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1. 发明申请

US20060134197A1 Method of reducing unpleasant taste and/or unpleasant odor 审中-公开
标题翻译：减少不愉快味道和/或不愉快气味的方法
公开(公告)号：US20060134197A1
公开(公告)日：2006-06-22
申请号：US10540772
申请日：2003-12-24
申请人： Yukio Uchida , Kanou Takeuchi , Michio Kubota , Toshio Miyake
发明人： Yukio Uchida , Kanou Takeuchi , Michio Kubota , Toshio Miyake
IPC分类号： A61K9/20 , A23G3/00
CPC分类号： A23L27/33 , A23L5/27 , A23V2002/00 , A23V2200/16 , A23V2250/636
摘要： The present invention has objects to provide a method of lowering the unpleasant taste and/or smell of compositions, and an agent for lowering unpleasant taste and/or smell. The objects are solved by providing a method of lowering unpleasant taste and/or smell, comprising a step of incorporating an saccharide derivative of α,α-trehalose in compositions; a composition with lowered unpleasant taste and/or smell, obtainable by the method; and an agent for lowering unpleasant taste and/or smell, comprising an saccharide derivatives of α,α-trehalose as an effective ingredients.
摘要翻译：本发明的目的是提供降低组合物的不愉快味道和/或气味的方法，以及降低不愉快味道和/或气味的药剂。通过提供降低不愉快味道和/或气味的方法来解决目的，包括将α，α-海藻糖的糖衍生物掺入组合物中的步骤; 通过该方法获得的具有降低的不愉快味道和/或气味的组合物; 以及降低不愉快味道和/或气味的药剂，其包含α，α-海藻糖的糖衍生物作为有效成分。

2. 发明申请

US20060172059A1 Method of inhibiting water content variation of composition and use thereof 审中-公开
标题翻译：抑制组合物含水量变化的方法及其用途
公开(公告)号：US20060172059A1
公开(公告)日：2006-08-03
申请号：US10539138
申请日：2003-12-15
申请人： Kanou Takeuchi , Michio Kubota , Toshio Miyake
发明人： Kanou Takeuchi , Michio Kubota , Toshio Miyake
IPC分类号： A23G3/00 , A01N63/00
CPC分类号： A23K30/00 , A21D2/181 , A21D13/04 , A21D13/047 , A21D13/40 , A21D13/50 , A23B4/20 , A23B7/022 , A23C9/152 , A23C9/1544 , A23C20/025 , A23D9/05 , A23D9/06 , A23F3/14 , A23F3/163 , A23G3/00 , A23G3/346 , A23G3/42 , A23G9/34 , A23G2200/06 , A23K20/163 , A23L2/39 , A23L3/3427 , A23L3/3562 , A23L5/00 , A23L7/00 , A23L7/111 , A23L7/196 , A23L7/1965 , A23L9/00 , A23L9/12 , A23L9/22 , A23L11/05 , A23L15/30 , A23L19/03 , A23L19/105 , A23L21/00 , A23L21/12 , A23L25/25 , A23L27/16 , A23L27/33 , A23L27/50 , A23L27/60 , A23L27/70 , A23L29/274 , A23L29/30 , A23P20/10 , A23P30/40 , A23V2002/00 , A61K8/60 , A61K9/0048 , A61K9/4816 , A61K47/26 , A61K2800/52 , A61Q5/02 , A61Q5/12 , A61Q7/00 , A61Q19/00 , A61Q19/10 , C09K15/06 , C11B5/0021 , C11D3/221 , A23V2250/636
摘要： The present invention has objects for providing a method for inhibiting the moisture variation in compositions, a composition whose moisture variation is inhibited, and an agent for inhibiting the moisture variation in compositions. The objects are solved by providing a method for inhibiting the moisture variation in compositions comprising incorporating into a composition a saccharide-derivative(s) of α,α-trehalose as an effective ingredient, a composition whose moisture variation is inhibited by incorporating the saccharide-derivative(s), a moisture variation inhibiting agent containing the saccharide-derivative(s) as an effective ingredient, and uses thereof.
摘要翻译：本发明的目的在于提供抑制组合物中的水分变化的方法，抑制水分变化的组合物和抑制组合物中水分变化的试剂。通过提供一种抑制组合物中水分变化的方法来解决目的，包括将α，α-海藻糖的糖衍生物作为有效成分掺入到组合物中，通过掺入糖 - 衍生物，含有糖衍生物作为有效成分的防湿变化抑制剂及其用途。

