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1. 发明授权

US10428994B2 Auto-fill two-layer two-half wear resistant elbow of concrete pump truck and manufacturing method thereof 有权
公开(公告)号：US10428994B2
公开(公告)日：2019-10-01
申请号：US15523564
申请日：2016-01-28
申请人： Zhaohui Zhou
发明人： Zhaohui Zhou
IPC分类号： F16L57/06 , B65G53/52 , F16L43/00 , E04G21/04 , F16L9/19
摘要： An auto-fill two-layer two-half wear resistant elbow of a concrete pump truck consists of an outer-layer protection elbow, an inner-layer heterogeneous wear resistant combined elbow and a wear resistant connecting flange. A filling space is provided between the outer-layer protection elbow and the inner-layer heterogeneous wear resistant combined elbow. The wear resistant elbow is designed as a two-layer two-half unique structure including a protection layer and a wear resistant layer. Upon using the wear resistant elbow for the first time, the concrete grout fills the reserved slit via the heterogenous combined elbow and enters the reserved filling space, allowing the outer-layer protection elbow and the inner-layer heterogeneous wear resistant combined elbow to be fixed as a whole.

2. 发明申请

US20100291605A1 ASSAYS FOR S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES 审中-公开
标题翻译： S-腺嘌呤依赖性甲基衍生物的测定
公开(公告)号：US20100291605A1
公开(公告)日：2010-11-18
申请号：US12161795
申请日：2007-01-23
申请人： Zhaohui Zhou , Kathleen Dorgan
发明人： Zhaohui Zhou , Kathleen Dorgan
IPC分类号： C12Q1/48 , C12Q1/34
CPC分类号： C12Q1/48 , C12Q1/52 , G01N2333/91011
摘要： Disclosed are novel methyltransferase assay methods, comprising: including, in a reaction mixture for a methyltransferase activity, a purified or recombinant adenosine nucleosidase activity that catalyses release of an adenine or adenine derivative moiety from a transmethylation product, and a purified or recombinant adenine deaminase activity that catalyses deamination of the released moiety to hypoxanthine or respective derivative and ammonia, wherein the methyltransferase activity is rate-limiting; and determining the methyltransferase activity by spectrophotometric or chromatographic monitoring of the coupled deamination reaction products, or of subsequent enzymatic or chemical reactions coupled thereto. Coupled oxidation of the hypoxanthine to uric acid and hydrogen peroxide is optionally affected using purified or recombinant xanthine oxidase, wherein the methyltransferase activity is rate-limiting, and wherein determining the methyltransferase activity comprises monitoring of the coupled oxidation reaction. Variations are disclosed comprises monitoring of reaction products (e.g., to detect NH3, Hypoxanthine, H2O2, and Uric Acid).
摘要翻译：公开了新的甲基转移酶测定方法，其包括：在用于甲基转移酶活性的反应混合物中，包含催化腺嘌呤或腺嘌呤衍生物部分从转甲基化产物释放的纯化或重组腺苷核苷酶活性，以及纯化或重组腺嘌呤脱氨酶活性催化释放的部分与次黄嘌呤或相应的衍生物和氨的脱氨基，其中甲基转移酶活性是速率限制的; 以及通过分光光度法或色谱监测偶联的脱氨基反应产物，或随后与其偶联的酶或化学反应来确定甲基转移酶活性。次黄嘌呤与尿酸和过氧化氢的偶联氧化任选地使用纯化或重组的黄嘌呤氧化酶来影响，其中甲基转移酶活性是速率限制的，并且其中确定甲基转移酶活性包括监测偶联的氧化反应。公开的变化包括监测反应产物（例如，检测NH 3，次黄嘌呤，H 2 O 2和尿酸）。

3. 发明申请

US20060078906A1 Methods, compositions, and kits comprising linker probes for quantifying polynucleotides 有权
公开(公告)号：US20060078906A1
公开(公告)日：2006-04-13
申请号：US11142720
申请日：2005-05-31
申请人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Lao , Neil Straus
发明人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Lao , Neil Straus
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C07H21/04 , C12N9/00 , C12P19/34 , C12Q1/686 , C12Q2525/301 , C12Q2600/16 , C12Q2600/178
摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

