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1. 发明授权

US4234691A L-Lysine .alpha.-oxidase 失效
标题翻译： L-赖氨酸α-氧化酶
公开(公告)号：US4234691A
公开(公告)日：1980-11-18
申请号：US15179
申请日：1979-02-26
申请人： Hitoshi Kusakabe , Kenjiro Kodama , Yuichiro Midorikawa , Akira Kuninaka , Haruo Misono , Kenji Soda
发明人： Hitoshi Kusakabe , Kenjiro Kodama , Yuichiro Midorikawa , Akira Kuninaka , Haruo Misono , Kenji Soda
IPC分类号： A61K38/44 , C12N9/06 , C12Q1/26
CPC分类号： C12N9/0022 , C12Q1/26 , C12Y104/03014 , A61K38/00 , Y10S435/945
摘要： An L-lysine .alpha.-oxidase, that is, a novel L-amino acid oxidase having very high substrate-specificity to L-lysine is produced by culturing a specific microorganism belonging to Trichoderma in a medium.
摘要翻译：通过在培养基中培养属于木霉属的特定微生物，产生L-赖氨酸α-氧化酶，即对L-赖氨酸具有非常高的底物特异性的新的L-氨基酸氧化酶。

2. 发明授权

US4224407A Assay of L-lysine 失效
标题翻译： L-赖氨酸的测定
公开(公告)号：US4224407A
公开(公告)日：1980-09-23
申请号：US15177
申请日：1979-02-26
申请人： Hitoshi Kusakabe , Akira Kuninaka , Kenji Soda
发明人： Hitoshi Kusakabe , Akira Kuninaka , Kenji Soda
IPC分类号： C12P13/00 , A61K38/44 , C12N9/06 , C12P17/12 , C12Q1/26 , C12Q1/28 , C12R1/885 , G01N33/02 , G01N33/68 , C07G7/02
CPC分类号： C12N9/0022 , C12Q1/26 , C12Y104/03014 , A61K38/00 , Y10S435/945
摘要： L-lysine contained in a sample can be efficiently determined by using the L-lysine .alpha.-oxidase. The preferred enzyme is an L-lysine .alpha.-oxidase, that is, a novel L-amino acid oxidase having very high substrate-specificity to L-lysine is produced by culturing a specific microorganism belonging to Trichoderma in a medium.
摘要翻译：通过使用L-赖氨酸α-氧化酶可以有效地测定样品中所含的L-赖氨酸。优选的酶是L-赖氨酸α-氧化酶，即通过在培养基中培养属于木霉属的特定微生物而产生对L-赖氨酸具有非常高的底物特异性的新型L-氨基酸氧化酶。

3. 发明授权

US07247466B2 Amino acid racemase having low substrate specificity and process for producing racemic amino acid 有权
标题翻译：具有低底物特异性的氨基酸消旋酶和用于生产外消旋氨基酸的方法
公开(公告)号：US07247466B2
公开(公告)日：2007-07-24
申请号：US10506152
申请日：2003-02-28
申请人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto , Makoto Yagasaki , Kenji Soda
发明人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto , Makoto Yagasaki , Kenji Soda
IPC分类号： C07H21/04 , C12N9/90 , C12N15/74 , C12N1/21 , C12P21/06
CPC分类号： C12P13/04 , C12N9/90
摘要： The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.
摘要翻译：本发明提供了具有低底物特异性氨基酸消旋酶活性的蛋白质; 编码蛋白质的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 通过使用转化体生产蛋白质的方法; 以及制备外消旋氨基酸的方法，其包括使转化体或其处理物质的培养物作为酶源，并且氨基酸存在于水性介质中以使水性介质中的氨基酸外消旋化，并回收来自水介质的外消旋氨基酸。

