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1. 发明授权

US07052844B2 Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism 失效
公开(公告)号：US07052844B2
公开(公告)日：2006-05-30
申请号：US10269229
申请日：2002-10-11
申请人： Ian J. Atkinson , Glen H. Erikson , Jasmine I. Daksis , Pierre Picard
发明人： Ian J. Atkinson , Glen H. Erikson , Jasmine I. Daksis , Pierre Picard
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6839 , C12Q2563/173 , C12Q2563/107 , C12Q2537/119 , C12Q2527/125 , C12Q2563/137
摘要： A purification method includes bonding a probe to a target in a sample to form a complex, and separating the sample from the complex to separate in a sequence specific manner the target from the sample. The complex, which is immobilized on a support, is a duplex, triplex or quadruplex formed by Watson-Crick complementary base interaction or by homologous base interaction, provided that when the complex is a duplex and the heteropolymeric probe sequence is antiparallel to the heteropolymeric target sequence, the heteropolymeric probe sequence is bonded to the heteropolymeric target sequence by homologous base interaction, and provided that when the complex is a triplex, the complex is free of recombination proteins. A kit for performing the method includes the support and the probe.

2. 发明授权

US06927027B2 Nucleic acid multiplex formation 失效
标题翻译：核酸复制形成
公开(公告)号：US06927027B2
公开(公告)日：2005-08-09
申请号：US09885731
申请日：2001-06-20
申请人： Glen H. Erikson , Jasmine I. Daksis , Ivana Kandic , Pierre Picard
发明人： Glen H. Erikson , Jasmine I. Daksis , Ivana Kandic , Pierre Picard
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/566 , G01N33/58 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q1/6816 , C12Q1/6839 , C12Q2563/173 , C12Q2563/107 , C12Q2527/125 , C12Q2563/137
摘要： Heteropolymeric triplexes and quadruplexes and methods for making them; the use of accelerator agents such as cations to create them; the use of fluorescent intercalators and fluorescent probe-bound non-intercalators to detect them.
摘要翻译：异构复合物和四极体及其制备方法; 使用促进剂如阳离子来形成它们; 使用荧光嵌入剂和荧光探针结合的非嵌入剂来检测它们。

3. 发明授权

US06420115B1 Cation mediated triplex hybridization assay 有权
标题翻译：阳离子介导的三重杂交测定
公开(公告)号：US06420115B1
公开(公告)日：2002-07-16
申请号：US09613263
申请日：2000-07-10
申请人： Glen H. Erikson , Jasmine I. Daksis , Pierre Picard
发明人： Glen H. Erikson , Jasmine I. Daksis , Pierre Picard
IPC分类号： C12Q168
CPC分类号： C12Q1/6839 , C12Q1/6816 , C12Q1/6827 , C12Q2563/137 , C12Q2563/107 , C12Q2563/173
摘要： Triplex complexes contain a single-stranded probe bound to a double-stranded nucleic acid target, in which the probe includes a heteropolymeric nucleic acid or a heteropolymeric nucleic acid analog. All base triplets of the complex are members selected from the group consisting of A-T-A, T-A-T, U-A-T, T-A-U, A-U-A, U-A-U, G-C-G and C-G-C. A cation-facilitated assay includes detecting the presence of such triplex complexes to determine the degree of complementarity between the probe and target sequence. The assay preferably detects a change in fluorescent intensity of a label as a function of binding affinity between the probe and target. The label can be covalently tethered to the probe or to the target, or can be an intercalating fluorophore in the reaction medium.
摘要翻译：三重配合物含有与双链核酸靶结合的单链探针，其中探针包括杂聚核酸或异源核酸类似物。复合体的所有基本三元组是从由A-T-A，T-A-T，U-A-T，T-A-U，A-U-A，U-A-U，G-C-G和C-G-C组成的组中选出的成员。阳离子促进测定法包括检测这种三重配合物的存在以确定探针和靶序列之间的互补程度。该测定优选检测作为探针和靶标之间的结合亲合力的函数的标记物的荧光强度的变化。标记可以共价连接到探针或靶上，或者可以是反应介质中的插层荧光团。