4. 发明申请

US20130011840A1 Methods, Compositions, and Kits Comprising Linker Probes for Quantifying Polynucleotides 审中-公开
标题翻译：包含用于定量多核苷酸的接头探针的方法，组合物和试剂盒
公开(公告)号：US20130011840A1
公开(公告)日：2013-01-10
申请号：US13612485
申请日：2012-09-12
申请人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
发明人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C07H21/04 , C12N9/00 , C12P19/34 , C12Q1/686 , C12Q2525/301 , C12Q2600/16 , C12Q2600/178
摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.
摘要翻译：本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。然后可以在多个扩增反应中解码多个查询的miRNA。

5. 发明申请

US20100112573A1 Methods, Compositions, and Kits Comprising Linker Probes for Quantifying Polynucleotides 有权
标题翻译：包含用于定量多核苷酸的接头探针的方法，组合物和试剂盒
公开(公告)号：US20100112573A1
公开(公告)日：2010-05-06
申请号：US12543466
申请日：2009-08-18
申请人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
发明人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C07H21/04 , C12N9/00 , C12P19/34 , C12Q1/686 , C12Q2525/301 , C12Q2600/16 , C12Q2600/178
摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.
摘要翻译：本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。然后可以在多个扩增反应中解码多个查询的miRNA。

6. 发明申请

US20180259113A1 TWO-BRANCH OR MULTI-BRANCH DOUBLE-LAYER WEAR-AND-IMPACT-RESISTANT TUBE AND MANUFACTURING METHOD THEREOF 审中-公开
公开(公告)号：US20180259113A1
公开(公告)日：2018-09-13
申请号：US15557403
申请日：2016-03-10
申请人： Zhaohui ZHOU
发明人： Zhaohui ZHOU
IPC分类号： F16L57/06
CPC分类号： F16L57/06 , B65G53/32 , B65G53/523 , B65G2207/48 , F16L9/02 , F16L9/22 , F16L43/001 , F16L43/002 , F16L43/02
摘要： A two-branch or multi-branch double-layer wear-and-impact-resistant tube and manufacturing method thereof. The wear-and-impact-resistant tube comprises an outer protective tube and an inner wear-resistant assembled tube nested in the outer protective tube. The inner wear-resistant assembled tube comprises two or more tube walls, the two or more tube walls are jointed to form a tube having an annular cross section. The wear-and-impact-resistant tube can be a straight tube or a bent tube. If the wear-and-impact-resistant tube is a straight tube, upper and lower portions of the inner wear-resistant assembled tube employ different structures, and if the wear-and-impact-resistant tube is a bent tube, a large curved portion and a small curved portion of the bent tube employ different structures. The inner assembled tube of the wear-and-impact-resistant tube is divided into at least two portions, and the at least two portions thereof employ different structures. The tube structure extends a service life thereof, improves wear resistance and impact resistance thereof, and reduces a total weight of the tube.

7. 发明申请

US20170314724A1 AUTO-FILL TWO-LAYER TWO-HALF WEAR RESISTANT ELBOW OF CONCRETE PUMP TRUCK AND MANUFACTURING METHOD THEREOF 审中-公开
公开(公告)号：US20170314724A1
公开(公告)日：2017-11-02
申请号：US15523564
申请日：2016-01-28
申请人： Zhaohui ZHOU
发明人： Zhaohui ZHOU
IPC分类号： F16L57/06 , E04G21/04 , F16L9/19 , F16L43/00
CPC分类号： F16L57/06 , B65G53/523 , E04G21/0436 , F16L9/19 , F16L43/00 , F16L43/001
摘要： An auto-fill two-layer two-half wear resistant elbow of a concrete pump truck consists of an outer-layer protection elbow, an inner-layer heterogeneous wear resistant combined elbow and a wear resistant connecting flange. A filling space is provided between the outer-layer protection elbow and the inner-layer heterogeneous wear resistant combined elbow. The wear resistant elbow is designed as a two-layer two-half unique structure including a protection layer and a wear resistant layer, wherein the protection layer protects the wear resistant layer from distortion or even damage by external force and distortion or even damage by impact force by internal materials during the transportation, installation and use of the elbow. Upon using the wear resistant elbow for the first time, the concrete grout fills the reserved slit via the heterogenous combined elbow and enters the reserved filling space, allowing the outer-layer protection elbow and the inner-layer heterogeneous wear resistant combined elbow to be fixed as a whole, which may realize the purpose of reducing the weight of the entire elbow, reducing costs, saving materials and increasing service life.

