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1. 发明专利

JPH09220086A BREAD YEAST 失效
公开(公告)号：JPH09220086A
公开(公告)日：1997-08-26
申请号：JP2950996
申请日：1996-02-16
申请人： KYOWA HAKKO KOGYO KK
发明人： OUCHI KOZO , FUJIO TATSURO , IMURA TOSHIAKI , AZUMA MASAYUKI , KIKUCHI YASUHIRO , KAWASAKI HIDENORI
IPC分类号： A21D8/04 , C12N1/18 , C12R1/865
摘要： PROBLEM TO BE SOLVED: To obtain a bread yeast excellent in freezing resistance and refrigerating resistance and useful for a bread production method using frozen doughs or a bread production method using refrigerated doughs by changing a freezing or refrigerating-resistant yeast belonging to the genus Saccharomyces into a triploid or higher and specifying the precipitation rate, water content and molding amount of the changed yeast. SOLUTION: A freezing or refrigerating-resistant triploid or tetraploid yeast, Saccharomyces cerevisiae, having a precipitating speed of >=65 (preferably 65-100), a water content of =0.98kg/h (preferably 0.9-1.5kg/h) is selected from strains separated from the natural world, strains stored in research institutes, etc., fused cell strains, hybridized strains, etc. Saccharomyces cerevisiae FT3-1 (FERM BP-5397), FT4-1 (FERM BP-5398), etc., are exemplified.

2. 发明专利

JPH0445797A PRODUCTION OF L-ALPHA-ALANINE 失效
公开(公告)号：JPH0445797A
公开(公告)日：1992-02-14
申请号：JP15423190
申请日：1990-06-13
申请人： KYOWA HAKKO KOGYO KK
发明人： TAKADA HIDEYOSHI , OZAKI AKIO , KAWASAKI HIDENORI , HASHIMOTO YUKIO , AZUMA MASAYUKI , KAWAMOTO ISAO , OCHIAI KEIKO
IPC分类号： C12P41/00 , C12R1/01
摘要： PURPOSE:To efficiently obtain in high optical purity at a low cost the title compound useful as an intermediate for synthesizing medicines etc. by optically selective hydrolysis in an aqueous medium of L-alpha-alanineamide in the presence of the microbial cells of specific microorganisms belonging to Rahnella. CONSTITUTION:In the presence of the cultured product, microbial cells or treated product thereof of microorganisms belonging to Rahnella and capable of making asymmetric hydrolysis of L-alpha-alanineamide [e.g. Rahnella sp. H-7246 (FERM BP-2871)], DL-alpha-alanineamide or L-alpha-alanineamide is optically selectively hydrolyzed in an aqueous medium to obtain the objective compound, which is then taken from said aqueous medium.

3. 发明专利

JPH0690672A PRODUCTION OF COMPOSITION FOR RUMINANT 失效
公开(公告)号：JPH0690672A
公开(公告)日：1994-04-05
申请号：JP24315792
申请日：1992-09-11
申请人： KYOWA HAKKO KOGYO KK
发明人： YOKOMORI YOROZU , MURAYAMA SHUNICHI , SHODA TOMOAKI , OTA MOTOHIRO , AZUMA MASAYUKI , YUMIBA YOSHIO
IPC分类号： A61K9/28 , A61K9/50 , A23K1/16 , A23K1/18
摘要： PURPOSE:To provide a method for production of a composition for a ruminant, capable of carrying out in a working atmosphere where a large amount of an organic solvent is not used. CONSTITUTION:This method is carried out by coating a nucleus containing a bioactive substance with a coating material in which a hydrophobic substance sparingly soluble in water of >=pH5.5 and a polymer soluble in water of

