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1. 发明授权

US4224411A Immobilized aminoacylase 失效
标题翻译：固定氨基酰化酶
公开(公告)号：US4224411A
公开(公告)日：1980-09-23
申请号：US911995
申请日：1978-06-02
申请人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Taizo Watanabe , Motoki Fujimura
发明人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Taizo Watanabe , Motoki Fujimura
IPC分类号： C12N11/10 , C12N11/08 , C12P13/04 , C12N11/02
CPC分类号： C12P13/04 , C12N11/08
摘要： An immobilized aminoacylase preparation is obtained by adsorbing tannin on a water-insoluble, hydrophilic carrier to give a water-insoluble tannin preparation, adsorbing aminoacylase on the tannin preparation to give a water-insoluble tannin-aminoacylase preparation, and then binding ferric or ferrous ion thereto. The immobilized preparation obtained above sustains a high rate of enzymatic activity for a long period of time when used in an enzymatic reaction thereof with N-acyl-DL-amino acids.
摘要翻译：通过将单宁吸附在不溶于水的亲水性载体上得到固定化的氨基酰化酶制剂，得到不溶于水的单宁制剂，在单宁制剂上吸附氨基酰化酶，得到不溶于水的单宁 - 氨基酰化酶制剂，然后结合铁或亚铁离子到此。当与N-酰基-DL-氨基酸的酶反应中使用时，上述获得的固定化制剂在长时间内维持高速率的酶活性。

2. 发明授权

US4390626A Immobilized aminoacylase 失效
标题翻译：固定氨基酰化酶
公开(公告)号：US4390626A
公开(公告)日：1983-06-28
申请号：US288170
申请日：1981-07-29
申请人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Motoki Fujimura
发明人： Ichiro Chibata , Tetsuya Tosa , Takao Mori , Motoki Fujimura
IPC分类号： C12N11/02 , C12N11/06 , C12N11/08 , C12N11/14 , C12P13/04
CPC分类号： C12N11/14 , C12N11/08
摘要： An immobilized aminoacylase is prepared by bonding aminoacylase to a water-insoluble porous anion exchanger such as a porous phenolic resin, porous styrene resin, porous silica or porous glass having anion exchange groups and treating the bonded amino-acylase with a crosslinking agent such as an aliphatic dialdehyde. The porous anion exchanger preferably has a pore size of about 150.degree. to 3,000 A.degree., pore volume of about 0.3 to 1.0 m.sup.1 /g, specific surface area of about 10 to 150 m.sup.2 /g and particle size of above 0.1 to 1.2 mm. Preferred anion exchangers are trimethylammonium-introduced styrene resin and trimethylammonium-introduced silica.
摘要翻译：通过将氨基酰化酶结合到诸如多孔酚醛树脂，多孔苯乙烯树脂，多孔二氧化硅或具有阴离子交换基团的多孔玻璃之类的水不溶性多孔阴离子交换剂上并用交联剂处理粘合的氨基酰化酶来制备固定化的氨基酰化酶，脂肪族二醛。多孔阴离子交换剂的孔径优选为约150〜3000A，孔体积为约0.3〜1.0m1 / g，比表面积为约10〜150m2 / g，粒径为0.1〜1.2mm以上。优选的阴离子交换剂是引入三甲基铵的苯乙烯树脂和引入三甲基铵的二氧化硅。

3. 发明授权

US4783409A Immobilized enzyme having reversible solubility 失效
标题翻译：具有可逆溶解度的固定化酶
公开(公告)号：US4783409A
公开(公告)日：1988-11-08
申请号：US861158
申请日：1986-05-08
申请人： Tetsuya Tosa , Takao Mori , Motoki Fujimura
发明人： Tetsuya Tosa , Takao Mori , Motoki Fujimura
IPC分类号： C12N11/08 , C12N11/02
CPC分类号： C12N11/08
摘要： An immobilized enzyme having reversible solubility is prepared by attaching an enzyme to a copolymer of two of more monomers selected from the group consisting of acrylic acid or an alkyl ester thereof, methacrylic acid or alkyl ester or dialkylamino-alkyl ester thereof, and a vinylpyridine derivative of the general formula [U]: ##STR1## wherein R is an alkyl. Preferred capolymers are methacrylic acid-alkyl methacrylate-alkyl acrylate, methacrylic acid-alkyl methacrylate, alkyl acrylate-methacrylic acid, alkyl acrylate-methacrylic acid-alkyl acrylate, alkyl acrylate-methacrylic acid-2-methyl-5-vinylpyridine and dialkylaminoalkyl methacrylate-alkyl methacrylate. The immobilized enzyme has a high activity and can dissolve even in an organic solvent or in a solution containing an organic solvent.
摘要翻译：通过将酶连接到选自丙烯酸或其烷基酯，甲基丙烯酸或其烷基酯或其二烷基氨基 - 烷基酯中的两种以上单体的共聚物和乙烯基吡啶衍生物来制备具有可逆溶解度的固定化酶的通式[U]：其中R是烷基。优选的共聚物是甲基丙烯酸 - 甲基丙烯酸烷基酯 - 丙烯酸烷基酯，甲基丙烯酸 - 甲基丙烯酸烷基酯，丙烯酸烷基酯 - 甲基丙烯酸，丙烯酸烷基酯 - 甲基丙烯酸 - 丙烯酸烷基酯，丙烯酸烷基酯 - 甲基丙烯酸-2-甲基-5-乙烯基吡啶，甲基丙烯酸烷基酯。固定化酶具有高活性，即使在有机溶剂或含有机溶剂的溶液中也能溶解。

4. 发明授权

US5968810A Stress-relieving feed 失效
标题翻译：减压饲料
公开(公告)号：US5968810A
公开(公告)日：1999-10-19
申请号：US823201
申请日：1997-03-24
申请人： Motoki Fujimura , Syuhei Ishihara , Kunie Nishijima , Katsuyuki Kataoka
发明人： Motoki Fujimura , Syuhei Ishihara , Kunie Nishijima , Katsuyuki Kataoka
IPC分类号： A23K1/00 , A23K1/18 , A23K1/10 , A01N63/00
CPC分类号： A23K50/00 , A23K10/16
摘要： A feed and a method for relieving stress of livestock and poultry are provided. The feed for relieving stress containing yeast rich in glutathione or a mixture of glutathione and yeast is prepared and is fed to livestock and poultry to relieve their stress.
摘要翻译：提供了减轻牲畜和家禽压力的饲料和方法。制备含有富含谷胱甘肽或谷胱甘肽和酵母混合物的酵母的减轻应力的饲料，并将其喂给牲畜和家禽以减轻其压力。

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