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1. 发明申请

WO2008142707A2 INSECTICIDAL XNGROEL PROTEIN OF XENORHABDUS NEMATOPHILA 审中-公开
标题翻译： XENORHABDUS NEMATOPHILA的杀虫剂XNGROEL蛋白质
公开(公告)号：WO2008142707A2
公开(公告)日：2008-11-27
申请号：PCT/IN2008000316
申请日：2008-05-19
申请人： JOSHI MOHAN CHANDRA , SHARMA ANIMESH , BIRAH AJANTA , GUPTA GORAKH PRASAD , BANERJEE NIRUPAMA , BHATNAGAR RAKESH
发明人： JOSHI MOHAN CHANDRA , SHARMA ANIMESH , BIRAH AJANTA , GUPTA GORAKH PRASAD , BANERJEE NIRUPAMA , BHATNAGAR RAKESH
IPC分类号： A01N63/00
CPC分类号： A01N63/00 , A01N63/02 , C07K14/24
摘要： The present invention relates to a novel insecticidal protein named XnGroEL isolated and purified from Xenorhabdus nematophila having GENE BANK ACCESSION No. AY18449. The process for the purification of novel insecticidal protein named XnGroEL is also disclosed. The invention also relates to the process for preparation and purification of the recombinant novel insecticidal XnGroEL protein. The process involves purification of a novel insecticidal protein Xenorhabdus nematophila, construction of genomic DNA library, screening of library using probes with N-terminal amino acid sequence, cloning in an expression vector followed by its transformation in E. coli cells and purification of the recombinant protein. Both the novel native and recombinant insecticidal XnGroEL protein have growth inhibitory and insecticidal property for Helico?erpa armigera larvae. The present invention also discloses that the novel native and recombinant insecticidal XnGroEL protein has larval gut brush border membrane vesicles binding activity and a-chitin binding activity.
摘要翻译：本发明涉及一种名叫XnGroEL的新型杀虫蛋白，其由具有GENE BANK ACCESSION AY18449的Xenorhabdus nematophila分离和纯化。还公开了用于纯化名为XnGroEL的新型杀虫蛋白的方法。本发明还涉及重组新型杀虫XnGroEL蛋白的制备和纯化方法。该方法包括纯化新型杀虫蛋白Xenorhabdus nematophila，构建基因组DNA文库，使用具有N-末端氨基酸序列的探针筛选文库，在表达载体中克隆，随后在大肠杆菌细胞中转化并纯化重组体蛋白。新型天然和重组杀虫XnGroEL蛋白都具有对Helico？erpa armigera幼虫的生长抑制和杀虫性能。本发明还公开了新型天然和重组杀虫XnGroEL蛋白具有幼虫肠刷状膜囊泡结合活性和α-壳多糖结合活性。

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