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1. 发明申请

US20100028892A1 YEAST BASED EXPRESSION OF PROTEASES AND METHODS OF USE 审中-公开
标题翻译：基于YEAST的表达及其使用方法
公开(公告)号：US20100028892A1
公开(公告)日：2010-02-04
申请号：US12504600
申请日：2009-07-16
申请人： John C. Reed , Hideki Hayashi , Michael Cuddy
发明人： John C. Reed , Hideki Hayashi , Michael Cuddy
IPC分类号： C12Q1/68
CPC分类号： C12N15/1055 , C12N15/1086 , C12N15/81
摘要： This disclosure generally relates to components and methods of using a high throughput screening (HTS) systems for intracellular proteases, using Caspases as a prototype. Genetic systems are disclosed for monitoring exogenous caspase activation pathways in the yeast, Saccharomyces cerevisiae. The yeast-based cellular systems permit facile expression of proteases (e.g., caspase) and protease-activating proteins in combinations that reconstitute entire mammalian pathways in these simple eukaryotes. Among the assay methods integrated into the yeast system are cleavable reporter gene activators, in which protease-mediated cleavage activates a transcription factor. Exemplary systems rely, singly or in concert, on exogenous recombinant caspases and exogenous upstream activators of caspases to cleave a chimeric protein giving rise to a transcription factor which induces the expression of the LacZ and LEU2 genes. The activities of these genes result in colored cultures and impart the ability of the yeast to grow in leucine deficient media. The intensity of the color is measured by colorimetry and quantified with OD units. The OD units are directly proportional to the activity of the caspase in the system. The method of quantification is referred to as the “readout”.
摘要翻译：本公开通常涉及使用胱天蛋白酶作为原型的细胞内蛋白酶的高通量筛选（HTS）系统的组件和方法。公开了用于监测酵母，酿酒酵母中的外源半胱天冬酶活化途径的遗传系统。基于酵母的细胞系统允许在这些简单真核生物中重组整个哺乳动物途径的组合，容易地表达蛋白酶（例如胱天蛋白酶）和蛋白酶活化蛋白。整合到酵母系统中的测定方法中是可裂解的报告基因激活因子，其中蛋白酶介导的切割激活转录因子。示例性系统单独或一致地依赖于外源重组胱天蛋白酶和胱天蛋白酶的外源上游激活剂以切割嵌合蛋白，产生诱导LacZ和LEU2基因表达的转录因子。这些基因的活性导致有色培养物，并赋予酵母在亮氨酸缺乏培养基中生长的能力。通过比色法测量颜色的强度并用OD单位定量。 OD单位与系统中半胱天冬酶的活性成正比。定量方法被称为“读出”。

2. 发明授权

US07626001B2 Card domain containing polypeptides, encoding nucleic acids, and methods of use 失效
公开(公告)号：US07626001B2
公开(公告)日：2009-12-01
申请号：US11728892
申请日：2007-03-26
申请人： John C. Reed , Frederick F. Pio , Adam Godzik , Christian Stehlik , Jason S. Damiano , Sug Hyung Lee , Vasco A. Oliveira , Hideki Hayashi , Kryzysztof Pawlowski
发明人： John C. Reed , Frederick F. Pio , Adam Godzik , Christian Stehlik , Jason S. Damiano , Sug Hyung Lee , Vasco A. Oliveira , Hideki Hayashi , Kryzysztof Pawlowski
IPC分类号： C07K14/00
CPC分类号： G06F17/3089 , C07K14/4702 , C07K2319/00 , G06F17/30699
摘要： The invention provides caspase recruitment domain (CARD)-containing polypeptides, CARD, NB-ARC, ANGIO-R, LRR and SAM domains therefrom, as well as encoding nucleic acid molecules and specific antibodies. The invention also provides related screening, diagnostic and therapeutic methods.

3. 发明申请

US20110008319A1 CARD DOMAIN CONTAINING POLYPEPTIDES, ENCODING NUCLEIC ACIDS, AND METHODS OF USE 审中-公开
标题翻译：含有多核苷酸的多核苷酸，编码核酸的方法和使用方法
公开(公告)号：US20110008319A1
公开(公告)日：2011-01-13
申请号：US12628136
申请日：2009-11-30
申请人： John C. Reed , Frederick F. Pio , Adam Godzik , Christian Stehlik , Jason S. Damiano , Sug Hyung Lee , Vasco A. Oliveira , Hideki Hayashi , Kryzysztof Pawlowski
发明人： John C. Reed , Frederick F. Pio , Adam Godzik , Christian Stehlik , Jason S. Damiano , Sug Hyung Lee , Vasco A. Oliveira , Hideki Hayashi , Kryzysztof Pawlowski
IPC分类号： A61K39/395 , C07H21/04 , C07K14/00 , C07K7/06 , A61P35/00 , C12N15/63 , C12N5/10
CPC分类号： G06F16/958 , C07K14/4702 , C07K2319/00 , G06F16/335
摘要： The invention provides caspase recruitment domain (CARD)-containing polypeptides, CARD, NB-ARC, ANGIO-R, LRR and SAM domains therefrom, as well as encoding nucleic acid molecules and specific antibodies. The invention also provides related screening, diagnostic and therapeutic methods.
摘要翻译：本发明提供了含有胱天蛋白酶募集区（CARD）的多肽，CARD，NB-ARC，ANGIO-R，LRR和SAM结构域，以及编码核酸分子和特异性抗体。本发明还提供了相关的筛选，诊断和治疗方法。