4. 发明授权

US4546077A Leucine dehydrogenase and a process for production thereof 失效
标题翻译：亮氨酸脱氢酶及其制备方法
公开(公告)号：US4546077A
公开(公告)日：1985-10-08
申请号：US525905
申请日：1983-08-24
申请人： Toshihisa Ohshima , Kenji Soda
发明人： Toshihisa Ohshima , Kenji Soda
IPC分类号： C12N9/06 , C12Q1/32 , C12Q1/37 , C12R1/07
CPC分类号： C12N9/0016 , C12Q1/32 , C12Q1/37 , Y10S435/832
摘要： Leucine dehydrogenase is disclosed which retains 80% or more of the activity even after it is incubated in a buffer solution at about 70.degree. C. for 10 minutes, as compared to the activity prior to incubation. A process for producing a heat resistant leucine dehydrogenase is also disclosed. The process comprises culturing a microorganism having an optimum growth temperature of 50.degree. to 85.degree. C. and harvesting the leucine dehydrogenase from the culture and a composition of assaying for leucine aminopeptidase (LAP) comprising the heat resistant leucine dehydrogenase.The leucine dehydrogenase can be stored for a long period of time and thus very effective for investigations in biochemistry, food analysis and clinical test. Further the composition provides extremely easy assay for the LAP activity in high accuracy with good reproducibility which is an important item in clinical test.
摘要翻译：与在孵育前的活性相比，公开了即使在与约70℃的缓冲溶液中孵育10分钟后也保持80％或更高活性的亮氨酸脱氢酶。还公开了一种生产耐热亮氨酸脱氢酶的方法。该方法包括培养具有最佳生长温度为50至85℃的微生物，并从培养物中收获亮氨酸脱氢酶，以及测定含有耐热亮氨酸脱氢酶的亮氨酸氨基肽酶（LAP）的组合物。亮氨酸脱氢酶可以长期储存，因此对于生物化学，食品分析和临床试验的研究非常有效。此外，该组合物以高精度提供对LAP活性的非常容易的测定，具有良好的再现性，这是临床测试中的重要项目。

5. 发明申请

US20090226999A1 Low-substrate-specific amino acid racemase and process for producing racemic amino acid 失效
标题翻译：低底物特异性氨基酸消旋酶及其制备外消旋氨基酸的方法
公开(公告)号：US20090226999A1
公开(公告)日：2009-09-10
申请号：US11808186
申请日：2007-06-07
申请人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto , Makoto Yagasaki , Kenji Soda
发明人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto , Makoto Yagasaki , Kenji Soda
IPC分类号： C12N9/90 , C12N15/61 , C12N5/10
CPC分类号： C12P13/04 , C12N9/90
摘要： The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.
摘要翻译：本发明提供了具有低底物特异性氨基酸消旋酶活性的蛋白质; 编码蛋白质的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 通过使用转化体生产蛋白质的方法; 以及制备外消旋氨基酸的方法，其包括使转化体或其处理物质的培养物作为酶源，并且氨基酸存在于水性介质中以使水性介质中的氨基酸外消旋化，并回收来自水介质的外消旋氨基酸。

6. 发明授权

US07553636B2 Surface expression vectors having pgsBCA the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector 有权
标题翻译：具有编码聚-γ-谷氨酸合成酶的基因的pgsBCA的表达表达载体，以及使用载体在微生物表面表达靶蛋白的方法
公开(公告)号：US07553636B2
公开(公告)日：2009-06-30
申请号：US10484605
申请日：2002-08-09
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Chul-Joong Kim , Kenji Soda , Makoto Ashiuchi
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Chul-Joong Kim , Kenji Soda , Makoto Ashiuchi
IPC分类号： C12N15/09 , C12N1/21 , C12N15/63
CPC分类号： C12N9/1051 , C07K2319/02 , C12N9/1288 , C12Y204/01133 , C12Y207/08005
摘要： The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.
摘要翻译：本发明涉及具有pgsBCA的表达表达载体，编码聚-γ-谷氨酸合成酶的基因和使用该载体在微生物表面表达靶蛋白的方法。插入外来基因的载体转化微生物，使外源蛋白稳定表达在微生物表面。

7. 发明申请

US20050095670A1 Amino acid racemase having low substrate specificity and process for producing racemic amino acid 有权
标题翻译：具有低底物特异性的氨基酸消旋酶和用于生产外消旋氨基酸的方法
公开(公告)号：US20050095670A1
公开(公告)日：2005-05-05
申请号：US10506152
申请日：2003-02-28
申请人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto , Makoto Yagasaki , Kenji Soda
发明人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto , Makoto Yagasaki , Kenji Soda
IPC分类号： C12N1/15 , C12N1/19 , C12N1/21 , C12N9/90 , C12N15/61 , C12P13/04 , C07H21/04 , C12N15/74 , C12P21/06
CPC分类号： C12P13/04 , C12N9/90
摘要： The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.
摘要翻译：本发明提供了具有低底物特异性氨基酸消旋酶活性的蛋白质; 编码蛋白质的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 通过使用转化体生产蛋白质的方法; 以及制备外消旋氨基酸的方法，其包括使转化体或其处理物质的培养物作为酶源，并且氨基酸存在于水性介质中以使水性介质中的氨基酸外消旋化，并回收来自水介质的外消旋氨基酸。

