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1. 发明申请

WO2007059473A2 METHODS FOR THE PRODUCTION AND PURIFICATION OF ADENOVIRAL VECTORS 审中-公开
标题翻译：用于生产和纯化腺病毒载体的方法
公开(公告)号：WO2007059473A2
公开(公告)日：2007-05-24
申请号：PCT/US2006/060847
申请日：2006-11-13
申请人： INTROGEN THERAPEUTICS, INC. , ZHANG, Shuyuan , PHAM, Hai , SONG, Ping , CLARKE, Peter
发明人： ZHANG, Shuyuan , PHAM, Hai , SONG, Ping , CLARKE, Peter
IPC分类号： C12N7/02 , C12N15/861 , A61K48/00
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351
摘要： The present invention relates to compositions comprising and methods for producing adenovirus compositions wherein host cells are grown in a bioreactor and purified by size partitioning purification to provide purified adenovirus compositions.
摘要翻译：本发明涉及包含其中宿主细胞在生物反应器中生长并通过大小分配纯化纯化以提供纯化的腺病毒组合物的腺病毒组合物的组合物和方法。

2. 发明申请

WO2007134325A2 METHODS AND COMPOSITIONS FOR PROTEIN PRODUCTION USING ADENOVIRAL VECTORS 审中-公开
标题翻译：使用腺病毒载体生产蛋白质的方法和组合物
公开(公告)号：WO2007134325A2
公开(公告)日：2007-11-22
申请号：PCT/US2007/068977
申请日：2007-05-15
申请人： INTROGEN THERAPEUTICS, INC. , ZHANG, Shuyuan , PHAM, Hai , SONG, Ping , ZHENG, Ming , CLARKE, Peter
发明人： ZHANG, Shuyuan , PHAM, Hai , SONG, Ping , ZHENG, Ming , CLARKE, Peter
IPC分类号： C12N15/09
CPC分类号： C12N15/86 , C12N2710/10343
摘要： The present invention provides methods and compositions for recombinant protein production through replication-defective adenoviral vector infection of non-trans- complementation cell lines. Thus, this invention describes methods of heterologous protein production without an accompanied production of adenoviral vectors.
摘要翻译：本发明提供了通过非反互补细胞系的复制缺陷型腺病毒载体感染重组蛋白质生产的方法和组合物。因此，本发明描述了不伴随腺病毒载体产生的异源蛋白质生产的方法。

3. 发明申请

WO2006052302A2 A NOVEL METHOD FOR THE PRODUCTION AND PURIFICATION OF ADENOVIRAL VECTORS 审中-公开
标题翻译：用于生产和纯化腺病毒载体的新方法
公开(公告)号：WO2006052302A2
公开(公告)日：2006-05-18
申请号：PCT/US2005/026178
申请日：2005-07-22
申请人： INTROGEN THERAPEUTICS INC. , PHAM, Hai , ZHANG, Shuyuan , CLARKE, Peter
发明人： PHAM, Hai , ZHANG, Shuyuan , CLARKE, Peter
IPC分类号： C12Q1/70
CPC分类号： C12N15/86 , A61K48/00 , C12N7/00 , C12N15/1017 , C12N2710/10343 , C12N2710/10351 , C12Q1/70
摘要： The present invention relates to improved methods for producing adenovirus compositions wherein host cells are grown in a bioreactor and purified by size partitioning purification to provide purified adenovirus compositions.
摘要翻译：本发明涉及用于产生腺病毒组合物的改进方法，其中宿主细胞在生物反应器中生长并通过大小分配纯化纯化以提供纯化的腺病毒组合物。

