专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08999674B2 Methods and apparatus for single molecule sequencing using energy transfer detection 有权
标题翻译：使用能量转移检测的单分子测序的方法和装置
公开(公告)号：US08999674B2
公开(公告)日：2015-04-07
申请号：US13562159
申请日：2012-07-30
申请人： Joseph Beechem , Theo Nikiforov , Vi-En Choong , Xinzhan Peng , Guobin Luo , Cheng-Yao Chen , Michael Previte
发明人： Joseph Beechem , Theo Nikiforov , Vi-En Choong , Xinzhan Peng , Guobin Luo , Cheng-Yao Chen , Michael Previte
IPC分类号： C12P19/34 , C12N9/12 , C07H19/20
CPC分类号： C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
摘要： Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.
摘要翻译：本文提供了用于核苷酸掺入反应的系统和方法。该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。

2. 发明申请

US20100255487A1 METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION 审中-公开
标题翻译：使用能量转移检测的单分子序列的方法和装置
公开(公告)号：US20100255487A1
公开(公告)日：2010-10-07
申请号：US12748168
申请日：2010-03-26
申请人： JOSEPH BEECHEM , THEO NIKIFOROV , Vi-En Choong , Xinzhan Peng , Guobin Luo , Cheng-Yao Chen , Michael Previte
发明人： JOSEPH BEECHEM , THEO NIKIFOROV , Vi-En Choong , Xinzhan Peng , Guobin Luo , Cheng-Yao Chen , Michael Previte
IPC分类号： C12Q1/68 , C12N9/10
CPC分类号： C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
摘要： Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.
摘要翻译：本文提供了用于核苷酸掺入反应的系统和方法。该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。

3. 发明授权

US08632975B2 Nucleotide transient binding for sequencing methods 有权
标题翻译：测序方法的核苷酸瞬变结合
公开(公告)号：US08632975B2
公开(公告)日：2014-01-21
申请号：US12790760
申请日：2010-05-28
申请人： Peter B. Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
发明人： Peter B. Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
摘要翻译：本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

4. 发明申请

US20100330570A1 NUCLEOTIDE TRANSIENT BINDING FOR SEQUENCING METHODS 有权
标题翻译：用于测序方法的核心瞬态绑定
公开(公告)号：US20100330570A1
公开(公告)日：2010-12-30
申请号：US12790760
申请日：2010-05-28
申请人： Peter B. VANDER HORN , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
发明人： Peter B. VANDER HORN , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
IPC分类号： C12Q1/68 , C12N9/96
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
摘要翻译：本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

5. 发明授权

US09353412B2 Conformational probes and methods for sequencing nucleic acids 有权
标题翻译：构象探针和核酸测序方法
公开(公告)号：US09353412B2
公开(公告)日：2016-05-31
申请号：US13162325
申请日：2011-06-16
申请人： Molly He , Cheng-Yao Chen , Eric Kool , Mostafa Ronaghi , Michael Previte , Rigo Pantoja
发明人： Molly He , Cheng-Yao Chen , Eric Kool , Mostafa Ronaghi , Michael Previte , Rigo Pantoja
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6818 , C12Q2565/133 , C12Q2563/125 , C12Q2525/101 , C12Q2565/1015
摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.
摘要翻译：本公开提供了确定核酸模板的核苷酸序列的方法。该方法可以包括以下步骤：使构象标记的聚合酶和至少四种不同的核苷酸物种在其中所述构象标记的聚合酶催化核苷酸物种的顺序加入以形成核酸模板的核酸补体的条件下接触，其中每个不同核苷酸类型的顺序加入从所述构象标记的聚合酶产生构象信号变化，并且其中所述构象信号变化的速率或持续时间对于每个不同的核苷酸种类是可区分的; 在条件下检测来自构象标记的聚合酶的信号的一系列变化; 并确定信号变化的速率或时间，从而确定核酸模板的核苷酸序列。

6. 发明授权

US08008067B2 Microwave trigger metal-enhanced chemiluminescence (MT MEC) and spatial and temporal control of same 有权
标题翻译：微波触发金属增强化学发光（MT MEC）和空间与时间控制相同
公开(公告)号：US08008067B2
公开(公告)日：2011-08-30
申请号：US12036402
申请日：2008-02-25
申请人： Chris D. Geddes , Michael Previte
发明人： Chris D. Geddes , Michael Previte
IPC分类号： C12M1/34
CPC分类号： G01N21/763 , G01N21/76 , G01N33/54306 , G01N33/5438 , G01N33/553 , Y10T436/143333 , Y10T436/144444 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666 , Y10T436/21 , Y10T436/23
摘要： The present invention relates to a method of imaging structures and features using plasmonic emissions from metallic surfaces caused by chemiluminescence based chemical and biological reactions wherein imaging of the reactions is enhanced by the use of microwave energy and further enhanced by using metallic geometric structures for spatially and temporally controlling the biological and chemical reactions.
摘要翻译：本发明涉及使用由化学发光化学和生物反应引起的金属表面等离子体发射成像结构和特征的方法，其中通过使用微波能量增强反应的成像，并通过使用金属几何结构进一步增强空间和在时间上控制生物和化学反应。