4. 发明专利

JPH0638766A URICASE GENE AND PRODUCTION OF URICASE 失效
公开(公告)号：JPH0638766A
公开(公告)日：1994-02-15
申请号：JP32052591
申请日：1991-12-04
申请人： KYOWA HAKKO KOGYO KK
发明人： YAGASAKI MAKOTO , ISHINO SHUICHI , IWATA KAZUHISA , AZUMA MASAYUKI , TESHIBA SADAO , HASEGAWA MASARU , YAMAGUCHI KAZUO , YANO KEIICHI , YOKOO YOSHIHARU , HASHIMOTO YUKIO
IPC分类号： C12N1/20 , C12N1/21 , C12N9/06 , C12N15/09 , C12N15/53 , C12R1/01 , C12R1/19
摘要： PURPOSE:To abundantly provide uricase useful, e.g. for analysis of uric acid contained in blood or urine or as a kit for hair dyeing at a low cost. CONSTITUTION:Uricase is produced in a high yield by cloning a gene capable of coding uricase derived from a microorganism belonging to Cellulomonas, creating a recombinant plasmid containing the uricase gene, subsequently creating a transformat containing the plasmid and culturing the transformat.

5. 发明专利

JPH067183A PRODUCTION OF D-GLUTAMIC ACID 失效
公开(公告)号：JPH067183A
公开(公告)日：1994-01-18
申请号：JP15820692
申请日：1992-06-17
申请人： KYOWA HAKKO KOGYO KK
发明人： YAGASAKI MAKOTO , AZUMA MASAYUKI , OZAKI AKIO , HASHIMOTO YUKIO
IPC分类号： C12N1/20 , C12N1/21 , C12N9/90 , C12N15/09 , C12N15/61 , C12P13/14 , C12P41/00 , C12R1/19 , C12R1/24
摘要： PURPOSE:To enable the mass-production of D-glutamic acid useful as a synthetic raw material for pharmaceuticals, agricultural chemicals, etc., at a low cost. CONSTITUTION:D-glutamic acid can be produced by contacting L-glutamic acid with microbial cells, culture liquid or their treated product of a microbial strain belonging to the genus Escherichia and having glutamic acid racemase activity in an aqueous medium to produce DL-glutamic acid, contacting the DL-glutamic acid produced in the aqueous medium with microbial cells, culture liquid or their treated product of a microbial strain having L-glutamic acid decarboxylase activity to decompose the L-glutamic acid in the aqueous medium and collecting the remaining D-glutamic acid from the aqueous medium.

6. 发明专利

JPH10212185A PRODUCTION OF GRANULAR ORGANIC MATTER 失效
公开(公告)号：JPH10212185A
公开(公告)日：1998-08-11
申请号：JP1518197
申请日：1997-01-29
申请人： KYOWA HAKKO KOGYO KK
发明人： SODA AKIO , OKIURA TOMOO , AZUMA MASAYUKI
IPC分类号： C05F5/00 , C05F17/00 , C05G1/00
摘要： PROBLEM TO BE SOLVED: To obtain a physically stable granular organic matter reduced in the increase of adhesion generated in the production of the granular organic matter using a fermentation waste liquid by adding a settled fraction in an aerobically treated liquid obtained by aerating a methane fermented liquid in an aeration tank into the fermentation waste liquid. SOLUTION: The methane fermented liquid is obtained by methane-fermenting the fermentation waste water or an industrial waste at 30-60 deg.C for 3 days to 2 weeks. The methane fermented liquid is supplied to the aeration tank and aerated at the quantity of air flow of 0.1-1.2vol./vol./min for 1-7 days and after that, charged into a settler, the settled fraction is dehydrated, solar-dried to obtain a sludge having 15-55% solid portion. Next, 30-200wt/wt.% sludge (expressed in terms of the solid portion) and if necessary, one or more kind of inorganic fertilizers selected from phosphoric acid, diammonium phosphate, double super phosphate, super phosphate, potassium sulfate, potassium chloride and the like are added into the fermentation waste liquid concentrated to have 20-60% solid matter, granulated and dried.