4. 发明授权

US06265170B1 Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions 有权
标题翻译：通过在不同条件下的多次测量，三重杂交的双链体的均一测定
公开(公告)号：US06265170B1
公开(公告)日：2001-07-24
申请号：US09490273
申请日：2000-01-24
申请人： Pierre Picard , Jasmine I. Daksis , Glen H. Erikson
发明人： Pierre Picard , Jasmine I. Daksis , Glen H. Erikson
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q2563/113 , Y10T436/143333 , C12Q2563/107 , C12Q2523/307 , C12Q2525/107
摘要： The invention provides homogeneous assay methods for nucleic acid hybridization, detection and evaluation. The assay includes obtaining signals from a test sample both before and during the application of a voltage to the test sample and correlating the signals, each of which is indicative of the binding affinity of the probe and the target to each other. The assay enables determining an extent of matching between the probe and the target, as the voltage can be calibrated so as to destabilize significantly any hybridization except perfectly complementary hybridization. The signals whose magnitude is correlated with binding affinity can be electrical conductance and/or fluorescent intensity. Preferably, both signal pairs are measured and compared so as to enhance the reliability of the assay. The assay can detect specific hybridization between single-stranded probes and non-denatured double-stranded targets to form triplexes, thus obviating the need to denature the targets. The assay methods can also be applied to duplex hybridization complexes.
摘要翻译：本发明提供用于核酸杂交，检测和评价的均一测定方法。该测定包括在测试样品施加电压之前和期间从测试样品获得信号，并将信号相关联，每个信号指示探针和靶标彼此的结合亲合力。该测定能够确定探针与靶标之间的匹配程度，因为电压可被校准，以便显着地使除了完全互补杂交之外的任何杂交不稳定。幅度与结合亲和力相关的信号可以是电导和/或荧光强度。优选地，测量和比较两个信号对，以增强测定的可靠性。该测定可以检测单链探针和非变性双链靶标之间的特异性杂交以形成三链体，从而避免了使靶变性的需要。测定方法也可应用于双链杂交复合物。

5. 发明授权

US07309569B2 Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes 有权
标题翻译：核酸或其类似物的平行或反向平行，同源或互补结合形成双链体，三链体或四链体复合物
公开(公告)号：US07309569B2
公开(公告)日：2007-12-18
申请号：US10293148
申请日：2002-11-12
申请人： Glen H. Erikson , Jasmine I. Daksis , Ivana Kandic , Pierre Picard
发明人： Glen H. Erikson , Jasmine I. Daksis , Ivana Kandic , Pierre Picard
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6839 , C12Q1/6816 , C12Q1/6827 , C12Q2525/113 , C12Q2525/101 , C12Q2563/173 , C12Q2563/107 , C12Q2537/119 , C12Q2527/125 , C12Q2563/137
摘要： A complex includes: (1) a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues; and (2) a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues, wherein: (a) at least one of the probe and the target is purified or synthetic; and (b) the heteropolymeric probe sequence is bonded to the heteropolymeric target sequence by Watson-Crick complementary base interaction or by homologous base interaction, provided that when the complex is a duplex and the heteropolymeric probe sequence is antiparallel to the heteropolymeric target sequence, the heteropolymeric probe sequence is bonded to the heteropolymeric target sequence by homologous base interaction, and provided that when the complex is a triplex, the complex is free of recombination proteins. A method for assaying a target includes detecting formation of the complex.
摘要翻译：复合物包括：（1）含有核酸或核酸类似物的异源聚合物探针序列的探针; 和（2）含有核酸或核酸类似物的异源聚合物靶序列的靶，其中：（a）所述探针和靶中的至少一个被纯化或合成; 并且（b）异源聚合物探针序列通过Watson-Crick互补碱基相互作用或通过同源碱基相互作用结合到异源聚合物靶序列，条件是当复合物是双链体并且异源聚合物探针序列与异源聚合物靶序列反平行时，异源聚合物探针序列通过同源碱基相互作用结合到异源聚合物靶序列，并且条件是当复合物是三链体时，复合物不含重组蛋白质。用于测定靶的方法包括检测复合物的形成。

