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1. 发明授权

US08278051B2 Method of amplifying a target nucleic acid by rolling circle amplification 有权
标题翻译：通过滚圆放大扩增靶核酸的方法
公开(公告)号：US08278051B2
公开(公告)日：2012-10-02
申请号：US13185003
申请日：2011-07-18
申请人： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
发明人： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6855 , C12Q2525/301 , C12Q2531/125 , C12Q2537/101 , C12Q2521/301 , C12Q2521/501
摘要： Provided is a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase, (d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
摘要翻译：提供了使用滚动循环扩增（RCA）扩增核酸的方法，包括使用RCA形成单链环状DNA模板来扩增核酸，反应溶液包括：（a）两个发夹寡核苷酸（ b）靶核酸，（c）DNA连接酶，（d）内切核酸酶，（e）DNA聚合酶和（f）引物。

2. 发明授权

US08185324B2 Method of determining initial concentration of nucleic acid in sample using real-time amplification data 有权
标题翻译：使用实时放大数据确定样品中核酸初始浓度的方法
公开(公告)号：US08185324B2
公开(公告)日：2012-05-22
申请号：US12259548
申请日：2008-10-28
申请人： Kak Namkoong , Young-sun Lee , Chin-sung Park , Joo-won Rhee
发明人： Kak Namkoong , Young-sun Lee , Chin-sung Park , Joo-won Rhee
IPC分类号： G01N33/48
CPC分类号： C12Q1/6851 , C12Q2537/165
摘要： Disclosed is a method of determining an initial concentration of a target nucleic acid within a sample using real-time nucleic acid amplification data. Amplification efficiencies of the target nucleic acid with respect to amplification time are obtained from signals of amplified products, and an amplification efficiency function with respect to amplification time is formulated employing the amplification efficiencies.
摘要翻译：公开了使用实时核酸扩增数据确定样品中靶核酸的初始浓度的方法。从放大产物的信号获得靶核酸相对于扩增时间的扩增效率，并且使用扩增效率来制定关于扩增时间的扩增效率函数。

3. 发明授权

US08003322B2 Method of amplifying a target nucleic acid by rolling circle amplification 有权
标题翻译：通过滚圆放大扩增靶核酸的方法
公开(公告)号：US08003322B2
公开(公告)日：2011-08-23
申请号：US12247433
申请日：2008-10-08
申请人： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
发明人： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6855 , C12Q2525/301 , C12Q2531/125 , C12Q2537/101 , C12Q2521/301 , C12Q2521/501
摘要： Provided is kit for and a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase,(d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
摘要翻译：提供了使用滚动循环扩增（RCA）扩增核酸的试剂盒和使用RCA形成单链环状DNA模板的核酸的方法，所述反应溶液包括：（a）两个发夹寡核苷酸，（b）靶核酸，（c）DNA连接酶，（d）内切核酸酶，（e）DNA聚合酶和（f）引物。

4. 发明授权

US08136550B2 Elastic valve and microfluidic device including the same 有权
标题翻译：包括弹性阀和微流体装置
公开(公告)号：US08136550B2
公开(公告)日：2012-03-20
申请号：US12023197
申请日：2008-01-31
申请人： Kak Namkoong , Chin-sung Park , Jin-tae Kim , Joo-won Rhee
发明人： Kak Namkoong , Chin-sung Park , Jin-tae Kim , Joo-won Rhee
IPC分类号： E03B1/00
CPC分类号： F16K99/0001 , Y10T137/87434 , Y10T137/877
摘要： An elastic valve and a microfluidic device including the same are provided. The elastic valve includes a channel dented by external pressure so as to be elastically restituted; and a plurality of channel closing protrusions, which comprise elastomer as a material, which are protruded from at least one inner side surface of the channel toward an opposite inner side surface, and which are separated from each other so as not to interrupt a flow of fluids, wherein, when external pressure is applied in order to dent the channel, the plurality of channel closing protrusions are deformed so as to be elastically restituted, thereby closing the channel.
摘要翻译：提供弹性阀和包括该弹性阀的微流体装置。弹性阀包括通过外部压力凹陷以便被弹性恢复的通道; 以及多个通道闭合突起，其包括弹性体作为材料，其从所述通道的至少一个内侧表面突出到相对的内侧表面，并且彼此分离，以便不会中断流动流体，其中当施加外部压力以凹陷通道时，多个通道关闭突起变形以便被弹性地恢复，从而关闭通道。

