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    • 5. 发明授权
    • Vacuum booster apparatus and a brake apparatus
    • 真空增压装置和制动装置
    • US06412883B1
    • 2002-07-02
    • US10046144
    • 2002-01-16
    • Yasuji MizutaniMotoshi SuzukiHirofumi Nitta
    • Yasuji MizutaniMotoshi SuzukiHirofumi Nitta
    • B60T844
    • F02D9/02B60T8/4809B60T13/683B60T13/686B60T13/72
    • A vacuum booster apparatus used for a brake apparatus positively decreases a degree of opening of a throttle valve when it is required while an unnecessary decrease in the degree of opening of the throttle valve is positively prevented. A first pressure chamber of a vacuum booster is connected to an intake pipe of the engine on a downstream side of a throttle valve. A second pressure chamber of the vacuum booster is selectively connectable to one of the first pressure chamber and atmosphere. The vacuum booster boosts an operating force applied to a brake operating member based on a pressure difference between the first pressure chamber and the second pressure chamber. A throttle-opening-degree control device controls a negative pressure in the first pressure chamber by controlling the degree of opening of the throttle valve. The throttle-opening-degree control device decreases the degree of opening of the throttle valve only for a limited time when the negative pressure in the first pressure chamber is smaller than a predetermined necessary negative pressure determined by an operating-force relating amount related to the operating force applied to the brake operating member.
    • 用于制动装置的真空增压装置在需要时积极地降低节流阀的打开程度,同时可以有效地防止节流阀的开度不必要的降低。 真空助力器的第一压力室在节流阀的下游侧连接到发动机的进气管。 真空助力器的第二压力室可选择性地连接到第一压力室和大气中的一个。 真空助力器基于第一压力室和第二压力室之间的压力差来增加施加到制动操作构件的操作力。 节气门开度控制装置通过控制节流阀的开度来控制第一压力室中的负压。 节气门开度控制装置仅在第一压力室中的负压小于由与第一压力室相关的操作力相关量确定的预定必要负压时限制节气门的打开程度 施加到制动操作构件的操作力。
    • 7. 发明申请
    • THERMOSTABLE POLYMERASES HAVING ALTERED FIDELITY AND METHODS OF IDENTIFYING AND USING SAME
    • 具有变化的可塑性聚合物及其识别方法和使用方法
    • US20090004656A1
    • 2009-01-01
    • US11962067
    • 2007-12-20
    • Lawrence A. LoebLeroy HoodMotoshi Suzuki
    • Lawrence A. LoebLeroy HoodMotoshi Suzuki
    • C12Q1/68C12Q1/48C12N9/10C12N15/11C12P19/34
    • C12N9/1252C12Q1/6858C12Q1/6883
    • The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.
    • 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如,本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸,例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。
    • 8. 发明授权
    • File management apparatus and method
    • 文件管理装置和方法
    • US07020664B1
    • 2006-03-28
    • US09723972
    • 2000-11-28
    • Tomoko YamaguchiToshio MizunoMotoshi Suzuki
    • Tomoko YamaguchiToshio MizunoMotoshi Suzuki
    • G06F12/00
    • G06F17/30067
    • Upon receipt of a segment name obtainment request, the segment name generation unit 104 generates a segment name composed of (1) a file name of a file to which the segment belongs and (2) a number indicating a serial number of the segment in the file. Upon receipt of an access request specifying a segment name, the segment identification unit 119 refers to the segment name to detect the file name and the serial number of the segment in the detected file. The segment position detection unit 106 then refers to the file information storage unit 102 and the segment information storage unit 103 to detect a position where a segment is to be accessed.
    • 在接收到段名称获取请求时,段名称生成单元104生成由(1)段所属的文件的文件名构成的段名,(2)表示段的名称的序号, 文件。 在接收到指定段名称的访问请求时,段识别单元119参考段名来检测检测文件中的段的文件名和序列号。 然后,段位置检测单元106参考文件信息存储单元102和段信息存储单元103以检测段被访问的位置。
    • 9. 发明授权
    • Thermostable polymerases having altered fidelity and method of identifying and using same
    • 具有改变的保真度的热稳定性聚合酶和鉴定和使用它们的方法
    • US06395524B2
    • 2002-05-28
    • US08978806
    • 1997-11-26
    • Lawrence A. LoebLeroy HoodMotoshi Suzuki
    • Lawrence A. LoebLeroy HoodMotoshi Suzuki
    • C12N910
    • C12N9/1252C12Q1/6858C12Q1/6883
    • The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.
    • 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如,本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸,例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。