6. 发明授权

US07220541B2 Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions 失效
标题翻译：通过在不同条件下进行多次测量，生物聚合物结合的均相测定
公开(公告)号：US07220541B2
公开(公告)日：2007-05-22
申请号：US09911047
申请日：2001-07-23
申请人： Glen H. Erikson , Jasmine I. Daksis , Pierre Picard
发明人： Glen H. Erikson , Jasmine I. Daksis , Pierre Picard
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34 , B32B5/02
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q2563/113 , C12Q2563/107 , C12Q2523/307 , C12Q2525/107
摘要： A method for homogeneously assaying biopolymer bonding includes obtaining signals from a test sample before, during and/or after the application of stimulus to the test sample and correlating the signals. The signals, whose magnitude correlate with binding affinity, can be, for example, electrical conductance and/or fluorescent intensity. The stimulus can be, for example, electric voltage and/or laser radiation. Preferably, different types of signals are measured and compared so as to enhance the reliability of the assay.
摘要翻译：用于均匀测定生物聚合物键合的方法包括在向测试样品施加刺激之前，期间和/或之后从测试样品获得信号并使信号相关。与结合亲和力相关的幅度的信号可以是例如电导和/或荧光强度。刺激可以是例如电压和/或激光辐射。优选地，测量和比较不同类型的信号，以增强测定的可靠性。

7. 发明授权

US06878815B2 Heteropolymeric triplexes containing watson-crick triplets 失效
标题翻译：含有watson-crick三联体的异种复合物
公开(公告)号：US06878815B2
公开(公告)日：2005-04-12
申请号：US10103002
申请日：2002-03-21
申请人： Glen H. Erikson , Jasmine I. Daksis , Pierre Picard
发明人： Glen H. Erikson , Jasmine I. Daksis , Pierre Picard
IPC分类号： G01N21/64 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/483 , G01N33/53 , G01N33/542 , G01N33/566 , G01N33/58 , G01N37/00 , C07H21/04
CPC分类号： C12Q1/6839 , C12Q1/6816 , C12Q1/6827 , C12Q2563/137 , C12Q2563/107 , C12Q2563/173
摘要： Triplex complexes contain a single-stranded probe bound to a double-stranded nucleic acid target, in which the probe includes a heteropolymeric nucleic acid or a heteropolymeric nucleic acid analog. All base triplets of the complex are members selected from the group consisting of A-T-A, T-A-T, U-A-T, T-A-U, A-U-A, U-A-U, G-C-G and C-G-C. A cation-facilitated assay includes detecting the presence of such triplex complexes to determine the degree of complementarity between the probe and target sequence. The assay preferably detects a change in fluorescent intensity of a label as a function of binding affinity between the probe and target. The label can be covalently tethered to the probe or to the target, or can be an intercalating fluorophore in the reaction medium.
摘要翻译：三重配合物含有与双链核酸靶结合的单链探针，其中探针包括杂聚核酸或异源核酸类似物。复合体的所有基本三元组是从由A-T-A，T-A-T，U-A-T，T-A-U，A-U-A，U-A-U，G-C-G和C-G-C组成的组中选出的成员。阳离子促进测定法包括检测这种三重配合物的存在以确定探针和靶序列之间的互补程度。该测定优选检测作为探针和靶标之间的结合亲合力的函数的标记物的荧光强度的变化。标记可以共价连接到探针或靶上，或者可以是反应介质中的插层荧光团。

