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1. 发明申请

WO2005028654A1 METHOD FOR WHOLE SURROUNDING SURFACE DISPLAY OF TARGET PROTEINS USING BACTERIAL EXOSPORIUM 审中-公开
标题翻译：使用细菌外植体全面表面显示目标蛋白的方法
公开(公告)号：WO2005028654A1
公开(公告)日：2005-03-31
申请号：PCT/KR2003/002882
申请日：2003-12-30
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Tae Jung , PARK, Jong Pil , LEE, Seok Jae
发明人： LEE, Sang Yup , PARK, Tae Jung , PARK, Jong Pil , LEE, Seok Jae
IPC分类号： C12N15/63
CPC分类号： C40B40/02 , C12N15/1037
摘要： The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.
摘要翻译：本发明涉及在形成细菌孢子的最外表面的外孢上表达靶蛋白的方法。更具体地说，本发明涉及使用外鎓作为表面表达基质在细胞和孢子表面上表达靶蛋白的方法，以及用于产生蛋白质阵列，产生抗体的方法，分离特定于使用具有通过上述表达方法在表面表达的靶蛋白的细胞或孢子的来自混合物的某种物质，生物转化和靶蛋白的改良。根据本发明的用于在基因载体的孢子外膜的表面上表达靶蛋白的方法具有以下效果：可以表达多种靶蛋白，并且与目标蛋白的表面表达水平相比提高了靶蛋白的表达表达水平现有技术，以及表面上具有靶蛋白的基因载体的结构稳定性，宿主的活力以及筛选方法的快速性都大大提高。

2. 发明申请

WO2004061453A1 A PROTEIN CHIP FOR ANALYZING INTERACTION BETWEEN PROTEIN AND SUBSTRATE PEPTIDE THEREOF 审中-公开
标题翻译：蛋白质芯片，用于分析蛋白质与其底物之间的相互作用
公开(公告)号：WO2004061453A1
公开(公告)日：2004-07-22
申请号：PCT/KR2003/002183
申请日：2003-10-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Seok Jae
发明人： LEE, Sang Yup , LEE, Seok Jae
IPC分类号： G01N33/543
CPC分类号： C40B30/04 , C07K1/1077 , C07K14/5759 , C12N9/0006 , G01N33/54353 , G01N33/6803
摘要： The present invention relates to a protein chip of a S-L-SP form wherein a substrate peptide (SP) is immobilized on a solid substrate (S) by the mediation of a linker protein (L), as well as a method for analyzing the interaction between a reactive protein and its substrate peptide using such a protein chip. This analysis method for the interaction between a reactive protein and its substrate peptide using the protein chip comprises the steps of: adding a reactive protein to the protein chip, the reactive protein showing a specific interaction with the substrate protein immobilized on the protein chip; and detecting the interaction between the reactive protein and the substrate peptide. The present invention allows an increase in the reactivity between a peptide with low molecular weight and an enzyme with high molecular weight and between the peptide and a reactive antibody on the protein chip, so that the interaction between the peptide and the protein can be analyzed rapidly and massively.
摘要翻译：本发明涉及SL-SP形式的蛋白质芯片，其中通过连接蛋白（L）的介导将底物肽（SP）固定在固体底物（S）上，以及分析相互作用的方法在使用这种蛋白质芯片的反应蛋白和其底物肽之间。使用蛋白质芯片的反应蛋白与其底物肽之间的相互作用的分析方法包括以下步骤：向蛋白质芯片添加反应蛋白，反应蛋白与固定在蛋白质芯片上的底物蛋白显示特异性相互作用; 并检测反应蛋白和底物肽之间的相互作用。本发明允许增加低分子量肽和高分子量酶之间以及蛋白质芯片上的肽与反应性抗体之间的反应性，从而可以快速分析肽与蛋白质之间的相互作用并大量。

