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1. 发明授权

US4090919A Water-insoluble tannin preparation for immobilization of proteins 失效
标题翻译：用于固定蛋白质的水不溶性单宁制剂
公开(公告)号：US4090919A
公开(公告)日：1978-05-23
申请号：US760441
申请日：1977-01-19
申请人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Taizo Watanabe , Ryujiro Sano , Yuhsi Matuo
发明人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Taizo Watanabe , Ryujiro Sano , Yuhsi Matuo
IPC分类号： B01J20/32 , C07H13/12 , C07H15/04 , C07H15/08 , C12N11/02 , C07G7/02 , C07C69/76 , C07G7/00
CPC分类号： C07H13/12 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07H15/04 , C07H15/08 , C12N11/02 , Y10S435/803 , Y10S435/815 , Y10S530/813 , Y10S530/816
摘要： A water-insoluble tannin preparation is obtained by covalent binding or physical adsorption of tannin onto a water-insoluble, hydrophilic carrier. The preparation has a specific affinity for proteins and can be used as an adsorbent for purification, isolation and/or separation of proteins (e.g., Enzymes, albumin, globulin, hormonal proteins) from a mixture of compounds. Further, the water-insoluble tannin preparation having a catalytically active enzyme absorbed thereon can be used as a heterogeneous catalyst to induce enzymatic reactions.
摘要翻译：通过将单宁进行共价结合或物理吸附到不溶于水的亲水载体上获得水不溶性单宁制剂。该制剂对蛋白质具有特异性亲和力，可用作用于从化合物混合物中纯化，分离和/或分离蛋白质（例如酶，白蛋白，球蛋白，激素蛋白质）的吸附剂。此外，具有吸附在其上的催化活性酶的水不溶性单宁制剂可以用作促进酶反应的多相催化剂。

2. 发明授权

US4224411A Immobilized aminoacylase 失效
标题翻译：固定氨基酰化酶
公开(公告)号：US4224411A
公开(公告)日：1980-09-23
申请号：US911995
申请日：1978-06-02
申请人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Taizo Watanabe , Motoki Fujimura
发明人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Taizo Watanabe , Motoki Fujimura
IPC分类号： C12N11/10 , C12N11/08 , C12P13/04 , C12N11/02
CPC分类号： C12P13/04 , C12N11/08
摘要： An immobilized aminoacylase preparation is obtained by adsorbing tannin on a water-insoluble, hydrophilic carrier to give a water-insoluble tannin preparation, adsorbing aminoacylase on the tannin preparation to give a water-insoluble tannin-aminoacylase preparation, and then binding ferric or ferrous ion thereto. The immobilized preparation obtained above sustains a high rate of enzymatic activity for a long period of time when used in an enzymatic reaction thereof with N-acyl-DL-amino acids.
摘要翻译：通过将单宁吸附在不溶于水的亲水性载体上得到固定化的氨基酰化酶制剂，得到不溶于水的单宁制剂，在单宁制剂上吸附氨基酰化酶，得到不溶于水的单宁 - 氨基酰化酶制剂，然后结合铁或亚铁离子到此。当与N-酰基-DL-氨基酸的酶反应中使用时，上述获得的固定化制剂在长时间内维持高速率的酶活性。

3. 发明授权

US3935072A Purification of coenzyme A 失效
标题翻译：辅酶A的纯化
公开(公告)号：US3935072A
公开(公告)日：1976-01-27
申请号：US384212
申请日：1973-07-31
申请人： Ichiro Chibata , Tetsuya Tosa , Yuhsi Matuo
发明人： Ichiro Chibata , Tetsuya Tosa , Yuhsi Matuo
IPC分类号： C12P19/32 , C07G7/02 , C07G3/00 , C07G7/00
CPC分类号： C12P19/32 , Y10S530/825
摘要： Coenzyme A is purified by a process involving contacting a cyanogen halide-activated water-insoluble polysaccharide with a cell-free extract of a coenzyme A-producing microorganism to immobilize proteins such as enzymes having affinity for coenzyme A on the polysaccharide. The immobilized proteins are then contacted with a coenzyme A-containing solution to absorb coenzyme A followed by eluting coenzyme A from the immobilized proteins. Alternatively, the proteins having affinity for coenzyme A may be isolated from the cell-free extract prior to immobilization by absorbing the proteins on coenzyme A immobilized on a cyanogen halide-activated water-insoluble polysaccharide followed by eluting the proteins from the immobilized coenzyme A.
摘要翻译：辅酶A通过使卤化氰活化的水不溶性多糖与产生辅酶A的微生物的无细胞提取物接触的方法进行纯化，从而将对辅酶A具有亲和性的酶等蛋白质固定在多糖上。然后将固定的蛋白质与含辅酶A的溶液接触以吸收辅酶A，然后从固定化蛋白质洗脱辅酶A. 或者，可以通过将固定在氰卤化物活化的水不溶性多糖上的辅酶A上的蛋白质吸收固定化，然后从固定化的辅酶A洗脱蛋白质，在固定前从无细胞提取物中分离出对辅酶A具有亲和力的蛋白质。

