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1. 发明申请

US20140349284A1 SAMPLE NUCLEIC ACID FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES, PCR PRIMER FOR PREPARING SAMPLE FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES, AND METHOD FOR PREPARING SAMPLE FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION PURPOSES WHICH CAN BE USED IN ION EXCHANGE CHROMATOGRAPHIC ANALYSIS 审中-公开
标题翻译：用于单核多核苷酸多态性检测目的的样品核酸，用于制备单核多核苷酸多态性检测目的的样品的PCR引物和用于在离子交换色谱分析中可以使用的单核多核苷酸多态性检测方法的样品的制备方法
公开(公告)号：US20140349284A1
公开(公告)日：2014-11-27
申请号：US14008770
申请日：2012-03-30
申请人： Takuya Yotani , Eiji Kiyotoh , Koji Ushizawa
发明人： Takuya Yotani , Eiji Kiyotoh , Koji Ushizawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6858 , C12Q2565/137
摘要： An object of the present invention is to provide a sample nucleic acid for single nucleotide polymorphism detection which is for use in a simple method for quickly detecting a single nucleotide polymorphism, PCR primers for preparation of a sample for single nucleotide polymorphism detection, and a sample for single nucleotide polymorphism detection which is for use in ion exchange chromatography analysis. The present invention provides a sample nucleic acid for single nucleotide polymorphism detection having the following features: (a) a full chain length of 200 bp or less; (b) a sequence (a tag sequence) incompletely complementary to a template DNA at the 5′ or 3′ end; and (c) a 10 bp or less difference in chain length from a sample nucleic acid to be compared with to determine the presence of a single nucleotide polymorphism.
摘要翻译：本发明的目的是提供用于单核苷酸多态性快速检测的简单方法的单核苷酸多态性检测用样品核酸，用于制备单核苷酸多态性检测用样品的PCR引物和样品用于单核苷酸多态性检测，用于离子交换色谱分析。本发明提供了用于单核苷酸多态性检测的样品核酸，其具有以下特征：（a）200bp或更短的完整链长度; （b）在5'或3'末端与模板DNA不完全互补的序列（标签序列）和（c）与要比较的样品核酸的链长的差异为10bp或更小，以确定单核苷酸多态性的存在。

2. 发明申请

US20140147842A1 METHOD FOR DETECTING SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：检测单核苷酸多态性的方法
公开(公告)号：US20140147842A1
公开(公告)日：2014-05-29
申请号：US13979256
申请日：2012-01-12
申请人： Takuya Yotani , Eiji Kiyotoh , Koji Ushizawa
发明人： Takuya Yotani , Eiji Kiyotoh , Koji Ushizawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6858 , C12Q2565/137
摘要： An object of the present invention is to provide a method for rapidly and simply detecting single nucleotide polymorphisms. The present invention is a method for detecting single nucleotide polymorphisms, comprising analyzing wild-type and mutant-type products amplified by an AS-PCR method using ion-exchange chromatography.
摘要翻译：本发明的目的是提供一种快速简单地检测单核苷酸多态性的方法。本发明是用于检测单核苷酸多态性的方法，其特征在于，包括分析通过使用离子交换色谱法的AS-PCR方法扩增的野生型和突变型产物。

3. 发明申请

US20140030713A1 FILLER FOR ION EXCHANGE CHROMATOGRAPHY AND METHOD FOR SEPARATING AND DETECTING NUCLEIC ACID STRAND 审中-公开
标题翻译：用于离子交换色谱的填料和分离和检测核酸条的方法
公开(公告)号：US20140030713A1
公开(公告)日：2014-01-30
申请号：US13984107
申请日：2012-02-09
申请人： Takuya Yotani , Koji Ushizawa
发明人： Takuya Yotani , Koji Ushizawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , B01D15/363 , B01J41/20 , C12N15/1006 , G01N30/96 , G01N2030/8827 , B01J41/05 , B01J41/07
摘要： The present invention aims to provide a filler for ion exchange chromatography which can sufficiently detect nucleic acid chains that differ in sequence of bases or nucleic acid chains that differ by a single base substitution. The present invention also aims to provide a method for separating and detecting a nucleic acid chain using the filler for ion exchange chromatography. The present invention relates to filler for ion exchange chromatography, comprising base fine particles, each particle having a strong cationic group and a weak cationic group on the surface thereof.
摘要翻译：本发明的目的是提供一种用于离子交换色谱的填充剂，其可以充分地检测由单碱基取代而不同的碱基或核酸链序列不同的核酸链。本发明还旨在提供一种使用离子交换层析用填料分离检测核酸链的方法。本发明涉及用于离子交换层析的填料，其包括基础细颗粒，其表面上具有强阳离子基团和弱阳离子基团的每个颗粒。