5. 发明授权

US07838300B2 Method of reducing temperature difference between a pair of substrates 有权
标题翻译：降低一对基板之间的温度差的方法
公开(公告)号：US07838300B2
公开(公告)日：2010-11-23
申请号：US11689640
申请日：2007-03-22
申请人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
发明人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
IPC分类号： G01N25/20 , B01L3/02
CPC分类号： B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
摘要： A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer.
摘要翻译：降低高温和低温基板之间的温度差的方法包括：介于热传导率高于空气的传热促进层，并且可以在基板之间保持颗粒，并且保持高温基板，传热促进层和低温基板，其中至少可以在高温基板和传热促进层之间以及在低温基板和热量之间基本上防止空气层的形成转移促进层。

6. 发明授权

US08771610B2 Method of reducing temperature difference between a pair of substrates and fluid reaction device using the same 失效
标题翻译：降低一对基板与使用其的液体反应装置之间的温度差的方法
公开(公告)号：US08771610B2
公开(公告)日：2014-07-08
申请号：US12904557
申请日：2010-10-14
申请人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
发明人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
IPC分类号： B01L3/00
CPC分类号： B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
摘要： A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.
摘要翻译：流体反应装置包括容纳流体的微流体反应芯片，加热器和介于微流体反应芯片和加热器之间的传热促进层，传热促进层具有比空气更高的导热性并且可以保持颗粒，其中可以防止空气层的形成。

7. 发明申请

US20080053647A1 METHOD OF REDUCING TEMPERATURE DIFFERENCE BETWEEN A PAIR OF SUBSTRATES AND FLUID REACTION DEVICE USING THE SAME 有权
标题翻译：降低基板对流与使用其的流体反应装置之间的温度差异的方法
公开(公告)号：US20080053647A1
公开(公告)日：2008-03-06
申请号：US11689640
申请日：2007-03-22
申请人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
发明人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
IPC分类号： F28F7/00
CPC分类号： B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
摘要： A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer. A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.
摘要翻译：降低高温和低温基板之间的温度差的方法包括：介于热传导率高于空气的传热促进层，并且可以在基板之间保持颗粒，并且保持高温基板，传热促进层和低温基板，其中至少可以在高温基板和传热促进层之间以及在低温基板和热量之间基本上防止空气层的形成转移促进层。流体反应装置包括容纳流体的微流体反应芯片，加热器和介于微流体反应芯片和加热器之间的传热促进层，传热促进层具有比空气更高的导热性并且可以保持颗粒，其中可以防止空气层的形成。

8. 发明申请

US20090142798A1 METHOD OF SELECTIVELY LYSING NON-VIABLE CELLS IN CELL POPULATION IN SAMPLE 审中-公开
标题翻译：在样品中细胞群体中选择性选择不可见细胞的方法
公开(公告)号：US20090142798A1
公开(公告)日：2009-06-04
申请号：US12108586
申请日：2008-04-24
申请人： Young-sun LEE , Myo-yong Lee , Soo-hyung Choi , Kak Namkoong , Chin-sung Park
发明人： Young-sun LEE , Myo-yong Lee , Soo-hyung Choi , Kak Namkoong , Chin-sung Park
IPC分类号： G01N33/48 , C12N1/06
CPC分类号： C12Q1/22 , C12N1/06 , G01N2333/205
摘要： The present invention provides a method of selectively lysing non-viable cells from a cell population within a sample, the method including selectively lysing non-viable cells by mixing a cell-containing sample with a lysis buffer including a non-ionic surfactant and a divalent cation salt.
摘要翻译：本发明提供从样品中的细胞群选择性裂解非存活细胞的方法，所述方法包括通过将包含细胞的样品与包含非离子表面活性剂的裂解缓冲液和二价阳离子盐。

