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1. 发明公开

EP1958960A2 A process for the preparation of a non-toxic anthrax vaccine 审中-公开
标题翻译： Herstellung einer nicht-toxischen Anthrax-Impfung的Verfahrenfür
公开(公告)号：EP1958960A2
公开(公告)日：2008-08-20
申请号：EP08006580.8
申请日：2002-03-20
申请人： Bhatnagar, Rakesh
发明人： The designation of the inventor has not yet been filed
IPC分类号： C07K14/32 , C12N15/01
CPC分类号： C07K14/32 , A61K39/00
摘要： Anthrax toxin, comprising of protective antigen (PA), lethal factor (LF) and edema factor (EF) is a major virulent factor of B. anthracis. Protective antigen, PA is the main component of all the vaccines against anthrax. The protective efficacy of PA is greatly increased if small quantities of LF or EF are incorporated into the vaccines. An ideal vaccine against anthrax should contain PA, LF and EF together, but this combination would be toxic. Therefore, the biologically inactive mutant preparations of PA, LF and EF may be used together for better immunoprotection. The present invention describes the method for generation of recombinant vaccine against anthrax, comprising of non-toxic, mutant anthrax toxin proteins. The procedure involves site-directed mutagenesis of the native genes of the toxin proteins, the expression and purification of the mutant proteins and finally characterization of these proteins.
摘要翻译：包含保护性抗原（PA），致死因子（LF）和水肿因子（EF）的炭疽毒素是炭疽杆菌的主要毒性因子。保护性抗原，PA是所有针对炭疽疫苗的主要成分。如果将少量的LF或EF并入疫苗中，则PA的保护功效大大增加。一种针对炭疽的理想疫苗应包含PA，LF和EF，但这种组合是有毒的。因此，PA，LF和EF的生物活性突变体制剂可以一起用于更好的免疫保护。本发明描述了用于产生针对炭疽的重组疫苗的方法，其包含无毒的突变体炭疽毒素蛋白质。该过程涉及毒素蛋白质的天然基因的定点突变，突变蛋白质的表达和纯化以及这些蛋白质的最终表征。

2. 发明公开

EP1442052A1 HIGH LEVEL CONSTITUTIVE PRODUCTION OF ANTHRAX PROTECTIVE ANTIGEN 有权
标题翻译： HEIGHT炭疽构成生产表面保护抗原的
公开(公告)号：EP1442052A1
公开(公告)日：2004-08-04
申请号：EP01274653.3
申请日：2001-12-07
申请人： Bhatnagar, Rakesh , Waheed, Syed Moshin , Chauhan, Vibha
发明人： Bhatnagar, Rakesh , Waheed, Syed Moshin , Chauhan, Vibha
IPC分类号： C07K14/32
CPC分类号： C07K14/32
摘要： The present invention relates to a process for preparing anthrax protective antigen protein from E. coli using fed batch culture. This process creates a constitutively expressing system for rapid, efficient, cost-effective and high-level production of anthrax PA from E. coli. The steps of the process involves, transforming E. coli DH5alpha cells with the recombinant constitutive expression plasmid containing the PA gene to obtain recombinant DH5alpha cells and testing the PA expression by lysis of said cells followed by denaturing gel electrophoresis and Western Blotting technique using PA antibodies. This is followed by fermentation and harvesting of the high cell density cells. The said cells are solubilized using 6-8 Molar Urea and separated by centrifugation. The urea denatured PA is isolated from said supernatant and purified and thereafter eluted.

3. 发明专利

AU2002242935A8 A process for the preparation of a non-toxic anthrax vaccine 未知
公开(公告)号：AU2002242935A8
公开(公告)日：2003-06-17
申请号：AU2002242935
申请日：2002-03-20
申请人： BHATNAGAR RAKESH
发明人： SINGH APARNA , BATRA SMRITI , GUPTA PANKAJ , CHAUHAN VIBHA , AHUJA NIDHI , KUMAR PRAVEEN , BHATNAGAR RAKESH
IPC分类号： C12N15/09 , A61K38/00 , A61K39/00 , A61K39/07 , A61P31/04 , A61P37/02 , C07K2/00 , C07K14/32 , C07K19/00 , C12N1/21 , C12N15/31 , C12N15/70 , C12P21/02

