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1. 发明申请

US20120135449A1 ITERATIVE STAINING OF BIOLOGICAL SAMPLES 审中-公开
标题翻译：生物样本的迭代染色
公开(公告)号：US20120135449A1
公开(公告)日：2012-05-31
申请号：US13365525
申请日：2012-02-03
申请人： Jun Xie , Robert John Filkins , Fiona Mary Ginty , Michael Christopher Montalto , Anup Sood , Jeffrey Bernard Fortin , Wei-Cheng Tian , Michael John Gerdes
发明人： Jun Xie , Robert John Filkins , Fiona Mary Ginty , Michael Christopher Montalto , Anup Sood , Jeffrey Bernard Fortin , Wei-Cheng Tian , Michael John Gerdes
IPC分类号： G01N21/75 , C12M1/34
CPC分类号： G01N1/312
摘要： Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent, and releasing the sample from the flow cell wherein the integrity of the sample is intact. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.
摘要翻译：提供了促进成像应用中生物样品的迭代染色的自动化方法和装置。所述方法包括以下步骤：提供含有生物样品的小体积流动池，向生物样品施加染色，将至少两种前体试剂结合以形成活化的脱色剂，并且其中所述活化脱色剂分解速率大于或等于以脱色反应速率使脱色剂以大于活化脱色剂的分解速率的流速流过生物样品，并将样品从流动池中释放出来，其中样品的完整性是完整的。染色，组合和流动的过程可以迭代地重复。本文还公开了用于重复染色生物样品的装置，其包括与预混合器流体连通的流动池，其中预混合器的体积容量小于流动池的体积容量的约五倍。

2. 发明申请

US20090253163A1 ITERATIVE STAINING OF BIOLOGICAL SAMPLES 审中-公开
标题翻译：生物样本的迭代染色
公开(公告)号：US20090253163A1
公开(公告)日：2009-10-08
申请号：US12061044
申请日：2008-04-02
申请人： Jun Xie , Fiona Ginty , Robert John Filkins , Michael Christopher Montalto , Anup Sood , Jeffrey Bernard Fortin , Wei-Cheng Tian , Michael John Gerdes
发明人： Jun Xie , Fiona Ginty , Robert John Filkins , Michael Christopher Montalto , Anup Sood , Jeffrey Bernard Fortin , Wei-Cheng Tian , Michael John Gerdes
IPC分类号： G01N1/30 , C12M1/34 , C12M1/36
CPC分类号： G01N1/312
摘要： Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, and flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.
摘要翻译：提供了促进成像应用中生物样品的迭代染色的自动化方法和装置。所述方法包括以下步骤：提供含有生物样品的小体积流动池，向生物样品施加染色，将至少两种前体试剂结合以形成活化的脱色剂，并且其中所述活化脱色剂分解速率大于或等于与脱色反应速率相关联，并使脱色剂以大于活化脱色剂分解速率的流速流过生物样品。染色，组合和流动的过程可以迭代地重复。本文还公开了用于重复染色生物样品的装置，其包括与预混合器流体连通的流动池，其中预混合器的体积容量小于流动池的体积容量的约五倍。

3. 发明申请

US20100059120A1 MICROFLUIDIC DEVICE AND METHODS FOR DROPLET GENERATION AND MANIPULATION 审中-公开
标题翻译：微流体装置及其产生和操作的方法
公开(公告)号：US20100059120A1
公开(公告)日：2010-03-11
申请号：US12208445
申请日：2008-09-11
申请人： Wei-Cheng Tian , Jeffrey Bernard Fortin , Jun Xie , Barbara Grossman , Oliver Charles Boomhower , Shashi Thutupalli , Long Que
发明人： Wei-Cheng Tian , Jeffrey Bernard Fortin , Jun Xie , Barbara Grossman , Oliver Charles Boomhower , Shashi Thutupalli , Long Que
IPC分类号： G01N33/00
CPC分类号： B01L3/502738 , B01L3/502707 , B01L3/502784 , B01L2200/0605 , B01L2200/0673 , B01L2300/0874 , B01L2300/161 , B01L2400/0415 , B01L2400/043 , B01L2400/0475 , B01L2400/0655 , B01L2400/0672 , Y10T137/0318
摘要： Methods and microfluidic devices for generating and manipulating sample droplets, wherein the devices comprise, a plurality of fluid channels, at least one of which is a sample channel for carrying a fluidic sample material, that is in fluid communication with the carrier fluid channel via an orifice; and an actuated flow interrupter adapted to force a predetermined amount of the sample fluid from the sample channel through the orifice into the carrier fluid channel.
摘要翻译：用于产生和操纵样品液滴的方法和微流体装置，其中所述装置包括多个流体通道，其中至少一个流体通道是用于承载流体样品材料的样品通道，其经由载体流体通道流体连通孔; 以及致动流动断路器，其适于将预定量的样品流体从样品通道穿过所述孔进入载体流体通道。

