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1. 发明授权

US06539244B2 Automatic electrophysiological measuring apparatus/method 失效
标题翻译：自动电生理测量仪器/方法
公开(公告)号：US06539244B2
公开(公告)日：2003-03-25
申请号：US09740817
申请日：2000-12-21
申请人： Hirokazu Kato , Jun Otomo , Sayuri Nomura , Tomoko Takeshita , Sakae Saito , Shokichi Matsunami , Noboru Moriya
发明人： Hirokazu Kato , Jun Otomo , Sayuri Nomura , Tomoko Takeshita , Sakae Saito , Shokichi Matsunami , Noboru Moriya
IPC分类号： A61B505
CPC分类号： G01N33/48728
摘要： An automatic electrophysiological measuring apparatus to automatically penetrate a glass electrode(s) into the membrane of a Xenopus Oocyte to hold the membrane potential and to automatically measure reactions to the administration of a medicine is to be provided. A glass electrode 103 in the air is moved toward a Xenopus Oocyte 601 held in a cell. Changes in the potential states of the glass electrodes are picked up by a control computer to distinguish the relative positions of the glass electrode 102 and the Xenopus Oocyte 601, and the sequence of electrophysiological measurement so as to penetrate the glass electrode 102 into the Xenopus Oocyte 601, hold the membrane potential and automatically administer the medicine.
摘要翻译：提供一种自动电生理测量装置，用于将玻璃电极自动穿透到非洲爪蟾卵母细胞的膜中以保持膜电位并且自动测量对药物施用的反应。空气中的玻璃电极103朝向保持在电池中的爪蟾卵母细胞601移动。由控制计算机拾取玻璃电极的电位状态的变化，以区分玻璃电极102和非洲爪蟾卵母细胞601的相对位置，以及电生理测量序列，以便穿透玻璃电极102进入非洲爪蟾卵母细胞 601，保持膜电位并自动管理药物。

2. 发明授权

US06470201B2 Automatic electrophysiological measuring apparatus / method 失效
标题翻译：自动电生理测量仪器/方法
公开(公告)号：US06470201B2
公开(公告)日：2002-10-22
申请号：US09799119
申请日：2001-03-06
申请人： Hirokazu Kato , Jun Otomo , Sayuri Nomura , Tomoko Takeshita , Sakae Saito , Shokichi Matsunami , Noboru Moriya
发明人： Hirokazu Kato , Jun Otomo , Sayuri Nomura , Tomoko Takeshita , Sakae Saito , Shokichi Matsunami , Noboru Moriya
IPC分类号： A61B505
CPC分类号： G01N33/48728
摘要： An automatic electrophysiological measuring apparatus to automatically penetrate a glass electrode(s) into the membrane of a Xenopus Oocyte to hold the membrane potential and to automatically measure reactions to the administration of a medicine is to be provided. A glass electrode 103 in the air is moved toward a Xenopus Oocyte 601 held in a cell. Changes in the potential states of the glass electrodes are picked up by a control computer to distinguish the relative positions of the glass electrode 102 and the Xenopus Oocyte 601 and the sequence of electrophysiological measurement so as to penetrate the glass electrode 102 into the Xenopus Oocyte 601, hold the membrane potential and automatically administer the medicine.
摘要翻译：本发明提供一种自动电生理测量装置，用于将玻璃电极自动穿透到非洲爪蟾卵母细胞膜以保持膜电位并自动测量药物施用反应。空气中的玻璃电极103 移动到一个牢房中的非洲爪蟾卵母细胞601。通过控制计算机拾取玻璃电极的电位状态的变化，以区分玻璃电极102和非洲爪蟾卵母细胞601的相对位置和电生理测量序列，以便穿透玻璃电极102进入非洲爪蟾卵母细胞601 ，保持膜电位并自动管理药物。

3. 发明授权

US06593129B1 Apparatus for microinjection of sample into amphibian oocytes 失效
标题翻译：用于将样品显微注射到两栖动物卵母细胞中的装置
公开(公告)号：US06593129B1
公开(公告)日：2003-07-15
申请号：US09666530
申请日：2000-09-20
申请人： Tomoko Takeshita , Jun Otomo , Sayuri Nomura , Shokichi Matsunami , Noboru Moriya , Sakae Saito
发明人： Tomoko Takeshita , Jun Otomo , Sayuri Nomura , Shokichi Matsunami , Noboru Moriya , Sakae Saito
IPC分类号： C12M100
CPC分类号： C12M23/50 , C12M35/00
摘要： An apparatus for microinjection of samples into amphibian oocytes, comprising a tray for holding a plurality of the amphibian oocytes, an injection needle for injecting a sample into the said amphibian oocytes, a driving means for moving a relative position between the said tray and the said injection needle and a controlling means for controlling the said movement by imputing a depth of the said injection needle for the said tray or the said amphibian oocytes in the injection of the sample, and injecting the sample into the said amphibian oocytes at the said depth. According to the present invention, the sample can be injected into the amphibian oocyte with constant depth.precisely and quality of oocyte or a positional site of needle injection can be recorded as the information.
摘要翻译：一种用于将样品显微注射到两栖动物卵母细胞中的装置，包括用于保持多个两栖动物卵母细胞的托盘，用于将样品注入所述两栖动物卵母细胞的注射针;驱动装置，用于使所述托盘和所述注射针和控制装置，用于通过在注射样品时插入所述托盘或所述两栖动物卵母细胞的所述注射针的深度来控制所述运动，以及在所述深度将样品注入所述两栖动物卵母细胞。根据本发明，可以将样品以恒定的深度精确地注入到两栖动物卵母细胞中，并且可以记录卵母细胞的质量或针注射的位置位置作为信息。

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