3. 发明授权

US08759030B2 Process for producing 2-O-alpha-D-glucopyranosyl-L-ascorbic acid 有权
标题翻译：制备2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的方法
公开(公告)号：US08759030B2
公开(公告)日：2014-06-24
申请号：US10523920
申请日：2003-07-07
申请人： Kazuhisa Mukai , Keiji Tsusaki , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Keiji Tsusaki , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/44
CPC分类号： C12P19/60
摘要： The object of the present invention is to provide a method and a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid where 5-O-α-glucopyranosyl-L-ascorbic acid and 6-O-α-glucopyranosyl-L-ascorbic acid are not formed or formed in such a small amount that the formation of these can nor be detected. The present invention solves the above object by providing a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid comprising the steps of allowing α-isomaltosyl glucosaccharide-forming enzyme together with or without cyclomaltodextrin glucanotransferase (EC 2.4.1.19) to act on a solution comprising L-ascorbic acid and, an α-glucosyl saccharide to form 2-O-α-glucopyranosyl-L-ascorbic acid and collecting the formed 2-O-α-glucopyranosyl-L-ascorbic acid.
摘要翻译：本发明的目的是提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法和方法，其中5-O-α-吡喃葡萄糖基-L-抗坏血酸和6-O-α-吡喃葡萄糖基 - L-抗坏血酸不是以这样的少量形成或形成，也可以不形成。本发明通过提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法来解决上述目的，其包括以下步骤：将α-异麦芽糖基葡萄糖形成酶与或不与环糊精葡聚糖转移酶（EC 2.4.1.19）一起作用于包含L-抗坏血酸和α-葡萄糖基糖的溶液中以形成2-O-α-吡喃葡萄糖基-L-抗坏血酸并收集形成的2-O-α-吡喃葡萄糖基-L-抗坏血酸。

4. 发明申请

US20080027027A1 Lactosucrose High Content Saccharide, Its Preparation and Uses 审中-公开
标题翻译：乳糖蔗糖高含量糖，其制备和用途
公开(公告)号：US20080027027A1
公开(公告)日：2008-01-31
申请号：US11587248
申请日：2005-04-20
申请人： Hiroyuki Okabe , Hajime Aga , Michio Kubota , Toshio Miyake
发明人： Hiroyuki Okabe , Hajime Aga , Michio Kubota , Toshio Miyake
IPC分类号： A61K31/702 , A23L1/236 , A61K8/72 , A61P1/00 , A61Q99/00 , C12P19/00 , C13D3/16 , C13F1/02 , C13F3/00
CPC分类号： C13B30/02 , A23L27/33 , C07H3/06 , C12P19/14 , C12P19/18 , C13B20/142
摘要： An object of the present invention is to provide a lactosucrose high content saccharide which comprises 70% or more of lactosucrose, on a saccharide composition basis, with a lower content of 1-kestose and fructosyl lactosucrose as by-products. Another object of the present invention is to provide processes for producing a lactosucrose high content saccharide and high purity lactosucrose containing 90% or more of lactosucrose, on a saccharide composition basis, which are feasible for industrial production. Further object of the present invention is to provide a solid lactosucrose with low hygroscopicity and a solid composition comprising the same. The present invention solves above objects by providing a lactosucrose high content saccharide comprising 70% or more of lactosucrose and less than 3% of the total amount of 1-kestose and fructosyl lactosucrose, on a saccharide composition basis; a process for producing the lactosucrose high content saccharide, comprising the steps of allowing β-fructofuranosidase, derived from a microorganism belonging to the genus Bacillus, and sucrose-unassimilable yeast to contact with an aqueous solution containing sucrose and lactose to obtain a reaction mixture comprising 70% or more of lactosucrose and less than 3% of the total amount of 1-kestose and fructosyl lactosucrose and collecting the resulting lactosucrose high content saccharide; a process for producing a high purity lactosucrose, comprising the steps of subjecting the lactosucrose high content saccharide to a chromatography using a resin and collecting fractions containing 90% or more of lactosucrose, on a saccharide composition basis; a process for producing crystalline lactosucrose; and various solid compositions prepared by incorporating the crystalline lactosucrose.
摘要翻译：本发明的目的是提供一种乳糖蔗糖高含量糖，其以糖组成为基础含有70％以上的乳糖蔗糖，其中1-kestose和果糖基乳糖为副产物含量较低。本发明的另一个目的是提供一种以糖组成为基础制备含有90％以上乳蔗糖的乳糖蔗糖高含量糖和高纯度乳糖蔗糖的方法，其可用于工业生产。本发明的另一个目的是提供具有低吸湿性的固体乳糖蔗糖和包含它的固体组合物。本发明通过以糖组成为基础，提供含有70％以上的乳糖和低于1-kestose和果糖乳糖蔗糖总量的3％的乳糖蔗糖高含量糖，一种生产乳糖蔗糖高含量糖的方法，包括以下步骤：将衍生自属于芽孢杆菌属的微生物的β-呋喃果糖苷酶和蔗糖不可同化的酵母与含有蔗糖和乳糖的水溶液接触，得到包含 70％以上的乳蔗糖，少于1-kestose和果糖乳糖蔗糖总量的3％，并收集得到的乳糖蔗糖高含量糖; 一种生产高纯度乳糖蔗糖的方法，包括以糖组成为基础，使用树脂对乳糖蔗糖高含量糖进行色谱分离并收集含有90％以上乳蔗糖的级分的步骤; 一种生产结晶乳糖的方法; 和通过掺入结晶乳蔗糖制备的各种固体组合物。