3. 发明申请

WO2007139283A1 METHOD FOR FABRICATING PHOTONIC-FLUIDIC BIOSENSOR USING FUNCTIONALIZED PHOTONIC CRYSTALS 审中-公开
标题翻译：使用功能化的光子晶体制造光子生物传感器的方法
公开(公告)号：WO2007139283A1
公开(公告)日：2007-12-06
申请号：PCT/KR2007/001874
申请日：2007-04-17
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , YANG, Seung-Man , LEE, Sang Yup , PARK, Tae Jung , LEE, Seung-Kon
发明人： YANG, Seung-Man , LEE, Sang Yup , PARK, Tae Jung , LEE, Seung-Kon
IPC分类号： G01N33/53
CPC分类号： G01N21/774
摘要： The present invention relates to a biosensor in which a substance capable of binding to biological materials is linked to a chemical functional group of functionalized photonic crystals to which the chemical functional group selected from the group of consisting of amine group (-NH2), aldehyde group (-CHO), and carboxyl group (-COOH) is bound, a photonic- fluidic fusion device including the biosensor, and a method for detecting target substances without a label using the biosensor or the photonic-fluidic fusion device.
摘要翻译：本发明涉及一种生物传感器，其中能够结合生物材料的物质与官能化光子晶体的化学官能团连接，化学官能团选自胺基（-NH 2），醛基（-CHO）和羧基（-COOH）结合，包括生物传感器的光子 - 流体融合装置，以及使用生物传感器或光子 - 流体融合装置检测没有标签的目标物质的方法。

4. 发明申请

WO2007136174A1 METHOD FOR PREPARING METAL NANOPARTICLE USING METAL BINDING PROTEIN 审中-公开
标题翻译：使用金属结合蛋白制备金属纳米颗粒的方法
公开(公告)号：WO2007136174A1
公开(公告)日：2007-11-29
申请号：PCT/KR2007/001865
申请日：2007-04-17
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Tae Jung
发明人： LEE, Sang Yup , PARK, Tae Jung
IPC分类号： C12N15/70 , C12N9/02
CPC分类号： C07K14/825 , B82Y5/00 , B82Y15/00 , C12N9/104 , C12P3/00 , G01N33/531 , G01N33/588 , Y10T428/2982
摘要： The present invention relates to a method of preparing heavy metal nanoparticles using a heavy metal-binding protein. More specifically, relates to a method for preparing heavy metal structures, comprising the steps of: culturing a microorganism transformed with a gene encoding a heavy metal-binding protein, in a heavy metal ion-containing medium, to produce heavy metal structures in the microorganism; and collecting the produced heavy metal structures, as well as nanoparticles of heavy metal structures prepared according to said method. Unlike prior methods of preparing quantum dots by physically binding metal materials, the quantum dots disclosed herein can be efficiently produced by expressing the heavy metal-binding protein in cells. In addition, the quantum dots are useful because they can solve an optical stability problem that is the shortcoming of organic fluorophores.
摘要翻译：本发明涉及使用重金属结合蛋白制备重金属纳米粒子的方法。更具体地，涉及一种制备重金属结构体的方法，包括以下步骤：在含重金属离子的培养基中培养用重金属结合蛋白的基因转化的微生物，以在微生物中产生重金属结构 ; 并收集所生产的重金属结构物，以及根据所述方法制备的重金属结构纳米颗粒。与通过物理结合金属材料制备量子点的现有方法不同，本文公开的量子点可以通过在细胞中表达重金属结合蛋白而有效地产生。此外，量子点是有用的，因为它们可以解决作为有机荧光团的缺点的光学稳定性问题。

