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    • 5. 发明授权
    • Process of making cyclodextrin glucanotransferase with brevibacterium
    • 用短杆菌制备环糊精葡聚糖转移酶的方法
    • US5707833A
    • 1998-01-13
    • US471235
    • 1995-06-06
    • Shigeharu MoriTamio MaseTakaichi Ohya
    • Shigeharu MoriTamio MaseTakaichi Ohya
    • C12N9/10C12P19/18C12P21/04
    • C12N9/1074C12P19/18Y10S435/84
    • This invention relates to a .gamma.-cyclodextrin glucanotransferase having novel properties, to a process for the production of the .gamma.-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the .gamma.-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of .gamma.-cyclodextrin as a main product, and to a method for increasing .gamma.-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which .gamma.-cyclodextrin and .beta.-cyclodextrin are formed from starch by the action of .gamma.-cyclodextrin glucanotransferase, thereby effecting repressed formation of .beta.-cyclodextrin and enhanced formation of .gamma.-cyclodextrin. Development of the process of this invention has rendered possible low cost production of .gamma.-cyclodextrin and therefore expands practical use of .gamma.-cyclodextrin to the field of food and the like.
    • 本发明涉及一种具有新特性的γ-环糊精葡聚糖转移酶,涉及γ-环糊精葡聚糖转移酶的制备方法,该方法包括培养能产生环糊精葡聚糖转移酶的短杆菌属属的菌株,从而使菌株产生γ- 环糊精葡聚糖转移酶,然后将酶收集到生产环糊精的方法中,该方法包括使环糊精葡聚糖转移酶与溶解在溶液中的底物反应,从而形成γ-环糊精作为主要产物,并且 一种增加γ-环糊精产率而不伴随产生总环糊精的增加的方法,该方法包括向γ-淀粉酶通过γ-环糊精葡聚糖转移酶的作用从淀粉形成γ-环糊精和β-环糊精的反应溶液中加入乙醇, 形成β-环糊精并增强γ-环糊精的形成。 本发明方法的开发使得γ-环糊精的低成本生产成为可能,从而将γ-环糊精的实际应用扩展到食品领域等。