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1. 发明专利

CA2222628A1 MICROELECTROPHORESIS CHIP FOR MOVING AND SEPARATING NUCLEIC ACIDS AND OTHER CHARGED MOLECULES 未知
公开(公告)号：CA2222628A1
公开(公告)日：1996-12-27
申请号：CA2222628
申请日：1996-06-07
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , IZMAILOV ALEXANDRE M , MARUZZO BRUNO , WATERHOUSE PAUL , LARSON MARINA T , STEVENS JOHN K
IPC分类号： C12N15/09 , B82B1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/58 , G01N1/00
摘要： A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 .mu.m wide and 1 to 10 .mu.m in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric field within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

2. 发明专利

CA2226405A1 NANOFABRICATED SEPARATION MATRIX FOR ANALYSIS OF BIOPOLYMERS AND METHODS OF MAKING AND USING SAME 未知
公开(公告)号：CA2226405A1
公开(公告)日：1996-12-27
申请号：CA2226405
申请日：1996-06-07
申请人： VISIBLE GENETICS INC
发明人： LARSON MARINA T , STEVENS JOHN K , MARUZZO BRUNO , WATERHOUSE PAUL , IZMAILOV ALEXANDRE M , YAGER THOMAS D
IPC分类号： C12N15/09 , B82B1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/58 , G01N1/34 , B01D57/02
摘要： Separation matrices useful in the formation of solid-state mm- to cm-scale devices for the rapid, high-resolution separation of single-stranded DNA ladder bands generated by the Sanger dideoxy- or Maxam/Gilbert chemical DNA sequencing procedures are formed from a solid support (1) having a plurality of posts (4) disposed on a first major surface thereof to form an obstacle course of posts (4) and pores (5). The posts are arranged in a regular X, Y array and are separated one from another by a distance of 100 nm or less, preferably 10 to 30 nm, and are optionally separated into lanes 2. The separation matrix can be manufactured by first forming a mold, preferably a reusable mold using lithography techniques. The mold is the reverse of the desired pattern of posts and pores of the obstacle course, and is used for casting the obstacle course. The cast obstacle course is then fused to a solid support and separated from the mold. Alternatively, the separation matrix can be formed from a polymer which undergoes specific and quantifiable swelling in the presence of a selected chemical compound. In this case, the matrix is cast on a mold in a conventional manner with a spacing between posts greater than the desired final spacing of 100 nm or less. For use, a buffer solution saturated with the specific chemical agent that controls swelling is added, causing the posts to swell to a defined amount to achieve the desired separation.

3. 发明专利

AU698921B2 Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same 未知
公开(公告)号：AU698921B2
公开(公告)日：1998-11-12
申请号：AU6271896
申请日：1996-06-07
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , WATERHOUSE PAUL , IZMAILOV ALEXANDRE M , MARUZZO BRUNO , STEVENS JOHN K , LARSON MARINA T
IPC分类号： C12N15/09 , B82B1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/58
摘要： A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 mum wide and 1 to 10 mum in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric filed within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

4. 发明专利

CA2227611A1 METHOD FOR IDENTIFICATION OF MUTATIONS USING LIGATION OF MULTIPLE OLIGONUCLEOTIDE PROBES 未知
公开(公告)号：CA2227611A1
公开(公告)日：1997-03-06
申请号：CA2227611
申请日：1996-08-28
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , DUNN JAMES M
IPC分类号： C12N15/09 , C12Q1/68
摘要： A ligase-based assay which relies only upon knowledge of the wild-type sequence of a gene or gene fragment is used to detect all types of mutations, i.e., point mutations, insertions and deletions. The assay makes use of a set of oligonucleotide probes, which may be packaged in kit form, which hybridize in series along the length of the gene. The ligation of the probes together form a ligation product, the size of which is evaluated. When the gene or gene fragment being analyzed corresponds to the normal sequence and thus perfectly matches the probes, all of the probes in the set are ligated together, and the ligation product has a certain resulting size. When a mutation appears in the gene, the hybridization of the probe overlapping the mutation is impaired, with the result that some or all of the ligation product is of smaller size. By evaluating the size of the ligation product, both the existence of a mutation and its approximate position can be identified.

5. 发明申请

WO9708344A2 METHOD FOR IDENTIFICATION OF MUTATIONS USING LIGATION OF MULTIPLE OLIGONUCLEOTIDE PROBES 审中-公开
标题翻译：使用多个寡核苷酸探针识别突变体的方法
公开(公告)号：WO9708344A2
公开(公告)日：1997-03-06
申请号：PCT/US9614020
申请日：1996-08-28
申请人： VISIBLE GENETICS INC , YAGER THOMAS D , DUNN JAMES M
发明人： YAGER THOMAS D , DUNN JAMES M
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2563/107 , C12Q2533/107 , C12Q2527/107
摘要： A ligase-based assay which relies only upon knowledge of the wild-type sequence of a gene or gene fragment is used to detect all types of mutations, i.e., point mutations, insertions and deletions. The assay makes use of a set of oligonucleotide probes, which may be packaged in kit form, which hybridize in series along the length of the gene. The ligation of the probes together form a ligation product, the size of which is evaluated. When the gene or gene fragment being analyzed corresponds to the normal sequence and thus perfectly matches the probes, all of the probes in the set are ligated together, and the ligation product has a certain resulting size. When a mutation appears in the gene, the hybridization of the probe overlapping the mutation is impaired, with the result that some or all of the ligation product is of smaller size. By evaluating the size of the ligation product, both the existence of a mutation and its approximate position can be identified.
摘要翻译：仅依赖于基因或基因片段的野生型序列的知识的基于连接酶的测定法用于检测所有类型的突变，即点突变，插入和缺失。该测定使用一组寡核苷酸探针，其可以以试剂盒形式包装，其沿着该基因的长度串联杂交。探针的连接形成连接产物，评价其尺寸。当分析的基因或基因片段对应于正常序列，从而完美匹配探针时，将所有组中的所有探针连接在一起，并且连接产物具有一定的最终尺寸。当基因中出现突变时，与突变重叠的探针的杂交受损，结果是部分或全部连接产物的尺寸较小。通过评估连接产物的大小，可以确定突变的存在及其近似位置。

