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1. 发明申请

WO2011002824A1 PRETREATMENT OF BIOMASS 审中-公开
标题翻译：生物量的预处理
公开(公告)号：WO2011002824A1
公开(公告)日：2011-01-06
申请号：PCT/US2010/040502
申请日：2010-06-29
申请人： QTEROS, INC. , LATOUF, William, G. , GRAY, Kevin, Gray
发明人： LATOUF, William, G. , GRAY, Kevin, Gray
IPC分类号： C12N1/00 , C12N1/20
CPC分类号： C12P7/10 , C12P7/065 , C12P7/16 , C12P2201/00 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： Compositions and methods for producing alcohols and other fermentation end-products by fermenting alkaline or acid pretreated plant material feedstocks with a microorganism are disclosed herein.
摘要翻译：本文公开了通过用微生物发酵碱性或酸预处理的植物材料原料来生产醇和其它发酵终产物的组合物和方法。

2. 发明授权

US08551754B2 Amylases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：淀粉酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US08551754B2
公开(公告)日：2013-10-08
申请号：US13283119
申请日：2011-10-27
申请人： Kevin Gray , Nahla Aboushadi , James Garrett
发明人： Kevin Gray , Nahla Aboushadi , James Garrett
IPC分类号： C12N9/28 , C12N9/26 , C12P19/14 , C12P7/02 , C12P7/06 , D06M16/00 , C12S3/00 , C12C11/00 , A21D2/00 , A23L1/00 , C07H21/04 , C07K1/00
CPC分类号： C12N9/2411 , A21D8/042 , A23K10/14 , A23K20/189 , A23L29/06 , A23L29/35 , A23V2002/00 , C11D3/386 , C12C5/004 , C12N9/2417 , C12N9/2428 , C12N9/2451 , C12Y302/01 , C12Y302/01001 , C12Y302/01002 , Y02E50/17
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.
摘要翻译：一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。本发明的多肽可用作淀粉酶以催化淀粉水解成糖。

3. 发明申请

US20110016545A1 Xylanases, Nucleic Acids Encoding Them and Methods For Making and Using Them 有权
标题翻译：木聚糖酶，编码它们的核酸及其制备和使用方法
公开(公告)号：US20110016545A1
公开(公告)日：2011-01-20
申请号：US12681604
申请日：2008-08-01
申请人： Kevin Gray , Reinhard Dirmeier
发明人： Kevin Gray , Reinhard Dirmeier
IPC分类号： A01K67/027 , C07H21/04 , C12N15/63 , C12N5/10 , C12N1/15 , C12N1/19 , A01H5/00 , C12N9/24 , C12N11/00 , C07K16/40 , C12P19/02 , D21C3/00 , D21C5/02 , D21H17/22
CPC分类号： C12P19/14 , C12N9/248 , C12P19/02 , D21H17/22 , Y02E50/16 , Y02T50/678 , Y10T428/249921
摘要： The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及具有木聚糖酶，甘露聚糖酶和/或葡聚糖酶活性的酶，例如催化内部-1,4-木糖苷键或内切-1,4-葡聚糖酶键的水解; 和/或将线性多糖β-1,4-木聚糖降解成木糖。因此，本发明提供了分解作为植物细胞壁的主要成分的半纤维素的方法和方法，包括在任何植物或木材或木制品，木材废料，纸浆，纸制品中水解半纤维素的方法和方法，废纸或副产品。此外，还提供了设计新的木聚糖酶，甘露聚糖酶和/或葡聚糖酶的方法及其使用方法。木聚糖酶，甘露聚糖酶和/或葡聚糖酶在增加的pH和温度下具有增加的活性和稳定性。

4. 发明申请

US20090300365A1 Vehicle Diagnostic System Security with Memory Card 审中-公开
标题翻译：带有存储卡的车辆诊断系统安全
公开(公告)号：US20090300365A1
公开(公告)日：2009-12-03
申请号：US12129992
申请日：2008-05-30
申请人： Robert Karmes , Kevin Gray , Scott Morris
发明人： Robert Karmes , Kevin Gray , Scott Morris
IPC分类号： H04L9/12
CPC分类号： G06F21/125 , G06F21/572
摘要： A method and system are provided to authenticate a software stored on a computing device such as vehicle diagnostic tool. The system generates and stores encrypted information such as a memory media and the media access control address of the vehicle diagnostic tool. The encrypted information can be sent to an authentication server which returns encrypted authentication information that is used to validate the software for a period of time.
摘要翻译：提供了一种方法和系统来验证存储在诸如车辆诊断工具的计算设备上的软件。系统生成并存储诸如存储介质和车辆诊断工具的媒体访问控制地址之类的加密信息。可以将加密信息发送到认证服务器，该认证服务器返回用于在一段时间内验证软件的加密认证信息。

5. 发明申请

US20070161099A1 METHODS FOR USING POLYPEPTIDES HAVING AMYLASE ACTIVITY AND NUCLEIC ACIDS ENCODING THEM 有权
标题翻译：使用具有AMYLASE活性的多肽和编码其的核酸的方法
公开(公告)号：US20070161099A1
公开(公告)日：2007-07-12
申请号：US11621543
申请日：2007-01-09
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
IPC分类号： C12P7/06 , C12N9/32 , C12N5/10 , C12N5/06 , C12N1/21
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The invention relates to uses of polypeptides having amylase activity, nucleic acids encoding them, and methods for making and using them. For example, in one aspect, the invention provides methods for making alcohol using polypeptides of the invention. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, for hydrolyzing a starch, comprising use of polypeptides of the invention, or polypeptides encoded by the nucleic acids of this invention.
摘要翻译：本发明涉及具有淀粉酶活性的多肽的用途，编码它们的核酸以及制备和使用它们的方法。例如，一方面，本发明提供了使用本发明多肽制备醇的方法。一方面，具有淀粉酶活性的多肽在酸性，中性和碱性pH和升高的温度下具有增加的活性和稳定性。一方面，本发明提供用于生产高麦芽糖或高葡萄糖浆或混合糖浆的醇的方法，用于液化包含淀粉的组合物，用于水解淀粉，包括使用本发明的多肽，或由本发明的核酸编码的多肽。