9. 发明授权

US07790109B2 Micro-fluid reaction vessel, method for manufacturing the same, and micro-fluid reaction method using the vessel 有权
标题翻译：微流体反应容器，其制造方法和使用该容器的微流体反应方法
公开(公告)号：US07790109B2
公开(公告)日：2010-09-07
申请号：US11625877
申请日：2007-01-23
申请人： Su-hyeon Kim , Jin-tae Kim , Kak Namkoong , In-ho Lee , Chin-sung Park , Young-sun Lee
发明人： Su-hyeon Kim , Jin-tae Kim , Kak Namkoong , In-ho Lee , Chin-sung Park , Young-sun Lee
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , B01J10/00 , B01J10/02 , B01J12/00 , B01J12/02 , F15C1/04 , F15C3/00 , F15C1/06
CPC分类号： B01L3/502738 , B01L7/52 , B01L2200/0689 , B01L2300/0816 , B01L2300/0825 , B01L2300/0887 , B01L2300/123 , B01L2400/0655 , B29C33/3857 , B29C33/60 , Y10T137/218 , Y10T137/2202 , Y10T137/2224
摘要： A micro-fluid reaction vessel includes an upper plate formed of an elastomer, a lower plate adhered to the upper plate, a micro-chamber and a micro-channel formed on an inner surface of the upper plate facing the lower plate and an inlet hole and an outlet hole formed in the upper plate and through which a fluid flows into or out of, respectively. The micro-channel is constructed to be closed by pressure applied to the upper plate and elastically restored when the pressure is not applied. A micro fluid reaction method uses the micro fluid reaction vessel and a method of manufacturing forms the microfluid reaction vessel.
摘要翻译：微流体反应容器包括由弹性体形成的上板，粘附到上板的下板，形成在面向下板的上板的内表面上的微室和微通道，以及入口孔以及形成在上板中并且流体分别流入或流出的出口孔。微通道被构造为通过施加到上板的压力而关闭，并且当不施加压力时弹性恢复。微流体反应方法使用微流体反应容器和制造方法形成微流体反应容器。

10. 发明授权

US07767439B2 Real-time PCR monitoring apparatus and method 有权
标题翻译：实时PCR监测装置及方法
公开(公告)号：US07767439B2
公开(公告)日：2010-08-03
申请号：US10890350
申请日：2004-07-13
申请人： Kwang-wook Oh , Jin-tae Kim , Kak Namkoong , Chin-sung Park
发明人： Kwang-wook Oh , Jin-tae Kim , Kak Namkoong , Chin-sung Park
IPC分类号： C12M1/34 , C12M3/00 , C12Q1/68 , G01N21/25
CPC分类号： G01N21/645 , B01L3/50851 , B01L7/52 , B01L2300/0627 , B01L2300/18 , G01N21/0332
摘要： A real time polymerase chain reaction (“PCR”) monitoring apparatus includes, a microchip-type PCR tube that has a PCR solution-containing PCR chamber, a micro-heater, a detection unit detecting a PCR product signal based on the PCR solution, a plurality of modules, each of which includes the abovementioned elements in addition to a cooling fan and a control unit controlling the micro-heater and the cooling fan to adjust the temperature of the PCR chamber, a base instrument that comprises a power supply unit connected to the modules and a data communication unit connected to the control unit of each of the modules, and a display unit displaying data from the data communication unit, wherein the control unit of each of the modules independently controls at least one of both the detection unit and the temperature of the PCR chamber of the PCR tube in each of the modules.
摘要翻译：实时聚合酶链式反应（“PCR”）监测装置包括：具有含PCR溶液的PCR室的微芯片型PCR管，微加热器，基于PCR溶液检测PCR产物信号的检测单元，多个模块，每个模块除了冷却风扇之外还包括上述元件，以及控制单元，其控制微加热器和冷却风扇来调节PCR室的温度;基础仪器，包括连接的电源单元连接到每个模块的控制单元的数据通信单元和从数据通信单元显示数据的显示单元，其中每个模块的控制单元独立地控制检测单元中的至少一个以及每个模块中PCR管的PCR室的温度。

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