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1. 发明授权

US4816513A Automated polypeptide synthesis process 失效
标题翻译：自动多肽合成过程
公开(公告)号：US4816513A
公开(公告)日：1989-03-28
申请号：US53324
申请日：1987-05-22
申请人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
发明人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
IPC分类号： C07K1/00 , B01J19/00 , C07K1/04 , C40B40/10 , C40B60/14 , C08L89/00 , C07C103/52
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/00484 , B01J2219/00488 , B01J2219/0049 , B01J2219/00495 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another. The activator system also includes a temperature controlled concentrator vessel in which an activator solvent is replaced by a coupling solvent to enhance the coupling of the activated species to the peptide chain in the reaction vessel. Also included in the synthesizer system is a vortexer for affecting total washing of materials in the reaction vessel and the reaction vessel itself, an automated peptide resin sampling system, and an autodelivery system for providing individual containers of amino acid to the synthesizer in the order desired in the peptide sequence. A liquid sensor system is also included to monitor transitions between gases and liquids in specific tubes in the synthesizer in order to provide input signals to the computer system for control purposes. The computer system software which controls the operation of the synthesizer is organized according to a series of menus which allows the user of the system to select individual cycles of operation for each vessel in the synthesizer. In addition, an algorithm has been developed which provides for optimum efficiency in the production of a peptide for any given selection of cycles.
摘要翻译：提供了一种用于仅使用单个耦合自动构建高纯度多达50个氨基酸长度的多肽的装置。该装置包括用于一次接收受保护的氨基酸的激活系统，其具有在其中激活每个氨基酸的共同血管（活化剂容器）。还包括用于含有固相肽合成中用于连接肽链的树脂的反应容器。还提供了在计算机控制下操作的转移系统，以将活化物质从活化系统转移到反应容器中，并将氨基酸，试剂，气体和溶剂从设备的一部分转移到另一部分。活化剂体系还包括温度控制的浓缩容器，其中活化剂溶剂被偶联溶剂代替以增强活化物质与反应容器中的肽链的偶联。还包括在合成器系统中的是用于影响反应容器和反应容器本身中的材料的全部洗涤的涡流器，自动化肽树脂取样系统和用于按合适的顺序向合成器提供单个氨基酸容器的自动输送系统在肽序列中。还包括液体传感器系统以监测合成器中特定管中的气体和液体之间的转换，以便为控制目的向计算机系统提供输入信号。控制合成器操作的计算机系统软件根据一系列菜单进行组织，这些菜单允许系统的用户为合成器中的每个容器选择各个操作周期。此外，已经开发了一种算法，其提供用于任何给定选择周期的肽的生产的最佳效率。

2. 发明授权

US4668476A Automated polypeptide synthesis apparatus 失效
标题翻译：自动多肽合成仪
公开(公告)号：US4668476A
公开(公告)日：1987-05-26
申请号：US592638
申请日：1984-03-23
申请人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
发明人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
IPC分类号： C07K1/00 , B01J19/00 , C07K1/04 , C40B40/10 , C40B60/14 , C07K1/08
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/00484 , B01J2219/00488 , B01J2219/0049 , B01J2219/00495 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another.

