专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20050239100A1 Method for amplifying nucleic acid sequence 审中-公开
标题翻译：扩增核酸序列的方法
公开(公告)号：US20050239100A1
公开(公告)日：2005-10-27
申请号：US10973919
申请日：2004-10-27
申请人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C07H21/04 , C12N9/22 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2521/301
摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
摘要翻译：一种用于扩增核酸序列的方法和方法，其特征在于在嵌合寡核苷酸引物存在下进行DNA合成反应; 提供大量DNA扩增片段的方法; 通过将上述方法与另一种核酸序列扩增方法结合来扩增核酸序列的有效方法; 用于检测用于检测或定量微生物如病毒，细菌，真菌或酵母的核酸序列的方法; 以及通过上述方法原位检测DNA扩增片段的方法。

2. 发明申请

US20050123950A1 Method for amplifying nucleic acid sequence 审中-公开
公开(公告)号：US20050123950A1
公开(公告)日：2005-06-09
申请号：US10929759
申请日：2004-08-31
申请人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C07H21/04 , C12N9/22 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2521/301
摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

3. 发明授权

US06951722B2 Method for amplifying nucleic acid sequence 失效
公开(公告)号：US06951722B2
公开(公告)日：2005-10-04
申请号：US09935338
申请日：2001-08-23
申请人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroyuki Mukai , Hiroaki Sagawa , Takashi Uemori , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Osamu Takeda , Kazue Miyake , Yoshimi Sato , Mariko Moriyama , Haruhisa Sawaragi , Michio Hagiya , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C07H21/04 , C12N9/22 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6853 , C12Q2531/119 , C12Q2525/121 , C12Q2521/301
摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

4. 发明授权

US06673578B1 Method for synthesizing DNA 失效
标题翻译： DNA合成方法
公开(公告)号：US06673578B1
公开(公告)日：2004-01-06
申请号：US09786684
申请日：2001-05-22
申请人： Takashi Uemori , Yoshimi Sato , Mariko Okawa , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroaki Sagawa , Michio Hagiya , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Mariko Okawa , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroaki Sagawa , Michio Hagiya , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12P1934
CPC分类号： C12N15/1003 , C12Q1/686 , C12Q2527/125
摘要： A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 &mgr;l of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 &mgr;l volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99° C., 1 second-66° C., 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent. According to the present invention, the procedures in the genetic engineering studies and industries involved with PCR can be speeded up.
摘要翻译：一种DNA合成方法，其通过聚合酶链式反应（PCR）进行DNA合成所需的时间缩短，其特征在于使用DNA聚合酶，其量有效提供超过10ng每50ul约2kb的扩增DNA片段的反应混合物，当在以下条件（A）和（B）下进行PCR时：（A）反应混合物：将50体积的包含DNA聚合酶的反应混合物，1ng来自大肠杆菌的基因组DNA和10ng 各引物Eco-1和Eco-2（引物Eco-1和Eco-2的核苷酸序列分别示于序列表的SEQ ID NO：10和11中）并具有适合于DNA聚合酶的组合物; 和（B）反应条件：35个循环的PCR，其中一个循环由99℃，1秒-66℃，7秒; 用于DNA合成方法的DNA合成试剂盒; 以及PCR试剂的制造。根据本发明，可以加快基因工程研究和涉及PCR的行业的程序。

5. 发明申请

US20050059000A1 Method of stabilizing reagent for amplifying or detecting nucleic acid and storage method 审中-公开
标题翻译：稳定核酸扩增或检测试剂的方法及储存方法
公开(公告)号：US20050059000A1
公开(公告)日：2005-03-17
申请号：US10478633
申请日：2002-06-12
申请人： Hiroaki Sagawa , Takashi Uemori , Hiroyuki Mukai , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroaki Sagawa , Takashi Uemori , Hiroyuki Mukai , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2521/327 , C12Q2525/121 , C12Q2527/137
摘要： A method of stabilizing a reaction reagent for highly sensitively and specifically amplifying a target nucleic acid in a sample with the use of a chimeric oligonucleotide primer and a method of storing the same over a long time; and a method of highly sensitively detecting a pathogenic microorganism and a virus.
摘要翻译：使用嵌合寡核苷酸引物对样品中的靶核酸进行高灵敏度和特异性扩增的反应试剂的稳定化方法及其长时间存储的方法; 以及高敏感性检测病原微生物和病毒的方法。

