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1. 发明授权

US07198890B2 Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine 有权
标题翻译：用于定量测定同型半胱氨酸的方法和用于定量测定同型半胱氨酸的试剂
公开(公告)号：US07198890B2
公开(公告)日：2007-04-03
申请号：US10755236
申请日：2004-01-13
申请人： Hiroyuki Ebinuma , Takami Sarashina , Takuji Matsumoto , Masahiro Sekiguchi , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Takami Sarashina , Takuji Matsumoto , Masahiro Sekiguchi , Koji Ushizawa
IPC分类号： C12Q1/00 , C12N9/00 , C07K1/00
CPC分类号： G01N33/6812 , G01N2333/90241
摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.
摘要翻译：本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。

2. 发明申请

US20050153385A1 Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine 有权
标题翻译：用于定量测定同型半胱氨酸的方法和用于定量测定同型半胱氨酸的试剂
公开(公告)号：US20050153385A1
公开(公告)日：2005-07-14
申请号：US10755236
申请日：2004-01-13
申请人： Hiroyuki Ebinuma , Takami Sarashina , Takuji Matsumoto , Masahiro Sekiguchi , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Takami Sarashina , Takuji Matsumoto , Masahiro Sekiguchi , Koji Ushizawa
IPC分类号： C12Q1/26 , C12Q1/527 , G01N33/53 , G01N33/68
CPC分类号： G01N33/6812 , G01N2333/90241
摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.
摘要翻译：本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。

3. 发明授权

US5871949A Method of quantitative assay for 1,5-anhydroglucitol and reagent for quantitative assay 失效
标题翻译：定量测定1,5-脱水葡萄糖醇和试剂的方法
公开(公告)号：US5871949A
公开(公告)日：1999-02-16
申请号：US980792
申请日：1997-12-01
申请人： Hiroyuki Ebinuma , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Koji Ushizawa
IPC分类号： C12Q1/32 , C12Q1/00 , C12Q1/54
CPC分类号： C12Q1/32
摘要： Using a 1,5-anhydroglucitol dehydrogenase capable of acting on 1,5-anhydroglucitol and directly catalyzing a reducing chromophoric agent in the absence of an electron carrier, the 1,5-anhydroglucitol dehydrogenase is allowed to act on 1,5-anhydroglucitol in the presence of the reducing chromophoric agent preferably after the glucose in the specimen has been changed, or while being changed, into such a structure that it does not react with the 1,5-anhydroglucitol dehydrogenase in the specimen, by the aid of a glucose eliminator. Then, the amount of the resultant reduced colored substance is measured. As the 1,5-anhydroglucitol dehydrogenase, an enzyme produced by a microorganism having the ability to produce the 1,5-anhydroglucitol dehydrogenase is preferably used.
摘要翻译：使用能够作用于1,5-脱水葡萄糖醇并在不存在电子载体的情况下直接催化还原性发色剂的1,5-脱水葡萄糖醇脱氢酶，使1,5-脱水葡萄糖醇脱氢酶作用于1,5-脱水葡萄糖醇还原性发色剂的存在优选在样品中的葡萄糖已经被改变或改变之后，使其不与样品中的1,5-脱水葡萄糖醇脱氢酶反应，借助于葡萄糖消除者然后，测量所得的还原着色物质的量。作为1,5-脱水葡萄糖醇脱氢酶，优选使用具有生产1,5-脱水葡萄糖醇脱氢酶能力的微生物产生的酶。

4. 发明授权

US06541215B1 Quantitative determination method of mannose and reagent therefor 有权
标题翻译：甘露糖及其试剂的定量测定方法
公开(公告)号：US06541215B1
公开(公告)日：2003-04-01
申请号：US09708454
申请日：2000-11-09
申请人： Hiroyuki Ebinuma , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Koji Ushizawa
IPC分类号： C12Q126
CPC分类号： C12Q1/32 , Y10S435/823 , Y10S435/962
摘要： Disclosed are a method for quantitatively determining mannose, which comprises reacting mannose in a specimen with an enzyme which is capable of oxidizing the mannose by dehydrogenation, in the presence of an electron acceptor, and quantitatively determining a formed reductant of the electron acceptor; and a reagent for the quantitative determination of mannose.
摘要翻译：本发明公开了一种定量测定甘露糖的方法，其包括使试样中的甘露糖与能够通过脱氢氧化甘露糖的酶在电子受体存在下反应并定量测定形成的电子受体的还原剂; 和用于定量测定甘露糖的试剂。

