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1. 发明申请

US20090071832A1 MICROFLUIDIC DEVICE WITH VERTICAL INJECTION APERTURE 有权
标题翻译：具有垂直注射孔的微流体装置
公开(公告)号：US20090071832A1
公开(公告)日：2009-03-19
申请号：US11857570
申请日：2007-09-19
申请人： Jun Xie , Shashi Thutupalli , Stacey Joy Kennerly , Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Oliver Charles Boomhower
发明人： Jun Xie , Shashi Thutupalli , Stacey Joy Kennerly , Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Oliver Charles Boomhower
IPC分类号： G01N27/26
CPC分类号： G01N27/447
摘要： A microfluidic device with a vertical injection aperture is provided. The microfluidic device comprises a separation channel, an injection aperture disposed adjacent to and in fluid communication with the separation channel. The microfluidic device further comprises a semi-permeable filter disposed adjacent to the injection aperture, wherein the filter is configured to preconcentrate a sample in the injection aperture to form a preconcentrated sample plug during an injection operation, and wherein the sample plug flows downwardly from the injection aperture to the separation channel during an electrophoresis operation.
摘要翻译：提供具有垂直注射孔的微流体装置。微流体装置包括分离通道，与分离通道相邻并且与分离通道流体连通地设置的注射孔。微流体装置还包括邻近喷射孔布置的半渗透过滤器，其中过滤器构造成在注射操作期间预浓缩注入孔中的样品以形成预浓缩样品塞，并且其中样品塞从在电泳操作期间到分离通道的注射孔径。

2. 发明授权

US08029743B2 Microfluidic device with vertical injection aperture 有权
标题翻译：具有垂直注射孔径的微流体装置
公开(公告)号：US08029743B2
公开(公告)日：2011-10-04
申请号：US11857570
申请日：2007-09-19
申请人： Jun Xie , Shashi Thutupalli , Stacey Joy Kennerly , Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Oliver Charles Boomhower
发明人： Jun Xie , Shashi Thutupalli , Stacey Joy Kennerly , Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Oliver Charles Boomhower
IPC分类号： B01L3/00 , G01N27/453
CPC分类号： G01N27/447
摘要： A microfluidic device with a vertical injection aperture is provided. The microfluidic device comprises a separation channel, an injection aperture disposed adjacent to and in fluid communication with the separation channel. The microfluidic device further comprises a semi-permeable filter disposed adjacent to the injection aperture, wherein the filter is configured to preconcentrate a sample in the injection aperture to form a preconcentrated sample plug during an injection operation, and wherein the sample plug flows downwardly from the injection aperture to the separation channel during an electrophoresis operation.
摘要翻译：提供具有垂直注射孔的微流体装置。微流体装置包括分离通道，与分离通道相邻并且与分离通道流体连通地设置的注射孔。微流体装置还包括邻近喷射孔布置的半渗透过滤器，其中过滤器构造成在注射操作期间预浓缩注入孔中的样品以形成预浓缩样品塞，并且其中样品塞从在电泳操作期间到分离通道的注射孔径。

3. 发明申请

US20090166203A1 INJECTION METHOD FOR MICROFLUIDIC CHIPS 失效
标题翻译：微流感注射液的注射方法
公开(公告)号：US20090166203A1
公开(公告)日：2009-07-02
申请号：US11965794
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44743
摘要： A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.
摘要翻译：提供了用于毛细管电泳的微芯片。微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。所述微芯片还包括邻近所述“T”结和所述分离通道设置的第一阀，以及设置在所述“T”结处的第二阀。第二个阀是一个二通阀。将样品塞注入到“T”结和分离通道的第二端之间的区域中。