6. 发明专利

AU4836500A Nucleic acid sequencing with simultaneous quantitation 未知
公开(公告)号：AU4836500A
公开(公告)日：2000-11-21
申请号：AU4836500
申请日：2000-05-08
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , AUGUST M JASON
IPC分类号： C12Q1/68 , C12Q1/6851 , C12Q1/6869 , C12P19/34 , C12M1/34
摘要： Simultaneous sequencing and quantitation of a nucleic acid analyte in a sample using the same reagents for both assays is achieved by processing a sample containing, or suspected of containing the nucleic acid analyte of interest using a single set of reagents through a plurality of thermocycles to obtain a mixture of labeled polynucleotides which are used for the determination of both sequence information about the target nucleic acid and the amount of target nucleic acid present in the sample. The fragments are separated on the basis of size, for example by electrophoresis, and the label associated with the separated fragments is detected. The positions of the separated nucleic acid fragments are evaluated to obtain information about the sequence of the target nucleic acid analyte, and the intensity of a signal derived from the label associated with one or more of the separated fragments is evaluated to determine the quantity of the target nucleic acid analyte in the sample. Only one label is needed for both sequencing and quantitation, although two or more labels may be used if bidirectional sequencing is concurrently performed.

7. 发明专利

CA2373044A1 NUCLEIC ACID SEQUENCING WITH SIMULTANEOUS QUANTITATION 未知
公开(公告)号：CA2373044A1
公开(公告)日：2000-11-16
申请号：CA2373044
申请日：2000-05-08
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , AUGUST M JASON
IPC分类号： C12Q1/68 , C12Q1/6851 , C12Q1/6869 , C12P19/34 , C12M1/34
摘要： Simultaneous sequencing and quantitation of a nucleic acid analyte in a samp le using the same reagents for both assays is achieved by processing a sample containing, or suspected of containing the nucleic acid analyte of interest using a single set of reagents through a plurality of thermocycles to obtain a mixture of labeled polynucleotides which are used for the determination of both sequence information about the target nucleic acid and the amount of target nucleic acid present in the sample. The fragments are separated on th e basis of size, for example by electrophoresis, and the label associated with the separated fragments is detected. The positions of the separated nucleic acid fragments are evaluated to obtain information about the sequence of the target nucleic acid analyte, and the intensity of a signal derived from the label associated with one or more of the separated fragments is evaluated to determine the quantity of the target nucleic acid analyte in the sample. Onl y one label is needed for both sequencing and quantitation, although two or mo re labels may be used if bidirectional sequencing is concurrently performed.

8. 发明专利

AU4236597A Apparatus and method for performing sequencing of nucleic acid polymers 未知
公开(公告)号：AU4236597A
公开(公告)日：1998-03-19
申请号：AU4236597
申请日：1997-08-27
申请人： VISIBLE GENETICS INC
发明人： WATERHOUSE PAUL , IZMAILOV ALEXANDRE M , ZALESKI HENRYK , YAGER THOMAS D , DUNN JAMES M , LEUSHNER JAMES , HUI MAY , LARSON MARINA T
IPC分类号： B01L7/00 , G01N27/447 , C12Q1/68

9. 发明专利

AU704962B2 Method for identification of mutations using ligation of multiple oligonucleotide probes 未知
公开(公告)号：AU704962B2
公开(公告)日：1999-05-13
申请号：AU6908996
申请日：1996-08-28
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , DUNN JAMES M
IPC分类号： C12N15/09 , C12Q1/68 , C07H21/04
摘要： PCT No. PCT/US96/14020 Sec. 371 Date Feb. 23, 1998 Sec. 102(e) Date Feb. 23, 1998 PCT Filed Aug. 28, 1996 PCT Pub. No. WO97/08344 PCT Pub. Date Mar. 6, 1997A ligase-based assay which relies only upon knowledge of the wild-type sequence of a gene or gene fragment is used to detect all types of mutations, i.e., point mutations, insertions and deletions. The assay makes use of a set of oligonucleotide probes, which may be packaged in kit form, which hybridize in series along the length of the gene. The ligation of the probes together form a ligation product, the size of which is evaluated. When the gene or gene fragment being analyzed corresponds to the normal sequence and thus perfectly matches the probes, all of the probes in the set are ligated together, and the ligation produce has a certain resulting size. When a mutation appears in the gene, the hybridization of the probe overlapping the mutation is impaired, with the result that some or all of the ligation produce is of smaller size. By evaluating the size of the ligation product, both the existence of a mutation and its approx. position can be identified.

10. 发明专利

AU702083B2 Microelectrophoresis chip for moving and separating nucleic acids and other charged molecules 未知
公开(公告)号：AU702083B2
公开(公告)日：1999-02-11
申请号：AU6274696
申请日：1996-06-07
申请人： VISIBLE GENETICS INC
发明人： YAGER THOMAS D , WATERHOUSE PAUL , IZMAILOV ALEXANDRE M , MARUZZO BRUNO , STEVENS JOHN K , LARSON MARINA T
IPC分类号： C12N15/09 , B82B1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/58
摘要： A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 mum wide and 1 to 10 mum in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric filed within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

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