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1. 发明申请

US20070123697A1 Process for purifying interferon beta 有权
标题翻译：干扰素β的纯化方法
公开(公告)号：US20070123697A1
公开(公告)日：2007-05-31
申请号：US10581597
申请日：2004-12-04
申请人： Ji Sook Park , Jong Chung , Min Baek , Jee Ahn , Ki Kim , Hyung Park , Dong Lee , Myung Oh
发明人： Ji Sook Park , Jong Chung , Min Baek , Jee Ahn , Ki Kim , Hyung Park , Dong Lee , Myung Oh
IPC分类号： C07K14/56
CPC分类号： C07K14/565 , C12P21/02
摘要： Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and cation exchange chromatography, wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt % of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt % of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt % of propylene glycol and 1-2M NaCl.
摘要翻译：提供了一种从重组人类干扰素β的培养物中纯化人类干扰素β的方法，包括进行亲和层析和阳离子交换层析，其中亲和层析包括：将含干扰素β的培养物吸附到平衡的亲和色谱柱上，然后洗涤用平衡缓冲溶液; 用含有30-60重量％丙二醇的pH 6.5-7.5的洗涤缓冲溶液A和pH6.5-7.5的洗涤缓冲液B洗涤柱子，其中含有10-30重量％的丙二醇和1-2M的NaCl; 并用含有40-60重量％丙二醇和1-2M NaCl的pH 6.5-7.5的缓冲溶液洗脱人类含有干扰素β的级分。

2. 发明申请

US20070093649A1 Process for purifying interferon beta 有权
标题翻译：干扰素β的纯化方法
公开(公告)号：US20070093649A1
公开(公告)日：2007-04-26
申请号：US10581602
申请日：2004-12-04
申请人： Ji Sook Park , Min Baek , Jee Ahn , Ki Kim , Hyung Park , Dong Lee , Myung Oh
发明人： Ji Sook Park , Min Baek , Jee Ahn , Ki Kim , Hyung Park , Dong Lee , Myung Oh
IPC分类号： C07K14/56
CPC分类号： C07K14/565 , C07K5/06139
摘要： Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt % of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt % of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt % of propylene glycol and 1-2M NaCl.
摘要翻译：提供了一种从重组人类干扰素β培养物中纯化人类干扰素β的方法，包括进行亲和层析和反相高效液相色谱（RP-HPLC），其中亲和层析包括：吸附含有干扰素β的培养物平衡的亲和层析柱，然后用平衡缓冲液洗涤; 用含有30-60重量％丙二醇的pH 6.5-7.5的洗涤缓冲溶液A和pH6.5-7.5的洗涤缓冲液B洗涤柱子，其中含有10-30重量％的丙二醇和1-2M的NaCl; 并用含有40-60重量％丙二醇和1-2M NaCl的pH 6.5-7.5的缓冲溶液洗脱人类含有干扰素β的级分。

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