3. 发明授权

US5186898A Automated polypeptide synthesis apparatus 失效
标题翻译：自动多肽合成仪
公开(公告)号：US5186898A
公开(公告)日：1993-02-16
申请号：US328488
申请日：1989-03-24
申请人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
发明人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marriott , Paul O. Ramstad , Eric S. Nordman
IPC分类号： B01J19/00 , C07K1/04 , C40B40/10 , C40B60/14
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/00484 , B01J2219/00488 , B01J2219/0049 , B01J2219/00495 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14 , Y10S436/81
摘要： An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another. The activator system also includes a temperature controlled concentrator vessel in which an activator solvent is replaced by a coupling solvent to enhance the coupling of the activated species to the peptide chain in the reaction vessel. Also included in the synthesizer system is a vortexer for affecting total washing of materials in the reaction vessel and the reaction vessel itself, an automated peptide resin sampling system, and an autodelivery system for providing individual containers of amino acid to the synthesizer in the order desired in the peptide sequence. A liquid sensor system is also included to monitor transitions between gases and liquids in specific tubes in the synthesizer in order to provide input signals to the computer system for control purposes. The computer system software which controls the operation of the synthesizer is organized according to a series of menus which allows the user of the system to select individual cycles of operation for each vessel in the synthesizer. In addition, an algorithm has been developed which provides for optimum efficiency in tbe production of a peptide for any given selection of cycles.
摘要翻译：提供了一种用于仅使用单个耦合自动构建高纯度多达50个氨基酸长度的多肽的装置。该装置包括用于一次接收受保护的氨基酸的激活系统，其具有在其中激活每个氨基酸的共同血管（活化剂容器）。还包括用于含有固相肽合成中用于连接肽链的树脂的反应容器。还提供了在计算机控制下操作的转移系统，以将活化物质从活化系统转移到反应容器中，并将氨基酸，试剂，气体和溶剂从设备的一部分转移到另一部分。活化剂体系还包括温度控制的浓缩容器，其中活化剂溶剂被偶联溶剂代替以增强活化物质与反应容器中的肽链的偶联。还包括在合成器系统中的是用于影响反应容器和反应容器本身中的材料的全部洗涤的涡流器，自动化肽树脂取样系统和用于按合适的顺序向合成器提供单个氨基酸容器的自动输送系统在肽序列中。还包括液体传感器系统以监测合成器中特定管中的气体和液体之间的转换，以便为控制目的向计算机系统提供输入信号。控制合成器操作的计算机系统软件根据一系列菜单进行组织，这些菜单允许系统的用户为合成器中的每个容器选择各个操作周期。此外，已经开发了一种算法，其为任何给定的循环选择提供肽的生产的最佳效率。

4. 发明授权

US5273715A Automated system for providing a sequence of chemicals to a reaction process 失效
标题翻译：用于向反应过程提供一系列化学物质的自动化系统
公开(公告)号：US5273715A
公开(公告)日：1993-12-28
申请号：US973137
申请日：1992-11-06
申请人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marplott , Paul O. Ramstad
发明人： John Bridgham , Timothy G. Geiser , Michael W. Hunkapiller , Stephen B. H. Kent , Mark P. Marplott , Paul O. Ramstad
IPC分类号： B01J19/00 , C07K1/04 , C40B40/10 , C40B60/14 , G01N21/00 , B01L3/02 , B01L11/00 , G01N31/00
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00389 , B01J2219/00484 , B01J2219/00488 , B01J2219/0049 , B01J2219/00495 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： An automated system for providing a preselected sequence of chemicals to a reaction. This apparatus includes a track on which a set of cartridges are placed in a preselected order corresponding to an order in which they are to be used in a reaction process. These cartridges are moved past a point at which these chemicals are extracted for use in the process. The chemicals are preferably in liquid form and are contained in containing having a top seal through which a needle can penetrate to extract chemicals for use in the process. These containers preferably contain the aliquot portion needed for the process, thereby providing a mechanism for providing accurate amounts of each chemical to the process.
摘要翻译：一种用于向反应提供预选序列化学物质的自动化系统。该装置包括一个轨道，在该轨道上以预选的顺序放置一组墨盒，该预定顺序与它们在反应过程中使用的顺序相对应。这些墨盒被移动通过这些化学品提取的点，以用于该过程。化学品优选为液体形式，并且包含含有顶部密封件，针可穿过该顶部密封件以提取用于该方法中的化学品。这些容器优选地含有该方法所需的等分试样部分，从而提供了一种用于向该方法提供精确量的每种化学品的机理。