6. 发明申请

US20050118578A1 Amplified nucleic acids and immobilized products thereof 审中-公开
标题翻译：扩增核酸及其固定化产物
公开(公告)号：US20050118578A1
公开(公告)日：2005-06-02
申请号：US10467325
申请日：2002-02-06
申请人： Junichi Mineno , Osamu Takeda , Masatomo Rokushima , Takashi Uemori , Shigekazu Hokazono , Hiroyuki Mukai , Shusaku Yamashita , Hiroaki Sagawa , Kiyozo Asada , Ikunoshin Kato
发明人： Junichi Mineno , Osamu Takeda , Masatomo Rokushima , Takashi Uemori , Shigekazu Hokazono , Hiroyuki Mukai , Shusaku Yamashita , Hiroaki Sagawa , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q2565/518 , C12Q2531/119
摘要： A nucleic acid, which is provided in a large amount through a nucleic acid amplification reaction with the use of chimeric oligonucleotide primers, is constructed in a state of containing a modified deoxyribonucleotide for immobilizing the nucleic acid to a solid phase and then immobilized to a solid phase at a high efficiency, thereby giving an immobilized nucleic acid product with excellent qualities.
摘要翻译：通过使用嵌合寡核苷酸引物通过核酸扩增反应大量提供的核酸以含有修饰的脱氧核糖核苷酸的状态构建，用于将核酸固定至固相，然后固定于固体相，从而得到具有优异品质的固定化核酸产物。

7. 发明申请

US20070037139A1 Method of analyzing gene introduction site 审中-公开
标题翻译：分析基因引入位点的方法
公开(公告)号：US20070037139A1
公开(公告)日：2007-02-15
申请号：US10555876
申请日：2004-05-07
申请人： Jun Tomono , Harumi Ueno , Osamu Takeda , Hiroyuki Mukai , Hiroaki Sagawa , Ikunoshin Kato
发明人： Jun Tomono , Harumi Ueno , Osamu Takeda , Hiroyuki Mukai , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12Q1/70 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q2527/107
摘要： A method of analyzing, in chromosomal DNA having a retroviral vector incorporated therein, DNA region derived from chromosome which is adjacent to the vector, characterized in that a primer extension reaction and a nucleic acid amplification reaction are carried out in the presence of a compound capable of lowering the Tm value of double stranded nucleic acid. There are further provided a kit and buffer for use in this method.
摘要翻译：一种在其中并入有逆转录病毒载体的染色体DNA中分析衍生自与载体相邻的染色体的DNA区域的方法，其特征在于引物延伸反应和核酸扩增反应在具有能力的化合物存在下进行降低双链核酸的Tm值。此外还提供了用于该方法的试剂盒和缓冲液。

8. 发明授权

US06177592B1 Compounds 失效
标题翻译：化合物
公开(公告)号：US06177592B1
公开(公告)日：2001-01-23
申请号：US09341768
申请日：1999-07-16
申请人： Nobuto Koyama , Tatsuji Enoki , Katsushige Ikai , Hua-Kang Wu , Hiromu Ohnogi , Takanari Tominaga , Eiji Nishiyama , Michio Hagiya , Hiroaki Sagawa , Hideto Chono , Ikunoshin Kato
发明人： Nobuto Koyama , Tatsuji Enoki , Katsushige Ikai , Hua-Kang Wu , Hiromu Ohnogi , Takanari Tominaga , Eiji Nishiyama , Michio Hagiya , Hiroaki Sagawa , Hideto Chono , Ikunoshin Kato
IPC分类号： C07C6106
CPC分类号： C07C323/59 , A23K20/105 , A23L2/52 , A23L33/10 , A23V2002/00 , A61K31/122 , C07C45/60 , C07C45/78 , C07C49/707 , C07C323/58 , C07C2601/08 , Y02A50/478 , A23V2200/224 , A23V2250/5026 , A23V2250/5024 , A23V2250/70 , A23V2250/204 , A23V2250/06 , A23V2250/21
摘要： A compound represented by the following formula [I] or an optically active substance or a salt thereof. (In the formula, a bond shown by a dotted line in the five-membered ring means that said five-membered ring may be any of a cyclopentene ring having a double bond and a cyclopentane ring where said bond is saturated and, in the case of a cyclopentene ring, X is OH, Y is ═O and Z is H while, in the case of a cyclopentane ring, X is ═O, Y is OH and Z is OH. R is a residue after removal of an SH group from the SH-containing compound.)
摘要翻译：由下式[I]表示的化合物或光学活性物质或其盐（在该式中，五元环中虚线所示的键表示所述五元环可以是具有双键的环戊烯环和所述键饱和的环戊烷环，在环戊烯环的情况下，X为OH，Y为= O且Z为H，而在环戊烷环的情况下，X为= O，Y是OH，Z是OH，R是从含SH的化合物除去SH基团后的残基。

