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    • 4. 发明申请
    • LIQUID SUPPLY DRIVE MECHANISM USING OSMOTIC PUMP AND MICROCHIP HAVING THE LIQUID SUPPLY DRIVE MECHANISM
    • 液体供应驱动机构使用OSMOTIC PUMP和具有液体供应驱动机构的MICROCHIP
    • US20100254832A1
    • 2010-10-07
    • US12742078
    • 2008-11-06
    • Takashi Nakazawa
    • Takashi Nakazawa
    • F04B47/08
    • B01L3/50273B01L2400/046B01L2400/0472F04B43/10
    • Provided is a microchip having a liquid supply mechanism which can supply a solution in a micro flow path with a simple construction in which a drive source utilizing an osmotic pressure is arranged within the microchip and which controls the osmotic pressure by a control unit utilizing temperature or the like, whereby it is possible to effect drive control such as intermittent driving and continuous driving at a fixed velocity. The liquid supply mechanism using osmotic pressure includes an osmotic pump utilizing the osmotic pressure of a liquid filling chambers separated from each other by a semipermeable membrane, and a unit which varies the osmotic pressure by changing a condition of the solution in at least one of the chambers on the basis of a timing of a pumping operation.
    • 本发明提供一种具有液体供给机构的微芯片,该液体供给机构能够以简单的结构向微流路供给溶液,在该结构中,使用渗透压的驱动源配置在微芯片内,利用温度控制单元控制渗透压, 从而可以以固定速度实现间歇驱动和连续驱动等驱动控制。 使用渗透压的液体供给机构包括使用通过半透膜隔开的液体填充室的渗透压的渗透泵,以及通过在至少一个中的至少一个中改变溶液的状态来改变渗透压的单元 基于泵送操作的定时。
    • 6. 发明授权
    • Method for selectively collecting N-terminal peptide fragment of protein
    • 选择性收集蛋白质N-末端肽片段的方法
    • US07041472B2
    • 2006-05-09
    • US10739111
    • 2003-12-19
    • Shigemi NoriokaNorikazu UeyamaTaka-aki OkamuraTakashi NakazawaMinoru YamaguchiEiji Ando
    • Shigemi NoriokaNorikazu UeyamaTaka-aki OkamuraTakashi NakazawaMinoru YamaguchiEiji Ando
    • C12P21/06
    • C12P21/06
    • The present invention provides a method for selectively collecting the N-terminal peptide fragments of a protein of interest whether or not the protein of interest is modified on the N-terminus. A method for selectively collecting the N-terminal peptide fragment of a protein, comprising: a protection step (1) of protecting side chain-amino groups of amino acid residues containing side chain-amino groups of a protein of interest to obtain a protected protein protected on the side chain-amino groups; a fragmentation step (2) of cleaving the protected protein into one N-terminal peptide fragment (a) containing the N-terminus of the peptide of interest and one or more of peptide fragments (b) other than the N-terminal peptide fragment (a); and a separation step (3) of separating the N-terminal peptide fragment (a) from the other peptide fragments (b) by selectively eluting the N-terminal peptide fragment (a) based on the difference in their reactivity or affinity to substrate, wherein the selective elution is achieved either by allowing the other peptide fragments (b) to bind to the substrate while allowing the N-terminal peptide fragment (a) to elute, or by allowing the N-terminal peptide fragment (a) to bind to the substrate while allowing the other peptide fragments (b) to elute and subsequently eluting the bound N-terminal peptide fragment (a).
    • 本发明提供了一种用于选择性收集目的蛋白质的N-末端肽片段的方法,无论目的蛋白质是否在N末端被修饰。 一种用于选择性收集蛋白质的N-末端肽片段的方法,包括:保护步骤(1)保护含有目的蛋白质的侧链 - 氨基的氨基酸残基的侧链氨基以获得受保护的蛋白质 保护侧链氨基; 将保护的蛋白质切割成包含目标肽的N末端的一个N-末端肽片段(a)和除了N-末端肽片段之外的一个或多个肽片段(b)的片段化步骤(2) 一个); 以及通过基于它们对底物的反应性或亲和力的差异来选择性地洗脱N末端肽片段(a),从而将N-末端肽片段(a)与其它肽片段(b)分离的分离步骤(3) 其中所述选择性洗脱通过使其它肽片段(b)与底物结合同时允许N-末端肽片段(a)洗脱,或通过使N-末端肽片段(a)与 同时允许其他肽片段(b)洗脱并随后洗脱结合的N-末端肽片段(a)。
    • 8. 发明授权
    • Apparatus and a method for controlling an engine
    • 用于控制发动机的装置和方法
    • US06879905B2
    • 2005-04-12
    • US10329785
    • 2002-12-27
    • Takahisa KosekiTetsuya IwasakiTakashi NakazawaMasahiko Yuya
    • Takahisa KosekiTetsuya IwasakiTakashi NakazawaMasahiko Yuya
    • F02D45/00F02N11/08F02D41/06
    • F02N11/0848F02D2200/1012F02N2200/022F02N2200/063
    • Engine cranking control is ended when it is detected that the battery voltage for driving the starter is lowered to at least as low as a starter drive judgment value and thereafter, returns to at least a starter stop judgment value during the engine cranking control that is started on condition that an engine is rotating. A start for driving a starter and a stop of driving the starter can be distinguished based on battery voltage where the battery voltage recovers due to a rapid reduction of consuming current and a rapid increase of a generating current when a driver recognizes an engine combustion completion and stops a starter driving. As a result, on increase of an engine friction, the cranking control is accurately ended, preventing deterioration of a fuel economy and excess exhaust gas emission.