4. 发明授权

US09481881B2 Eluent for ion-exchange chromatography, and method of analyzing nucleic acid chains 有权
标题翻译：用于离子交换层析的洗脱液和分析核酸链的方法
公开(公告)号：US09481881B2
公开(公告)日：2016-11-01
申请号：US13979241
申请日：2012-01-12
申请人： Takuya Yotani , Koji Ushizawa
发明人： Takuya Yotani , Koji Ushizawa
IPC分类号： C07H21/00 , C12Q1/68 , B01D15/00 , C12N15/10 , G01N30/96 , G01N30/02
CPC分类号： C12N15/101 , C12Q1/6806 , G01N30/96 , Y10T436/143333
摘要： The present invention provides eluent for ion-exchange chromatography, wherein the eluent allows separation and detection of a target nucleic acid such as a PCR-amplified product, a restriction enzyme fragment of the PCR-amplified product, or a restriction enzyme fragment of a nucleic acid in a short time with high separation performance. The present invention also provides a method of analyzing nucleic acid chains by ion-exchange chromatography using the eluent. The present invention provides an eluent for ion-exchange chromatography comprising a guanidine salt derived from guanidine represented by the following formula (1):

5. 发明申请

US20130330735A1 ELUENT FOR ION-EXCHANGE CHROMATOGRAPHY, AND METHOD OF ANALYZING NUCLEIC ACID CHAINS 有权
标题翻译：离子交换色谱法和分析核酸链的方法
公开(公告)号：US20130330735A1
公开(公告)日：2013-12-12
申请号：US13979241
申请日：2012-01-12
申请人： Takuya Yotani , Koji Ushizawa
发明人： Takuya Yotani , Koji Ushizawa
IPC分类号： G01N30/96
CPC分类号： C12N15/101 , C12Q1/6806 , G01N30/96 , Y10T436/143333
摘要： The present invention provides eluent for ion-exchange chromatography, wherein the eluent allows separation and detection of a target nucleic acid such as a PCR-amplified product, a restriction enzyme fragment of the PCR-amplified product, or a restriction enzyme fragment of a nucleic acid in a short time with high separation performance. The present invention also provides a method of analyzing nucleic acid chains by ion-exchange chromatography using the eluent. The present invention provides an eluent for ion-exchange chromatography comprising a guanidine salt derived from guanidine represented by the following formula (1):
摘要翻译：本发明提供了用于离子交换层析的洗脱液，其中洗脱液允许分离和检测目标核酸，例如PCR扩增产物，PCR扩增产物的限制酶片段或核酸的限制酶片段酸在短时间内具有高分离性能。本发明还提供了使用洗脱液通过离子交换层析分析核酸链的方法。本发明提供了一种用于离子交换层析的洗脱液，其包含由下式（1）表示的胍衍生的胍盐：