5. 发明申请

WO2007078127A1 METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM 审中-公开
标题翻译：用BACILLUS ANTHRACIS EXOSPORIUM对目标蛋白进行细胞表面展示的方法
公开(公告)号：WO2007078127A1
公开(公告)日：2007-07-12
申请号：PCT/KR2006/005886
申请日：2006-12-29
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Tae Jung , HEO, Nam Su
发明人： LEE, Sang Yup , PARK, Tae Jung , HEO, Nam Su
IPC分类号： C12N15/64 , C12N15/70 , C12N15/63
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BcIA or fragements thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BcIA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BcIA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外孢菌蛋白质在微生物表面上表达靶蛋白质的方法。更具体地说，涉及这样构建的表达载体，即它包含编码炭疽芽孢杆菌外孢菌蛋白BcA1的bclA基因或其片段作为细胞表面锚定基序，并且靶蛋白可以在细胞表面表达当编码靶蛋白质的基因在宿主细胞中表达时与BcA1或其片段融合的形式，以及使用该载体在微生物表面表达靶蛋白质的方法。根据本发明的表达载体能够使用BcA1，炭疽芽孢杆菌外孢菌蛋白质作为细胞表面锚定基序在细胞表面上有效表达目标蛋白质或肽，并且由于靶蛋白质可以在细胞表面上稳定表达通过培养用表达载体转化的微生物大量培养，从而有可能有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

6. 发明申请

WO2009078687A3 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 审中-公开
公开(公告)号：WO2009078687A3
公开(公告)日：2009-06-25
申请号：PCT/KR2008/007533
申请日：2008-12-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
IPC分类号： C12N9/10 , C12N9/26 , C12N15/09 , C12N15/00
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

7. 发明申请

WO2008088104A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 审中-公开
标题翻译：具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法
公开(公告)号：WO2008088104A1
公开(公告)日：2008-07-24
申请号：PCT/KR2007/001263
申请日：2007-03-14
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
发明人： LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
IPC分类号： C12N1/16
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产力的突变微生物，以及使用突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

8. 发明申请

WO2006126750A1 METHOD FOR PREPARING A TARGET PROTEIN USING THE sHSPs 审中-公开
标题翻译：使用sHSP制备目标蛋白的方法
公开(公告)号：WO2006126750A1
公开(公告)日：2006-11-30
申请号：PCT/KR2005/001500
申请日：2005-05-23
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung
发明人： LEE, Sang Yup , HAN, Mee Jung
IPC分类号： C07K1/14
CPC分类号： C07K1/14
摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sHSPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.
摘要翻译：本发明涉及用于分离和纯化靶蛋白的方法，制备靶蛋白的方法，以及由全细胞酶或部分纯化的酶进行生物转化的方法。根据本发明，为了制备目标蛋白质的培养，分离纯化方法添加sHSP，可以通过防止蛋白酶的蛋白质损失而以高收率获得目标蛋白质。此外，当使用全细胞酶或部分纯化的酶在反应过程中加入sHSP时，可以通过防止蛋白酶损失酶来增加使用酶的生物转化产率。

9. 发明申请

WO2009008574A1 HOMO-SUCCINIC ACID PRODUCING MICROORGANISM VARIANT AND PROCESS FOR PREPARING SUCCINIC ACID USING THE SAME 审中-公开
标题翻译：制备微生物的肝细胞生长因子及其制备方法
公开(公告)号：WO2009008574A1
公开(公告)日：2009-01-15
申请号：PCT/KR2008/000241
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
发明人： LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
IPC分类号： C12N15/04 , C12N1/15
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydrogenase-encoding gene ( idhA ) and an acetate kinase-encoding gene ( ackA ), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏编码乳酸脱氢酶基因（idhA）和乙酸盐激酶编码基因（ackA）以及高浓度均聚琥珀酸的方法，包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

10. 发明申请

WO2008111708A1 L-THREONINE OVERPRODUCING MICROORGANISM AND METHOD FOR PREPARING L-THREONINE USING THE SAME 审中-公开
标题翻译： L-THREONINE OVERPRODUCTION MICROORGANISM AND METHOD FOR PRIPARING L-THREONINE WITH THE SAME
公开(公告)号：WO2008111708A1
公开(公告)日：2008-09-18
申请号：PCT/KR2007/003200
申请日：2007-07-02
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong
发明人： LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong
IPC分类号： C12N15/09 , C12N1/21 , C12P13/08
CPC分类号： C12P13/08
摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.
摘要翻译：本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

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