7. 发明专利

JPH04183399A PRODUCTION OF D-PROLINE 失效
公开(公告)号：JPH04183399A
公开(公告)日：1992-06-30
申请号：JP31092190
申请日：1990-11-16
申请人： KYOWA HAKKO KOGYO KK
发明人： TAKADA HIDEYOSHI , HASHIMOTO YUKIO , AZUMA MASAYUKI , KAWAMOTO ISAO , FURUHATA IKUKO
IPC分类号： C12P41/00 , C12R1/645 , C12R1/72
摘要： PURPOSE:To efficiently produce D-proline at a low cost by reacting a culture of a microorganism, belonging to the genus Candida or Trichosporon and having hydrolytic activity of L-proline with DL-proline in an aqueous medium. CONSTITUTION:A culture of a microorganism, belonging to the genus Candida or Trichosporon and having the activity of hydrolyzing L-proline, microbial cells or their treated substances are reacted with DL-proline in an aqueous medium to selectively hydrolyze the L-proline and collect D-proline from the aforementioned aqueous medium. For example, Candida sp. PRD-238 (FERM BP-3159) or Trichosporon sp. PRD-317 (FERM BP-3160) is cited as the microorganism used. A culture medium used for culturing the microorganism may be a culture medium containing a carbon source, a nitrogen source, an inorganic substance, etc., assimilable by the microorganism used and capable of efficiently carrying out culturing of the microorganism having the ability to hydrolyze the L-proline.

8. 发明专利

JPS6030683A RESIN COMPOSITION CONTAINING IMMOBILIZED ENZYME, ITS PRODUCTION AND REGENERATION 失效
公开(公告)号：JPS6030683A
公开(公告)日：1985-02-16
申请号：JP13877483
申请日：1983-07-29
申请人： KYOWA HAKKO KOGYO KK
发明人： NAGASHIMA MINORU , AZUMA MASAYUKI , NOGUCHI SADAO
IPC分类号： C12N11/08
摘要： PURPOSE:To produce a regenerable resin composition containing immobilized enzyme, durable to the continuous use at a high substrate concentration and medium to high temperature, by using a basic anion exchange resin resistant to the deterioration of the resin and capable of reversibly adsorbing and desorbing the object to be treated. CONSTITUTION:An enzyme-containing liquid is made to contact with a basic anion exchange resin composed of styrene-divinylbenzene resin having an average macropore radius of 100-2,000Angstrom and a macropore volume of >=0.1ml/g, and having nitrogen-containing ion exchange group free from active hydrogen. The resin is made to contact with the substrate solution of said enzyme, and then with a polyvalent aldehyde such as glyoxal, etc. to obtain a resin composition containing enzymes mutually crosslinked with polyvalent aldehyde in the macropore of said resin. When the composition is used for a definite time interval, it is contacted with an aqueous solution of a mineral acid containing 0.2-5 M salt to effect the regeneration of said composition.

9. 发明专利

JPH07289275A PRODUCTION OF D-PROLINE 失效
公开(公告)号：JPH07289275A
公开(公告)日：1995-11-07
申请号：JP8701694
申请日：1994-04-26
申请人： KYOWA HAKKO KOGYO KK
发明人： YAGASAKI MAKOTO , OZAKI AKIO , AZUMA MASAYUKI , HASHIMOTO YUKIO , ISHINO SHUICHI
IPC分类号： C12N15/09 , C12N1/21 , C12P13/24 , C12R1/185 , C12R1/19
摘要： PURPOSE:To industrially produce D-proline used as a synthetic raw material for medicines, agrochemicals, etc. CONSTITUTION:This method for producing D-proline is to bring a microbial cell of a microorganism, belonging to the genus Escherichia and having proline racemase activities, a culture solution or a treated substance thereof into contact with L-proline in an aqueous medium, produce DL-proline, then bring the DL- proline produced in the aqueous medium into contact with a microbial cell of a microorganism having L-proline hydrolytic abilities, a culture solution or a treated substance thereof, hydrolyze the L-proline in the aqueous medium and then collect the residual D-proline from the aqueous medium.

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