专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07198905B2 Method of screening methods prediabetic state and screening reagent 有权
标题翻译：筛选方法前驱糖尿病状态和筛选试剂方法
公开(公告)号：US07198905B2
公开(公告)日：2007-04-03
申请号：US10471823
申请日：2002-03-01
申请人： Hiroyuki Ebinuma , Kazunori Saito , Hirohito Sone , Nobuhiro Yamada
发明人： Hiroyuki Ebinuma , Kazunori Saito , Hirohito Sone , Nobuhiro Yamada
IPC分类号： C12Q1/32
CPC分类号： G01N33/66
摘要： The present invention provides a screening method by which a prediabetic state can be more accurately determined and many test samples can be treated without physical burden on patients, and a diagnostic reagent used for it. By measuring a D-mannose concentration, preferably a D-mannose concentration and a glucose concentration, in a body fluid collected from a subject, and comparing them with their respective standard values, a patient in a prediabetic state can be detected. Further, the measurement of the mannose concentration is conducted by allowing an enzyme having an oxidizing activity on mannose by dehydrogenation to react on the mannose in a sample in the presence of an electron acceptor, and quantitatively determining the formed reductant of the electron acceptor.
摘要翻译：本发明提供了一种筛选方法，通过该方法能够更准确地确定前列腺状态，并且可以对患者进行治疗而不会对身体造成负担的许多测试样品以及用于其的诊断试剂。通过在从受试者收集的体液中测量D-甘露糖浓度，优选D-甘露糖浓度和葡萄糖浓度，并将其与它们各自的标准值进行比较，可以检测患有糖尿病状态的患者。此外，甘露糖浓度的测定是通过在电子受体的存在下，使通过脱氢使对甘露糖具有氧化活性的酶在样品中与甘露糖反应，并定量测定形成的电子受体的还原剂来进行的。

2. 发明授权

US07951553B2 Method of assaying glycated protein 有权
标题翻译：测定糖化蛋白的方法
公开(公告)号：US07951553B2
公开(公告)日：2011-05-31
申请号：US10580000
申请日：2004-11-18
申请人： Yuriko Taniguchi , Hiroyuki Ebinuma , Kazunori Saito
发明人： Yuriko Taniguchi , Hiroyuki Ebinuma , Kazunori Saito
IPC分类号： C12Q1/37
CPC分类号： G01N33/726 , G01N33/6812 , G01N2333/95
摘要： The present invention provides a convenient, efficient method for assaying glycated protein, fructosyl peptide, or fructosyl amino acid which can be performed with reduced effect of fructosyl lysine compounds. The invention also provides a reagent for the assay.The invention is directed to a method for reducing the effect of a fructosyl lysine compound in an assay of fructosyl peptide or fructosyl amino acid contained in a sample, characterized by including causing an enzyme for assaying fructosyl peptide or fructosyl amino acid to act specifically on fructosyl peptide or fructosyl amino acid at a pH of 4.0 to 7.0 and measuring the product at a pH of 4.0 to 7.0; and a method for assaying glycated protein through the above method.
摘要翻译：本发明提供了一种用于测定糖基化蛋白质，果糖基肽或果糖基氨基酸的方便，有效的方法，其可以降低果糖基赖氨酸化合物的作用。本发明还提供了用于测定的试剂。本发明涉及一种降低果糖基赖氨酸化合物在样品中含有的果糖基肽或果糖基氨基酸的影响的方法，其特征在于包括使酶用于测定果糖基肽或果糖基氨基酸以特异性地作用于果糖基肽或果糖基氨基酸，pH为4.0〜7.0，在pH7.0〜7.0下测定产物; 以及通过上述方法测定糖化蛋白质的方法。

3. 发明申请

US20080233605A1 Method of Assaying Glycated Protein 有权
标题翻译：测定糖化蛋白的方法
公开(公告)号：US20080233605A1
公开(公告)日：2008-09-25
申请号：US10580000
申请日：2004-11-18
申请人： Yuriko Taniguchi , Hiroyuki Ebinuma , Kazunori Saito
发明人： Yuriko Taniguchi , Hiroyuki Ebinuma , Kazunori Saito
IPC分类号： C12Q1/37 , C12Q1/00
CPC分类号： G01N33/726 , G01N33/6812 , G01N2333/95
摘要： The present invention provides a convenient, efficient method for assaying glycated protein, fructosyl peptide, or fructosyl amino acid which can be performed with reduced effect of fructosyl lysine compounds. The invention also provides a reagent for the assay.The invention is directed to a method for reducing the effect of a fructosyl lysine compound in an assay of fructosyl peptide or fructosyl amino acid contained in a sample, characterized by including causing an enzyme for assaying fructosyl peptide or fructosyl amino acid to act specifically on fructosyl peptide or fructosyl amino acid at a pH of 4.0 to 7.0 and measuring the product at a pH of 4.0 to 7.0; and a method for assaying glycated protein through the above method.
摘要翻译：本发明提供了一种用于测定糖基化蛋白质，果糖基肽或果糖基氨基酸的方便，有效的方法，其可以降低果糖基赖氨酸化合物的作用。本发明还提供了用于测定的试剂。本发明涉及一种降低果糖基赖氨酸化合物在样品中含有的果糖基肽或果糖基氨基酸的影响的方法，其特征在于包括使酶用于测定果糖基肽或果糖基氨基酸以特异性地作用于果糖基肽或果糖基氨基酸，pH为4.0〜7.0，在pH7.0〜7.0下测定产物; 以及通过上述方法测定糖化蛋白质的方法。