7. 发明授权

US09075018B2 Microwave trigger metal-enhanced chemiluminescence (MT MEC) and spatial and temporal control of same 有权
标题翻译：微波触发金属增强化学发光（MT MEC）和空间与时间控制相同
公开(公告)号：US09075018B2
公开(公告)日：2015-07-07
申请号：US13218890
申请日：2011-08-26
申请人： Chris D. Geddes , Michael Previte
发明人： Chris D. Geddes , Michael Previte
IPC分类号： G01N21/01 , G01N21/76 , G01N33/543 , G01N33/553
CPC分类号： G01N21/763 , G01N21/76 , G01N33/54306 , G01N33/5438 , G01N33/553 , Y10T436/143333 , Y10T436/144444 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666 , Y10T436/21 , Y10T436/23
摘要： The present invention relates to a method of imaging structures and features using plasmonic emissions from metallic surfaces caused by chemiluminescence based chemical and biological reactions wherein imaging of the reactions is enhanced by the use of microwave energy and further enhanced by using metallic geometric structures for spatially and temporally controlling the biological and chemical reactions.
摘要翻译：本发明涉及使用由化学发光化学和生物反应引起的金属表面等离子体发射成像结构和特征的方法，其中通过使用微波能量增强反应的成像，并通过使用金属几何结构进一步增强空间和在时间上控制生物和化学反应。

8. 发明申请

US20060057497A1 Apparatus for three-dimensional optical data storage and retrieval 失效
标题翻译：三维光学数据存储和检索装置
公开(公告)号：US20060057497A1
公开(公告)日：2006-03-16
申请号：US11269016
申请日：2005-11-08
申请人： John Fourkas , Christopher Olson , Michael Previte
发明人： John Fourkas , Christopher Olson , Michael Previte
IPC分类号： G11B7/24 , C07D305/00
CPC分类号： C07D307/885 , Y10S430/146
摘要： A three dimensional optical data storage and retrieval system that includes a three dimensional optical data storage medium and an apparatus for providing access to data stored on the medium. The data storage medium includes an optical data storage material which either a low molecular weight or polymeric glassy solid that are capable of undergoing multi-photon excitation that are energetically different in the write and read cycles. The optical data storage materials provide substantially higher storage capacities relative to conventional materials, and show high robustness in that written and stored data can undergo multiple read cycles without erasure or overwriting. An apparatus for data recording and accessing stored data on the medium includes a controllable variable energy photo-emitting excitation source and an emission photo-detector.
摘要翻译：一种三维光学数据存储和检索系统，其包括三维光学数据存储介质和用于提供对存储在介质上的数据的访问的设备。数据存储介质包括光学数据存储材料，其可以在能够在写入和读取周期中在能量上不同的能够经历多光子激发的低分子量或聚合物玻璃状固体。光学数据存储材料相对于常规材料提供了更高的存储容量，并且显示出高的鲁棒性，因为所写入和存储的数据可经历多个读取周期而不擦除或重写。用于数据记录和访问介质上存储数据的装置包括可控可变能量发光激发源和发射光检测器。

9. 发明申请

US20100003695A1 MICROWAVE TRIGGER METAL-ENHANCED CHEMILUMINESCENCE (MT MEC) AND SPATIAL AND TEMPORAL CONTROL OF SAME 有权
标题翻译：微波触发器金属增强发光（MT MEC）及其空间和时间控制
公开(公告)号：US20100003695A1
公开(公告)日：2010-01-07
申请号：US12036402
申请日：2008-02-25
申请人： Chris D. Geddes , Michael Previte
发明人： Chris D. Geddes , Michael Previte
IPC分类号： G01N33/53 , G01N21/76 , G01N33/00 , G01N33/50 , G01N33/48 , G01N33/03 , C12M1/34
CPC分类号： G01N21/763 , G01N21/76 , G01N33/54306 , G01N33/5438 , G01N33/553 , Y10T436/143333 , Y10T436/144444 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666 , Y10T436/21 , Y10T436/23
摘要： The present invention relates to a method of imaging structures and features using plasmonic emissions from metallic surfaces caused by chemiluminescence based chemical and biological reactions wherein imaging of the reactions is enhanced by the use of microwave energy and further enhanced by using metallic geometric structures for spatially and temporally controlling the biological and chemical reactions.
摘要翻译：本发明涉及使用由化学发光化学和生物反应引起的金属表面等离子体发射成像结构和特征的方法，其中通过使用微波能量增强反应的成像，并通过使用金属几何结构进一步增强空间和在时间上控制生物和化学反应。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式