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    • 3. 发明授权
    • Method of isolating and culturing mesenchymal stem cell derived from umbilical cord blood
    • 分离和培养衍生自脐带血的间充质干细胞的方法
    • US07704739B2
    • 2010-04-27
    • US10579070
    • 2004-10-25
    • Hoon HanSung-Whan Kim
    • Hoon HanSung-Whan Kim
    • C12N5/00C12N5/08
    • C12N5/0665C12N2501/125C12N2501/22C12N2501/2303C12N2501/2306
    • The present invention relates to a method of isolating and culturing mesenchymal stem cells using umbilical cord blood that is most ideal for cell therapy. The method comprises adding an anti-coagulant to umbilical cord blood having a volume of more than 45 ml per unit, which is obtained within 24 hours after parturition; diluting the resulting mixture of the anti-coagulant and umbilical cord blood with an αMEM (alpha-minimum essential medium), followed by centrifugation to harvest monocytes; and subjecting the obtained monocytes into suspension culture in the αMEM containing Stem Cell Factor, GM-CSF (granulocyte-macrophage colony-stimulating factor), G-CSF (granulocyte colony-stimulating factor), IL-3 (interleukin-3) and IL-6 (interleukin-6).
    • 本发明涉及一种使用脐带血分离和培养间充质干细胞的方法,其是细胞治疗最理想的方法。 该方法包括向分娩后24小时内获得的每单位体积超过45ml的脐带血添加抗凝血剂; 用αMEM(α-minimum必需培养基)稀释所得的抗凝血剂和脐带血混合物,然后离心收获单核细胞; 将所获得的单核细胞在含有干细胞因子,GM-CSF(粒细胞 - 巨噬细胞集落刺激因子),G-CSF(粒细胞集落刺激因子),IL-3(白细胞介素-3)和IL -6(白介素-6)。