8. 发明授权

US07666653B2 Low-substrate-specific amino acid racemase and process for producing racemic amino acid 失效
标题翻译：低底物特异性氨基酸消旋酶及其制备外消旋氨基酸的方法
公开(公告)号：US07666653B2
公开(公告)日：2010-02-23
申请号：US11808186
申请日：2007-06-07
申请人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto , Makoto Yagasaki , Kenji Soda
发明人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto , Makoto Yagasaki , Kenji Soda
IPC分类号： C07H21/04 , C12N9/90 , C12N15/74 , C12N1/21 , C12P21/06
CPC分类号： C12P13/04 , C12N9/90
摘要： The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.
摘要翻译：本发明提供了具有低底物特异性氨基酸消旋酶活性的蛋白质; 编码蛋白质的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 通过使用转化体生产蛋白质的方法; 以及制备外消旋氨基酸的方法，其包括使转化体或其处理物质的培养物作为酶源，并且氨基酸存在于水性介质中以使水性介质中的氨基酸外消旋化，并回收来自水介质的外消旋氨基酸。

9. 发明申请

US20060127447A1 Poly-gamma-glutamate having ultra high molecular weight and method for using the same 审中-公开
标题翻译：具有超高分子量的聚-γ-谷氨酸盐及其使用方法
公开(公告)号：US20060127447A1
公开(公告)日：2006-06-15
申请号：US10520557
申请日：2003-07-10
申请人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Kwang Seok Kim , Jae Jun Song , Eu Gene Rha , Kwang Kim , Kenji Soda , Makoto Ashiuchi , Ha Ryoung Poo , Seung Lee
发明人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Kwang Seok Kim , Jae Jun Song , Eu Gene Rha , Kwang Kim , Kenji Soda , Makoto Ashiuchi , Ha Ryoung Poo , Seung Lee
IPC分类号： A61K38/16 , A61K47/00 , C07K14/32 , C12N1/21 , C12P21/06
CPC分类号： A61K9/0014 , A61K8/88 , A61K9/0095 , A61K47/42 , A61Q19/00 , C08H1/00 , C12P21/02
摘要： The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95% of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.
摘要翻译：本发明涉及超高分子量大于5,000kDa的聚-γ-谷氨酸（PGA）。根据本发明的超高分子量PGA的平均分子量大于13000kDa，其分子的95％以上具有3,000至15,000kDa的分子量。此外，它可以通过培养枯草芽孢杆菌变种产生。 ung ko ang。根据本发明的超高分子量PGA显示出非常优异的吸湿性，保湿性，缓释性，矿物溶解性和吸水性，因此可用作新的高附加值材料在各种应用中。

10. 发明授权

US4335209A Process for preparation of L-tryptophan by enzyme 失效
标题翻译：通过酶制备L-色氨酸的方法
公开(公告)号：US4335209A
公开(公告)日：1982-06-15
申请号：US145267
申请日：1980-04-25
申请人： Yoshiyuki Asai , Masao Shimada , Kenji Soda
发明人： Yoshiyuki Asai , Masao Shimada , Kenji Soda
IPC分类号： C07D209/20 , C12N9/88 , C12N9/90 , C12P13/06 , C12P13/22
CPC分类号： C12P13/06 , C07D209/20 , C12N9/88 , C12N9/90 , C12P13/227
摘要： A process for the preparation of L-tryptophan comprises reacting indole with serine in the presence of tryptophan synthetase or tryptophanase, wherein DL-serine or D-serine is used and a serine racemizing enzyme is included in the reaction system and reacted with the serine.
摘要翻译：制备L-色氨酸的方法包括在色氨酸合成酶或色氨酸酶存在下使吲哚与丝氨酸反应，其中使用DL-丝氨酸或D-丝氨酸，并且丝氨酸外消旋酶包含在反应体系中并与丝氨酸反应。

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