4. 发明授权

US4390626A Immobilized aminoacylase 失效
标题翻译：固定氨基酰化酶
公开(公告)号：US4390626A
公开(公告)日：1983-06-28
申请号：US288170
申请日：1981-07-29
申请人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Motoki Fujimura
发明人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Motoki Fujimura
IPC分类号： C12N11/02 , C12N11/06 , C12N11/08 , C12N11/14 , C12P13/04
CPC分类号： C12N11/14 , C12N11/08
摘要： An immobilized aminoacylase is prepared by bonding aminoacylase to a water-insoluble porous anion exchanger such as a porous phenolic resin, porous styrene resin, porous silica or porous glass having anion exchange groups and treating the bonded amino-acylase with a crosslinking agent such as an aliphatic dialdehyde. The porous anion exchanger preferably has a pore size of about 150.degree. to 3,000 A.degree., pore volume of about 0.3 to 1.0 m.sup.1 /g, specific surface area of about 10 to 150 m.sup.2 /g and particle size of above 0.1 to 1.2 mm. Preferred anion exchangers are trimethylammonium-introduced styrene resin and trimethylammonium-introduced silica.
摘要翻译：通过将氨基酰化酶结合到诸如多孔酚醛树脂，多孔苯乙烯树脂，多孔二氧化硅或具有阴离子交换基团的多孔玻璃之类的水不溶性多孔阴离子交换剂上并用交联剂处理粘合的氨基酰化酶来制备固定化的氨基酰化酶，脂肪族二醛。多孔阴离子交换剂的孔径优选为约150〜3000A，孔体积为约0.3〜1.0m1 / g，比表面积为约10〜150m2 / g，粒径为0.1〜1.2mm以上。优选的阴离子交换剂是引入三甲基铵的苯乙烯树脂和引入三甲基铵的二氧化硅。

5. 发明授权

US4381239A Method for reducing the pyrogen content of or removing pyrogens from substances contaminated therewith 失效
标题翻译：降低热原体含量或从其污染的物质中除去热原的方法
公开(公告)号：US4381239A
公开(公告)日：1983-04-26
申请号：US343269
申请日：1982-01-27
申请人： Ichiro Chibata , Tetsuya Tosa , Tadashi Sato , Taizo Watanabe , Satoshi Minobe
发明人： Ichiro Chibata , Tetsuya Tosa , Tadashi Sato , Taizo Watanabe , Satoshi Minobe
IPC分类号： A61K9/08 , A61K9/00 , A61K9/18 , B01D15/00 , B01D15/08 , B01J20/26 , B01J20/32 , C08B11/14 , C08B37/00 , C08B37/12
CPC分类号： A61K9/0019 , B01D15/08 , B01J20/3255 , C08B11/14 , C08B37/0039
摘要： A method for removing a pyrogen from a solution of a pyrogen-containing substance such as an amino acid, a nucleic acid base, an antibiotic, a hormone, a vitamin, an enzyme, an antibody or the like comprising contacting the solution with an adsorbent to adsorb the pyrogen, which is characterized in that the adsorbent comprises a water-insoluble carrier and a nitrogen-containing heterocyclic compound of the formula:R--A--Xwherein R is a nitrogen-containing heterocyclic group; A is single bond, alkylene or alkenylene; X is hydrogen or a functional group; and the heterocyclic group and alkylene may be optionally substituted by one or more substituents, and the compound being bonded to the carrier directly or through a spacer.
摘要翻译：一种从含热原物质如氨基酸，核酸碱基，抗生素，激素，维生素，酶，抗体等的溶液中除去热原的方法，包括使该溶液与吸附剂吸附热原，其特征在于吸附剂包括水不溶性载体和式RAX的含氮杂环化合物，其中R是含氮杂环基; A是单键，亚烷基或亚烯基; X是氢或官能团; 并且杂环基和亚烷基可以任选地被一个或多个取代基取代，并且化合物直接或通过间隔基键合到载体上。

