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1. 发明授权

US5591589A Process for separating and measuring glycoprotein 失效
标题翻译：糖蛋白分离和测定方法
公开(公告)号：US5591589A
公开(公告)日：1997-01-07
申请号：US340299
申请日：1994-11-14
申请人： Hideo Katoh , Kenji Nakamura , Shinji Satomura
发明人： Hideo Katoh , Kenji Nakamura , Shinji Satomura
IPC分类号： G01N33/574 , G01N33/68 , G01N33/53
CPC分类号： G01N33/6803 , G01N33/57469 , G01N33/57476 , Y10S435/973 , Y10S436/827
摘要： The degree of a structure change of the sugar chain of a glycoprotein caused by a disease can be measured with high precision rapidly by separating and measuring two or more forms of glycoproteins which are different in sugar chain structure but have substantially the same protein structure, by using a combination of a lectin capable of recognizing the specific sugar chain structure of at least one of these glycoprotein analytes to be measured, and a first antibody which has a property of binding to all the glycoprotein analytes but is kept from binding to glycoprotein analyte(s) having the lectin attached thereto; and separating and measuring glycoprotein analyte(s) having the first antibody attached thereto and glycoprotein analyte(s) having no first antibody attached thereto, by utilizing differences in properties between the former glycoprotein analyte(s) and the latter glycoprotein analyte(s).
摘要翻译：由疾病引起的糖蛋白的糖链的结构变化程度可以通过分离和测定两种或多种形式的糖链结构不同但具有基本相同的蛋白质结构的糖蛋白，通过快速测定，由使用能够识别待测量的这些糖蛋白分析物中的至少一种的特定糖链结构的凝集素的组合和具有结合所有糖蛋白分析物但不与糖蛋白分析物结合的性质的第一抗体（ s）具有凝集素附着; 并且通过利用前一种糖蛋白分析物和后一种糖蛋白分析物之间的特性差异来分离和测量具有附着于其上的第一抗体的糖蛋白分析物和与其无关的第一抗体的糖蛋白分析物。

2. 发明授权

US06828417B2 Polypeptide and process for measuring living body components using the same 失效
标题翻译：多肽和使用其测量活体成分的方法
公开(公告)号：US06828417B2
公开(公告)日：2004-12-07
申请号：US09883394
申请日：2001-06-19
申请人： Nobuko Imajo , Yukari Yamagata , Hideo Katoh , Shinji Satomura , Kenji Nakamura
发明人： Nobuko Imajo , Yukari Yamagata , Hideo Katoh , Shinji Satomura , Kenji Nakamura
IPC分类号： C07K700
CPC分类号： C07K7/06 , C07K1/1075 , C07K7/02 , C07K7/08 , Y02P20/55 , Y10S436/824
摘要： A living body component in a sample derived from a living body can be rapidly and accurately measured by reacting the sample with a reagent comprising a combined product of an affinity substance and a polypeptide having at least three acid residues derived from a strong acid, separating the resulting complex by a method applying negative change such as using an anion-exchanger, and determining the amount of the analyte to be measured, on the basis of the amount of the complex or free combined product.
摘要翻译：通过使样品与包含亲和物质和具有至少3个来源于强酸的酸残基的多肽的组合产物的试剂反应来快速准确地测定来自活体的样品中的活体成分，通过使用阴离子交换剂的负变化的方法得到的复合物，以及基于复合物或游离组合产物的量确定待测量的分析物的量。