6. 发明申请

US20110073539A1 LIQUID CHROMATOGRAPHY COMPONENT 审中-公开
标题翻译：液相色谱组分
公开(公告)号：US20110073539A1
公开(公告)日：2011-03-31
申请号：US12921218
申请日：2009-03-31
申请人： Takuya Yotani
发明人： Takuya Yotani
IPC分类号： B01D15/10 , G01N30/14
CPC分类号： B01D15/22 , B01J20/28004 , B01J2220/54 , G01N1/40 , G01N30/14 , G01N30/465 , G01N30/603 , G01N30/6069 , G01N2030/085 , G01N2030/146 , G01N2030/524
摘要： The present invention aims to provide a liquid chromatography component including a column and a prefilter, which is hard to cause an increase of supplied liquid pressure even when the measurement of a sample is repeated. The present invention is a liquid chromatography component, which includes: a column with filler particles filled therein; and a prefilter, the filler particles having an average particle size in the range of 2 to 20 μm, the prefilter having a filtering particle size in the range of ⅙ to ⅓ of the average particle size of the filler particles.
摘要翻译：本发明的目的在于提供一种包括柱和预过滤器的液相色谱成分，即使重复测定样品，也难以引起供给液压的增加。本发明是一种液相色谱组分，其包括：填充有填料颗粒的柱; 和预滤器，所述填料颗粒的平均粒度在2至20μm的范围内，所述预过滤器的过滤粒度在填料颗粒的平均粒度的±1/3的范围内。

7. 发明申请

US20100330689A1 HYDROPHILIC POLYMER MICROPARTICLE, FILLER FOR ION EXCHANGE LIQUID CHROMATOGRAPHY, AND METHOD FOR PRODUCTION OF FILLER FOR ION EXCHANGE LIQUID CHROMATOGRAPHY 有权
标题翻译：亲水聚合物微球，离子交换液相色谱填充剂，以及用于离子交换液相色谱法生产填料的方法
公开(公告)号：US20100330689A1
公开(公告)日：2010-12-30
申请号：US12875283
申请日：2010-09-03
申请人： Takuya Yotani , Makoto Takahara
发明人： Takuya Yotani , Makoto Takahara
IPC分类号： G01N30/02 , B01J49/00
CPC分类号： B01D15/362 , B01J20/264 , B01J39/26 , B01J2220/54 , B01J2220/58 , C08F220/26 , C08F220/58 , C08F222/1006
摘要： An object of the present invention is to provide a hydrophilic polymer microparticle which shows reduced swelling in an aqueous medium and has an excellent dispersibility in an aqueous medium, a filler for ion-exchange liquid chromatography which can effectively suppress non-specific adsorption of protein and the like, a method for analyzing glycosylated hemoglobin using the filler for ion-exchange liquid chromatography, a method for production of a filler for ion-exchange liquid chromatography that can maintain suppressive effects on swelling, non-specific adsorption and the like for a long period of time, a filler for ion-exchange liquid chromatography produced by the method for production of a filler for ion-exchange liquid chromatography, and a filler for ion-exchange liquid chromatography for glycosylated hemoglobin analysis.The present is a hydrophilic polymer microparticle, which has a DW/DA ratio of 2.0 or less, the DW representing a particle diameter of the hydrophilic polymer microparticle dispersed in water, the DA representing a particle diameter of the hydrophilic polymer microparticle dispersed in acetone, each of the particle diameters measured by a particle size distribution analyzer after the hydrophilic polymer microparticle is dispersed in water or acetone, subjected to irradiation with ultrasonic waves for 15 minutes, and allowed to stand at a temperature of 25° C. for 240 hours for equilibration; and has a contact angle with water of 70° or less, the contact angle measured using a contact angle measurement apparatus at a temperature of 25° C. by forming a droplet of pure water on a single layer of the hydrophilic polymer microparticles arranged with no space therebetween.
摘要翻译：本发明的目的是提供一种亲水性聚合物微粒，其在水性介质中的溶胀度降低，并且在水性介质中具有优异的分散性，用于离子交换液相色谱的填料可有效抑制蛋白质的非特异性吸附，使用离子交换液相色谱用填充剂分析糖化血红蛋白的方法，用于离子交换液相色谱法的填充剂的制造方法，能够长时间保持对溶胀，非特异性吸附等的抑制作用时间段，用于离子交换液相色谱法的填充剂的制造方法制造的离子交换液相色谱用填充剂和糖基化血红蛋白分析用离子交换液相色谱用填充剂。本发明是DW / DA比为2.0以下的亲水性聚合物微粒，DW表示分散在水中的亲水性聚合物微粒的粒径，DA表示分散在丙酮中的亲水性聚合物微粒的粒径，将亲水性聚合物微粒分散在水或丙酮中后，用超声波照射15分钟后，通过粒度分布分析仪测定各粒径，在25℃的温度下放置240小时，平衡并且与水的接触角为70°以下，使用接触角测量装置在25℃的温度下测量的接触角，通过在不排列的亲水性聚合物微粒的单层上形成纯水滴之间的空间。

