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1. 发明申请

US20050281792A1 Phytases, nucleic acids encoding them and methods of making and using them 有权
标题翻译：植酸酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US20050281792A1
公开(公告)日：2005-12-22
申请号：US10933115
申请日：2004-09-01
申请人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , William Baum , Dan Robertson , Paul Zorner
发明人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , William Baum , Dan Robertson , Paul Zorner
IPC分类号： A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/211 , A61K38/00 , C12N1/21 , C12N9/16 , A61K45/00 , A61K38/46
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译：一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。此外，该酶在低pH下具有改善的蛋白酶稳定性。修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。

2. 发明申请

US20050246780A1 Phytases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：植酸酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US20050246780A1
公开(公告)日：2005-11-03
申请号：US11056354
申请日：2005-02-11
申请人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , Eric Mathur
发明人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , Eric Mathur
IPC分类号： A01H1/00 , A01K67/00 , A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/211 , A61K38/00 , C07H21/04 , C12N1/21 , C12N5/04 , C12N9/16 , C12N15/82
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译：本发明提供了分离和重组的植酸酶。一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。该酶可以从重组宿主细胞产生。本发明的植酸酶可以用于有助于在需要时消化植酸盐。特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。本发明的植酸酶可以是耐热和/或耐热的。还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。

3. 发明申请

US20050208121A1 Polymeric cannulae proteins, nucleic acids encoding them and methods for making and using them 审中-公开
标题翻译：聚合插管蛋白，编码它们的核酸以及制备和使用它们的方法
公开(公告)号：US20050208121A1
公开(公告)日：2005-09-22
申请号：US11046203
申请日：2005-01-28
申请人： Nelson Barton , Eileen O'Donoghue , Gerhard Frey , Jay Short , W. Lafferty , Kevin Chow
发明人： Nelson Barton , Eileen O'Donoghue , Gerhard Frey , Jay Short , W. Lafferty , Kevin Chow
IPC分类号： A01K67/00 , A61K33/44 , A61K38/17 , C07B57/00 , C07H21/04 , C07K14/195 , C07K14/705 , C12P21/04 , G01N33/573 , G01N33/68
CPC分类号： B82Y5/00 , A01K2217/05 , A61K9/127 , A61K9/1274 , B82Y30/00 , C07K14/195 , C07K2319/00 , G01N33/573 , G01N33/68 , G01N2333/195
摘要： The invention provides chimeric cannulae polypeptides and methods for making and using them. In one aspect, the invention provides compositions and methods for the identification, separation or synthesis of proteins or ligands. In one aspect, the invention provides compositions and methods for making and using nanotubules. In one aspect, the invention provides compositions and methods for the selection and purification of chiral compositions from racemic mixtures. The invention provides compositions comprising polymers prepared by self-assembly of a plurality of monomeric polypeptide units, including nanorobots, biochips, drug delivery systems. In one aspect, the polymer can form a nanotube, and the polymer (nanotubule) can encapsulate a drug molecule—a drug delivery systems of the invention. A drug delivery system of the invention may be delivered to a particular location of human body to effectively cure a disease or treat a symptom. In one aspect, a targeting vector can be attached to a monomeric polypeptide unit or a polymer to facilitate the targeting of the drug delivery system of the invention.
摘要翻译：本发明提供了嵌合插管多肽及其制备和使用方法。一方面，本发明提供用于鉴定，分离或合成蛋白质或配体的组合物和方法。一方面，本发明提供了制备和使用纳米管的组合物和方法。一方面，本发明提供了用于从外消旋混合物中选择和纯化手性组合物的组合物和方法。本发明提供了包含通过自组装多个单体多肽单元制备的聚合物的组合物，包括纳米机器人，生物芯片，药物递送系统。在一个方面，聚合物可以形成纳米管，并且聚合物（纳米管）可以包封药物分子 - 本发明的药物递送系统。本发明的药物递送系统可以被递送到人体的特定位置以有效地治愈疾病或治疗症状。在一个方面，靶向载体可以连接到单体多肽单元或聚合物以促进本发明的药物递送系统的靶向。