3. 发明授权

US06300079B1 Polypeptide and process for measuring living body components using the same 失效
标题翻译：多肽和使用其测量活体成分的方法
公开(公告)号：US06300079B1
公开(公告)日：2001-10-09
申请号：US08680855
申请日：1996-07-16
申请人： Nobuko Imajo , Yukari Yamagata , Hideo Katoh , Shinji Satomura , Kenji Nakamura
发明人： Nobuko Imajo , Yukari Yamagata , Hideo Katoh , Shinji Satomura , Kenji Nakamura
IPC分类号： G01N33538
CPC分类号： C07K7/06 , C07K1/1075 , C07K7/02 , C07K7/08 , Y02P20/55 , Y10S436/824
摘要： A living body component in a sample derived from a living body can be rapidly and accurately measured by reacting the sample with a reagent comprising a combined product of an affinity substance and a polypeptide having at least three acid residues derived from a strong acid, separating the resulting complex by a method applying negative change such as using an anion-exchanger, and determining the amount of the analyte to be measured, on the basis of the amount of the complex or free combined product.
摘要翻译：通过使样品与包含亲和物质和具有至少3个来源于强酸的酸残基的多肽的组合产物的试剂反应来快速准确地测定来自活体的样品中的活体成分，通过使用阴离子交换剂的负变化的方法得到的复合物，以及基于复合物或游离组合产物的量确定待测量的分析物的量。

4. 发明授权

US5677194A Process for rapid measurement of trace components 失效
标题翻译：快速测量微量元素的过程
公开(公告)号：US5677194A
公开(公告)日：1997-10-14
申请号：US309967
申请日：1994-09-20
申请人： Shinji Satomura , Hideo Katoh , Kenji Nakamura , Kazunari Hirayasu
发明人： Shinji Satomura , Hideo Katoh , Kenji Nakamura , Kazunari Hirayasu
IPC分类号： G01N33/537 , G01N33/538 , G01N33/53 , G01N33/536
CPC分类号： G01N33/538 , Y10S436/805 , Y10S436/824 , Y10S436/827
摘要： Trace components in samples can be measured rapidly with high precision by applying interaction between an analyte to be measured and an affinity substance and using a membrane having specific separating capability.
摘要翻译：样品中的痕量组分可以通过应用待测分析物和亲和物质之间的相互作用并使用具有特定分离能力的膜，以高精度快速测量。

5. 发明授权

US5571729A Process for separating complex 失效
标题翻译：分离复合物的方法
公开(公告)号：US5571729A
公开(公告)日：1996-11-05
申请号：US354264
申请日：1994-12-12
申请人： Kenji Nakamura , Taizo Hara , Hideo Katoh , Shinji Satomura
发明人： Kenji Nakamura , Taizo Hara , Hideo Katoh , Shinji Satomura
IPC分类号： G01N33/538 , G01N33/573
CPC分类号： G01N33/538 , Y10S436/824
摘要： This invention is intended to provide a process by which a complex formed by the interaction between one or more analytes to be measured and affinity substance can be more effectively separated from substances existing together therewith which tend to affect the detection of the complex, for example, free affinity substance, by using high pressure liquid chromatography (HPLC); and a process for measuring a trace component by utilizing said separating process.This invention is characterized in that a substance which has been modified with a substance capable of changing properties of the complex (a separation-improving substance) and has affinity for the complex is attached to the complex. Because of this characteristic, the invention is effective in that the position of elution of said complex in the HPLC can be freely controlled. Furthermore, it is markedly effective in that the process for measuring a trace component by utilizing the separating process of this invention makes it possible to carry out the measurement with higher precision as compared with conventional measuring processes using HPLC.
摘要翻译：本发明旨在提供一种方法，通过该方法可以将待测量的一种或多种待分析物与亲和物质之间的相互作用形成的复合物与其中存在的物质更有效地分离，这些物质倾向于影响复合物的检测，例如，游离亲和性物质，通过使用高压液相色谱（HPLC）; 以及通过利用所述分离过程来测量痕量组分的方法。本发明的特征在于，将具有能够改变复合物（分离改善物质）性质并对复合物具有亲和性的物质进行了改性的物质附着于该络合物。由于该特征，本发明的效果在于可以自由地控制HPLC中所述络合物的洗脱位置。此外，通过利用本发明的分离方法测量痕量组分的方法与使用HPLC的常规测量方法相比，可以更高精度地进行测量。

