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    • 2. 发明公开
    • METHOD FOR TREATMENT OF TOOLS AND TOOLS USED FOR ISOLATION OF MICROVESICLES, NANOVESICLES OR EXSOMES
    • 处理用于隔离微泡,纳米材料或外来物的工具和工具的方法
    • EP3185921A1
    • 2017-07-05
    • EP15767421.9
    • 2015-08-25
    • Jozef Stefan InstituteUniverza v Ljubljani
    • JUNKAR, ItaMOZETIC, MiranZAPLOTNIK, RokKRALJ-IGLIC, VeronikaTUKELJ, Roman
    • A61L29/14A61L33/00A61M1/02A61M5/14B05D3/14
    • A61L29/14A61L33/0094A61L2400/18
    • According to the present invention a method which prevents excessive adsorption of microvesicles on the surfaces of tools used for sampling, storing and handling body flu- ids containing microvesicles is provided. The method comprises the steps of: selecting said a tool from the a list plurality of tools including but not limited to needles, blood tubing, blood bags, catheters, Eppendorf tubes, pipettes or the like, providing said tool from said plurality of tools, providing a source of positively and negatively charged particles of high density, selecting a source assuring for formation of positively and negatively charged particles of high density and treating a surface of said tool by applying short pulses of said source of particles next to or on the said surface of said tool to assure surface modification of said surface by reacting said positively and negatively charged particles of high density on said surface. The method according to the present invention ensures contacting of tools with short pulses of highly ionized gas comprising both positively and negatively charged particles, the pulses being essentially short enough to avoid excessive heating of materials used for collecting, sampling, storage, transport and isolation of micro vesicles and the density of both positively and negatively charged particles which is essentially high enough to cause roughening of said tools on sub-micrometer or nanometer scale. Especially tools treated according to the present inventive method prevents excessive adsorption of microvesicles on the surfaces of said tools used for collecting, sampling, storing, transporting and isolating of microvesicles or the like. The method according to the present invention enables higher yields and lower frag- mentation of microvesicles for instance by preventing adsorption of this valuable diag- nostic material on the surface of different tools used for isolation and detection. Accordingly, the present invention also provides increasing the roughness of a material by the method according to the present invention. In particular the material which is used to produce diagnostic or medical tools or devices. (Fig. 1)
    • 根据本发明,提供了防止微囊过度吸附在用于取样,储存和处理含有微泡的体液的工具表面上的方法。 该方法包括以下步骤:从列表中选择所述工具,所述工具包括但不限于针,血液管,血袋,导管,Eppendorf管,移液管等,提供来自所述多个工具的所述工具, 提供具有高密度的带正电和带负电粒子的来源,选择确保形成高密度带正电荷和负带电粒子的源,并通过在所述场的旁边或上面施加所述粒子源的短脉冲来处理所述工具的表面 所述工具的表面以通过使所述表面上的所述高密度正电荷和负电荷颗粒反应来确保所述表面的表面改性。 根据本发明的方法确保工具与包含带正电和带负电的颗粒的高度电离气体的短脉冲的接触,脉冲本质上足够短以避免用于收集,取样,储存,运输和隔离的材料的过度加热 微囊泡以及带正电和带负电粒子的密度,其基本上足够高以致在亚微米或纳米尺度上使所述工具粗糙化。 特别是根据本发明方法处理的工具防止微囊过度吸附在用于收集,取样,储存,运输和分离微泡等的所述工具的表面上。 根据本发明的方法例如通过防止这种有价值的诊断材料在用于分离和检测的不同工具的表面上的吸附而实现更高的收率和更低的微泡破碎。 因此,本发明还通过根据本发明的方法提供增加材料的粗糙度。 特别是用于生产诊断或医疗工具或设备的材料。 (图。1)