9. 发明授权

US06608032B1 Medicinal compositions 失效
标题翻译：药用成分
公开(公告)号：US06608032B1
公开(公告)日：2003-08-19
申请号：US09889732
申请日：2001-07-20
申请人： Tatsuji Enoki , Hiroaki Sagawa , Jun Tomono , Takanari Tominaga , Eiji Nishiyama , Hua-Kang Wu , Ikunoshin Kato
发明人： Tatsuji Enoki , Hiroaki Sagawa , Jun Tomono , Takanari Tominaga , Eiji Nishiyama , Hua-Kang Wu , Ikunoshin Kato
IPC分类号： A01N4304
CPC分类号： A61K31/7048 , A61K31/7004 , A61K31/7016 , A61K31/702 , A61K31/7024 , A61K31/7028 , C07H3/10 , C07H11/00 , C07H15/04 , Y02A50/411 , Y10S977/915
摘要： Medicinal compositions for treating or preventing diabetes, rheumatoid, diseases wherein inflammation should be inhibited, diseases wherein &agr;-glycosidase should be inhibited, diseases wherein the synthesis of prostaglandin should be inhibited, diseases wherein endotoxin shock should be inhibited, diseases wherein the production of interleukin should be inhibited, diseases wherein the production of heme oxygenase should be induced, and diseases wherein the production of tumor necrosis factor or carcinogenesis should be inhibited, which contain as the active ingredient at least one compound selected from the group consisting of 3,6-anhydrogalactopyranose represented by formula (I), its aldehyde, its hydrate and 2-O-methylated derivatives and 2-O-sulfated derivatives thereof.
摘要翻译：用于治疗或预防糖尿病，类风湿病，其中应该抑制炎症的疾病的药物组合物，应该抑制α-糖苷酶的疾病，应该抑制前列腺素合成的疾病，应该抑制内毒素休克的疾病，其中产生白细胞介素应该被抑制，其中应该诱导血红素加氧酶的产生的疾病，以及应该抑制肿瘤坏死因子或致癌作用的产生的疾病，其中含有选自下组的至少一种化合物作为活性成分：由式（I）表示的脱水吡喃半乳糖，其醛，其水合物和2-O-甲基化衍生物及其2-O-硫酸化衍生物。

10. 发明授权

US06531148B1 Therapeutic agents 失效
标题翻译：治疗剂
公开(公告)号：US06531148B1
公开(公告)日：2003-03-11
申请号：US09719314
申请日：2001-02-01
申请人： Tatsuji Enoki , Jun Tomono , Nobuto Koyama , Katsushige Ikai , Hiroaki Sagawa , Ikunoshin Kato
发明人： Tatsuji Enoki , Jun Tomono , Nobuto Koyama , Katsushige Ikai , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： A61K4700
CPC分类号： A61K31/7004 , A23L3/3535 , A23L3/3562 , A23L33/10 , A23V2002/00 , A61K31/35 , A61K31/351 , A61K31/357 , A23V2250/60 , A23V2250/608 , A23V2250/5024 , A23V2250/5036
摘要： Therapeutic or preventive agents for diseases requiring apoptosis induction, cancerous diseases, diseases requiring the inhibition of active oxygen production, those requiring the inhibition of nitrogen monoxide production, those requiring the inhibition of prostaglandin synthesis, those requiring the inhibition of synovial cell proliferation, those requiring the induction of heat shock protein production or those requiring the inhibition of &agr;-glycosidase, which contain as the active ingredient compounds selected from among compounds represented by general formula (I), (wherein X and Y are each H or CH2OH, provided that when X is CH2OH, Y is H, while when X is H, Y is CH2OH), those represented by general formula (II), (wherein R is a residue obtained by freeing a compound having an SH group from the SH group) and salts of both; and foods, drinks, cosmetics and so on, containing compounds selected from among compounds of general formula (I), those of general formula (II) and salts of both.
摘要翻译：需要细胞凋亡诱导的疾病，癌性疾病，需要抑制活性氧产生的疾病，需要抑制一氧化氮生成的那些的治疗或预防剂，需要抑制前列腺素合成的那些，需要抑制滑膜细胞增殖的那些，需要诱导热休克蛋白产生或需要抑制α-糖苷酶的那些，其含有选自通式（I）表示的化合物中的活性成分，其中X和Y各自为H或CH 2 OH，条件是当 X是CH 2 OH，Y是H，而X是H，Y是CH 2 OH），由通式（II）表示的那些（其中R是从SH基团中释放具有SH基团的化合物获得的残基）的两个和食品，饮料，化妆品等，其含有选自通式（I）的化合物，通式（II）的化合物和二者的盐的化合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式