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1. 发明专利

CA2239988A1 ELECTROPHORESIS GELS AND GEL HOLDERS HAVING FIBER SPACERS AND METHOD OF MAKING SAME 未知
公开(公告)号：CA2239988A1
公开(公告)日：1997-06-19
申请号：CA2239988
申请日：1996-12-12
申请人： WATERHOUSE PAUL , IZMAILOV ALEXANDRE , VISIBLE GENETICS INC , ZALESKI HENRYK
发明人： WATERHOUSE PAUL , IZMAILOV ALEXANDRE , ZALESKI HENRYK
IPC分类号： G01N21/64 , G01N27/447 , G01N27/453
摘要： Gel holders for electrophoresis gels are made using clad fibers, particularly glass fibers as spacers between substrates. A plurality of fibers with a highmelting interior core and a low-melting external cladding are placed between a first planar substrate and a second planar substrate. The fibers are heated to a temperatrure sufficient to at least soften the exterior cladding of the fibers without softening the interior core of the fibers, and then cooled while they are in contact with the first and second substrates to resolidify the exterior cladding. This adheres the fibers to the first and second substrates, and forms a gel chamber between said first and second substrates. The gel chamber has a thickness defined by interior core of the fibers. The fibers may be heated before or after the second substrate is placed over the top of the fibers. The gel holders thus formed may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely and used as needed.

2. 发明专利

CA2239982A1 ELECTROPHORESIS GELS AND GEL HOLDERS HAVING ADHESIVE AFFIXED FIBER SPACERS AND METHOD OF MAKING SAME 未知
公开(公告)号：CA2239982A1
公开(公告)日：1997-06-19
申请号：CA2239982
申请日：1996-12-12
申请人： IZMAILOV ALEXANDRE , VISIBLE GENETICS INC , WATERHOUSE PAUL , ZALESKI HENRYK
发明人： IZMAILOV ALEXANDRE , WATERHOUSE PAUL , ZALESKI HENRYK
IPC分类号： G01N27/447 , G01N27/453
摘要： Gel holders for electrophoresis gels are made using fibers, particularly glass fibers, which are affixed to the substrates forming the gel holder using an adhesive. These gel holders can be made by placing a plurality of adhesivecoated fibers between a first planar substrate and a second planar substrate; and applying pressure to the outside of the substrates to adhere the fibers to the first and second substrates. This forms a gel chamber between the first and second substrates which has a thickness defined by diameter of the fibers. Alternatively, uncoated fibers may be laid down in pairs, with a line of adhesive disposed between each fiber of the pair. When the adhesive is cured, it binds the fibers in position as spacers. At the same time, the fibers isolate the adhesive from the gel compartment. In this way, interference of components of the adhesive with the polymerization of the gel in the gel chamber can be avoided. Gel holders formed using either of these methods may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely and used as needed.

3. 发明专利

CA2207488A1 AUTOMATED ELECTROPHORESIS AND FLUORESCENCE DETECTION APPARATUS AND METHOD 未知
公开(公告)号：CA2207488A1
公开(公告)日：1996-06-20
申请号：CA2207488
申请日：1995-12-12
申请人： WATERHOUSE PAUL , VISIBLE GENETICS INC , STEVENS JOHN K , RENFREW JOHN A
发明人： WATERHOUSE PAUL , STEVENS JOHN K , RENFREW JOHN A
IPC分类号： G01N21/64 , G01N27/447
摘要： Improved detection methods and apparatus which may be used individually or in various combinations enhance the ability of the electrophoresis apparatus to detect fluorophore-labeled materials in short periods of time. One such apparatus comprises a housing adapted to receive an electrophoresis gel holder; an excitation source of electromagnetic radiation having a frequency effective to induce emission of electromagnetic radiation from the fluorophore; a plurality of optical fibers for delivering electromagnetic radiation from the excitation source to a linear array of excitation/detection sites on the gel holder, optical switching means for sequentially directing electromagnetic radiation into one of several pre-defined groups of the optical fibers; detection means such as a photomultiplier tube, or an array of photomultiplier tubes for detecting emission from the fluorophore induced by a radiation from the excitation source; and means for correlating a detected emission with the switching of the excitation electromagnetic radiation such that a given emission may be linked with the excitation/detection site being irradiated. For example, the optical switching means may alternate between directing radiation from the source into every other optical fiber, or may provide radiation in rotation to every third or fourth fiber. Alternatively, a spot array generation grating can be used for dividing an incident beam of coherent radiation into an array of excitation beamlets and directing each excitation beamlet to an excitation/detection site on the electrophoresis gel. Light emitting diode disposed to deliver excitation energy to an array of excitation/detection sites may also be used. This latter form of the apparatus is particularly advantageous due to the low costs of light emitting diodes (LEDs) compared to coherent light sources (e.g. lasers).