5. 发明申请

US20080003651A1 alpha-isomaltosylglucosaccharide-forming enzyme, process and uses of the same 有权
标题翻译： α-异麦芽糖基葡萄糖形成酶，其工艺和用途
公开(公告)号：US20080003651A1
公开(公告)日：2008-01-03
申请号：US11797932
申请日：2007-05-09
申请人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04
CPC分类号： A23G1/40 , A21D2/181 , A23C9/005 , A23C9/1307 , A23C9/1544 , A23C9/156 , A23G3/42 , A23G4/10 , A23G9/34 , A23L2/60 , A23L15/00 , A23L15/20 , A23L27/30 , A23L33/10 , A23L33/21 , A61K8/60 , A61K8/66 , A61K9/0014 , A61K47/26 , A61Q11/00 , A61Q19/00 , A61Q19/02 , A61Q19/10 , C07H3/04 , C07H3/06 , C12N9/1051 , C12N9/2425 , C12N9/2428 , C12P19/04 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(166)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(16} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.
摘要翻译：本发明的目的是提供一种α-异麦芽糖基葡萄糖形成酶，其工艺，环四糖和包含可通过使用酶获得的糖的糖组合物; 并且通过建立形成葡糖聚合度至少为3并且在非还原末端具有α-1,6糖苷键作为连接的α糖异构体糖结合酶和α - 通过催化从葡萄糖聚合度至少为2并具有α-1,4糖苷键作为连接的糖的α-葡糖基转移而在非还原性末端的连接以外的1,4-葡糖苷键所述非还原端基本上不增加所述还原能力; 使用该酶的α-异麦芽糖基转移法; 形成α-异麦芽糖基糖的方法; 制备具有环{66）-alpha-D-吡喃葡萄糖基 - （163）-al-D-glucopyranosyl-（166）-al-D-glucopyranosyl-（163）-al-D-glucopyranosyl-（163）-al-D-glucopyranosyl- （16）同时使用α-异麦芽糖基糖产生酶和α-异麦芽糖基转移酶;以及可用其获得的糖的用途。

6. 发明申请

US20070154996A1 Method for transferring a glucosyl residue 审中-公开
标题翻译：转移葡糖基残基的方法
公开(公告)号：US20070154996A1
公开(公告)日：2007-07-05
申请号：US10587711
申请日：2005-01-27
申请人： Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04
CPC分类号： C12P19/18 , C12P19/44
摘要： An object of the present invention is to provide novel methods for forming glucosyl-transferred polyalcohols, glucosyl-transferred glucuronic acid, and glucosyl-transferred derivatives of glucose whose C-6 hydroxyl group bound to a saccharide by using an enzymatic reaction. The present invention solves the above object by providing a method for transferring a glucosyl residue to polyalcohols, glucuronic acid and/or derivatives of glucose whose C-6 hydroxyl group bound to a saccharide, comprising a step of: allowing a trehalose phosphorylase to act on a saccharide containing glucose as a component sugar and one or more polyalcohols selected from the group consisting of inositol, ribitol, erythritol, and glycerol; glucuronic acid and/or a salt thereof; and/or one or more derivatives of glucose whose C-6 hydroxyl group bound to a saccharide selected from the group consisting of isomaltose, gentiobiose, melibiose, isomaltotriose, and isopanose.
摘要翻译：本发明的目的是提供通过酶促反应形成C-6羟基与糖结合的葡萄糖转移的多元醇，葡糖基转移的葡萄糖醛酸和葡萄糖转移的葡萄糖转移衍生物的新方法。本发明通过提供一种将葡糖基残基转移到多糖，葡糖醛酸和/或其C-6羟基与糖结合的葡萄糖的衍生物的方法，包括以下步骤：使海藻糖磷酸化酶作用于含有葡萄糖作为组分糖和一种或多种选自肌醇，核糖醇，赤藓糖醇和甘油的多元醇的糖; 葡萄糖醛酸和/或其盐; 和/或一种或多种C-6羟基结合于选自异麦芽糖，龙胆糖，蜜二糖，异麦芽三糖和异丙糖的糖的葡萄糖衍生物。