6. 发明授权

US5780247A Process for separating and measuring trace components 失效
标题翻译：分离和测量痕量组分的方法
公开(公告)号：US5780247A
公开(公告)日：1998-07-14
申请号：US488009
申请日：1995-06-07
申请人： Shinji Satomura , Kenji Nakamura , Shuji Matuura
发明人： Shinji Satomura , Kenji Nakamura , Shuji Matuura
IPC分类号： C12Q1/40 , C12Q1/48 , G01N33/543 , G01N33/76 , G01N33/574
CPC分类号： G01N33/54306 , C12Q1/40 , C12Q1/48 , G01N33/76 , Y10S435/971 , Y10S435/973 , Y10S436/824 , Y10S436/827
摘要： Two or more analytes having the same action, or having different actions in spite of their similar structures, or two or more analytes having the same action and the same detectable chemical characteristics, in samples derived from living bodies, etc., can be measured rapidly and easily by forming one or more complexes with one or more affinity substances, separating the complexes by using high pressure liquid chromatography, followed by measurement of the amount of an affinity substance or one of the analytes.
摘要翻译：具有相同作用的两种或更多种分析物，或者具有相似结构的不同作用，或者来自活体等的样品中具有相同作用和相同可检测化学特性的两种或更多种分析物可以快速测量并且通过与一种或多种亲和物质形成一种或多种配合物，通过使用高压液相色谱法分离复合物，然后测量亲和物质或分析物之一，容易地。

7. 发明授权

US07842175B2 Electrophoresis 有权
标题翻译：电泳
公开(公告)号：US07842175B2
公开(公告)日：2010-11-30
申请号：US10472753
申请日：2002-04-03
申请人： Tomohisa Kawabata , Kenji Nakamura , Shinji Satomura
发明人： Tomohisa Kawabata , Kenji Nakamura , Shinji Satomura
IPC分类号： G01N27/447
CPC分类号： G01N33/532 , C12Q1/68 , G01N27/44726 , C12Q2565/125 , C12Q2563/179
摘要： The invention relates to a method for separating a target for measurement utilizing electrophoresis, particularly capillary electrophoresis efficiently in high sensitivity and in a short period of time. It also relates to a method for measuring said target separated by said method for separation. The invention provides a method for separating a target for measurement and a method for measuring said target separated by said method for separation, characterized by using a substance to which is bound a nucleic acid chain labeled by a marker and which has an affinity for said target for measurement.
摘要翻译：本发明涉及一种利用电泳，特别是高灵敏度和短时间内有效毛细管电泳分离测定靶的方法。本发明还涉及一种用于分离由所述分离方法分离的所述靶的测量方法。本发明提供了一种用于分离测量目标的方法和由所述分离方法分离的用于测量所述靶的方法，其特征在于使用结合由标记物标记的核酸链的物质，所述核酸链对所述靶标具有亲和力用于测量。

8. 发明授权

US6156195A Method for separating components in liquid specimen and apparatus used in said method 失效
公开(公告)号：US6156195A
公开(公告)日：2000-12-05
申请号：US48327
申请日：1998-03-26
申请人： Kenji Nakamura , Shinji Satomura , Masayoshi Hayashi , Hideyoshi Arashima , Tetsushi Okuyama , Nobuhiro Hanafusa , Koji Tanimizu
发明人： Kenji Nakamura , Shinji Satomura , Masayoshi Hayashi , Hideyoshi Arashima , Tetsushi Okuyama , Nobuhiro Hanafusa , Koji Tanimizu
IPC分类号： B01D15/08 , G01N30/16 , G01N30/24 , G01N30/40 , G01N30/88
CPC分类号： G01N30/40 , B01D15/1807 , B01D15/14 , G01N2030/402 , G01N2030/407 , G01N30/16 , G01N30/24 , G01N30/88
摘要： The object of the present invention is to provide a method for separating components in a liquid specimen and an apparatus used in said method, by which it is possible to avoid carryover even when the specimen injector is not washed using washing solution and also to avoid the increase of inner pressure in column of the separation device or ineffective separating operation even when many liquid specimens are separated by many times using the same separation device. In the present invention, the liquid specimen is introduced into the separation device from a direction opposite to eluting direction of the separation device which retains an adsorbent, and by separating and eluting the specimen from eluting outlet together with the eluant, trace quantity of insoluble substances and the specimen remained on the specimen introducing passage can be removed by elution procedure. As a result, it is possible to avoid carryover, to prevent the increase of inner pressure in the column of the separation device and to prevent ineffective separating operation caused by clogging of the column.