4. 发明专利

DE69530856T2 未知
公开(公告)号：DE69530856T2
公开(公告)日：2004-03-11
申请号：DE69530856
申请日：1995-10-31
申请人： VISIBLE GENETICS INC
发明人： RENFREW A , WATERHOUSE PAUL , STEVENS K , ZALESKI HENRYK
IPC分类号： G01N27/447
摘要： A loading insert for use in loading a sample onto an electrophoresis gel, comprising a substantially rectangular planar base portion (61), a plurality of fingers (62) extending from a first long edge of the base portion in a plane with the upper and lower faces of the base portion, each of said fingers having a defined width and being evenly spaced. Each of said fingers having a tip portion being cut with a 30-60 DEG bevel relative to the plane of the base portion. A filling insert for use in filling an electrophoresis gel holder comprising a substantially rectangular planar body member having a first of its long edges beveled at an angle of from 30 to 60 degrees relative to the plane of the body member, and a plurality of grooves (101) formed in its lower surface, said grooves running parallel to the short edges and extending from the one long edge to the other.

5. 发明专利

CA2222628A1 MICROELECTROPHORESIS CHIP FOR MOVING AND SEPARATING NUCLEIC ACIDS AND OTHER CHARGED MOLECULES 未知
公开(公告)号：CA2222628A1
公开(公告)日：1996-12-27
申请号：CA2222628
申请日：1996-06-07
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , IZMAILOV ALEXANDRE M , MARUZZO BRUNO , WATERHOUSE PAUL , LARSON MARINA T , STEVENS JOHN K
IPC分类号： C12N15/09 , B82B1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/58 , G01N1/00
摘要： A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 .mu.m wide and 1 to 10 .mu.m in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric field within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

6. 发明专利

AU702083B2 Microelectrophoresis chip for moving and separating nucleic acids and other charged molecules 未知
公开(公告)号：AU702083B2
公开(公告)日：1999-02-11
申请号：AU6274696
申请日：1996-06-07
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , WATERHOUSE PAUL , IZMAILOV ALEXANDRE M , MARUZZO BRUNO , STEVENS JOHN K , LARSON MARINA T
IPC分类号： C12N15/09 , B82B1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/58
摘要： A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 mum wide and 1 to 10 mum in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric filed within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

7. 发明专利

AU5947498A Microgels for use in medical diagnosis and methods of making and using same 未知
公开(公告)号：AU5947498A
公开(公告)日：1998-06-04
申请号：AU5947498
申请日：1998-03-24
申请人： VISIBLE GENETICS INC
发明人： RENFREW JOHN A , WATERHOUSE PAUL , STEVENS JOHN K , ZALESKI HENRYK
IPC分类号： G01N27/447 , G01N27/26
摘要： A loading insert for use in loading a sample onto an electrophoresis gel, comprising a substantially rectangular planar base portion (61), a plurality of fingers (62) extending from a first long edge of the base portion in a plane with the upper and lower faces of the base portion, each of said fingers having a defined width and being evenly spaced. Each of said fingers having a tip portion being cut with a 30-60 DEG bevel relative to the plane of the base portion. A filling insert for use in filling an electrophoresis gel holder comprising a substantially rectangular planar body member having a first of its long edges beveled at an angle of from 30 to 60 degrees relative to the plane of the body member, and a plurality of grooves (101) formed in its lower surface, said grooves running parallel to the short edges and extending from the one long edge to the other.

