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1. 发明申请

US20090269822A1 MICROORGANISM 有权
标题翻译：微生物
公开(公告)号：US20090269822A1
公开(公告)日：2009-10-29
申请号：US12499362
申请日：2009-07-08
申请人： Jonathan Hughes , Yvonne Armitage , Jatinder Kullar , Stuart Greenhalgh
发明人： Jonathan Hughes , Yvonne Armitage , Jatinder Kullar , Stuart Greenhalgh
IPC分类号： C12P13/02 , C12N9/88
CPC分类号： C12R1/01 , C12N1/04 , C12N1/20 , C12N9/88 , C12P13/02 , Y10S435/822
摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrile hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrile wherein the nitrile is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NCIMB 41164, a mutant thereof and a nitrile hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NCIMB 41164.
摘要翻译：作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。要求从微生物获得的腈水合酶。还要求的是从相应的腈制备酰胺的方法，其中腈在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NCIMB 41164，其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的腈水合酶或其突变体。还要求保存红玫瑰色红球菌NCIMB 41164的方法。

2. 发明授权

US07575912B2 Strain-of Rhodococcus rhodochrous NCIMB 41164 and its use as producer of nitrile hydratase 有权
标题翻译：菌株Rhodococcus rhodochrous NCIMB 41164及其用作腈水合酶的生产者
公开(公告)号：US07575912B2
公开(公告)日：2009-08-18
申请号：US10580448
申请日：2004-11-22
申请人： Jonathan Hughes , Yvonne Armitage , Jatinder Kullar , Stuart Greenhalgh
发明人： Jonathan Hughes , Yvonne Armitage , Jatinder Kullar , Stuart Greenhalgh
IPC分类号： C12N1/20 , C12N1/00
CPC分类号： C12R1/01 , C12N1/04 , C12N1/20 , C12N9/88 , C12P13/02 , Y10S435/822
摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrite hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrite wherein the nitrite is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NUMB 41164, a mutant thereof and a nitrite hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NUMB 41164.
摘要翻译：作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。要求从微生物获得的亚硝酸盐水合酶。还要求的是从相应的亚硝酸盐制备酰胺的方法，其中亚硝酸盐在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NUMB 41164，其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的亚硝酸盐水合酶或其突变体。还要求保存红玫瑰色红球菌NUMB 41164的方法。

3. 发明申请

US20070077634A1 Microorganism 有权
标题翻译：微生物
公开(公告)号：US20070077634A1
公开(公告)日：2007-04-05
申请号：US10580448
申请日：2004-11-22
申请人： Jonathan Hughes , Yvonne Armitage , Jatinder Kullar , Stuart Greenhalgh
发明人： Jonathan Hughes , Yvonne Armitage , Jatinder Kullar , Stuart Greenhalgh
IPC分类号： C07H21/04 , C12P13/02 , C12N9/78 , C12N15/74 , C12N1/21
CPC分类号： C12R1/01 , C12N1/04 , C12N1/20 , C12N9/88 , C12P13/02 , Y10S435/822
摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrite hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrite wherein the nitrite is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NUMB 41164, a mutant thereof and a nitrite hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NUMB 41164.
摘要翻译：作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。要求从微生物获得的亚硝酸盐水合酶。还要求的是从相应的亚硝酸盐制备酰胺的方法，其中亚硝酸盐在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NUMB 41164，其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的亚硝酸盐水合酶或其突变体。还要求保存红玫瑰色红球菌NUMB 41164的方法。

4. 发明授权

US08153406B2 Microorganism 有权
标题翻译：微生物
公开(公告)号：US08153406B2
公开(公告)日：2012-04-10
申请号：US12499362
申请日：2009-07-08
申请人： Jonathan Hughes , Yvonne Armitage , Jatinder Kullar , Stuart Greenhalgh
发明人： Jonathan Hughes , Yvonne Armitage , Jatinder Kullar , Stuart Greenhalgh
IPC分类号： C12P13/02 , C12P1/04 , C12N1/04 , C12N1/20 , C12N1/00
CPC分类号： C12R1/01 , C12N1/04 , C12N1/20 , C12N9/88 , C12P13/02 , Y10S435/822
摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrile hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrile wherein the nitrile is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NCIMB 41164, a mutant thereof and a nitrile hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NCIMB 41164.
摘要翻译：作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。要求从微生物获得的腈水合酶。还要求的是从相应的腈制备酰胺的方法，其中腈在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NCIMB 41164，其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的腈水合酶或其突变体。还要求保存红玫瑰色红球菌NCIMB 41164的方法。