6. 发明授权

US3963613A Blood purification means 失效
标题翻译：血液净化手段
公开(公告)号：US3963613A
公开(公告)日：1976-06-15
申请号：US536641
申请日：1974-12-26
申请人： Ichiro Chibata , Tetsuya Tosa , Takao Mori
发明人： Ichiro Chibata , Tetsuya Tosa , Takao Mori
IPC分类号： A61F2/02 , A61M1/16 , A61M1/36 , B01D31/00
CPC分类号： A61F2/022 , A61M1/1696 , A61M1/3687
摘要： A blood purification means whereby blood of a patient to be treated is led around an external circuit connecting to the patient's blood stream and is brought into direct or indirect contact with a fumarate solution, and is further brought into direct or indirect contact with an enzymic preparation, which is suitably an aspartase preparation, which catalyzes a reaction of L-aspartic acid formation from fumaric acid and ammonia. The purification means may further include a preliminary stage or stages whereat an unrequired substance in a patient's blood is decomposed to a non toxic substance and ammonia subsequently convertable to aspartic acid, and may also include a low molecular sieve means preventing re-entry of aspartic acid produced into the patient's blood stream.
摘要翻译：一种血液净化装置，其特征在于，将待治疗的患者的血液引导到连接患者血液流的外部回路，并与富马酸盐溶液直接或间接接触，并进一步与酶制剂直接或间接接触其合适地是天冬氨酰胺酶制剂，其催化由富马酸和氨形成的L-天冬氨酸的反应。净化装置还可以包括预备阶段或阶段，其中将患者血液中的不需要物质分解成无毒物质，然后氨转化成天冬氨酸，还可以包括防止天冬氨酸再次进入的低分子筛产生到患者的血液中。

7. 发明授权

US4081327A Preparation of d-fructose 失效
标题翻译： D-果糖的制备
公开(公告)号：US4081327A
公开(公告)日：1978-03-28
申请号：US461509
申请日：1974-04-17
申请人： Ichiro Chibata , Tetsuya Tosa , Tadashi Sato
发明人： Ichiro Chibata , Tetsuya Tosa , Tadashi Sato
IPC分类号： C12N11/04 , C12N11/08 , C12P19/24 , C12D13/00 , C07G7/02 , C12K1/00
CPC分类号： C12P19/24 , C12N11/04 , Y10S435/832 , Y10S435/89 , Y10S435/897 , Y10S435/902
摘要： An immobilized glucose isomerase-producing microorganism is reacted with D-glucose to produce D-fructose. Immobilization is carried out by polymerizing at least one acryloyl monomer in an aqueous suspension containing a glucose isomerase-producing microorganism. The acryloyl monomers employed include acryloylamide, N,N'-lower alkylene-bis-acryloylamide, bis(acryloylamidomethyl)ether and N,N'-di-acryloyl-ethyleneurea.
摘要翻译：产生固定葡萄糖异构酶的微生物与D-葡萄糖反应产生D-果糖。通过在含有产生葡萄糖异构酶的微生物的水性悬浮液中聚合至少一种丙烯酰基单体进行固定。所用的丙烯酰基单体包括丙烯酰胺，N，N'-低级亚烷基 - 双丙烯酰胺，双（丙烯酰氨基甲基）醚和N，N'-二丙烯酰基亚乙基脲。