4. 发明授权

US07196182B2 Card domain containing polypeptides, encoding nucleic acids, and methods of use 失效
标题翻译：包含多肽的卡结构域，编码核酸和使用方法
公开(公告)号：US07196182B2
公开(公告)日：2007-03-27
申请号：US09864921
申请日：2001-05-23
申请人： John C. Reed , Frederick F. Pio , Adam Godzik , Christian Stehlik , Jason S. Damiano , Sug Hyung Lee , Vasco A. Oliveira , Hideki Hayashi , Krzysztof Pawlowski
发明人： John C. Reed , Frederick F. Pio , Adam Godzik , Christian Stehlik , Jason S. Damiano , Sug Hyung Lee , Vasco A. Oliveira , Hideki Hayashi , Krzysztof Pawlowski
IPC分类号： C07H21/02 , C12Q1/68
CPC分类号： G06F17/3089 , C07K14/4702 , C07K2319/00 , G06F17/30699
摘要： The invention provides caspase recruitment domain (CARD)-containing polypeptides, CARD, NB-ARC, ANGIO-R, LRR and SAM domains therefrom, as well as encoding nucleic acid molecules and specific antibodies. The invention also provides related screening, diagnostic and therapeutic methods.
摘要翻译：本发明提供了含有胱天蛋白酶募集区（CARD）的多肽，CARD，NB-ARC，ANGIO-R，LRR和SAM结构域，以及编码核酸分子和特异性抗体。本发明还提供了相关的筛选，诊断和治疗方法。

5. 外观设计

USD880362S1 Motor vehicle and/or toy replica thereof 有权
公开(公告)号：USD880362S1
公开(公告)日：2020-04-07
申请号：US29673986
申请日：2018-12-19
申请人： Hideki Hayashi , Masayuki Sugiura
设计人： Hideki Hayashi , Masayuki Sugiura
摘要： FIG. 1 is a left, front perspective view of a motor vehicle and/or toy replica showing our new design;
FIG. 2 is a left, rear perspective view thereof;
FIG. 3 is a right, front perspective view thereof;
FIG. 4 is a right, rear perspective view thereof;
FIG. 5 is a front view thereof;
FIG. 6 is a rear view thereof;
FIG. 7 is a left side view thereof;
FIG. 8 is a right side view thereof; and,
FIG. 9 is a top view thereof.

6. 发明申请

US20140219924A1 MEDICAL TISSUE-MARKER AND MANUFACTURING METHOD FOR THE SAME 有权
标题翻译：医用组织标记及其制造方法
公开(公告)号：US20140219924A1
公开(公告)日：2014-08-07
申请号：US14126034
申请日：2012-05-31
申请人： Hideki Hayashi , Hiroya Hatakeyama , Masanori Fujinami , Taro Toyota
发明人： Hideki Hayashi , Hiroya Hatakeyama , Masanori Fujinami , Taro Toyota
IPC分类号： A61K49/00 , A61K49/04
CPC分类号： A61K49/0034 , A61K49/0002 , A61K49/0023 , A61K49/006 , A61K49/0084 , A61K49/0438 , A61K49/0461 , A61K49/0466
摘要： The present invention is to provide a medical tissue-marker which enables the identification of a location even from the outside of an organ, can remain topical over a long period, and enables the easy identification of a marked location within the whole organ; also to provide a manufacturing method for the medical tissue-marker. The medical tissue-marker as in the present invention includes a vesicle formed by the synthesis of a phospholipid and a near infrared fluorescent dye, and an emulsion formed by the synthesis of the phospholipid and an X-ray contrast medium, and has agglomerated clusters wherein the vesicle and the emulsion are contained in a hydrophilic solvent and a plurality of capsules are formed by means of an emulsifier.
摘要翻译：本发明提供一种能够从器官外部识别位置的医疗组织标记物，能够长时间保持局部，能够容易地识别整个器官内的标记位置; 也提供了用于医疗组织标记物的制造方法。本发明中的医疗用组织标记物包括通过合成磷脂和近红外荧光染料形成的囊泡，以及通过合成磷脂和X射线造影剂形成的乳液，并且具有团聚体，其中，囊泡和乳液包含在亲水溶剂中，并且通过乳化剂形成多个胶囊。