4. 发明申请

US20060275804A1 Enzymes having dehalogenase activity and methods of use thereof 有权
标题翻译：具有脱卤素酶活性的酶及其使用方法
公开(公告)号：US20060275804A1
公开(公告)日：2006-12-07
申请号：US11418828
申请日：2006-05-05
申请人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
发明人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C12P7/40 , C12N9/02 , C12N1/21 , C12N1/18 , C12N5/04 , C12N5/06
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。另外还提供了设计新的脱卤素酶的方法及其使用方法。脱盐酶在增加的pH和温度下具有增加的活性和稳定性。

5. 发明申请

US20060035216A1 Enzymes having dehalogenase activity and methods of use thereof 有权
标题翻译：具有脱卤素酶活性的酶及其使用方法
公开(公告)号：US20060035216A1
公开(公告)日：2006-02-16
申请号：US10000997
申请日：2001-11-30
申请人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
发明人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
IPC分类号： C12Q1/68 , C07H21/04 , G06F19/00 , G01N33/48 , G01N33/50
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding alkane dehalogenases. In addition methods of designing new dehalogenases and of use thereof are also provided. The dehalogenases have increased activity and at increased pH and temperature.
摘要翻译：本发明涉及卤烷烃脱卤酶和编码烷烃脱卤酶的多核苷酸。此外，还提供了设计新的脱卤素酶及其用途的方法。脱卤酶具有增加的活性和增加的pH和温度。

6. 发明授权

US07459172B2 Method of making a protein polymer and uses of the polymer 失效
标题翻译：制备蛋白质聚合物的方法和聚合物的用途
公开(公告)号：US07459172B2
公开(公告)日：2008-12-02
申请号：US09997807
申请日：2001-11-30
申请人： Jay Short , Eric J. Mathur , W. Michael Lafferty , Nelson Barton , Kevin Chow
发明人： Jay Short , Eric J. Mathur , W. Michael Lafferty , Nelson Barton , Kevin Chow
IPC分类号： A61K31/74 , A61K38/16
CPC分类号： B82Y30/00 , A61K9/0092 , A61K9/1274 , C07K14/195
摘要： A polymer is prepared by self-assembly of a plurality of monomeric polypeptide units. The polymer tends to form a nanotube and is capable of encapsulating a particular drug molecule. Once encapsulated in the polymer of the present invention, the drug molecule may be delivered to a particular location of human body to effectively cure a disease or treat a symptom.Generally, the monomeric polypeptide unit of the present invention has a sequence found in Pyrodictium abyssi, a microorganism that produces an extracellular network having hollow protein tubes, or a sequence substantially identical thereto. The monomeric polypeptide may be mass produced using recombinant biotechnologies and be polymerized into the polymer of the present invention. One or more additional targeting vector may be attached to the monomeric polypeptide unit or the polymer to facilitate the targeting of the drug molecule that may be held there within. The sequence contained in the monomeric polypeptide unit may be further optimized using one or more technique selected from Gene Site Saturation Mutagenesis and GeneReasembly™.
摘要翻译：通过多个单体多肽单元的自组装制备聚合物。聚合物倾向于形成纳米管并且能够封装特定的药物分子。一旦包封在本发明的聚合物中，药物分子可以被递送到人体的特定位置以有效治愈疾病或治疗症状。通常，本发明的单体多肽单元具有在黑腹果蝇（Pyrodictium abyssi）中发现的序列，该菌株产生具有中空蛋白管的细胞外网络或与其基本相同的序列。单体多肽可以使用重组生物技术大规模生产并聚合成本发明的聚合物。一个或多个另外的靶向载体可以连接到单体多肽单元或聚合物，以促进可能在其中保持的药物分子的靶向。可以使用选自Gene Site Saturation Mutagenesis和GeneReasembly TM的一种或多种技术进一步优化单体多肽单元中所含的序列。