4. 发明专利

CA2465679A1 HIGH LEVEL CONSTITUTIVE PRODUCTION OF ANTHRAX PROTECTIVE ANTIGEN 未知
公开(公告)号：CA2465679A1
公开(公告)日：2003-05-15
申请号：CA2465679
申请日：2001-12-07
申请人： BHATNAGAR RAKESH , WAHEED MOHSIN SYED , CHAUHAN VIBHA
发明人： BHATNAGAR RAKESH , WAHEED MOHSIN SYED , CHAUHAN VIBHA
IPC分类号： C12P21/02 , A61K39/07 , A61P31/04 , C07K20060101 , C07K14/32 , C12N1/21 , C12P21/00 , C12R1/07
摘要： The present invention relates to a process for preparing anthrax protective antigen protein from E.coli using fed batch culture. This process creates a constitutively expressing system for rapid, efficient, cost-effective and hi gh- level production of anthrax PA from E.coli. The steps of the process involve s, transforming E.coli DH5.alpha. cells with the recombinant constitutive expression plasmid containing the PA gene to obtain recombinant DH5.alpha. cells and testing the PA expression by lysis of said cells followed by denaturing gel electrophoresis and Western Blotting technique using PA antibodies. This is followed by fermentation and harvesting of the high cell density cells. The said cells are solubilized using 6-8 Molar Urea and separated by centrifugation. The urea denatured PA is isolated from said supernatant and purified and thereafter eluted.

5. 发明授权

EP1442052B1 HIGH LEVEL CONSTITUTIVE PRODUCTION OF ANTHRAX PROTECTIVE ANTIGEN 有权
标题翻译： HEIGHT炭疽构成生产表面保护抗原的
公开(公告)号：EP1442052B1
公开(公告)日：2010-10-13
申请号：EP01274653.3
申请日：2001-12-07
申请人： Bhatnagar, Rakesh
发明人： Bhatnagar, Rakesh , Waheed, Syed Moshin , Chauhan, Vibha
IPC分类号： C07K14/32
CPC分类号： C07K14/32
摘要： The present invention relates to a process for preparing anthrax protective antigen protein from E. coli using fed batch culture. This process creates a constitutively expressing system for rapid, efficient, cost-effective and high-level production of anthrax PA from E. coli. The steps of the process involves, transforming E. coli DH5alpha cells with the recombinant constitutive expression plasmid containing the PA gene to obtain recombinant DH5alpha cells and testing the PA expression by lysis of said cells followed by denaturing gel electrophoresis and Western Blotting technique using PA antibodies. This is followed by fermentation and harvesting of the high cell density cells. The said cells are solubilized using 6-8 Molar Urea and separated by centrifugation. The urea denatured PA is isolated from said supernatant and purified and thereafter eluted.

6. 发明公开

EP1958960A3 A process for the preparation of a non-toxic anthrax vaccine 审中-公开
标题翻译：一种用于非毒性炭疽疫苗的制备方法
公开(公告)号：EP1958960A3
公开(公告)日：2009-01-07
申请号：EP08006580.8
申请日：2002-03-20
申请人： Bhatnagar, Rakesh
发明人： Bhatnagar, Rakesh , Gupta, Pankaj , Batra, Smriti , Chauhan, Vibha , Singh, Aparna , Ahuja, Nidhi , Kumar, Praveen
IPC分类号： C07K14/32 , C12N15/01
CPC分类号： C07K14/32 , A61K39/00
摘要： Anthrax toxin, comprising of protective antigen (PA), lethal factor (LF) and edema factor (EF) is a major virulent factor of B. anthracis. Protective antigen, PA is the main component of all the vaccines against anthrax. The protective efficacy of PA is greatly increased if small quantities of LF or EF are incorporated into the vaccines. An ideal vaccine against anthrax should contain PA, LF and EF together, but this combination would be toxic. Therefore, the biologically inactive mutant preparations of PA, LF and EF may be used together for better immunoprotection. The present invention describes the method for generation of recombinant vaccine against anthrax, comprising of non-toxic, mutant anthrax toxin proteins. The procedure involves site-directed mutagenesis of the native genes of the toxin proteins, the expression and purification of the mutant proteins and finally characterization of these proteins.

7. 发明公开

EP1453534A1 A PROCESS FOR THE PREPARATION OF A NON-TOXIC ANTHRAX VACCINE 审中-公开
标题翻译：用于生产无毒炭疽疫苗
公开(公告)号：EP1453534A1
公开(公告)日：2004-09-08
申请号：EP02708609.9
申请日：2002-03-20
申请人： Bhatnagar, Rakesh
发明人： BHATNAGAR, Rakesh,Centre of Biotechnology , GUPTA, Pankaj,Centre For Biotechnology , BATRA, Smriti,Centre For Biotechnology , CHAUHAN, Vibha,Centre of Biotechnology , SINGH, Aparna,Centre For Biotechnology , AHUJA, Nidhi,Centre For Biotechnology , KUMAR, Praveen,Centre For Biotechnology
IPC分类号： A61K39/07 , C07K14/32
CPC分类号： C07K14/32 , A61K39/00
摘要： Anthrax toxin, comprising of protective antigen (PA), lethal factor (LF) and edema factor (EF) is a major virulent factor of B.anthracis. Protective antigen, PA is the main component of all the vaccines against anthrax. The protective efficacy of PA is greatly increased if small quantities of LF or EF are incorporated into the vaccines. An ideal vaccine against anthrax should contain PA, LF and EF together, but this combination would be toxic. Therefore, the biologically inactive mutant preparations of PA, LF and EF may be used together for better immunoprotection. The present invention describes the method for generation of recombinant vaccine against anthrax, comprising of non-toxic, mutant anthrax toxin proteins. The procedure involves site-directed mutagenesis of the native genes of the toxin proteins, the expression and purification of the mutant proteins and finally characterization of these proteins.