9. 发明授权

US5545530A Process for measuring analyte in sample 失效
标题翻译：测量样品中分析物的方法
公开(公告)号：US5545530A
公开(公告)日：1996-08-13
申请号：US442986
申请日：1995-05-17
申请人： Shinji Satomura , Kenji Nakamura , Tokuji Ikenaka , Kaoru Omichi
发明人： Shinji Satomura , Kenji Nakamura , Tokuji Ikenaka , Kaoru Omichi
IPC分类号： G01N30/02 , G01N30/06 , G01N30/88 , G01N33/537 , G01N33/538 , G01N33/53
CPC分类号： G01N33/537 , G01N33/538 , G01N2030/027 , G01N30/06 , Y10S435/803 , Y10S435/971 , Y10S436/812 , Y10S436/824
摘要： The amount of an analyte in a sample derived from a living sample is measured by reacting the analyte with an excess of a substance having affinity for the analyte, followed by separation of complex by high pressure liquid chromatography and measurement using a linear calibration curve representing the peak area values associated with known concentrations of analyte.
摘要翻译：通过使分析物与过量的对分析物具有亲和力的物质反应，然后通过高压液相色谱法分离复合物，并使用表示所述分析物的线性校准曲线进行测量来测量来自活体样品的样品中的分析物的量与已知浓度的分析物相关的峰面积值。

10. 发明授权

US6149818A Method for separating components in liquid specimen and apparatus used in said method 失效
标题翻译：用于分离液体试样中的组分的方法和用于所述方法的装置
公开(公告)号：US6149818A
公开(公告)日：2000-11-21
申请号：US386149
申请日：1999-08-31
申请人： Kenji Nakamura , Shinji Satomura , Masayoshi Hayashi , Hideyoshi Arashima , Tetsushi Okuyama , Nobuhiro Hanafusa , Koji Tanimizu
发明人： Kenji Nakamura , Shinji Satomura , Masayoshi Hayashi , Hideyoshi Arashima , Tetsushi Okuyama , Nobuhiro Hanafusa , Koji Tanimizu
IPC分类号： B01D15/08 , G01N30/16 , G01N30/24 , G01N30/40 , G01N30/88
CPC分类号： G01N30/40 , B01D15/1807 , B01D15/14 , G01N2030/402 , G01N2030/407 , G01N30/16 , G01N30/24 , G01N30/88
摘要： The object of the present invention is to provide a method for separating components in a liquid specimen and an apparatus used in said method, by which it is possible to avoid carryover even when the specimen injector is not washed using washing solution and also to avoid the increase of inner pressure in column of the separation device or ineffective separating operation even when many liquid specimens are separated by many times using the same separation device. In the present invention, the liquid specimen is introduced into the separation device from a direction opposite to eluting direction of the separation device which retains an adsorbent, and by separating and eluting the specimen from eluting outlet together with the eluant, trace quantity of insoluble substances and the specimen remained on the specimen introducing passage can be removed by elution procedure. As a result, it is possible to avoid carryover, to prevent the increase of inner pressure in the column of the separation device and to prevent ineffective separating operation caused by clogging of the column.
摘要翻译：本发明的目的是提供一种用于分离液体试样中的组分和所述方法中使用的装置的方法，即使当使用洗涤溶液不洗涤试样注射器时也可以避免携带，并且避免即使使用相同的分离装置多次分离多个液体样品，分离装置的塔内部压力增加或分离操作无效。在本发明中，将液体试样从与保留吸附剂的分离装置的洗脱方向相反的方向引入分离装置，通过从洗脱液与洗脱液一起分离和洗脱试样，追踪微量的不溶物残留在样品导入通道上的样品可以通过洗脱程序除去。结果，可以避免残留，以防止分离装置的塔中的内压增加，并且防止由于柱的堵塞而导致的无效的分离操作。

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