8. 发明授权

US06656692B2 Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes 有权
公开(公告)号：US06656692B2
公开(公告)日：2003-12-02
申请号：US09909496
申请日：2001-07-20
申请人： Glen H. Erikson , Jasmine I. Daksis , Ivana Kandic , Pierre Picard
发明人： Glen H. Erikson , Jasmine I. Daksis , Ivana Kandic , Pierre Picard
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6816 , C12Q1/6839 , C12Q2563/173 , C12Q2563/107 , C12Q2537/119 , C12Q2527/125 , C12Q2563/137
摘要： A complex includes: (1) a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues; and (2) a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues, wherein: (a) at least one of the probe and the target is purified or synthetic; and (b) the heteropolymeric probe sequence is bonded to the heteropolymeric target sequence by Watson-Crick complementary base interaction or by homologous base interaction, provided that when the complex is a duplex and the heteropolymeric probe sequence is antiparallel to the heteropolymeric target sequence, the heteropolymeric probe sequence is bonded to the heteropolymeric target sequence by homologous base interaction, and provided that when the complex is a triplex, the complex is free of recombination proteins. A method for assaying a target includes detecting formation of the complex.

9. 发明授权

US06403313B1 Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators 有权
标题翻译：使用荧光嵌入剂的双链体和三重核酸杂交溶液的荧光强度测定
公开(公告)号：US06403313B1
公开(公告)日：2002-06-11
申请号：US09468679
申请日：1999-12-21
申请人： Jasmine I. Daksis , Pierre Picard , Glen H. Erikson
发明人： Jasmine I. Daksis , Pierre Picard , Glen H. Erikson
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6839 , C12Q2563/173 , C12Q2563/107
摘要： The invention provides a homogeneous assay for nucleic acid hybridization. The fluorescent intensity of a hybridization medium containing a probe, a target and an intercalating agent is a function of the hybridization efficiency of the probe with respect to the target. The assay can detect specific hybridization between single-stranded probes and non-denatured double-stranded targets to form triplexes, thus obviating the need to denature the targets. The assay can also detect duplex hybridization complexes. The assay can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.
摘要翻译：本发明提供了用于核酸杂交的均一测定。包含探针，靶和插入剂的杂交介质的荧光强度是探针相对于靶的杂交效率的函数。该测定可以检测单链探针和非变性双链靶标之间的特异性杂交以形成三链体，从而避免了使靶变性的需要。该测定还可以检测双链杂交复合物。该测定可用于鉴定折叠的核苷酸序列中的可接近区域，以确定杂交复合物中错配对的数目，并绘制基因组。

10. 发明授权

US07321116B2 Ionization source for mass spectrometer 有权
标题翻译：质谱仪电离源
公开(公告)号：US07321116B2
公开(公告)日：2008-01-22
申请号：US11133896
申请日：2005-05-20
申请人： Pierre Picard , Denis Lessard , André L'Heureux , Jean Lacoursiere , Philippe Nobert , Sylvain Letarte , Alexandre Vallieres , Robert Tiveron , Réal Paquin
发明人： Pierre Picard , Denis Lessard , André L'Heureux , Jean Lacoursiere , Philippe Nobert , Sylvain Letarte , Alexandre Vallieres , Robert Tiveron , Réal Paquin
IPC分类号： H01J49/04
CPC分类号： H01J49/0463 , H01J49/049 , H01J49/168
摘要： An apparatus and method for regenerating ion samples for a mass spectrometer are provided. Source samples are loaded on a support which is heated by a laser beam, desorbing the sample without ionization. The desorbed sample is carried by a carrier gas flow through a transfer tube, at the output of which it is ionized by corona discharge or photo-ionization. The obtained ionized sample may be analyzed in a mass spectrometer or used to serve any other appropriate purpose.
摘要翻译：提供了用于质谱仪再生离子样品的装置和方法。源样品被加载在被激光束加热的支撑件上，而不离子地解吸样品。解吸的样品由载气流通过传输管承载，其输出通过电晕放电或光电离被离子化。所获得的电离样品可以在质谱仪中进行分析或者用于任何其它合适的目的。

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