5. 发明申请

US20070184536A1 Process of producing polymers 有权
标题翻译：生产聚合物的方法
公开(公告)号：US20070184536A1
公开(公告)日：2007-08-09
申请号：US10580447
申请日：2004-11-22
申请人： Stuart Greenhalgh , Kenneth Symes , Yvonne Armitage , Jonathan Hughes , Gary Richardson
发明人： Stuart Greenhalgh , Kenneth Symes , Yvonne Armitage , Jonathan Hughes , Gary Richardson
IPC分类号： C12P13/02 , C08F220/06
CPC分类号： C12P13/02 , C12P7/40 , C12P7/46
摘要： A process for preparing a polymer of an ethylenically unsaturated monomer, in which the monomer is obtainable from a biocatalysed reaction or a fermentation process, and wherein the monomer contains cellular material and/or components of a fermentation broth, forming the polymer by polymerising the ethylenically unsaturated monomer or a monomer mixture comprising the ethylenically unsaturated monomer, wherein there is substantially no removal of the cellular material and/or components of the fermentation broth from the ethylenically unsaturated monomer.
摘要翻译：一种制备烯键式不饱和单体的聚合物的方法，其中单体可从生物催化反应或发酵方法获得，并且其中单体含有发酵液的细胞材料和/或组分，通过聚合烯属不饱和单体形成聚合物不饱和单体或包含烯键式不饱和单体的单体混合物，其中基本上不从烯属不饱和单体中除去发酵液的细胞材料和/或组分。

6. 发明授权

US08067215B2 Process of producing polymers 有权
标题翻译：生产聚合物的方法
公开(公告)号：US08067215B2
公开(公告)日：2011-11-29
申请号：US10580447
申请日：2004-11-22
申请人： Stuart Greenhalgh , Kenneth Charles Symes , Yvonne Armitage , Jonathan Hughes , Gary Richardson
发明人： Stuart Greenhalgh , Kenneth Charles Symes , Yvonne Armitage , Jonathan Hughes , Gary Richardson
IPC分类号： C12P5/02 , C12P1/00 , C12P7/62 , C12P7/40 , C12P5/00
CPC分类号： C12P13/02 , C12P7/40 , C12P7/46
摘要： A process for preparing a polymer of an ethylenically unsaturated monomer, in which the monomer is obtainable from a biocatalysed reaction or a fermentation process, and wherein the monomer contains cellular material and/or components of a fermentation broth, forming the polymer by polymerizing the ethylenically unsaturated monomer or a monomer mixture comprising the ethylenically unsaturated monomer, wherein there is substantially no removal of the cellular material and/or components of the fermentation broth from the ethylenically unsaturated monomer.
摘要翻译：一种制备烯键式不饱和单体的聚合物的方法，其中单体可从生物催化反应或发酵方法获得，并且其中所述单体含有发酵液的细胞材料和/或组分，通过聚合烯属不饱和单体形成聚合物不饱和单体或包含烯键式不饱和单体的单体混合物，其中基本上不从烯属不饱和单体中除去发酵液的细胞材料和/或组分。

7. 发明申请

US20070077633A1 Manufacture of amides 有权
标题翻译：制造酰胺
公开(公告)号：US20070077633A1
公开(公告)日：2007-04-05
申请号：US10580446
申请日：2004-11-22
申请人： Yvonne Armitage , Jatinder Kullar
发明人： Yvonne Armitage , Jatinder Kullar
IPC分类号： C12P13/02 , C12N1/20
CPC分类号： C12P13/02 , Y10S435/822
摘要： A method of producing an amide from the corresponding nitrile comprising the following steps, i) providing a microorganism capable of producing a nitrile hydratase biocatalyst, ii) culturing the microorganism in a growth medium, iii) storing the microorganism, iv) contacting the nitrile by the microorganism in an aqueous medium and thereby converting the nitrile to the amide, wherein the microorganism is stored as none actively growing free cells in a storage medium that comprises water. The stored microorganism may be as whole microbial cells, this may be in the form of a cell paste recovered from a fermentation medium; an aqueous suspension of the microbial cells, prepared using a suitable suspending medium such as water, physiological saline solution, or a suitable buffer solution, such as phosphate or comprises the fermentation broth that contains components of the fermentation culture medium and products of microbial culture. The microorganism exhibits no significant loss of activity, for example if stored even for at least 2 days, especially 3 to 28 days.
摘要翻译：一种从相应的腈生产酰胺的方法，包括以下步骤：i）提供能够产生腈水合酶生物催化剂的微生物，ii）在生长培养基中培养微生物，iii）储存微生物，iv）使腈接触微生物在水性介质中，从而将腈转化为酰胺，其中微生物作为无活性生长的游离细胞储存在包含水的储存培养基中。储存的微生物可以是整个微生物细胞，其可以是从发酵培养基中回收的细胞糊的形式; 使用合适的悬浮介质如水，生理盐水或适当的缓冲溶液（例如磷酸盐）制备的微生物细胞的水性悬浮液，或包含含有发酵培养基的组分和微生物培养产物的发酵液。微生物显示出不显着的活性损失，例如如果储存至少2天，特别是3至28天。