4. 发明申请

US20140080158A1 METHOD FOR IMMUNOLOGICALLY MEASURING SOLUBLE LR11 有权
标题翻译：免疫测定方法LR11
公开(公告)号：US20140080158A1
公开(公告)日：2014-03-20
申请号：US14116683
申请日：2012-05-09
申请人： Kohei Takubo , Hiroyuki Ebinuma , Isamu Fukamachi , Hideaki Bujo , Yasushi Saito
发明人： Kohei Takubo , Hiroyuki Ebinuma , Isamu Fukamachi , Hideaki Bujo , Yasushi Saito
IPC分类号： G01N33/543
CPC分类号： G01N33/54393 , G01N33/92
摘要： To provide a method for assaying soluble LR11 in a biological sample, which method realizes a simple and accurate assay of soluble LR11 present in the sample by immunological means without requiring isolation of soluble LR11 from the biological sample (e.g., a serum sample).The method of the invention for immunologically assaying soluble LR11 present in a biological sample, characterized in that the method includes treating the sample with at least one surfactant selected from among one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants.
摘要翻译：为了提供用于测定生物样品中可溶性LR11的方法，该方法通过免疫学手段实现了样品中存在的可溶性LR11的简单且准确的测定，而不需要从生物样品（例如血清样品）分离可溶性LR11。用于免疫测定存在于生物样品中的可溶性LR11的本发明的方法，其特征在于所述方法包括用选自一种或多种磺基甜菜碱两性表面活性剂和一种或多种氨基磺基甜菜碱两性表面活性剂中的至少一种表面活性剂处理样品。

5. 发明授权

US07781171B2 Mehod of pretreating sample and immunological assay method using the same 有权
标题翻译：预处理样品的方法和使用其的免疫测定方法
公开(公告)号：US07781171B2
公开(公告)日：2010-08-24
申请号：US10575711
申请日：2004-10-15
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
IPC分类号： G01N33/53
CPC分类号： G01N33/74 , G01N2333/72 , Y10T436/25
摘要： The invention provides a method of pretreating a sample for conveniently, quickly and accurately measuring the total amount of adiponectin present in a biological sample contaminated with various adiponectin multimers.The method of measuring an sample for immunologically assaying the total amount of adiponectin present in the sample comprises reacting, with an adiponectin-containing sample, at least one of a reducing agent, an acid or a salt thereof, a surfactant, and a protease.
摘要翻译：本发明提供一种方法，用于方便，快速和准确地测量存在于受各种脂联素多聚体污染的生物样品中的脂连蛋白的总量的样品。用于免疫测定样品中存在的脂联素总量的样品的测定方法包括使含有脂联素的样品与还原剂，其酸或其盐，表面活性剂和蛋白酶中的至少一种反应。

6. 发明授权

US07244620B2 Method for quantitatively determining a reducing substance and a reagent for quantitative determination 有权
标题翻译：用于定量测定还原物质和定量测定试剂的方法
公开(公告)号：US07244620B2
公开(公告)日：2007-07-17
申请号：US10743741
申请日：2003-12-24
申请人： Masahiro Sekiguchi , Takuji Matsumoto , Hiroyuki Ebinuma
发明人： Masahiro Sekiguchi , Takuji Matsumoto , Hiroyuki Ebinuma
IPC分类号： G01N33/00 , G01N21/00 , G01N21/75 , C12Q1/00 , C12P11/00 , C12N9/88
CPC分类号： G01N31/22 , Y10T436/18 , Y10T436/182
摘要： The present invention provides a method for quantitatively determining a reducing substance, which comprises reacting a reducing substance in a test specimen with iron (III) ions, reacting iron (II) ions formed by reduction of the iron (III) ions or residual iron (III) ions with a metal indicator which is capable of reacting specifically with the iron (II) ions or the residual iron (III) ions to undergo color development, and carrying out quantitative determination by measuring the degree of color development, wherein a chelating agent which is specific to copper ions is added to the test specimen before the reaction of the reducing substance with the iron (III) ions; and a reagent used for it.
摘要翻译：本发明提供了一种定量测定还原物质的方法，该方法包括使试样中的还原物质与铁（III）离子反应，通过还原铁（III）离子或残余铁形成的铁（II） III）离子与能够与铁（II）离子或残留铁（III）离子特异性反应的金属指示剂进行显色，并通过测量显色程度进行定量测定，其中螯合剂在还原物质与铁（III）离子反应之前，将铜离子特异性添加到试样中; 和用于它的试剂。