8. 发明授权

US4138292A Immobilized catalytically active substance and method of preparing the same 失效
标题翻译：固定催化活性物质及其制备方法
公开(公告)号：US4138292A
公开(公告)日：1979-02-06
申请号：US807259
申请日：1977-06-16
申请人： Ichiro Chibata , Tetsuya Tosa , Isao Takata
发明人： Ichiro Chibata , Tetsuya Tosa , Isao Takata
IPC分类号： C12N11/04 , C12N11/10 , C07G7/02 , C12B1/00
CPC分类号： C12N11/10 , C12N11/04 , Y10S435/824 , Y10S435/839 , Y10S435/84 , Y10S435/849 , Y10S435/859 , Y10S435/873 , Y10S435/874 , Y10S435/875 , Y10S435/881 , Y10S435/897
摘要： An enzyme or microorganism is entrapped within the gel matrix of a sulfated polysaccharide (said polysaccharide containing more than 10 w/w% of a sulfate moiety in its molecule) in the presence of ammonium ion, a metal ion, a water-soluble amine or a water-miscible organic solvent. The immobilized enzyme or microorganism thus obtained shows a high level of catalytic activity for a long period of time and can be used for continuous enzymatic reactions with substrates.
摘要翻译：在铵离子，金属离子，水溶性胺或其盐的存在下，将酶或微生物包埋在硫酸化多糖（所述分子中含有超过10w / w％的硫酸盐部分的所述多糖）的凝胶基质内水溶性有机溶剂。由此得到的固定化酶或微生物长时间显示高水平的催化活性，可用于与底物的连续酶反应。

9. 发明授权

US3953291A Process for preparing 6-aminopenicillanic acid 失效
标题翻译：制备6-氨基青霉烷酸的方法
公开(公告)号：US3953291A
公开(公告)日：1976-04-27
申请号：US450655
申请日：1974-03-13
申请人： Ichiro Chibata , Tetsuya Tosa , Tadashi Sato
发明人： Ichiro Chibata , Tetsuya Tosa , Tadashi Sato
IPC分类号： C12N11/08 , C07D499/42 , C08F2/44 , C12N11/04 , C12P37/06 , C12D9/00
CPC分类号： C08F2/44 , C12N11/04 , Y10S435/829 , Y10S435/849 , Y10S435/859 , Y10S435/875 , Y10S435/897
摘要： At least one acryloyl monomer is polymerized in an aqueous suspension containing a penicillin amidase-producing microorganism. The acryloyl monomers employed in the present invention include acryloylamide, N,N'-lower alkylene-bis-acryloylamide, bis(acryloylamidomethyl)ether and N,N'-di-acryloylethyleneurea. The resultant immobilized penicillin amidase-producing microorganism is subjected to enzymatic reaction with penicillin. 6-Amino-penicillanic acid is produced.
摘要翻译：至少一种丙烯酰基单体在含有青霉素酰胺酶生产微生物的水性悬浮液中聚合。本发明中使用的丙烯酰基单体包括丙烯酰胺，N，N'-低级亚烷基 - 双丙烯酰胺，双（丙烯酰氨基甲基）醚和N，N'-二丙烯酰亚乙基脲。所得固定的青霉素酰胺酶生产微生物与青霉素进行酶反应。生产6-氨基 - 青霉烷酸。

10. 发明授权

US4433054A Enzymatic active substance immobilized in a polysaccharide gel matrix 失效
标题翻译：固定在多糖凝胶基质中的酶活性物质
公开(公告)号：US4433054A
公开(公告)日：1984-02-21
申请号：US323288
申请日：1981-11-20
申请人： Ichiro Chibata , Tetsuya Tosa , Isao Takata
发明人： Ichiro Chibata , Tetsuya Tosa , Isao Takata
IPC分类号： C12N11/04 , C12N11/10 , C12N11/12
CPC分类号： C12N11/10 , C12N11/04
摘要： An immobilized enzymatic active substance having an improved stability of enzymatic activity is prepared which comprises an enzymatic active substance such as microbial cells entrapped within a gel matrix of an unsubstituted amino, monoalkylamino or dialkylamino group-introduced polysaccharide having not less than 10 w/w % of sulfate moiety in the molecule thereof such as carrageenan. The immobilized enzymatic active substance is prepared by admixing the enzymatic active substance and an aqueous solution of the polysaccharide and then allowing to form a gel of the polysaccharide.
摘要翻译：制备具有改善的酶活性稳定性的固定化的酶活性物质，其包含酶活性物质，例如包含在不小于10w / w％的未取代氨基，单烷基氨基或二烷基氨基引入的多糖的凝胶基质内的微生物细胞，的分子中的硫酸盐部分，例如角叉菜聚糖。通过混合酶活性物质和多糖的水溶液，然后形成多糖的凝胶来制备固定的酶活性物质。

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