8. 发明专利

CA2264961A1 APPARATUS AND METHOD FOR PERFORMING SEQUENCING OF NUCLEIC ACID POLYMERS 未知
公开(公告)号：CA2264961A1
公开(公告)日：1998-03-05
申请号：CA2264961
申请日：1997-08-27
申请人： VISIBLE GENETICS INC
发明人： DUNN JAMES M , HUI MAY , IZMAILOV ALEXANDRE M , WATERHOUSE PAUL , LEUSHNER JAMES , ZALESKI HENRYK , YAGER THOMAS D , LARSON MARINA T
IPC分类号： B01L7/00 , G01N27/447 , C12Q1/68
摘要： An apparatus for processing samples containing DNA to produce a sequencing fragment mixture comprises a sample processing element comprising: a thermocycling region having one or more chambers for receiving a DNA sequencing reaction mixture and forming sequencing fragments therefrom; a separation region comprising a separation matrix for separating the sequencing fragments formed in the thermocycling regions; a detection region for detection of the separated sequencing fragments; and means for regulating the temperature in the thermocycling region of the sample processing element to provide a plurality of thermal cycles, each cycle including at least a denaturation phase and an extension phase. The apparatus for processing sample can be placed in a holder which is associated with means for applying an electric field to the separation region of a sample processing apparatus placed within the holder to cause polynucleotide sequencing fragments to migrate through the separation region from the thermocycling region to the detection region; and means for detecting polynucleotide fragments within the detection region of the sample processing apparatus placed within the holder.

9. 发明专利

AU2378097A Method and apparatus for thermal cycling and for automated sample preparation with thermal cycling 未知
公开(公告)号：AU2378097A
公开(公告)日：1997-11-19
申请号：AU2378097
申请日：1997-05-01
申请人： VISIBLE GENETICS INC
发明人： DUNN JAMES M , LEUSHNER JAMES , RENFREW JOHN A , WATERHOUSE PAUL , IZMAILOV ALEXANDRE M , ZALESKI HENRYK
IPC分类号： B01L7/00 , C12Q1/68 , C12Q1/70 , G01N35/00

10. 发明专利

AU705355B2 Electrophoresis gels and gel holders having adhesive affixed fiber spacers and method of making same 未知
公开(公告)号：AU705355B2
公开(公告)日：1999-05-20
申请号：AU1027597
申请日：1996-12-12
申请人： VISIBLE GENETICS INC
发明人： IZMAILOV ALEXANDRE , ZALESKI HENRYK , WATERHOUSE PAUL
IPC分类号： G01N27/447
摘要： Gel holders for electrophoresis gels are made using fibers, particularly glass fibers, which are affixed to the substrates forming the gel holder using an adhesive. These gel holders can be made by placing a plurality of adhesive-coated fibers between a first planar substrate and a second planar substrate; and applying pressure to the outside of the substrates to adhere the fibers to the first and second substrates. This forms a gel chamber between the first and second substrates which has a thickness defined by diameter of the fibers. Alternatively, uncoated fibers may be laid down in pairs, with a line of adhesive disposed between each fiber of the pair. When the adhesive is cured, it binds the fibers in position as spacers. At the same time, the fibers isolate the adhesive from the gel compartment. In this way, interference of components of the adhesive with the polymerization of the a gel in the gel chamber can be avoided. Gel holders formed using either of these methods may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely and used as needed.

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