6. 发明申请

US20070157329A1 Amylases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：淀粉酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US20070157329A1
公开(公告)日：2007-07-05
申请号：US10547957
申请日：2004-03-08
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Kevin Gray , Janne Kerovuo , Malgorzata Slupska , Nelson Barton , Eileen O'Donoghue , Carl Miller
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Kevin Gray , Janne Kerovuo , Malgorzata Slupska , Nelson Barton , Eileen O'Donoghue , Carl Miller
IPC分类号： A01K67/027 , C12Q1/68 , C07H21/04 , C12P21/06 , A61K38/47 , C12P19/02 , C12N9/32 , A21D8/02 , C12C11/00 , C12P7/06 , C12N15/82 , C12N5/06
CPC分类号： A21D8/042 , A61K38/00 , C12C5/004 , C12N9/2411 , C12N9/2414 , C12N9/2428 , C12P19/14 , C12P19/16 , C12P19/20 , Y02E50/17
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for malting and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating.
摘要翻译：一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及用于发芽和使用这些多核苷酸和多肽的方法。在一个方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化淀粉水解成糖。一方面，本发明提供包含通过胶乳聚合物涂层涂覆的所需成分的延迟释放组合物。

7. 发明申请

US20060275804A1 Enzymes having dehalogenase activity and methods of use thereof 有权
标题翻译：具有脱卤素酶活性的酶及其使用方法
公开(公告)号：US20060275804A1
公开(公告)日：2006-12-07
申请号：US11418828
申请日：2006-05-05
申请人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
发明人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C12P7/40 , C12N9/02 , C12N1/21 , C12N1/18 , C12N5/04 , C12N5/06
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。另外还提供了设计新的脱卤素酶的方法及其使用方法。脱盐酶在增加的pH和温度下具有增加的活性和稳定性。

8. 发明申请

US20050281792A1 Phytases, nucleic acids encoding them and methods of making and using them 有权
标题翻译：植酸酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US20050281792A1
公开(公告)日：2005-12-22
申请号：US10933115
申请日：2004-09-01
申请人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , William Baum , Dan Robertson , Paul Zorner
发明人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , William Baum , Dan Robertson , Paul Zorner
IPC分类号： A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/211 , A61K38/00 , C12N1/21 , C12N9/16 , A61K45/00 , A61K38/46
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译：一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。此外，该酶在低pH下具有改善的蛋白酶稳定性。修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。

9. 发明申请

US20050240351A1 Method for determining a stuck point for pipe, and free point logging tool 有权
标题翻译：用于确定管道粘滞点的方法和自由点测井工具
公开(公告)号：US20050240351A1
公开(公告)日：2005-10-27
申请号：US10967588
申请日：2004-10-18
申请人： Kevin Gray
发明人： Kevin Gray
IPC分类号： G01V9/00
CPC分类号： E21B47/0905 , E21B47/09 , E21B47/091
摘要： A method and apparatus for determining the location of stuck pipe are provided. In one embodiment, the method includes the step of attaching a free point logging tool to a working line such as a slickline or wireline. The free point logging tool has a freepoint sensor and, optionally, an acoustic sensor. The freepoint sensor acquires magnetic permeability data in a string of pipe, while the acoustic sensor acquires acoustic data in the pipe. Two sets of data for each sensor are acquired—one in which the pipe under investigation is unstressed, and one in which the pipe is stressed. The first set and second sets of magnetic permeability data are compared to determine the stuck point location for the pipe. The first and second sets of acoustic data are compared to determine the nature in which the pipe is stuck.
摘要翻译：提供了一种用于确定卡管的位置的方法和装置。在一个实施例中，该方法包括将自由点测井工具附接到诸如光滑线或有线线的工作线的步骤。自由点测井工具具有自由点传感器和可选的声学传感器。自由点传感器获取管道中的磁导率数据，而声学传感器获取管道中的声学数据。获取每个传感器的两组数据 - 一个是被调查的管子不受应力的管道，另一个是管道受压的数据。比较第一组和第二组导磁率数据，以确定管道的卡住位置。比较第一组和第二组声学数据以确定管被卡住的性质。

10. 发明申请

US20050211429A1 Dual sensor freepoint tool 有权
公开(公告)号：US20050211429A1
公开(公告)日：2005-09-29
申请号：US11052939
申请日：2005-02-08
申请人： Kevin Gray , James Estes
发明人： Kevin Gray , James Estes
IPC分类号： E21B29/00 , E21B31/00 , E21B47/09
CPC分类号： E21B29/005 , E21B31/002 , E21B47/09
摘要： An apparatus and method of determining the point at which a tubular is stuck within another tubular or a wellbore by applying a tensile or torsional force to the stuck tubular and measuring the response of various locations within the tubular. In addition, the apparatus may be combined with a cutting tool to separate the free portion of the tubular from the stuck portion.

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