    • 当检测到用于驱动起动器的电池电压被降低至至少与起动器驱动判定值一样低时,发动机起动控制结束,然后在启动的发动机起动控制期间至少返回起动器停止判定值 在发动机旋转的条件下。 驱动启动器和停止启动器的开始可以基于电池电压来区分,其中电池电压由于消耗电流的快速减少而恢复,并且当驾驶员识别发动机燃烧完成时快速增加发电电流, 停止起动机驾驶。 结果,在增加发动机摩擦的情况下,起动控制被准确地结束,防止燃料经济性的劣化和废气排放过多。
    • 9. 发明授权
    • Inhibitor switch
    • 抑制开关
    • US06693249B2
    • 2004-02-17
    • US10123430
    • 2002-04-17
    • Tsutomu WatadaTakashi Nakazawa
    • Tsutomu WatadaTakashi Nakazawa
    • H01H2118
    • H01H21/18F16H59/105H01H11/06H01H19/58H01H2011/067H01H2300/02
    • An inhibitor switch is used to correctly perform the detection of speed change positions. This inhibitor switch has a terminal block with fixed contacts and a moving block which is mounted and fitted onto a manual shaft of an automatic transmission, has a signaling contact and large capacity current-carrying contacts, and is rotatable relative to the terminal block. This inhibitor switch is used for electrically detecting a speed change position of the automatic transmission on the basis of a relative rotating positional relationship of the fixed contacts and the signaling contact in accordance with the rotation of the manual shaft. The moving block, which is mounted on the manual shaft, has a boss portion formed of a resin and a contact support portion whereon the signaling contact and large capacity current-carrying contacts are mounted. The contact support portion is formed of metal and the signaling contact is joined to the contact support portion by caulking.
    • 使用抑制开关来正确地执行速度变化位置的检测。 该抑制开关具有带固定触点的端子块和安装并安装在自动变速器的手动轴上的移动块,具有信号触点和大容量载流触点,并且可相对于端子块旋转。 该抑制开关用于根据手动轴的旋转,根据固定触点和信号触点的相对旋转位置关系来电气地检测自动变速器的变速位置。 安装在手动轴上的移动块具有由树脂形成的凸起部分和安装信号触头和大容量载流触点的触点支撑部分。 接触支撑部分由金属形成,信号触点通过铆接连接到接触支撑部分。
    • 10. 发明授权
    • Method of manufacturing an active matrix panel
    • 制造有源矩阵面板的方法
    • US6136625A
    • 2000-10-24
    • US111551
    • 1998-07-07
    • Takashi Nakazawa
    • Takashi Nakazawa
    • H01L29/786G02F1/136G02F1/1368H01L21/28H01L21/336H01L27/12H01L29/49H01L29/78H01L21/00
    • H01L29/66757H01L21/28079H01L29/4908H01L29/78621Y10S257/90
    • In the formation and structure of a thin film transistor (TFT), an insulator is formed to cover the surface of the transistor gate electrode, which electrode is separated from an underlying semiconductor layer, having defined source, drain and channel regions, by a gate insulating layer. The overlying gate insulator is formed by anodic oxidation of the gate electrode metal. The formation of the gate insulator thickness and its lateral offset, .DELTA.L, which is defined as the lateral spatial separation between the gate electrode and the source or drain region, can be accurately controlled by the gate electrode anodic oxidation process to provide a reliably and reproducible low OFF current, I.sub.OFF, resulting in a TFT that provides for a large I.sub.ON /I.sub.OFF ratio useful in large area applications wherein electrical charge is required, such as, liquid crystal displays and memory integrated circuits. Preferably, the metal gate electrode is subjected to anodic oxidation at a voltage within the range of between approximately 150 V to 250 V achieving a lateral offset, .DELTA.L, in the range of approximately 100 nm to 200 nm.
    • 在薄膜晶体管(TFT)的形成和结构中,形成绝缘体,以覆盖晶体管栅电极的表面,该电极与底层半导体层分离,具有限定的源极,漏极和沟道区,由栅极 绝缘层。 上覆栅极绝缘体通过栅电极金属的阳极氧化形成。 通过栅电极阳极氧化工艺可以精确地控制栅极绝缘体厚度及其横向偏移量DELTA L的形成,其定义为栅极和源极或漏极区域之间的横向空间间隔,以提供可靠和可靠的 可重现的低关断电流IOFF,导致提供可用于需要电荷的大面积应用(例如液晶显示器和存储器集成电路)中的大的ION / IOFF比率的TFT。 优选地,金属栅电极在大约150V至250V之间的电压下进行阳极氧化,实现了在大约100nm至200nm的范围内的横向偏移量DELTA L。