10-09
申请号：US10314191
申请日：2002-12-06
申请人： Novozymes A/S
发明人： Dorrit Aaslyng , Sven Branner , Sven Hastrup , Leif Norskov-Lauritsen , Ole Hvilsted , Merete Simonsen , Eric Casteleijn , Maarten Robert Egmond , Johan Haverkamp , John David Marugg , Arnoldus Theodorus Anthonius Mooren
IPC分类号： C11D003/386 , C07H021/04 , C12N009/56 , C12P021/02 , C12N001/21 , C12N015/74
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38636
摘要： The present invention relates to enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.
摘要翻译：本发明涉及通过突变多种枯草杆菌蛋白酶的基因并在合适的宿主中表达突变的基因而产生的酶。与其野生型亲本酶相比，酶显示改善的洗涤性能。酶非常适合用于洗涤剂组合物中。

6. 发明申请

US20030186378A1 Useful mutations of bacterial alkaline protease 失效
公开(公告)号：US20030186378A1
公开(公告)日：2003-10-02
申请号：US10310730
申请日：2002-12-05
申请人： Novozymes A/S
发明人： Sven Hastrup , Sven Branner , Fanny Norris , Steffen Bjorn Petersen , Leif Norskov-Lauridsen , Villy Johannes Jensen , Dorrit Aaslyng
IPC分类号： C12P021/02 , C12N001/21 , C07H021/04 , C12N009/56 , C12N015/74
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.

7. 发明申请

US20030180932A1 Proteases from gram-positive organisms 失效
公开(公告)号：US20030180932A1
公开(公告)日：2003-09-25
申请号：US10401436
申请日：2003-03-26
发明人： David A. Estell
IPC分类号： C12N009/54 , C12N009/56 , C07H021/04 , C12P021/02 , C12N001/21 , C07K014/52 , C12N015/74
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38663 , C12N9/18 , C12N9/48 , C12N15/75
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.

8. 发明申请

US20030171235A1 Subtilase enzymes 有权
标题翻译：分泌酶
公开(公告)号：US20030171235A1
公开(公告)日：2003-09-11
申请号：US10242549
申请日：2002-09-12
申请人： Novozymes A/S
发明人： Peter Kamp Hansen , Peter Bauditz , Frank Mikkelsen , Kim Vilbour Andersen , Carsten Andersen , Mads Norregaard-Madsen
IPC分类号： C11D003/386 , C12N009/56 , C12N015/74 , C12P021/02 , C12N001/21 , C07H021/04
CPC分类号： C12N9/54 , C11D3/38681 , C12Y304/21062
摘要： The present invention relates to subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid in the active site loop (b) region from positions 95 to 103. The variant subtilases of the present invention exhibit improved wash performance in a detergent in comparison to its parent enzyme.
摘要翻译：本发明涉及在位置95至103的活性位点环（b）区域中具有另外的氨基酸的I-S1和I-S2亚基的脱羧酶。本发明的变体消除酶表现出改进的洗涤性能在洗涤剂中与其母体酶相比。

9. 发明申请

US20040063177A1 Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins 审中-公开
标题翻译：人类蛋白酶和这种蛋白酶用于药物应用和降低非人蛋白质的过敏原性
公开(公告)号：US20040063177A1
公开(公告)日：2004-04-01
申请号：US10665689
申请日：2003-09-18
发明人： David A. Estell
IPC分类号： A61K038/48 , C12N009/56 , C07H021/04 , C12P021/02 , C12N005/06
CPC分类号： C12N9/6424 , A61K38/00 , C12N9/54
摘要： The present invention relates to a method of producing novel improved protein mutant which produce low allergenic response in humans compared to the parent of that mutant. Specifically, the present invention comprises neutralizing or reducing the allergenicity of a protein by introducing therein as replacement or modification of an epitope on such protein a sequence from human subtilisin.
摘要翻译：本发明涉及一种与该突变体的亲本相比，在人类中产生低变应原反应的新型改良蛋白质突变体的方法。具体地说，本发明包括通过引入蛋白质来中和或降低蛋白质的变应原性，作为来自人枯草杆菌蛋白酶的这种蛋白质上的表位的替代或修饰。

10. 发明申请

US20030176304A1 Protease variants and compositions 失效
标题翻译：蛋白酶变体和组成
公开(公告)号：US20030176304A1
公开(公告)日：2003-09-18
申请号：US10336324
申请日：2003-01-03
申请人： Novozymes A/S
发明人： Peter Kamp Hansen , Peter Bauditz , Frank Mikkelsen , Kim Vilbour Andersen
IPC分类号： C12N009/56 , C07H021/04 , C12P021/02 , C12N001/21 , C11D003/386 , C12N015/74
CPC分类号： C11D3/386 , C12N9/54
摘要： The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.
摘要翻译：本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。与其野生型亲本酶相比，酶在任何洗涤剂中表现出改善的洗涤性能。

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