5. 发明授权

US06969613B1 Method for determination of hydrogen sulfide or sulfide ion and method for determination of specific substance utilizing said method 有权
标题翻译：用于测定硫化氢或硫化物离子的方法和利用所述方法测定特定物质的方法
公开(公告)号：US06969613B1
公开(公告)日：2005-11-29
申请号：US09856790
申请日：1999-12-07
申请人： Hiroyuki Ebinuma , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Koji Ushizawa
IPC分类号： C12Q1/527 , G01N31/22 , G01N33/00
CPC分类号： C12Q1/527 , G01N31/22 , G01N31/224 , G01N33/0044 , Y10T436/18 , Y10T436/182
摘要： The present invention provides a method for quantitatively determining hydrogen sulfide or sulfide ions conveniently with high sensitivity, which comprises adding to a sample containing hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator. The present invention further provides a method for quantitatively determining a specific substance, which comprises adding to a sample containing a specific substance, a component which acts on the specific substance so that the specific substance forms hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions, and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator.
摘要翻译：本发明提供了一种以高灵敏度方便地定量测定硫化氢或硫化物离子的方法，其包括向含有硫化氢或硫化物离子的金属离子或释放所述金属离子的化合物和与金属离子发生显色，其中显色被硫化氢或硫化物离子加速或抑制; 并测量金属指示器的显色程度。本发明还提供了一种定量测定特定物质的方法，该方法包括向含有特定物质的样品中加入作用于特定物质的成分，使特定物质形成硫化氢或硫化物离子，金属离子或化合物释放出所述金属离子的金属指示剂和与金属离子反应并导致发色的金属指示剂，其中显色被硫化氢或硫化物离子加速或抑制; 并测量金属指示器的显色程度。

6. 发明授权

US06632677B1 Air shutoff agent for aqueous reagent and aqueous specimen, and method for storing aqueous reagent and aqueous specimen with stability 有权
标题翻译：水性试剂和水性试样的空气截止剂，以及稳定地储存水性试剂和含水试样的方法
公开(公告)号：US06632677B1
公开(公告)日：2003-10-14
申请号：US09786749
申请日：2001-03-09
申请人： Masahiro Sekiguchi , Toshikatsu Abe , Koji Ushizawa
发明人： Masahiro Sekiguchi , Toshikatsu Abe , Koji Ushizawa
IPC分类号： G01N3100
CPC分类号： B01J19/16 , C08K5/01 , G01N2035/00277 , G01N2035/00287 , Y02P20/149 , Y10T436/10 , Y10T436/108331 , Y10T436/21 , Y10T436/214 , Y10T436/25 , Y10T436/2525 , C08L83/04
摘要： An air-barrier agent for an aqueous reagent or an aqueous specimen having as an effective component a mixture of a chain hydrocarbon and a silicone oil immiscible with the aqueous reagent as well methods of using and making the same.
摘要翻译：作为水性试剂的空气阻隔剂或具有与含水试剂不混溶的链烃和硅油的混合物作为有效成分的水性试剂，以及使用和制造其的方法。

7. 发明申请

US20070015141A1 Process for adjusting the diameter of gold particles and colloidal gold solution 审中-公开
标题翻译：调整金颗粒和胶体金溶液直径的方法
公开(公告)号：US20070015141A1
公开(公告)日：2007-01-18
申请号：US11483883
申请日：2006-07-10
申请人： Sachiko Ito , Michiko Kawamoto , Kiichiro Kobori , Koji Ushizawa
发明人： Sachiko Ito , Michiko Kawamoto , Kiichiro Kobori , Koji Ushizawa
IPC分类号： C12Q1/70 , G01N33/553
CPC分类号： G01N15/0205 , G01N33/585
摘要： A method for accurately determining the particle diameter of gold colloid in a simple manner, and a gold colloid solution with a particle diameter within a desired range are described. A method for estimating the particle diameter distribution width of gold colloid based on the measurement of, in addition to the maximum absorption wavelength, a ratio (AλX/Aλmax) of the absorbance (AλX) at a wavelength differing from the maximum absorption wavelength to the absorbance (Aλmax) at the maximum absorption wavelength of the gold colloid solution, and the gold colloid solution obtained by the method are provided.
摘要翻译：描述了以简单的方式精确测定金胶体的粒径的方法和粒径在期望范围内的金胶体溶液。基于除了最大吸收波长之外的测量，用于估计金胶体的粒径分布宽度的方法，其比例（λmax / 在金胶体溶液的最大吸收波长处，与最大吸收波长不同于吸光度（λmax max）的波长处的吸光度（Al x X S）和金提供了通过该方法获得的胶体溶液。