5. 发明申请

US20120138462A1 ELECTROWETTING DISPENSING DEVICES AND RELATED METHODS 有权
标题翻译：电镀分配装置及相关方法
公开(公告)号：US20120138462A1
公开(公告)日：2012-06-07
申请号：US13311269
申请日：2011-12-05
申请人： Charles S. Vann , Debjyoti Banerjee , Timothy G. Geiser , James C. Nurse , Nigel P. Beard
发明人： Charles S. Vann , Debjyoti Banerjee , Timothy G. Geiser , James C. Nurse , Nigel P. Beard
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44743 , B01J4/008 , B01J2219/00315 , B01J2219/0036 , B01J2219/00418 , B01J2219/00653 , B01L3/0241 , B01L3/0268 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/02 , B01L2400/0409 , B01L2400/0427
摘要： A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.
摘要翻译：用于分配用于生物分析的液体的方法可以包括通过电润湿定位待分配的液体。定位可以包括使液体与多个预定位置对准。该方法还可以包括通过分别与预定位置对准的多个开口分配来自多个预定位置的对准的液体。分配可以经由电润湿。

6. 发明申请

US20100236930A1 Electrowetting Dispensing Devices and Related Methods 有权
标题翻译：电喷分配装置及相关方法
公开(公告)号：US20100236930A1
公开(公告)日：2010-09-23
申请号：US12709402
申请日：2010-02-19
申请人： Charles S. Vann , Debjyoti Banerjee , Timothy G. Geiser , James C. Nurse , Nigel P. Beard
发明人： Charles S. Vann , Debjyoti Banerjee , Timothy G. Geiser , James C. Nurse , Nigel P. Beard
IPC分类号： G01N27/26
CPC分类号： G01N27/44743 , B01J4/008 , B01J2219/00315 , B01J2219/0036 , B01J2219/00418 , B01J2219/00653 , B01L3/0241 , B01L3/0268 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/02 , B01L2400/0409 , B01L2400/0427
摘要： A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.
摘要翻译：用于分配用于生物分析的液体的方法可以包括通过电润湿定位待分配的液体。定位可以包括使液体与多个预定位置对准。该方法还可以包括通过分别与预定位置对准的多个开口分配来自多个预定位置的对准的液体。分配可以经由电润湿。

7. 发明申请

US20110318740A1 Reduced Interference from Single Strand Binding Proteins 审中-公开
标题翻译：降低单链结合蛋白的干扰
公开(公告)号：US20110318740A1
公开(公告)日：2011-12-29
申请号：US13149697
申请日：2011-05-31
申请人： Robert G. Eason , Timothy G. Geiser , Vladimir I. Bashkirov , Kristian M. Scaboo
发明人： Robert G. Eason , Timothy G. Geiser , Vladimir I. Bashkirov , Kristian M. Scaboo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6846 , C12Q2522/101 , C12Q2561/109 , C12Q2565/519 , C12Q1/686
摘要： Disclosed, for example, are methods for detecting at least one target polynucleotide comprising cleaving a flap from a polynucleotide probe that is hybridized to a complementary target polynucleotide, and hybridizing the cleaved flap to a complementary capture probe immobilized on a surface, wherein said cleaving and/or hybridizing occurs in the presence of a single strand binding protein that is capable of binding single-stranded DNA, but that can bind neither the flap nor the capture probe. The cleaved flap and the complementary capture probe may each comprise PNA, RNA, LNA, L-DNA or other modified nucleotides or chimeras thereof that do not significantly bind to the single strand binding protein.
摘要翻译：例如，公开了用于检测至少一种靶多核苷酸的方法，所述至少一种靶多核苷酸包括从互补靶多核苷酸杂交的多核苷酸探针切割瓣，并将切割的瓣与固定在表面上的互补捕获探针杂交，其中所述切割和 /或杂交发生在能够结合单链DNA的单链结合蛋白存在下，但是既不结合皮瓣也不能结合捕获探针。切割的瓣和互补捕获探针可以各自包含不显着结合单链结合蛋白的PNA，RNA，LNA，L-DNA或其它修饰的核苷酸或嵌合体。