4. 发明授权

US07101789B2 Method of wet etching vias and articles formed thereby 失效
公开(公告)号：US07101789B2
公开(公告)日：2006-09-05
申请号：US10938247
申请日：2004-09-13
申请人： Kanakasabapathi Subramanian , Jeffrey Bernard Fortin , Wei-Cheng Tian
发明人： Kanakasabapathi Subramanian , Jeffrey Bernard Fortin , Wei-Cheng Tian
IPC分类号： H01L21/44
CPC分类号： H01L21/30617 , H01L21/76898 , H01L23/481 , H01L2223/54453 , H01L2924/0002 , H01L2924/00
摘要： A method for forming smooth walled, prismatically-profiled through-wafer vias and articles formed through the method. An etch stop material is provided on a wafer, which may be a silicon wafer. A mask material is provided on the etch stop material and patterned in such a way as to lead to the formation of vias that have at least one pair of opposing side walls that run parallel to a plane in the wafer. A wet etchant, such as potassium hydroxide, is used to etch vias in the wafer. The use of a wet etchant leads to the formation of smooth side walls. This method allows an aspect ratio of height versus width of the vias of greater than 75 to 1.

5. 发明授权

US07545012B2 Capacitive micromachined ultrasound transducer fabricated with epitaxial silicon membrane 有权
标题翻译：用外延硅膜制造的电容式微机械超声换能器
公开(公告)号：US07545012B2
公开(公告)日：2009-06-09
申请号：US11393317
申请日：2006-03-30
申请人： Lowell Scott Smith , David Martin Mills , Jeffrey Bernard Fortin , Wei-Cheng Tian , John Robert Logan
发明人： Lowell Scott Smith , David Martin Mills , Jeffrey Bernard Fortin , Wei-Cheng Tian , John Robert Logan
IPC分类号： H01L29/82 , H01L41/02 , H04R17/00
CPC分类号： B06B1/0292
摘要： A capacitive micromachined ultrasound transducer (cMUT) cell is presented. The cMUT cell includes a lower electrode. Furthermore, the cMUT cell includes a diaphragm disposed adjacent to the lower electrode such that a gap having a first gap width is formed between the diaphragm and the lower electrode, wherein the diaphragm comprises one of a first epitaxial layer or a first polysilicon layer. In addition, a stress reducing material is disposed in the first epitaxial layer.
摘要翻译：提出了一种电容式微机械超声换能器（cMUT）。 cMUT电池包括下电极。此外，cMUT单元包括邻近下电极设置的隔膜，使得在隔膜和下电极之间形成具有第一间隙宽度的间隙，其中隔膜包括第一外延层或第一多晶硅层中的一个。此外，应力降低材料设置在第一外延层中。

6. 发明授权

US07365437B2 Method of wet etching vias and articles formed thereby 失效
标题翻译：湿法蚀刻通孔和由此形成的制品的方法
公开(公告)号：US07365437B2
公开(公告)日：2008-04-29
申请号：US11337249
申请日：2006-01-23
申请人： Kanakasabapathi Subramanian , Jeffrey Bernard Fortin , Wei-Cheng Tian
发明人： Kanakasabapathi Subramanian , Jeffrey Bernard Fortin , Wei-Cheng Tian
IPC分类号： H01L29/40
CPC分类号： H01L21/30617 , H01L21/76898 , H01L23/481 , H01L2223/54453 , H01L2924/0002 , H01L2924/00
摘要： A method for forming smooth walled, prismatically-profiled through-wafer vias and articles formed through the method. An etch stop material is provided on a wafer, which may be a silicon wafer. A mask material is provided on the etch stop material and patterned in such a way as to lead to the formation of vias that have at least one pair of opposing side walls that run parallel to a plane in the wafer. A wet etchant, such as potassium hydroxide, is used to etch vias in the wafer. The use of a wet etchant leads to the formation of smooth side walls. This method allows an aspect ratio of height versus width of the vias of greater than 75 to 1.
摘要翻译：用于形成通过该方法形成的平滑壁，棱镜通孔晶片通孔和制品的方法。在晶片上提供蚀刻停止材料，晶片可以是<110>硅晶片。在蚀刻停止材料上提供掩模材料，并以这样的方式图案化，以便导致形成通孔，其具有平行于晶片中的111平面延伸的至少一对相对的侧壁。使用诸如氢氧化钾的湿蚀刻剂来蚀刻晶片中的通孔。使用湿蚀刻剂导致形成平滑的侧壁。该方法允许通孔的高度与宽度的长宽比大于75至1。