8. 发明专利

MXPA04005538A PROCEDIMIENTO PARA LA PREPARACION DE UNA VACUNA NO TOXICA CONTRA EL ANTRAX. 未知
公开(公告)号：MXPA04005538A
公开(公告)日：2005-06-08
申请号：MXPA04005538
申请日：2002-03-20
申请人： BHATNAGAR RAKESH
发明人： GUPTA PANKAJ
IPC分类号： C12N15/09 , A61K38/00 , A61K39/00 , A61K39/07 , A61P31/04 , A61P37/02 , C07K2/00 , C07K14/32 , C07K19/00 , C12N1/21 , C12N15/31 , C12N15/70 , C12P21/02 , C12Q1/68
摘要： La toxina de antrax que comprende de antigeno protector (PA), factor letal (LF) y factor de edema (RF) es un factor principal virulento de B. anthracis; el antigeno protector PA es el principal componente de toas las vacunas contra el antrax; al eficacia protectora de PA es incrementada grandemente si se incorporan pequenas cantidades de LF o EF en las vacunas; una vacuna ideal contra el antrax debe contener PA, LE o EF en conjunto, pero esta combinacion puede ser toxica, por tanto, las preparaciones mutantes biologicamente inactivas de PA, LF y EF se pueden usar en conjunto para una inmunoproteccion mejor; la presente invencion describe el metodo para la generacion de una vacuna recombinante contra antrax, que comprende proteinas de toxina de antrax, mutantes, no toxicas; el procedimiento involucra mutagenesis dirigida al sitio de los genes nativo de las proteinas de toxina, la expresion y purificacion de las proteinas mutantes y finalmente la caracterizacion de estas proteinas.

9. 发明专利

IN190590B A process for constitutively preparing anthrax protective antigen 未知
公开(公告)号：IN190590B
公开(公告)日：2003-08-09
申请号：IN1127DE2001
申请日：2001-11-05
申请人： BHATNAGAR RAKESH , WAHEED SYED MOHSIN , CHAUHAN VIBHA
发明人： BHATNAGAR RAKESH , WAHEED SYED MOHSIN , CHAUHAN VIBHA
IPC分类号： C12P21/02 , A61K39/07 , A61P31/04 , C07K20060101 , C07K14/32 , C12N1/21 , C12P21/00 , C12R1/07 , A61K39/00 , A61P43/00
摘要： The present invention relates to a process for preparing anthrax protective antigen protein from E. coli using fed batch culture. This process creates a constitutively expressing system for rapid, efficient, cost-effective and high-level production of anthrax PA from E. coli. The steps of the process involves, transforming E. coli DH5alpha cells with the recombinant constitutive expression plasmid containing the PA gene to obtain recombinant DH5alpha cells and testing the PA expression by lysis of said cells followed by denaturing gel electrophoresis and Western Blotting technique using PA antibodies. This is followed by fermentation and harvesting of the high cell density cells. The said cells are solubilized using 6-8 Molar Urea and separated by centrifugation. The urea denatured PA is isolated from said supernatant and purified and thereafter eluted.

10. 发明专利

AU2002242935A1 A PROCESS FOR THE PREPARATION OF A NON-TOXIC ANTHRAX VACCINE 未知
公开(公告)号：AU2002242935A1
公开(公告)日：2003-06-17
申请号：AU2002242935
申请日：2002-03-20
申请人： BHATNAGAR RAKESH
发明人： GUPTA PANKAJ , BATRA SMRITI , CHAUHAN VIBHA , SINGH APARNA , AHUJA NIDHI , KUMAR PRAVEEN , BHATNAGAR RAKESH
IPC分类号： C12N15/09 , A61K38/00 , A61K39/00 , A61K39/07 , A61P31/04 , A61P37/02 , C07K2/00 , C07K14/32 , C07K19/00 , C12N1/21 , C12N15/31 , C12N15/70 , C12P21/02 , C12Q1/68

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