4. 发明申请

WO2004104190A2 METHODS AND COMPOSITIONS FOR THE PRODUCTION OF ADENOVIRAL VECTORS 审中-公开
标题翻译：用于生产腺病毒载体的方法和组合物
公开(公告)号：WO2004104190A2
公开(公告)日：2004-12-02
申请号：PCT/US2004/015009
申请日：2004-05-14
申请人： INTROGEN THERAPEUTICS, INC. , ZHANG, Shuyuan , PHAM, Hai
发明人： ZHANG, Shuyuan , PHAM, Hai
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351
摘要： The present invention addresses the need to improve the yield of adenovirus when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of infection temperatures lower than 37°C in a cell culture system results in improved yields of adenovirus. In addition, it has been demonstrated that when host cells are grow in a bioreactor, initiating adenovirus infection by diluting the host cells with fresh media and adenovirus results in improved yield of adenovirus. Methods of adenoviral production and purification using infection temperatures less than 37°C are disclosed. Methods of adenoviral production and purification wherein the host cells are grown in a bioreactor and adenovirus infection is initiated by diluting the host cells with fresh media and adenovirus are also disclosed.
摘要翻译：本发明解决了在细胞培养系统中生长时提高腺病毒产量的需要。特别地，已经证明对于腺病毒，在细胞培养系统中使用低于37℃的感染温度导致腺病毒的产量提高。此外，已经证明当宿主细胞在生物反应器中生长时，通过用新鲜培养基和腺病毒稀释宿主细胞来引发腺病毒感染导致腺病毒的产量提高。公开了使用感染温度低于37℃的腺病毒产生和纯化方法。还公开了通过用新鲜培养基和腺病毒稀释宿主细胞来引发宿主细胞在生物反应器中生长和腺病毒感染的腺病毒产生和纯化的方法。

5. 发明申请

WO2008109721A1 CHROMATOGRAPHIC METHODS FOR ASSESSING ADENOVIRUS PURITY 审中-公开
标题翻译：用于评估腺病毒纯度的色谱方法
公开(公告)号：WO2008109721A1
公开(公告)日：2008-09-12
申请号：PCT/US2008/055995
申请日：2008-03-06
申请人： INTROGEN THERAPEUTICS, INC. , ZHANG, Shuyuan , CLARKE, Peter
发明人： ZHANG, Shuyuan , CLARKE, Peter
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N2710/10051 , C12N2710/10343 , C12N2710/10351
摘要： Methods for determining the quantity, quality and purity of a previously purified virus sample are disclosed. Such methods, which include the use of high performance size exclusion chromatography to determine these attributes are also disclosed.
摘要翻译：公开了用于确定先前纯化的病毒样品的数量，质量和纯度的方法。还公开了包括使用高性能尺寸排阻色谱法来确定这些属性的这些方法。

6. 发明申请

WO2006079014A3 TOPICAL ADMINISTRATION PERMITTING PROLONGED EXPOSURE OF TARGET CELLS TO THERAPEUTIC AND PROPHYLACTIC NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2006079014A3
公开(公告)日：2006-07-27
申请号：PCT/US2006/002255
申请日：2006-01-20
申请人： INTROGEN THERAPEUTICS, INC. , CLARKE, Peter , CHADA, Sunil , MENANDER, Kerstin , SOBOL, Robert , ZHANG, Shuyuan
发明人： CLARKE, Peter , CHADA, Sunil , MENANDER, Kerstin , SOBOL, Robert , ZHANG, Shuyuan
IPC分类号： A61K48/00 , C12N15/88
摘要： Compositions and methods for preventing or inhibiting the growth of a hyperproliferative lesion in a subject that include a nucleic acid comprised in a solid or semi¬ solid formation or in a transdermal or transcutaneous delivery device are disclosed. Also disclosed are compositions of a nucleic acid capable of preventing or inhibiting the growth of a hyperproliferative lesion in a subject that include an adhesive. Compositions of a nucleic acid capable of preventing or inhibiting the growth of a hyperproliferative lesion in a subject that include a nucleic acid uptake enhancer are also disclosed. Methods of preventing or inhibiting the growth of a hyperproliferative lesion in a subject that involve these therapeutic compositions and devices are also disclosed.