8. 发明申请

US20080227124A1 Solid phases optimized for chemiluminescent detection 审中-公开
公开(公告)号：US20080227124A1
公开(公告)日：2008-09-18
申请号：US12076235
申请日：2008-03-14
申请人： Brooks Edwards , Timothy G. Geiser , Steven M. Menchen , Alison L. Sparks , John C. Voyta
发明人： Brooks Edwards , Timothy G. Geiser , Steven M. Menchen , Alison L. Sparks , John C. Voyta
IPC分类号： G01N33/53 , G01N21/76 , B05D3/02 , C12Q1/00
CPC分类号： C12Q1/6823 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B50/14 , G01N33/54393 , Y10S436/805 , Y10S436/823
摘要： Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.

9. 发明申请

US20080213755A1 Method and Device for Detection of Nucleic Acid Sequences 审中-公开
标题翻译：用于检测核酸序列的方法和装置
公开(公告)号：US20080213755A1
公开(公告)日：2008-09-04
申请号：US10585382
申请日：2005-01-12
申请人： Timothy G. Geiser , H. Pin Kao
发明人： Timothy G. Geiser , H. Pin Kao
IPC分类号： C12Q1/68 , B01J19/00 , G01N21/64 , G01N33/00 , G01N33/68
CPC分类号： B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0684 , B01L2200/10 , B01L2300/0627 , B01L2300/0816 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , C12Q2565/629 , Y10T436/143333
摘要： A device (420) and method for real-time amplification and detection of a large number of target nucleic acids contained in a large number of biological samples is provided. The device (420) can comprise a substrate (422) and an optically transparent cover, wherein the substrate can comprise a first surface, at least one sample receiving chamber (430), a distributor channel (434), at least one reaction chamber (428), and a vent (440) for each reaction chamber. The substrate can comprise a material having a thermal conductivity of at least about 0.25 W/m° K.
摘要翻译：提供了一种用于实时扩增和检测包含在大量生物样品中的大量靶核酸的装置（420）和方法。所述装置（420）可包括基底（422）和光学透明盖，其中所述基底可包括第一表面，至少一个样品接收室（430），分配器通道（434），至少一个反应室 428）和每个反应室的排气口（440）。衬底可以包括具有至少约0.25W / m°K的热导率的材料。

10. 发明授权

US07368296B2 Solid phases optimized for chemiluminescent detection 有权
标题翻译：为化学发光检测优化的固相
公开(公告)号：US07368296B2
公开(公告)日：2008-05-06
申请号：US10046730
申请日：2002-01-17
申请人： Brooks Edwards , Timothy G. Geiser , Steven M. Menchen , Alison L. Sparks , John C. Voyta
发明人： Brooks Edwards , Timothy G. Geiser , Steven M. Menchen , Alison L. Sparks , John C. Voyta
IPC分类号： G01N33/545 , G01N33/533 , C07C211/62
CPC分类号： C12Q1/6823 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B50/14 , G01N33/54393 , Y10S436/805 , Y10S436/823
摘要： Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.
摘要翻译：提供了化学发光测定的固体支持物。固体支持物包括共价或物理连接到支持物表面上的多个探针和结合到表面或载体的大部分中的化学发光增强部分。固体支持物可以是包含邻近阳离子微凝胶层的上探针结合层（例如，吖内酯聚合物层或多孔功能性聚酰胺层）的多层载体。吖内酯官能聚合物可以是与乙二胺交联的二甲基丙烯酰胺和乙烯基内酯的共聚物。阳离子微凝胶层可以是交联的含季铵盐的聚合物。还提供了使用固体支持物进行化学发光测定的方法和试剂盒。试剂盒包括二氧杂环丁烷底物，生物聚合物探针 - 酶复合物和固体支持物。固体支持物可以是与阳离子微凝胶层相邻的吖内酯官能聚合物层; 与阳离子微凝胶层相邻的多孔聚酰胺官能层; 或季铵化的吖内酯官能聚合物层。

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