7. 发明授权

US07192746B2 α-Isomaltosyltransferase, process for producing the same and use thereof 有权
标题翻译： α-异麦芽糖基转移酶，其制备方法和用途
公开(公告)号：US07192746B2
公开(公告)日：2007-03-20
申请号：US10296153
申请日：2001-05-22
申请人： Michio Kubota , Tomoyuki Nishimoto , Hajime Aga , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Hajime Aga , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/18 , C12N9/10 , C12N1/20
CPC分类号： A23G1/423 , A21D2/181 , A23C9/005 , A23C9/1307 , A23G1/40 , A23G1/56 , A23G3/346 , A23G4/10 , A23G2200/06 , A23L2/60 , A23L9/12 , A23L11/07 , A23L13/428 , A23L15/30 , A23L19/01 , A23L27/33 , A23L29/35 , A61K8/60 , A61K8/66 , A61Q11/00 , A61Q15/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , A61Q19/08 , A61Q19/10 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： The object of the present invention is to provide an α-isomaltosyl-transferring enzyme which forms a cyclotetrasaccharide having the structure of cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→} from a saccharide having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end; microorganisms which produce the enzyme; process for producing the enzyme; cyclotetrasaccharide or saccharide compositions comprising the same; and uses thereof.
摘要翻译：本发明的目的是提供一种α-异麦芽糖基转移酶，其形成具有环{ - > 6）-al-D-glucopyranosyl-（1-> 3）-al-D-glucopyranosyl- （1-> 6）-α-D-吡喃葡萄糖基 - （1-> 3）-alpha-D-吡喃葡萄糖基 - （1->}，其葡萄糖聚合度至少为3，并具有α-1 ，6个糖苷键作为非还原末端的连接键和除非还原末端之间的连接以外的α-1,4糖苷键;产生该酶的微生物;产生该酶的方法;环四糖或糖组合物，其包含相同;及其用途。

8. 发明授权

US06140487A DNA encoding kojibiose phosphorylase obtainable from thermoanaerobium, ' its preparation and uses 有权
标题翻译：编码由热酵母获得的曲霉糖磷酸化酶的DNA，其制备和用途
公开(公告)号：US06140487A
公开(公告)日：2000-10-31
申请号：US188404
申请日：1998-11-10
申请人： Tomoyuki Nishimoto , Michio Kubota , Hiroto Chaen , Toshio Miyake
发明人： Tomoyuki Nishimoto , Michio Kubota , Hiroto Chaen , Toshio Miyake
IPC分类号： A61K8/60 , A61K38/00 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C12N1/21 , C12N9/10 , C12N15/54 , C12P19/18 , C07H21/04 , C12N9/12
CPC分类号： A61K8/60 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C12N9/1051 , C12P19/18 , A61K38/00
摘要： A kojibiose phosphorylase which hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid, forms kojibiose and an inorganic phosphoric acid from .beta.-D-glucose-1-phosphoric acid, and catalyzes the transfer reaction of glucosyl group to other saccharides using .beta.-D-glucose-1-phosphoric acid as a saccharide donor. The enzyme is obtainable from natural sources such as microorganisms of the genus Thermoanaerobium, and obtainable by recombinant technology.
摘要翻译：在无机磷酸存在下水解曲霉糖以形成D-葡萄糖和β-D-葡萄糖-1-磷酸的曲霉糖磷酸化酶，从β-D-葡萄糖-1-磷酸形成曲酸和无机磷酸，并使用β-D-葡萄糖-1-磷酸作为糖供体催化葡糖基转移到其他糖类上。该酶可从天然来源例如Thermoanaerobium属的微生物获得，可通过重组技术获得。