8. 发明申请

US20140038241A1 GENOMIC ENRICHMENT METHOD, COMPOSITION, AND REAGENT KIT 审中-公开
标题翻译：基因组培养方法，组合物和试剂盒
公开(公告)号：US20140038241A1
公开(公告)日：2014-02-06
申请号：US13958553
申请日：2013-08-03
申请人： Zhaohui Zhou , Qun Shan
发明人： Zhaohui Zhou , Qun Shan
IPC分类号： C12P19/34 , C12N9/16
CPC分类号： C12P19/34 , C12N9/16 , C12N9/22 , C12Q1/6806 , C12Y301/21 , C12Q2521/301 , C12Q2521/543 , C12Q2522/101
摘要： By using engineered sequence specific DNA nuclease (“SSDN”), the composition, reagent kit and method of the present invention can cut and release a DNA sequence of interest 1×104-1×107-base pairs long from a source DNA as large as the whole genome. The SSDN further includes an affinity tag or is bound to a solid support that facilitates the isolation of the DNA sequence of interest. The SSDN can include a RecA and Ref combination, a transcription activator like effector nuclease, or a sequence specific chemical nuclease. When applied to genomic sequencing, specific region(s) of interest in the genome can be cut and isolated. Because the irrelevant part of the genome is removed from the sequencing reaction, the speed, cost, and accuracy of genomic sequencing can be improved.
摘要翻译：通过使用工程序列特异性DNA核酸酶（“SSDN”），本发明的组合物，试剂盒和方法可以从源DNA中切下并释放出1×104-1×107碱基对长的DNA序列作为全基因组。 SSDN还包括亲和标签或者结合固体支持物，其有利于分离所关注的DNA序列。 SSDN可以包括RecA和Ref组合，转录激活物如效应核酸酶或序列特异性化学核酸酶。当应用于基因组测序时，可以切割和分离基因组中感兴趣的特定区域。因为从测序反应中去除了基因组的不相关部分，因此可以提高基因组测序的速度，成本和准确性。

9. 发明授权

US08632975B2 Nucleotide transient binding for sequencing methods 有权
标题翻译：测序方法的核苷酸瞬变结合
公开(公告)号：US08632975B2
公开(公告)日：2014-01-21
申请号：US12790760
申请日：2010-05-28
申请人： Peter B. Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
发明人： Peter B. Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
摘要翻译：本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

10. 发明申请

US20120322676A1 COMPOSITIONS AND METHODS FOR DETECTION OF CRONOBACTER SPP. AND CRONOBACTER SPECIES AND STRAINS 审中-公开
标题翻译：用于检测CRONOBACTER SPP的组合物和方法。和克隆物种和菌株
公开(公告)号：US20120322676A1
公开(公告)日：2012-12-20
申请号：US13495748
申请日：2012-06-13
申请人： Yongmei JI , Sheung-Mei ("Rita") Shih , Lovorka Degoricija , Zhaohui Zhou , Craig Cummings , Manohar Furtado , Pius Brzoska , Angela Burrell , Harrison Leong , Xingwang Fang , Mangkey Bounpheng , Rohan Shah
发明人： Yongmei JI , Sheung-Mei ("Rita") Shih , Lovorka Degoricija , Zhaohui Zhou , Craig Cummings , Manohar Furtado , Pius Brzoska , Angela Burrell , Harrison Leong , Xingwang Fang , Mangkey Bounpheng , Rohan Shah
IPC分类号： C12Q1/68 , C40B30/04 , G01N21/76 , C07H21/04 , G01N27/62
CPC分类号： C12Q1/689 , C12R1/01
摘要： Disclosed are genomic sequences for nine strains of Cronobacter spp. (C. sakazakii—696, 701, 680; C. malonaticus—507, 681; C. turicensis—564; C. muytjensii—530; C. dublinensis—582; C. genomosp1—581) and compositions, methods, and kits for detecting, identifying and distinguishing Cronobacter spp. strains from each other and from non-Cronobacter spp. strains. Some embodiments describe isolated nucleic acid compositions unique to certain Cronobacter strains as well as compositions that are specific to all Cronobacter spp. Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of Cronobacter spp. are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR data.
摘要翻译：公开了九个克罗杆菌属菌株的基因组序列。（C.sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组sp1-581）和组合物，方法和试剂盒用于检测，识别和鉴别克罗杆菌属。菌株彼此和非克罗杆菌属。菌株。一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。还提供引物和探针组合物以及引物和探针的使用方法。克隆杆菌属鉴定试剂盒也被描述。一些实施例涉及用于设置用于PCR数据分析的基于控制的阈值的计算机软件方法。

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