7. 发明申请

US20100172968A1 CHIMERIC CANNULAE PROTEINS AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译： CHIMERIC CANNULAE蛋白质及其制备和使用方法
公开(公告)号：US20100172968A1
公开(公告)日：2010-07-08
申请号：US11929781
申请日：2007-10-30
申请人： Jay Short , Eric J. Mathur , W. Michael Lafferty , Nelson Barton , Kevin Chow
发明人： Jay Short , Eric J. Mathur , W. Michael Lafferty , Nelson Barton , Kevin Chow
IPC分类号： A61K9/48 , A61K9/10 , C12N15/63 , C07K14/00 , C07K16/00
CPC分类号： B82Y30/00 , A61K9/0092 , A61K9/1274 , C07K14/195
摘要： A polymer is prepared by self-assembly of a plurality of monomeric polypeptide units. The polymer tends to form a nanotube and is capable of encapsulating a particular drug molecule. Once encapsulated in the polymer of the present invention, the drug molecule may be delivered to a particular location of human body to effectively cure a disease or treat a symptom.Generally, the monomeric polypeptide unit of the present invention has a sequence found in Pyrodictium abyssi, a microorganism that produces an extracellular network having hollow protein tubes, or a sequence substantially identical thereto. The monomeric polypeptide may be mass produced using recombinant biotechnologies and be polymerized into the polymer of the present invention. One or more additional targeting vector may be attached to the monomeric polypeptide unit or the polymer to facilitate the targeting of the drug molecule that may be held there within. The sequence contained in the monomeric polypeptide unit may be further optimized using one or more technique selected from Gene Site Saturation Mutagenesis and GeneReasembly.
摘要翻译：通过多个单体多肽单元的自组装制备聚合物。聚合物倾向于形成纳米管并且能够封装特定的药物分子。一旦包封在本发明的聚合物中，药物分子可以被递送到人体的特定位置以有效治愈疾病或治疗症状。通常，本发明的单体多肽单元具有在黑腹果蝇（Pyrodictium abyssi）中发现的序列，该菌株产生具有中空蛋白管的细胞外网络或与其基本相同的序列。单体多肽可以使用重组生物技术大规模生产并聚合成本发明的聚合物。一个或多个另外的靶向载体可以连接到单体多肽单元或聚合物，以促进可能在其中保持的药物分子的靶向。可以使用选自基因位点饱和诱变和基因重组的一种或多种技术进一步优化单体多肽单元中所含的序列。

8. 发明申请

US20100273659A1 Nitrilases, Nucleic Acids Encoding Them and Methods for Making and Using Them 有权
公开(公告)号：US20100273659A1
公开(公告)日：2010-10-28
申请号：US12529340
申请日：2008-02-29
申请人： Grace DeSantis , Jennifer Chaplin , Ellen Chi , Aileen Milan , Jay Short , David Weiner , Mark Madden , Darcy Madden , Dan Robertson
发明人： Grace DeSantis , Jennifer Chaplin , Ellen Chi , Aileen Milan , Jay Short , David Weiner , Mark Madden , Darcy Madden , Dan Robertson
IPC分类号： C40B20/00 , C12N15/55 , C12N15/63 , C12N9/78 , C12P7/40 , C12P7/42 , C12P13/00 , C12P7/54 , C12P7/56 , C12P19/34 , C12Q1/68 , C12P1/00 , C12Q1/34
CPC分类号： C12P7/42 , C12N9/78 , C12P13/002 , C12P13/004 , C12P13/005 , C12P13/04 , C12P41/007
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

9. 发明授权

US08334125B2 Nucleic acids encoding nitrilases 有权
标题翻译：编码腈水解酶的核酸
公开(公告)号：US08334125B2
公开(公告)日：2012-12-18
申请号：US13169853
申请日：2011-06-27
申请人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Darcy Madden, legal representative , Mark Burk , Dan Robertson
发明人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Mark Burk , Dan Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , C12Q1/68 , A61K38/00 , C07H21/04 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及腈水解酶和编码腈水解酶的核酸。另外还提供了设计新腈水解酶的方法及其使用方法。在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

10. 发明授权

US07993901B2 Nitrilases and methods for making and using them 有权
标题翻译：腈水解酶及其制备和使用方法
公开(公告)号：US07993901B2
公开(公告)日：2011-08-09
申请号：US12629818
申请日：2009-12-02
申请人： Grace Desantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Marcy Madden, legal representative , Mark Burk , Dan Robertson
发明人： Grace Desantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Mark Burk , Dan Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , C12Q1/68 , A61K38/00 , C07H21/04 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及腈水解酶和编码腈水解酶的核酸。另外还提供了设计新腈水解酶的方法及其使用方法。在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

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