4. 发明申请

US20090166202A1 INJECTION METHOD FOR MICROFLUIDIC CHIPS 审中-公开
标题翻译：微流感注射液的注射方法
公开(公告)号：US20090166202A1
公开(公告)日：2009-07-02
申请号：US11965791
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： B01D57/02
CPC分类号： G01N27/44743 , G01N27/44791
摘要： A microchip for electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well. The injection channel and the separation channel form a ‘T’ junction. The microchip comprises a first electrode disposed at a first end of the separation channel, a second electrode disposed in front of the ‘T’ junction and adjacent to the first electrode, a third electrode disposed at a first end of the injection channel and a fourth electrode disposed at a second end of the separation channel. A portion of the sample is injected and separated into an area between the ‘T’ junction and the fourth electrode.
摘要翻译：提供用于电泳的微芯片。微芯片包括注入通道和配置成通过样品阱接收样品的分离通道。注入通道和分离通道形成“T”结。微芯片包括设置在分离通道的第一端的第一电极，设置在“T”结的前面并与第一电极相邻的第二电极，设置在注入通道的第一端的第三电极和第四电极电极设置在分离通道的第二端。样品的一部分被注入并分离成“T”结和第四电极之间的区域。

5. 发明授权

US08377277B2 System and method for performing microfluidic manipulation 失效
标题翻译：用于执行微流体操作的系统和方法
公开(公告)号：US08377277B2
公开(公告)日：2013-02-19
申请号：US12255962
申请日：2008-10-22
申请人： Jun Xie , Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout
发明人： Jun Xie , Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout
IPC分类号： B01D57/02
CPC分类号： G01N27/44791
摘要： Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir.
摘要翻译：电泳系统和方法包括电泳装置，其中电泳包括加载通道，分离通道和注射通道。装载通道与第一和第二采样端口流体连通。分离通道连接到装载通道以形成第一交叉点，以及连接到分离通道以形成第二交叉并与第一储存器流体连通的注入通道，并且其中分离通道与第二交换流体连通水库电泳系统还包括分别耦合到第一样品端口和第一储存器的两个电极以及适于将样品移动到装载通道中以朝向第一储存器形成样品塞的第一样品端口和第二储存器在分离通道中，并且进一步将样品塞进入分离通道朝向第二储存器。

6. 发明授权

US07740747B2 Injection method for microfluidic chips 失效
标题翻译：微流控芯片注射方法
公开(公告)号：US07740747B2
公开(公告)日：2010-06-22
申请号：US11965794
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： G01N27/26
CPC分类号： G01N27/44743
摘要： A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.
摘要翻译：提供了用于毛细管电泳的微芯片。微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。微芯片还包括邻近'T'结和在分离通道上设置的第一阀和设置在'T'结处的第二阀。第二个阀是一个二通阀。将样品塞注入到“T”结和分离通道的第二端之间的区域中。

7. 发明授权

US07740748B2 Electrophoresis system and method 失效
标题翻译：电泳系统及方法
公开(公告)号：US07740748B2
公开(公告)日：2010-06-22
申请号：US12258657
申请日：2008-10-27
申请人： Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout , Jun Xie
发明人： Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout , Jun Xie
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： B01D57/02 , B01L3/502715 , G01N27/44743
摘要： Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis device comprises a loading channel, an injection channel, and a separation channel. The loading channel is in fluid communication with a first and second sample port. The injection channel is connected to the loading channel to form a first intersection. The separation channel is connected to the injection channel to form a second intersection and in fluid communication with a first and second reservoir, and wherein the injection channel is in fluid communication with a third reservoir. The electrophoresis system further comprises electrodes coupled to the first sample port and the third reservoir, and the first reservoir and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the third reservoir and form a sample plug in the second intersection, and to further move the sample plug into the separation channel towards the second reservoir.
摘要翻译：电泳系统和方法包括电泳装置，其中电泳装置包括装载通道，注射通道和分离通道。装载通道与第一和第二采样端口流体连通。注入通道连接到装载通道以形成第一交叉。分离通道连接到注入通道以形成第二交叉并且与第一和第二储存器流体连通，并且其中注入通道与第三储存器流体连通。电泳系统还包括分别耦合到第一样品端口和第三储存器的电极，以及分别适于将样品移动到装载通道中的第一储存器和第二储存器，以朝向第三储存器形成样品塞并且将样品塞进一步朝向第二储存器移动到分离通道中。