7. 发明授权

US07037746B1 Capacitive micromachined ultrasound transducer fabricated with epitaxial silicon membrane 失效
公开(公告)号：US07037746B1
公开(公告)日：2006-05-02
申请号：US11023252
申请日：2004-12-27
申请人： Lowell Scott Smith , David Martin Mills , Jeffrey Bernard Fortin , Wei-Cheng Tian , John Robert Logan
发明人： Lowell Scott Smith , David Martin Mills , Jeffrey Bernard Fortin , Wei-Cheng Tian , John Robert Logan
IPC分类号： H01L21/00
CPC分类号： B06B1/0292
摘要： A capacitive micromachined ultrasound transducer (cMUT) cell is presented. The cMUT cell includes a lower electrode. Furthermore, the cMUT cell includes a diaphragm disposed adjacent to the lower electrode such that a gap having a first gap width is formed between the diaphragm and the lower electrode, wherein the diaphragm comprises one of a first epitaxial layer or a first polysilicon layer. In addition, a stress reducing material is disposed in the first epitaxial layer.

8. 发明授权

US07927476B2 Injection method for microfluidic chips 有权
标题翻译：微流控芯片注射方法
公开(公告)号：US07927476B2
公开(公告)日：2011-04-19
申请号：US12341233
申请日：2008-12-22
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： B01D57/02
摘要： Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.
摘要翻译：用于在电泳期间注射样品的方法和系统，其通常包括：通过分离通道的第一端装载筛分基质; 筛分矩阵的结束距离分离通道和加载通道的交点处一定距离; 通过加载通道加载样品并填充分离通道的空的部分; 在使洗涤缓冲液流过所述装载通道的同时，在所述分离通道上施加电场; 以及将所述样品的一部分注入所述分离通道中，其中所述注入的所述样品的所述部分具有由所述筛分矩阵的末端与所述加载和分离通道的交点之间的距离确定的尺寸。

9. 发明申请

US20090166203A1 INJECTION METHOD FOR MICROFLUIDIC CHIPS 失效
标题翻译：微流感注射液的注射方法
公开(公告)号：US20090166203A1
公开(公告)日：2009-07-02
申请号：US11965794
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44743
摘要： A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.
摘要翻译：提供了用于毛细管电泳的微芯片。微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。所述微芯片还包括邻近所述“T”结和所述分离通道设置的第一阀，以及设置在所述“T”结处的第二阀。第二个阀是一个二通阀。将样品塞注入到“T”结和分离通道的第二端之间的区域中。

10. 发明申请

US20090166202A1 INJECTION METHOD FOR MICROFLUIDIC CHIPS 审中-公开
标题翻译：微流感注射液的注射方法
公开(公告)号：US20090166202A1
公开(公告)日：2009-07-02
申请号：US11965791
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： B01D57/02
CPC分类号： G01N27/44743 , G01N27/44791
摘要： A microchip for electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well. The injection channel and the separation channel form a ‘T’ junction. The microchip comprises a first electrode disposed at a first end of the separation channel, a second electrode disposed in front of the ‘T’ junction and adjacent to the first electrode, a third electrode disposed at a first end of the injection channel and a fourth electrode disposed at a second end of the separation channel. A portion of the sample is injected and separated into an area between the ‘T’ junction and the fourth electrode.
摘要翻译：提供用于电泳的微芯片。微芯片包括注入通道和配置成通过样品阱接收样品的分离通道。注入通道和分离通道形成“T”结。微芯片包括设置在分离通道的第一端的第一电极，设置在“T”结的前面并与第一电极相邻的第二电极，设置在注入通道的第一端的第三电极和第四电极电极设置在分离通道的第二端。样品的一部分被注入并分离成“T”结和第四电极之间的区域。

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