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1. 发明申请

US20130109578A1 DIFFERENTIATION OF ISOBARIC AMINO ACIDS AND OTHER SPECIES 有权
标题翻译： ISOBARIC氨基酸和其他物种的差异
公开(公告)号：US20130109578A1
公开(公告)日：2013-05-02
申请号：US13386333
申请日：2009-07-22
申请人： James R. Heath , Su Seong Lee , Jaehong Lim , Junhoe Cha , Sylvia Tan , Shi Yun Yeo
发明人： James R. Heath , Su Seong Lee , Jaehong Lim , Junhoe Cha , Sylvia Tan , Shi Yun Yeo
IPC分类号： G01N33/68 , C07K1/13
CPC分类号： G01N33/6848 , C07B2200/11 , C07K1/047 , C07K1/13 , C07K7/06 , C40B50/16 , G01N33/6812 , G01N33/6818
摘要： Techniques for differentiating isobaric species are described. An isobaric species may be substituted with a tagging species identified using mass spectrometry. The isobaric species may be a subunit of a first polymer having a defined sequence, e.g., the isobaric species may be an amino acid in a protein or a peptide sequence. A tagging species may be substituted for the isobaric species in a second polymer having an otherwise identical sequence as the first polymer. The second polymer may have the same number of sequences as the first polymer, and substantially the same sequence of subunits, with a few exceptions such as the tagging species for the isobaric species. The first polymer and the second polymer may be prepared in the same reaction vessel. A polymer/protein of defined subunit sequence containing an isobaric species or a tagging species may be analyzed by mass spectrometry to determine the sequence.
摘要翻译：描述了用于区分等压物种的技术。同量异位物质可以用用质谱鉴定的标记物种代替。等压异构物可以是具有确定的序列的第一聚合物的亚单位，例如，同量异位物质可以是蛋白质或肽序列中的氨基酸。在具有与第一聚合物相同的序列的第二聚合物中，标记物质可以代替同量异位物质。第二聚合物可以具有与第一聚合物相同数量的序列，以及基本上相同的亚基序列，只有少数例外，例如同量异序物种的标记物种。第一聚合物和第二聚合物可以在相同的反应容器中制备。可以通过质谱分析含有等压物种或标记物质的限定亚单位序列的聚合物/蛋白质来确定序列。

2. 发明申请

US20120122711A1 SCREENING OF BIOPOLYMERS 审中-公开
标题翻译：生物聚合物筛选
公开(公告)号：US20120122711A1
公开(公告)日：2012-05-17
申请号：US13384510
申请日：2010-07-15
申请人： James R. Heath , Su Seong Lee , Jaehong Lim , Junhoe Cha , Sylvia Tan , Shi Yun Yeo , Yi Li Ang , Yiran Zhang
发明人： James R. Heath , Su Seong Lee , Jaehong Lim , Junhoe Cha , Sylvia Tan , Shi Yun Yeo , Yi Li Ang , Yiran Zhang
IPC分类号： C40B30/00 , C40B50/18 , C40B40/12 , C40B40/06 , C40B40/10
CPC分类号： G01N33/6845
摘要： Described herein are inventive compositions and methods relating to sampling of biopolymers and, in particular, to fractional sampling of biopolymers. In one aspect, embodiments are generally related to unique biopolymer species where a fraction of each biopolymer species contains a cleavable linker. The biopolymer species may, in some embodiments, be attached to a surface. For example, the biopolymer species may be attached to beads. In some embodiments, a portion of a unique biopolymer species may be sampled by cleaving the cleavable linker. In some cases, the sample may be analyzed to determine the sequence of the biopolymer.
摘要翻译：本文描述了与生物聚合物的取样有关的本发明组合物和方法，特别是生物聚合物的分数取样。在一个方面，实施方案通常涉及独特的生物聚合物种类，其中每个生物聚合物种类的一部分含有可切割的连接体。在一些实施方案中，生物聚合物物质可附着于表面。例如，生物聚合物物质可以连接到珠粒上。在一些实施方案中，可以通过切割可切割接头来采样一部分独特的生物聚合物种类。在一些情况下，可以分析样品以确定生物聚合物的顺序。