7. 发明申请

WO2008106646A2 METHODS AND FORMULATIONS FOR TOPICAL GENE THERAPY 审中-公开
标题翻译：用于局部基因治疗的方法和制剂
公开(公告)号：WO2008106646A2
公开(公告)日：2008-09-04
申请号：PCT/US2008/055507
申请日：2008-02-29
申请人： INTROGEN THERAPEUTICS, INC , ZHANG, Shuyuan , ONISHI, Eric
发明人： ZHANG, Shuyuan , ONISHI, Eric
IPC分类号： A61K9/00 , A61K9/70 , A61K48/00 , C12N15/86
CPC分类号： A61K48/0075 , A61K9/006 , A61K9/19 , A61K9/7007 , A61K48/0008
摘要： Formulations of viral vectors for topical application are disclosed as well as methods for making the same. Also disclosed are methods of treating a subject or diagnosing disease in a subject using the formulations of the present invention.
摘要翻译：公开了用于局部应用的病毒载体的制剂及其制备方法。还公开了使用本发明的制剂治疗受试者或诊断受试者疾病的方法。

8. 发明授权

EP0968284B1 AN IMPROVED METHOD FOR THE PRODUCTION AND PURIFICATION OF ADENOVIRAL VECTORS 失效
标题翻译：一种改进的方法进行生产和腺病毒载体的清洁
公开(公告)号：EP0968284B1
公开(公告)日：2006-12-13
申请号：EP97950677.1
申请日：1997-11-20
申请人： Introgen Therapeutics, Inc.
发明人： ZHANG, Shuyuan , TWIN, Capucine , WU, Zheng , CHO, Toohyoon , CASTON, Lucetta , WILSON, Deborah
IPC分类号： C12N15/12 , C12N15/11 , C12N15/86 , C12N7/02 , C12N7/01 , C12N5/08
CPC分类号： C12N7/00 , A61K35/761 , A61K38/00 , A61K48/00 , C07K14/4746 , C12N15/86 , C12N2710/10045 , C12N2710/10051 , C12N2710/10322 , C12N2710/10332 , C12N2710/10343 , C12N2710/10351 , C12N2810/6018
摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

9. 发明公开

EP1135469A1 AN IMPROVED METHOD FOR THE PRODUCTION AND PURIFICATION OF ADENOVIRAL VECTORS 无效
标题翻译：改进方法用于生产和腺病毒载体的清洁
公开(公告)号：EP1135469A1
公开(公告)日：2001-09-26
申请号：EP99960336.8
申请日：1999-11-16
申请人： Introgen Therapeutics, Inc.
发明人： ZHANG, Shuyuan , THWIN, Capucine , WU, Zheng , CHO, Toohyon , GALLAGHER, Shawn
IPC分类号： C12N7/02 , C12N7/01
CPC分类号： C07K14/4746 , A61K35/761 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351 , C12N2710/10352 , C12N2750/14143
摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

10. 发明公开

EP1815029A2 A NOVEL METHOD FOR THE PRODUCTION AND PURIFICATION OF ADENOVIRAL VECTORS 审中-公开
标题翻译：新方法的腺病毒载体的产生和清洁
公开(公告)号：EP1815029A2
公开(公告)日：2007-08-08
申请号：EP05781055.8
申请日：2005-07-22
申请人： Introgen Therapeutics, Inc.
发明人： PHAM, Hai , ZHANG, Shuyuan , CLARKE, Peter
IPC分类号： C12Q1/70
CPC分类号： C12N15/86 , A61K48/00 , C12N7/00 , C12N15/1017 , C12N2710/10343 , C12N2710/10351 , C12Q1/70
摘要： The present invention relates to improved methods for producing adenovirus compositions wherein host cells are grown in a bioreactor and purified by size partitioning purification to provide purified adenovirus compositions.

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