8. 发明申请

US20100101951A1 ELECTROPHORESIS SYSTEM AND METHOD 失效
标题翻译：电泳系统和方法
公开(公告)号：US20100101951A1
公开(公告)日：2010-04-29
申请号：US12258657
申请日：2008-10-27
申请人： Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout , Jun Xie
发明人： Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout , Jun Xie
IPC分类号： B01D57/02
CPC分类号： B01D57/02 , B01L3/502715 , G01N27/44743
摘要： Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis device comprises a loading channel, an injection channel, and a separation channel. The loading channel is in fluid communication with a first and second sample port. The injection channel is connected to the loading channel to form a first intersection. The separation channel is connected to the injection channel to form a second intersection and in fluid communication with a first and second reservoir, and wherein the injection channel is in fluid communication with a third reservoir. The electrophoresis system further comprises electrodes coupled to the first sample port and the third reservoir, and the first reservoir and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the third reservoir and form a sample plug in the second intersection, and to further move the sample plug into the separation channel towards the second reservoir.
摘要翻译：电泳系统和方法包括电泳装置，其中电泳装置包括装载通道，注射通道和分离通道。装载通道与第一和第二采样端口流体连通。注入通道连接到装载通道以形成第一交叉。分离通道连接到注入通道以形成第二交叉并且与第一和第二储存器流体连通，并且其中注入通道与第三储存器流体连通。电泳系统还包括分别耦合到第一样品端口和第三储存器的电极，以及分别适于将样品移动到装载通道中的第一储存器和第二储存器，以朝向第三储存器形成样品塞并且将样品塞进一步朝向第二储存器移动到分离通道中。

9. 发明申请

US20100096267A1 SYSTEM AND METHOD FOR PERFORMING MICROFLUIDIC MANIPULATION 失效
标题翻译：用于执行微流控制的系统和方法
公开(公告)号：US20100096267A1
公开(公告)日：2010-04-22
申请号：US12255962
申请日：2008-10-22
申请人： Jun Xie , Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout
发明人： Jun Xie , Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout
IPC分类号： G01N27/26
CPC分类号： G01N27/44791
摘要： Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir.
摘要翻译：电泳系统和方法包括电泳装置，其中电泳包括加载通道，分离通道和注射通道。装载通道与第一和第二采样端口流体连通。分离通道连接到装载通道以形成第一交叉点，以及连接到分离通道以形成第二交叉并与第一储存器流体连通的注入通道，并且其中分离通道与第二交换流体连通水库电泳系统还包括分别耦合到第一样品端口和第一储存器的两个电极以及适于将样品移动到装载通道中以朝向第一储存器形成样品塞的第一样品端口和第二储存器在分离通道中，并且进一步将样品塞进入分离通道朝向第二储存器。

10. 发明申请

US20090166201A1 INJECTION METHOD FOR MICROFLUIDIC CHIPS 审中-公开
标题翻译：微流感注射液的注射方法
公开(公告)号：US20090166201A1
公开(公告)日：2009-07-02
申请号：US11965789
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44743 , B01L3/502715
摘要： A microfluidic chip comprising a separation channel configured to receive a sieving matrix and a buffer and an injection channel in fluid communication with the separation channel. The injection channel is configured to receive a sample using a capillary force and a portion of the sample injects into the separation channel electro-kinetic force exerted on the sample.
摘要翻译：一种微流体芯片，包括被配置为接收筛分基质和缓冲液的分离通道和与分离通道流体连通的注入通道。注射通道被配置为使用毛细管力接收样品，并且样品的一部分注入施加在样品上的分离通道电动力。

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