3. 发明授权

US09863956B2 Differentiation of isobaric amino acids and other species 有权
公开(公告)号：US09863956B2
公开(公告)日：2018-01-09
申请号：US13386333
申请日：2009-07-22
申请人： James R. Heath , Su Seong Lee , Jaehong Lim , Junhoe Cha , Sylvia Tan , Shi Yun Yeo
发明人： James R. Heath , Su Seong Lee , Jaehong Lim , Junhoe Cha , Sylvia Tan , Shi Yun Yeo
IPC分类号： G01N33/68 , C07K1/04 , C07K7/06 , C40B50/16 , C07K1/13
CPC分类号： G01N33/6848 , C07B2200/11 , C07K1/047 , C07K1/13 , C07K7/06 , C40B50/16 , G01N33/6812 , G01N33/6818
摘要： Techniques for differentiating isobaric species are described. An isobaric species may be substituted with a tagging species identified using mass spectrometry. The isobaric species may be a subunit of a first polymer having a defined sequence, e.g., the isobaric species may be an amino acid in a protein or a peptide sequence. A tagging species may be substituted for the isobaric species in a second polymer having an otherwise identical sequence as the first polymer. The second polymer may have the same number of sequences as the first polymer, and substantially the same sequence of subunits, with a few exceptions such as the tagging species for the isobaric species. The first polymer and the second polymer may be prepared in the same reaction vessel. A polymer/protein of defined subunit sequence containing an isobaric species or a tagging species may be analyzed by mass spectrometry to determine the sequence.

4. 发明申请

US20130267423A1 PEPTIDE LIBRARIES FOR SCREENING AND OTHER APPLICATIONS 审中-公开
标题翻译：用于筛选和其他应用的肽图书馆
公开(公告)号：US20130267423A1
公开(公告)日：2013-10-10
申请号：US13819534
申请日：2011-08-29
申请人： Su Seong Lee , Jaehong Lim , Junhoe Cha , Yi Li Ang , Yiran Zheng , Sylvia Tan
发明人： Su Seong Lee , Jaehong Lim , Junhoe Cha , Yi Li Ang , Yiran Zheng , Sylvia Tan
IPC分类号： G01N33/68
CPC分类号： G01N33/6818 , C07K1/047 , C07K1/13 , C07K7/06 , C40B20/08 , C40B40/04 , C40B50/18 , C40B70/00
摘要： The present invention generally relates to various peptides and particles, for example, for use in screening of particle- or bead-based peptide libraries. In one aspect, the present invention is generally directed to articles including peptides attached to one or more particles, which may have structures such as (particle)-M-Q-Zn-X-J, (particle)-M-Q-Zn—X1—X2—X3—X4—X5-J, (particle)-M-R—Zn—X1—X2—X3—X4—X5-J, (particle)-M-R—X1—X2—X3—X4—X5Zn-Q-J, (particle)-M-X1—X2—X—3X4—X5—Zn—R-J, etc., where M is a methionine residue, M1 is a cleavable linker residue, Q is a group able to enhance intensity and/or sensitivity of mass spectrometry, X comprises one or more amino acid residues, n is a positive integer, Z is a covalent bond or a spacer, and J is an end-group. In some embodiments, the spacer may comprise a structure such as:(I). Other aspects of the present invention generally relate to methods of using such articles, e.g., by exposing the article to a target molecule such as a protein, for example, for use in screening of particle-based peptide libraries. Still other aspects of the present invention generally relate to methods of making such articles, methods of promoting such articles, kits involving such articles, or the like.
摘要翻译：本发明通常涉及各种肽和颗粒，例如用于筛选基于颗粒或珠的肽文库。一方面，本发明一般涉及包含连接到一个或多个颗粒上的肽的制品，其可以具有诸如（颗粒）-MQ-Zn-XJ，（颗粒）-MQ-Zn-X1-X2-X3 -X4-X5-J，（颗粒）-MR-Zn-X1-X2-X3-X4-X5-J，（颗粒）-MR-X1-X2-X3-X4-X5Zn-QJ，（颗粒） -X1-X2-X-3X4-X5-Zn-RJ等，其中M是甲硫氨酸残基，M1是可切割的接头残基，Q是能够提高质谱强度和/或灵敏度的基团，X包括一个或多个氨基酸残基，n为正整数，Z为共价键或间隔基，J为端基。在一些实施方案中，间隔物可以包括如下结构：（I）。本发明的其它方面通常涉及使用这种制品的方法，例如通过将制品暴露于靶分子例如蛋白质，例如用于筛选基于粒子的肽文库。本发明的其它方面通常涉及制造这种制品的方法，促进这种制品的方法，涉及这种制品的试剂盒等。

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