8. 发明申请

US20090042307A1 Hydrophilic Polymer Microparticle, Filler for Ion Exchange Liquid Chromatography, and Method for Production of Filler for Ion Exchange Liquid Chromatography 有权
标题翻译：亲水性聚合物微粒，离子交换液相色谱填充剂和离子交换液相色谱填料的生产方法
公开(公告)号：US20090042307A1
公开(公告)日：2009-02-12
申请号：US12085936
申请日：2006-11-14
申请人： Takuya Yotani , Makoto Takahara
发明人： Takuya Yotani , Makoto Takahara
IPC分类号： G01N33/72 , B01D15/08 , B29C71/00 , B29C71/04 , B06B1/02
CPC分类号： B01D15/362 , B01J20/264 , B01J39/26 , B01J2220/54 , B01J2220/58 , C08F220/26 , C08F220/58 , C08F222/1006
摘要： An object of the present invention is to provide a hydrophilic polymer microparticle which shows reduced swelling in an aqueous medium and has an excellent dispersibility in an aqueous medium, a filler for ion-exchange liquid chromatography which can effectively suppress non-specific adsorption of protein and the like, a method for analyzing glycosylated hemoglobin using the filler for ion-exchange liquid chromatography, a method for production of a filler for ion-exchange liquid chromatography that can maintain suppressive effects on swelling, non-specific adsorption and the like for a long period of time, a filler for ion-exchange liquid chromatography produced by the method for production of a filler for ion-exchange liquid chromatography, and a filler for ion-exchange liquid chromatography for glycosylated hemoglobin analysis.The present is a hydrophilic polymer microparticle, which has a DW/DA ratio of 2.0 or less, the DW representing a particle diameter of the hydrophilic polymer microparticle dispersed in water, the DA representing a particle diameter of the hydrophilic polymer microparticle dispersed in acetone, each of the particle diameters measured by a particle size distribution analyzer after the hydrophilic polymer microparticle is dispersed in water or acetone, subjected to irradiation with ultrasonic waves for 15 minutes, and allowed to stand at a temperature of 25° C. for 240 hours for equilibration; and has a contact angle with water of 70° or less, the contact angle measured using a contact angle measurement apparatus at a temperature of 25° C. by forming a droplet of pure water on a single layer of the hydrophilic polymer microparticles arranged with no space therebetween.
摘要翻译：本发明的目的是提供一种亲水性聚合物微粒，其在水性介质中的溶胀度降低，并且在水性介质中具有优异的分散性，用于离子交换液相色谱的填料可有效抑制蛋白质的非特异性吸附，使用离子交换液相色谱用填充剂分析糖化血红蛋白的方法，用于离子交换液相色谱法的填充剂的制造方法，能够长时间保持对溶胀，非特异性吸附等的抑制作用时间段，用于离子交换液相色谱法的填充剂的制造方法制造的离子交换液相色谱用填充剂和糖基化血红蛋白分析用离子交换液相色谱用填充剂。本发明是DW / DA比为2.0以下的亲水性聚合物微粒，DW表示分散在水中的亲水性聚合物微粒的粒径，DA表示分散在丙酮中的亲水性聚合物微粒的粒径，将亲水性聚合物微粒分散在水或丙酮中后，用超声波照射15分钟后，通过粒度分布分析仪测定各粒径，在25℃的温度下放置240小时，平衡并且与水的接触角为70°以下，使用接触角测量装置在25℃的温度下测量的接触角，通过在不排列的亲水性聚合物微粒的单层上形成纯水滴之间的空间。