9. 发明授权

US5922580A Non-reducing saccharide-forming enzyme, its preparation and uses 失效
公开(公告)号：US5922580A
公开(公告)日：1999-07-13
申请号：US941553
申请日：1997-09-30
申请人： Kazuhiko Maruta , Michio Kubota , Toshiyuki Sugimoto , Toshio Miyake
发明人： Kazuhiko Maruta , Michio Kubota , Toshiyuki Sugimoto , Toshio Miyake
IPC分类号： A23L1/236 , A21D2/18 , A23C9/13 , A23C9/152 , A23G3/00 , A23G3/34 , A23G3/42 , A23G3/48 , A23G4/00 , A23G4/10 , A23J3/16 , A23L1/09 , A23L1/31 , A23L1/314 , A23L1/32 , A23L2/60 , A61K8/00 , A61K8/60 , A61K8/66 , A61K8/73 , A61K9/36 , A61K31/00 , A61K31/02 , A61K38/21 , A61K47/26 , A61Q11/00 , A61Q17/04 , A61Q19/00 , C07H20060101 , C07H1/00 , C07H1/06 , C07H3/00 , C07H3/04 , C07H3/06 , C07K7/00 , C08B37/00 , C12N1/20 , C12N9/24 , C12N9/26 , C12N9/90 , C12P19/00 , C12P19/12 , C12P19/14 , C12P19/24 , C12R1/41 , C13K20060101 , C13K13/00 , C07H3/02
CPC分类号： C13K13/00 , A23G3/343 , A23G3/346 , A23G3/42 , A23G4/10 , A23L13/428 , A23L15/20 , A23L27/33 , A61K8/60 , A61K8/66 , A61Q11/00 , A61Q19/00 , C07H1/00 , C07H3/04 , C12N9/90 , C12P19/12 , A23G2200/06 , A61Q17/04 , A61Q19/02 , A61Q7/00
摘要： Disclosed are novel non-reducing saccharide-forming enzyme, and its preparation and uses. The enzyme is obtainable from the culture of microorganisms such as Rhizobium sp. M-11 (FERM BP 4130) and Arthrobacter sp. Q36 (FERM BP-4316), and capable of forming non-reducing saccharides having a trehalose structure when allowed to act on reducing partial starch hydrolysates. Glucoamylase and .alpha.-glucosidase readily yield trehalose when allowed to act on the non-reducing saccharides. These non-reducing saccharides and trehalose are extensively useful in food products, cosmetics and pharmaceuticals.

10. 发明授权

US5876975A Trehalose phosphorylase its preparation and uses 失效
标题翻译：海藻糖磷酸酶的制备及用途
公开(公告)号：US5876975A
公开(公告)日：1999-03-02
申请号：US103509
申请日：1998-06-24
申请人： Tetsuya Nakada , Michio Kubota , Hiroto Chaen , Toshio Miyake
发明人： Tetsuya Nakada , Michio Kubota , Hiroto Chaen , Toshio Miyake
IPC分类号： A61K38/00 , C12N1/21 , C12N9/10 , C12N15/54 , C12P19/18 , C12P19/44 , C07K1/00 , C12P21/06
CPC分类号： C12N9/1051 , C12P19/18 , A61K38/00
摘要： A thermostable trehalose phosphorylase which is obtainable from microorganisms of the genus Thermoanaerobium and which hydrolyzes trehalose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid. The trehalose phosphorylase can be also prepared by recombinant DNA technology. When the enzyme is allowed to contact with .beta.-D-glucose-1-phosphoric acid as a saccharide donor in the presence of other saccharides, glucosyl-transferred saccharides including glucosyl-D-galactoside, which are conventionally known but scarcely obtainable, can be produced on an industrial-scale and in a relatively-low cost.
摘要翻译：一种热稳定的海藻糖磷酸化酶，其可从Thermoanaerobium属的微生物获得，并且在无机磷酸存在下水解海藻糖以形成D-葡萄糖和β-D-葡萄糖-1-磷酸。海藻糖磷酸化酶也可以通过重组DNA技术制备。当酶在其他糖类存在下与β-D-葡萄糖-1-磷酸作为糖供体接触时，通常已知但几乎不可获得的包括葡糖基-D-半乳糖苷的葡糖基转移的糖可以是以工业规模生产，成本相对较低。

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