8. 发明申请

US20140349284A1 SAMPLE NUCLEIC ACID FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES, PCR PRIMER FOR PREPARING SAMPLE FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES, AND METHOD FOR PREPARING SAMPLE FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES WHICH CAN BE USED IN ION EXCHANGE CHROMATOGRAPHIC ANALYSIS 审中-公开
标题翻译：用于单核多核苷酸多态性检测目的的样品核酸，用于制备单核多核苷酸多态性检测目的的样品的PCR引物和用于在离子交换色谱分析中可以使用的单核多核苷酸多态性检测方法的样品的制备方法
公开(公告)号：US20140349284A1
公开(公告)日：2014-11-27
申请号：US14008770
申请日：2012-03-30
申请人： Takuya Yotani , Eiji Kiyotoh , Koji Ushizawa
发明人： Takuya Yotani , Eiji Kiyotoh , Koji Ushizawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6858 , C12Q2565/137
摘要： An object of the present invention is to provide a sample nucleic acid for single nucleotide polymorphism detection which is for use in a simple method for quickly detecting a single nucleotide polymorphism, PCR primers for preparation of a sample for single nucleotide polymorphism detection, and a sample for single nucleotide polymorphism detection which is for use in ion exchange chromatography analysis. The present invention provides a sample nucleic acid for single nucleotide polymorphism detection having the following features: (a) a full chain length of 200 bp or less; (b) a sequence (a tag sequence) incompletely complementary to a template DNA at the 5′ or 3′ end; and (c) a 10 bp or less difference in chain length from a sample nucleic acid to be compared with to determine the presence of a single nucleotide polymorphism.
摘要翻译：本发明的目的是提供用于单核苷酸多态性快速检测的简单方法的单核苷酸多态性检测用样品核酸，用于制备单核苷酸多态性检测用样品的PCR引物和样品用于单核苷酸多态性检测，用于离子交换色谱分析。本发明提供了用于单核苷酸多态性检测的样品核酸，其具有以下特征：（a）200bp或更短的完整链长度; （b）在5'或3'末端与模板DNA不完全互补的序列（标签序列）和（c）与要比较的样品核酸的链长的差异为10bp或更小，以确定单核苷酸多态性的存在。

9. 发明申请

US20070015140A1 Method of examining specimen and specimen container to be used in the examination method 有权
标题翻译：检查方法中使用的样本和样本容器的方法
公开(公告)号：US20070015140A1
公开(公告)日：2007-01-18
申请号：US10546691
申请日：2004-03-09
申请人： Kiichiro Kobori , Michiko Kawamoto , Koji Ushizawa
发明人： Kiichiro Kobori , Michiko Kawamoto , Koji Ushizawa
IPC分类号： C12Q1/70 , C07K16/10
CPC分类号： B01L3/50825 , B01L2300/046 , B01L2300/0681 , G01N33/5304 , G01N2333/11
摘要： A method of immunologically examining a specimen which comprises attaching a cap having a filter impregnated with a labeled antibody enclosed therein to a container body containing a diluted liquid specimen, pouring the diluted liquid specimen from the container into a test device, observing the reaction and thus examining the presence or absence of an analyte in the specimen. This examination method, whereby effects of differences among individual operators can be minimized and the occurrence of a nonspecific reaction can be prevented, is highly excellent in the reproducibility of the examination results and storage stability of a test reagent. A specimen container to be used in the above method to which a cap having a filter impregnated with a labeled antibody enclosed therein is attached. This container is appropriately usable as a member constituting a simplified diagnosis kit.
摘要翻译：一种免疫检查样品的方法，其包括将包含标记抗体的过滤器的盖子封装在包含稀释液体样品的容器体中，将稀释的液体样品从容器中倒入测试装置中，观察反应，从而检查样品中分析物的存在或不存在。这种检查方法能够将各个操作者之间的差异的效果最小化并且可以防止非特异性反应的发生，检查结果的再现性和试验试剂的储存稳定性都非常优异。在上述方法中使用的标本容器，其中附着有浸渍有标记抗体的过滤器的盖。该容器适合用作构成简化诊断试剂盒的部件。

10. 发明申请

US20140030713A1 FILLER FOR ION EXCHANGE CHROMATOGRAPHY AND METHOD FOR SEPARATING AND DETECTING NUCLEIC ACID STRAND 审中-公开
标题翻译：用于离子交换色谱的填料和分离和检测核酸条的方法
公开(公告)号：US20140030713A1
公开(公告)日：2014-01-30
申请号：US13984107
申请日：2012-02-09
申请人： Takuya Yotani , Koji Ushizawa
发明人： Takuya Yotani , Koji Ushizawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , B01D15/363 , B01J41/20 , C12N15/1006 , G01N30/96 , G01N2030/8827 , B01J41/05 , B01J41/07
摘要： The present invention aims to provide a filler for ion exchange chromatography which can sufficiently detect nucleic acid chains that differ in sequence of bases or nucleic acid chains that differ by a single base substitution. The present invention also aims to provide a method for separating and detecting a nucleic acid chain using the filler for ion exchange chromatography. The present invention relates to filler for ion exchange chromatography, comprising base fine particles, each particle having a strong cationic group and a weak cationic group on the surface thereof.
摘要翻译：本发明的目的是提供一种用于离子交换色谱的填充剂，其可以充分地检测由单碱基取代而不同的碱基或核酸链序列不同的核酸链。本发明还旨在提供一种使用离子交换层析用填料分离检测核酸链的方法。本发明涉及用于离子交换层析的填料，其包括基础细颗粒，其表面上具有强阳离子基团和弱阳离子基团的每个颗粒。

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