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USH1498H Polygenic trait determinants: maize dwarf mosaic virus 失效
标题翻译：多基因性状决定因素：玉米矮花叶病毒
公开(公告)号：USH1498H
公开(公告)日：1995-11-07
申请号：US50965
申请日：1993-04-21
申请人： Michael G. Murray , Jane H. Cramer , Jeanne Romero-Severson , David West , Yu Ma
发明人： Michael G. Murray , Jane H. Cramer , Jeanne Romero-Severson , David West , Yu Ma
IPC分类号： A01H5/10 , A01H1/00
CPC分类号： A01H5/10
摘要： A set of nucleic acid probes useful for tracking Maize Dwarf Mosaic Virus Resistance (MDMV), a polygenic trait, is provided. Chromosome segments are identified enabling isolation of the genes governing this trait.A general method for identifying probes useful for tracking and introgressing polygenic traits into elite genomes and identifying chromosome segments governing the traits is also provided. This method involves the analysis of RFLP polymorphisms between parent donor and recipient genotypes and observed phenotypic data using multiple regression by leaps and bounds ("leaps"), followed by standard multiple regression applied to the "leaps" data. The "leaps" data may be used to identify flanking markers, and epistasis may be determined by analysis of the multiple regression data.Kits comprising a subset of the most useful probes (those most closely linked to the trait of interest) are provided. The kits may also comprise flanking probes. Flanking probes used in combination with the most closely linked probes are useful in identifying situations in which donor DNA tends to move in clumps and recovering rare individuals in which traits of interest have separated from surrounding donor DNA, so that elite recipient DNA may be maximized.
摘要翻译：提供了一套用于跟踪玉米矮花叶病毒抗性（MDMV）的多核基因特征的核酸探针。鉴定染色体片段能够分离控制该性状的基因。还提供了用于鉴定用于跟踪和将多基因性状渗入精英基因组并鉴定控制性状的染色体片段的探针的一般方法。该方法涉及使用多跳回归（“跨越”）分析父母供体和受体基因型之间的RFLP多态性，并使用多重回归（“跨越”）观察表型数据，然后应用于“跨越”数据的标准多元回归。 “跳跃”数据可以用于鉴定侧翼标记，并且可以通过分析多个回归数据来确定上位稳定性。提供了包含最有用的探针子集（最感兴趣的特征）的试剂盒。试剂盒还可以包含侧翼探针。与最紧密连接的探针结合使用的侧翼探针可用于鉴定供体DNA倾向于移动并且回收其中感兴趣的性状与周围供体DNA分离的稀有个体的情况，从而可以使精英受体DNA最大化。

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