8. 发明授权

US07592164B2 Process for preparing unsaturated amides and carboxylic acids 失效
标题翻译：制备不饱和酰胺和羧酸的方法
公开(公告)号：US07592164B2
公开(公告)日：2009-09-22
申请号：US10580445
申请日：2004-11-22
申请人： Jonathan Hughes , Yvonne Armitage
发明人： Jonathan Hughes , Yvonne Armitage
IPC分类号： C12P13/02 , C12P1/02 , C12N1/20
CPC分类号： C12R1/01 , C12N9/80 , C12N9/88 , C12P7/40 , C12P13/02 , C12Y305/01004 , C12Y402/01084 , Y10S435/822
摘要： A process of producing an amide from a nitrile by the action of a nitrile hydratase enzyme or ammonium salt of an ethylenically unsaturated carboxylic acid from an amide by the action of amidase respectively in which enzymes are obtainable from a microorganism of the Dietzia genus.
摘要翻译：通过腈水解酶或烯属不饱和羧酸的铵盐从酰胺通过酰胺酶的作用从腈制备酰胺的方法，其中酶可以从杜氏弧菌属的微生物获得。

9. 发明申请

US20070184535A1 Process for preparing unsaturated amides and carboxylic acids 失效
标题翻译：制备不饱和酰胺和羧酸的方法
公开(公告)号：US20070184535A1
公开(公告)日：2007-08-09
申请号：US10580445
申请日：2004-11-22
申请人： Jonathan Hughes , Yvonne Armitage
发明人： Jonathan Hughes , Yvonne Armitage
IPC分类号： C12P13/02 , C12N9/80 , C12N1/20
CPC分类号： C12R1/01 , C12N9/80 , C12N9/88 , C12P7/40 , C12P13/02 , C12Y305/01004 , C12Y402/01084 , Y10S435/822
摘要： A process of producing an amide from a nitrile by the action of a nitrile hydratase enzyme or ammonium salt of an ethylenically unsaturated carboxylic acid from an amide by the action of amidase respectively in which enzymes are obtainable from a microorganism of the Dietzia genus.
摘要翻译：通过腈水解酶或烯属不饱和羧酸的铵盐从酰胺通过酰胺酶的作用从腈制备酰胺的方法，其中酶可以从杜氏弧菌属的微生物获得。

10. 发明授权

US09434969B2 Manufacture of amides 有权
标题翻译：制造酰胺
公开(公告)号：US09434969B2
公开(公告)日：2016-09-06
申请号：US12879558
申请日：2010-09-10
申请人： Yvonne Armitage , Jatinder Singh Kullar
发明人： Yvonne Armitage , Jatinder Singh Kullar
IPC分类号： C12P13/02
CPC分类号： C12P13/02 , Y10S435/822
摘要： A method of producing an amide from the corresponding nitrile comprising the following steps, i) providing a microorganism capable of producing a nitrile hydratase biocatalyst, ii) culturing the microorganism in a growth medium, iii) storing the microorganism, iv) contacting the nitrile by the microorganism in an aqueous medium and thereby converting the nitrile to the amide, wherein the microorganism is stored as none actively growing free cells in a storage medium that comprises water. The stored microorganism may be as whole microbial cells, this may be in the form of a cell paste recovered from a fermentation medium; an aqueous suspension of the microbial cells, prepared using a suitable suspending medium such as water, physiological saline solution, or a suitable buffer solution, such as phosphate or comprises the fermentation broth that contains components of the fermentation culture medium and products of microbial culture. The microorganism exhibits no significant loss of activity, for example if stored even for at least 2 days, especially 3 to 28 days.

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