7. 发明授权

US07198890B2 Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine 有权
标题翻译：用于定量测定同型半胱氨酸的方法和用于定量测定同型半胱氨酸的试剂
公开(公告)号：US07198890B2
公开(公告)日：2007-04-03
申请号：US10755236
申请日：2004-01-13
申请人： Hiroyuki Ebinuma , Takami Sarashina , Takuji Matsumoto , Masahiro Sekiguchi , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Takami Sarashina , Takuji Matsumoto , Masahiro Sekiguchi , Koji Ushizawa
IPC分类号： C12Q1/00 , C12N9/00 , C07K1/00
CPC分类号： G01N33/6812 , G01N2333/90241
摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.
摘要翻译：本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。

8. 发明申请

US20070054344A1 Novel fructosyl peptide oxidase and utilization thereof 有权
标题翻译：新型果糖基肽氧化酶及其利用
公开(公告)号：US20070054344A1
公开(公告)日：2007-03-08
申请号：US10531305
申请日：2003-10-23
申请人： Hiroyuki Ebinuma
发明人： Hiroyuki Ebinuma
IPC分类号： C12Q1/37 , C12P21/06 , C12N9/24
CPC分类号： C07K14/805 , C12N9/0022 , C12N9/2402 , C12P21/00 , C12P21/005 , C12Q1/26 , C12Q1/34 , G01N33/68 , G01N33/725 , G01N2400/00
摘要： The present invention is directed to a defructosylation enzyme originating from a plant, a method of defructosylating a fructosylated peptide or protein through use of the enzyme, and a method of measuring a fructosylated peptide or protein.
摘要翻译：本发明涉及来自植物的脱甲硅烷基化酶，通过使用酶使果糖基化肽或蛋白质去结构化的方法，以及测量果糖基化肽或蛋白质的方法。

9. 发明申请

US20050153385A1 Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine 有权
标题翻译：用于定量测定同型半胱氨酸的方法和用于定量测定同型半胱氨酸的试剂
公开(公告)号：US20050153385A1
公开(公告)日：2005-07-14
申请号：US10755236
申请日：2004-01-13
申请人： Hiroyuki Ebinuma , Takami Sarashina , Takuji Matsumoto , Masahiro Sekiguchi , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Takami Sarashina , Takuji Matsumoto , Masahiro Sekiguchi , Koji Ushizawa
IPC分类号： C12Q1/26 , C12Q1/527 , G01N33/53 , G01N33/68
CPC分类号： G01N33/6812 , G01N2333/90241
摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.
摘要翻译：本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。

10. 发明授权

US09541550B2 Method for immunologically measuring soluble LR11 有权
标题翻译：免疫测定可溶性LR11的方法
公开(公告)号：US09541550B2
公开(公告)日：2017-01-10
申请号：US14116683
申请日：2012-05-09
申请人： Kohei Takubo , Hiroyuki Ebinuma , Isamu Fukamachi , Hideaki Bujo , Yasushi Saito
发明人： Kohei Takubo , Hiroyuki Ebinuma , Isamu Fukamachi , Hideaki Bujo , Yasushi Saito
IPC分类号： G01N33/543 , G01N33/92
CPC分类号： G01N33/54393 , G01N33/92
摘要： To provide a method for assaying soluble LR11 in a biological sample, which method realizes a simple and accurate assay of soluble LR11 present in the sample by immunological means without requiring isolation of soluble LR11 from the biological sample (e.g., a serum sample).The method of the invention for immunologically assaying soluble LR11 present in a biological sample, characterized in that the method includes treating the sample with at least one surfactant selected from among one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants.
摘要翻译：用于免疫测定存在于生物样品中的可溶性LR11的本发明的方法，其特征在于所述方法包括用选自一种或多种磺基甜菜碱两性表面活性剂和一种或多种氨基磺基甜菜碱两性表面活性剂中的至少一种表面活性剂处理样品。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式