9. 发明授权

US07811453B2 Hydrophilic polymer microparticle, filler for ion exchange liquid chromatography, and method for production of filler for ion exchange liquid chromatography 有权
标题翻译：亲水性聚合物微粒，离子交换液相色谱用填充剂和离子交换液相色谱用填充剂的制造方法
公开(公告)号：US07811453B2
公开(公告)日：2010-10-12
申请号：US12085936
申请日：2006-11-14
申请人： Takuya Yotani , Makoto Takahara
发明人： Takuya Yotani , Makoto Takahara
IPC分类号： B01D15/08
CPC分类号： B01D15/362 , B01J20/264 , B01J39/26 , B01J2220/54 , B01J2220/58 , C08F220/26 , C08F220/58 , C08F222/1006
摘要： An object of the present invention is to provide a hydrophilic polymer microparticle which shows reduced swelling in an aqueous medium and has an excellent dispersibility in an aqueous medium, a filler for ion-exchange liquid chromatography which can effectively suppress non-specific adsorption of protein and the like, a method for analyzing glycosylated hemoglobin using the filler for ion-exchange liquid chromatography, a method for production of a filler for ion-exchange liquid chromatography that can maintain suppressive effects on swelling, non-specific adsorption and the like for a long period of time, a filler for ion-exchange liquid chromatography produced by the method for production of a filler for ion-exchange liquid chromatography, and a filler for ion-exchange liquid chromatography for glycosylated hemoglobin analysis.
摘要翻译：本发明的目的是提供一种亲水性聚合物微粒，其在水性介质中的溶胀度降低，并且在水性介质中具有优异的分散性，用于离子交换液相色谱的填料可有效抑制蛋白质的非特异性吸附，使用离子交换液相色谱用填充剂分析糖化血红蛋白的方法，用于离子交换液相色谱法的填充剂的制造方法，能够长时间保持对溶胀，非特异性吸附等的抑制作用时间段，用于离子交换液相色谱法的填充剂的制造方法制造的离子交换液相色谱用填充剂和糖基化血红蛋白分析用离子交换液相色谱用填充剂。

10. 发明授权

US09310342B2 Liquid chromatography apparatus and liquid chromatography 有权
标题翻译：液相色谱仪和液相色谱仪
公开(公告)号：US09310342B2
公开(公告)日：2016-04-12
申请号：US13122602
申请日：2009-10-06
申请人： Toshikatsu Sakai , Akira Sezaki , Takeshi Takagi , Takuya Yotani , Makoto Takahara , Takayuki Oka
发明人： Toshikatsu Sakai , Akira Sezaki , Takeshi Takagi , Takuya Yotani , Makoto Takahara , Takayuki Oka
IPC分类号： G01N30/24 , G01N30/26 , G01N30/20 , G01N30/88 , B01D15/16 , G01N30/74
CPC分类号： G01N30/24 , B01D15/16 , G01N30/26 , G01N30/34 , G01N30/74 , G01N2030/201 , G01N2030/202 , G01N2030/207 , G01N2030/8822
摘要： A liquid chromatography apparatus is provided with a sample preparation unit, a column that separates components of a sample, an eluent supplier that includes a feeder for supplying eluents to the column, a flow path directional valve capable of introducing fixed amounts of the sample and the eluents to the column, an analyzer for analyzing a test solution composed of the sample components separated by the column and one of the eluents, and a controller, wherein the eluent supplier supplies the eluents to the flow path directional valve in an unmixed state. As a result of employing this configuration, analysis time is shortened and eluent consumption is reduced.
摘要翻译：液相色谱装置具有样品制备单元，分离样品组分的柱，包括用于向柱供给洗脱液的供给器的洗脱液供给器，能够引入固定量的样品的流路切换阀和用于分析由柱分离的样品组分和洗脱液之一组成的测试溶液的分析器和控制器，其中洗脱剂供体以未混合的状态将洗脱液供应到流路换向阀。由于采用这种结构，分析时间缩短，洗脱液消耗减少。

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