7. 发明授权

US08766278B2 Silicon carbide semiconductor device 有权
标题翻译：碳化硅半导体器件
公开(公告)号：US08766278B2
公开(公告)日：2014-07-01
申请号：US13565388
申请日：2012-08-02
申请人： Hideki Hayashi
发明人： Hideki Hayashi
IPC分类号： H01L29/15 , H01L21/8238
CPC分类号： H01L27/088 , H01L21/8213
摘要： First, second, fourth, and fifth impurity regions have a first conductivity type, and a third impurity region has a second conductivity type. The first to third impurity regions reach a first layer having the first conductivity type. The fourth and fifth impurity regions are provided on a second layer. First to fifth electrodes are provided on the first to fifth impurity regions, respectively. Electrical connection is established between the first and fifth electrodes, and between the third and fourth electrodes. A sixth electrode is provided on a gate insulating film covering a portion between the fourth and fifth impurity regions.
摘要翻译：第一，第二，第四和第五杂质区具有第一导电类型，第三杂质区具有第二导电类型。第一至第三杂质区域到达具有第一导电类型的第一层。第四和第五杂质区设置在第二层上。第一至第五电极分别设置在第一至第五杂质区上。在第一和第五电极之间以及第三和第四电极之间建立电连接。第六电极设置在覆盖第四和第五杂质区域之间的部分的栅极绝缘膜上。

8. 发明授权

US08618823B2 Semiconductor device 失效
标题翻译：半导体器件
公开(公告)号：US08618823B2
公开(公告)日：2013-12-31
申请号：US13006325
申请日：2011-01-13
申请人： Kenichi Tada , Koki Tsutsumida , Masatoshi Kawashima , Hideki Hayashi , Tsutomu Sato , Koichi Sugimoto
发明人： Kenichi Tada , Koki Tsutsumida , Masatoshi Kawashima , Hideki Hayashi , Tsutomu Sato , Koichi Sugimoto
IPC分类号： G01R31/28
CPC分类号： G01R31/318594 , G01R31/318552
摘要： A semiconductor device is designed to facilitate analyzing a position and a cause of the failure of an integrated circuit adopting a polyphase clock. To this end, the semiconductor device is provided with an error detecting unit that detects that a problem of the operation occurs in the integrated circuit, a clock state holding unit that holds the information of phases in a predetermined term of a two- or more-phase clock and an output unit that outputs the information of the phases in the predetermined term of the two- or more-phase clock when the error detecting unit detects that the problem of the operation occurs in the integrated circuit.
摘要翻译：半导体器件被设计为便于分析采用多相时钟的集成电路的故障的位置和原因。为此，半导体器件设置有检测在集成电路中发生操作问题的错误检测单元，时钟状态保持单元，其保持预定项中的相位信息为两维或多次，相位时钟和输出单元，当错误检测单元检测到集成电路中发生操作的问题时，输出两相或更多相位时钟的预定项中的相位的信息。

9. 外观设计

USD693497S1 Combination lamp for an automobile 有权
公开(公告)号：USD693497S1
公开(公告)日：2013-11-12
申请号：US29429216
申请日：2012-08-08
申请人： Hideki Hayashi
设计人： Hideki Hayashi

10. 发明授权

US08424839B2 Direct-current motor control device and method for detecting state of direct-current motor 失效
标题翻译：直流马达控制装置及直流马达状态检测方法
公开(公告)号：US08424839B2
公开(公告)日：2013-04-23
申请号：US13020207
申请日：2011-02-03
申请人： Hideki Hayashi , Satoru Hiramoto
发明人： Hideki Hayashi , Satoru Hiramoto
IPC分类号： F16K31/02
CPC分类号： H02K23/66 , F02D41/005 , H02P7/03 , Y02T10/47
摘要： A current detection unit detects an electric current caused by superimposing a direct current from a direct-current power source on an alternating current from an alternating-current power source and supplied through a brush to a direct-current motor. An extracting unit extracts an alternating-current component of the detected electric current. An angle detection unit detects a rotation angle of the motor according to the extracted alternating-current component. A direction detection unit detects a rotative direction of the motor according to a change pattern of the extracted alternating-current component. A core of the motor has slots each defined between adjacent two of teeth. The slots respectively accommodate phase coils respectively wound around the teeth. Turns of the phase coils are different from each other.
摘要翻译：电流检测单元通过将来自直流电源的直流电叠加到来自交流电源的交流电并通过电刷提供给直流电动机而引起的电流。提取单元提取检测到的电流的交流分量。角度检测单元根据所提取的交流分量检测电动机的旋转角度。方向检测单元根据所提取的交流分量的变化模式来检测电动机的旋转方向。电动机的铁芯在相邻的两个齿之间具有槽。这些槽分别容纳分别缠绕在齿上的相位线圈。相位线圈的转数彼此不同。

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